USDA-ARS Western Wheat Quality Laboratory. Genotype & Environment Study 6-Year Summary

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1 USDA-ARS Western Wheat Quality Laboratory Genotype & Environment Study 6-Year Summary Crop Years Douglas A. Engle and Craig F. Morris with the assistance of Brady P. Carter Washington State University Wheat Quality Specialist December 30, 2002 This is a Progress Report of cooperative investigations of the milling and baking characteristics of current commercial varieties and new germplasm of wheat grown in the Western United States. Interpretation of the data may be changed with further experimentation; therefore, data and results in this report are not for publication, display, or distribution without prior written approval of the Agricultural Research Service, USDA and the cooperating agencies concerned.

2 EXECUTIVE SUMMARY This report compiles and summarizes the first six years of a study aimed at profiling the quality of the leading commercial wheat varieties and advanced experimental breeding lines in or nearing production throughout the Columbia River-PNW export region. Grain samples were obtained from university extension trials with multiple sites (nurseries) per crop year. In these nurseries, varieties were grown side-by-side under identical management and weather conditions. Milling, baking and other end-use quality traits were determined at the USDA-ARS Western Wheat Quality Laboratory (WWQL) with the assistance of Brady P. Carter, Washington State University Wheat Quality Specialist. Statistical analyses (t-tests) were conducted to compare each variety against a selected check variety. The resultant t-scores were used to rate varieties according to their relative overall quality. Similarly, individual environments were statistically analyzed to assess both the relative contribution of this factor to quality, as well as to examine the effect of location on producing high quality grain. The aim of this study was to identify superior quality varieties and identify which regions they may be best produced in. Mention of trademark or proprietary product does not constitute a guarantee or warranty of a product by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products that may also be suitable. This article is in the public domain and not copyrightable. Page 2

3 Table of Contents Executive Summary Table of contents Introduction Calculation of Summary t-scores Tables of summary t-scores Tables of variety t-scores Tables of varieties and locations by crop year Organization and description of the files on CD-ROM Acknowledgments Page 3

4 Introduction This report compiles and summarizes the first six years of a study aimed at profiling the quality of the leading commercial wheat varieties and advanced experimental breeding lines in or nearing production throughout the Columbia River-PNW export region. The Washington, Idaho and Oregon Wheat Commissions each supported financially the analysis of samples from their respective states. Grain samples were obtained from university extension trials with multiple, individual sites (nurseries) per crop year. In these nurseries, varieties were grown side-by-side under identical management and weather conditions. Milling, baking and other end-use quality traits were determined at the USDA-ARS Western Wheat Quality Laboratory (WWQL) with the assistance of Brady P. Carter, Washington State University Wheat Quality Specialist. Statistical analyses (t-tests) were conducted to compare each variety against a selected check variety. The resultant t-scores were used to rate varieties according to their relative overall quality. Check varieties were selected based on their prominence in production, extensive testing history at the WWQL, and an understanding of the relationship of the quality of the check to that of the market class or sub-class. Individual environments were statistically analyzed using Analysis of Variance (ANOVA) to assess both the relative contribution of the environment to quality, as well as to examine the effect of location on producing high quality grain. The aim of this study was to identify superior quality varieties and identify which regions they may be best produced in. NB: Many studies of this type analyze the genotype effect those differences ascribable to genetic differences among varieties, the environmental effects that variation ascribable to differences among geographical locations and crop years, and the interaction of these two the G-by-E (G X E). However, to conduct such a statistical analysis, replicate grain samples are required. In the present study, replication of variety samples in individual environments was foregone to contain the cost of the research. Consequently, the GXE interaction term has not been directly analyzed. Hence the name G&E. For the ANOVA, the data for each test variety is sub-setted individually along with that of the check variety, using only check variety nurseries wherein the test variety being analyzed appears, i.e. a balanced design, and there appears at least five observations of the test variety. The t-score is actually the statistic, Student s t (R.G.D. Steel, J.H. Torrie and D.A. Dickey, 1997, Principles and Procedures of Statistics: A Biometrical Approach, 3 rd ed., The McGraw-Hill Co., New York). Student s t is calculated by SAS using the two-tailed t-distribution and forcing the check variety to appear after the test variety in the analysis such that positive values of t indicate the test variety has a value greater than that of the check, negative values indicating that the test variety value is less than that of the check. For the individual t-scores (pages 10-15, no judgement has been made as to whether greater or lesser values are more or less desirable from a quality standpoint. In calculating the Summary t-scores (pages 6-9), this judgement has been applied, changing the sign of the weighting term as appropriate (see page 5). For the analyses here, which represent from 5 to 39 observations (4 to 38 degrees of freedom), the value of t at the P = 0.05 probability of a numerically lager value of t is to about A description of the materials and methods used for assessing end-use quality can be found at: Page 4

5 Calculation of Summary t-scores Minimum number of observations per test variety = 5 Check Varieties: Stephens, Rely, Alpowa, WPB 926, ID377s, and Finley. Soft White Winter Wheat Varieties Grain = (test weight *0.2) + (wheat protein * -0.8) Milling = (milling score * 0.6) + (break flour yield * 0.4) End-product = (cookie diam. * 0.8) + (mixo. abs. * -0.2) Overall = (grain * 0.1) + (milling * 0.4) + (end-product * 0.5) Soft White Winter Club Wheat Varieties Grain = (test weight *0.2) + (wheat protein * -0.8) Milling = (milling score * 0.6) + (break flour yield * 0.4) End-product = (cookie diam. * 0.6) + (mixo. abs. * -0.4) Overall = (grain * 0.1) + (milling * 0.4) + (end-product * 0.5) Soft White Spring Wheat Varieties Grain = (test weight *0.2) + (wheat protein * -0.8) Milling = (milling score * 0.6) + (break flour yield * 0.4) End-product = (cookie diam. * 0.8) + (mixo. abs. * -0.2) Overall = (grain * 0.1) + (milling * 0.4) + (end-product * 0.5) Hard Red and White Spring Wheat Varieties (Combined Analysis) Grain = (test weight *0.2) + (wheat protein * +0.8) Milling = (milling score * 1.0) End-product = (loaf vol. * 0.8) + (mixo. abs. * 0.2) Overall = (grain * 0.1) + (milling * 0.3) + (end-product * 0.6) Hard White Spring Wheat Varieties (Separate Analysis) Grain = (test weight *0.2) + (wheat protein * +0.8) Milling = (milling score * 1.0) End-product = (loaf vol. * 0.5) + (L24 Alkaline Noodle Color * 0.5) Overall = (grain * 0.05) + (milling * 0.3) + (end-product * 0.65) Hard Red and White Winter Wheat Varieties Grain = (test weight *0.2) + (wheat protein * +0.8) Milling = (milling score * 1.0) End-product = (loaf vol. * 0.8) + (mixo. abs. * 0.2) Overall = (grain * 0.1) + (milling * 0.3) + (end-product * 0.6) Page 5

6 Soft White Winter Wheat Variety Summary t-scores Variety Grain Milling End- Product Overall By Rank Overall ALBION BRUNDAGE ARS LEW JAIN 2.49 BASIN ID17113A 2.28 BEAMER FINCH 2.23 BRUNDAGE ROD 1.44 CASHUP CASHUP 1.24 DAWS HILL ELTAN HUBBARD 1.13 FINCH ID GT ELTAN 1.03 HILL MJ HUBBARD ID A 0.25 ID17113A WA ID BASIN 0.04 ID A STEPHENS 0.00 LAMBERT MOHLER LEW JAIN LAMBERT MACVICAR WA MADSEN ARS MJ BEAMER MJ GT MOHLER TUBBS OR MACVICAR ROD MJ STEPHENS OR TUBBS ALBION WA MADSEN WA WEATHERFORD WA WA WEATHERFORD WPB WPB DAWS Soft White Winter Club Wheat Variety Summary t-scores Variety Grain Milling End- Product Overall By Rank Overall ARS CHUKAR 3.71 BRUEHL HILLER 3.61 CHUKAR ARS CODA EDW IN 2.22 EDW IN TRES 0.76 HILLER BRUEHL 0.70 RELY TEMPLE 0.01 ROHDE RELY 0.00 TEMPLE CODA TRES ROHDE Page 6

7 Soft White Spring Wheat Variety Summary t-scores Variety Grain Milling End- Product Overall By Rank Overall ALPOWA VANNA 8.19 ALTURAS EDEN 6.68 BZ ZAK 5.70 CALORWA JUBILEE 5.57 CENTENNIAL WA CHALLIS CHALLIS 4.21 EDEN WA EDW ALL ALTURAS 3.70 FIELDER WA ID WHITEBIRD 3.61 JUBILEE WA PENAWAWA BZ POMERELLE POMERELLE 2.16 SPRITE WAKANZ 2.01 VANNA FIELDER 1.95 WA SPRITE 1.75 WA WA WA WAWAWAI 1.24 WA ID WA EDW ALL 1.21 WAKANZ CALORWA 1.13 WAWAWAI CENTENNIAL 1.09 WHITEBIRD ALPOWA 0.00 ZAK PENAWAWA Page 7

8 Hard Red and White Spring Wheat Variety Summary t-scores Variety Grain Milling End- Product Overall Variety Overall BUTTE TARA BZ W JEFFERSON 2.22 CO1955W KLASIC 1.94 CO1963W PLATA 1.90 EXPRESS HOLLIS 1.82 GMBR WPB GMBR WA HANK MACON 0.95 HOLLIS WA ID377S SPILLMAN 0.66 ID ID JEFFERSON SCARLET 0.63 KLASIC HANK 0.60 LOLO PRISTINE 0.40 MACON SX1502B 0.00 ML WPB NW# WA PLATA ZEKE PRISTINE WA SAXON CO1963W SCARLET WA SEEDEXHR SAXON SPILLMAN BZ W SX1501B BUTTE SX1502B EXPRESS SX1503B NW# TARA CO1955W WA ML WA GMBR WA LOLO WA WINSOME WA GMBR WINSOME SEEDEXHR WPB SX1501B WPB ID377S ZEKE SX1503B Page 8

9 Hard White Spring Wheat Variety Summary t-scores Variety Grain Milling End- Product Overall By Rank Overall BZ W MACON 2.48 CO1955W ML CO1963W WA GMBR LOLO 0.84 ID377S BZ W 0.84 KLASIC WINSOME 0.28 LOLO ID377S 0.00 MACON PLATA ML CO1955W PLATA KLASIC WA CO1963W WINSOME GMBR Hard Red and White Winter Wheat Variety Summary t-scores Variety Grain Milling End- Product Overall By Rank Overall BLIZZARD PILLAR 0.40 BOUNDARY FINLEY 0.00 BUCHANAN HATTON COLUMBIA BLIZZARD ESTICA WESTON FINLEY MORELAND GARY BUCHANAN HATTON Q MORELAND NUHORIZON NUFRONTIER NUFRONTIER NUHORIZON GARY PILLAR BOUNDARY Q WANSER Q RESIDENCE RESIDENCE COLUMBIA SEMPER SEMPER SYMPHONY Q WANSER SYMPHONY WESTON ESTICA Page 9

10 Soft White Winter Wheat Variety t-scores Break Flour Test NIR Wheat Single Kernel Flour Flour Flour Milling Flour Swelling Flour Mixo. Cookie Variety N Weight Hard. Protein Hard. Weight Yield Yield Ash Score Protein Volume RVA Abs. Diameter ALBION ARS BASIN BEAMER BRUNDAGE CASHUP DAWS ELTAN FINCH GT HILL HUBBARD ID17113A ID ID A LAMBERT LEWJAIN MACVICAR MADSEN MJ MJ MOHLER OR ROD STEPHENS TUBBS WA WA WA WEATHERFORD WPB Page 10

11 Soft White Winter Club Wheat Variety t-scores Break Test NIR Wheat Single Kernel Flour Flour Flour Milling Flour Mixo. Cookie Cake Variety N Weight Hard. Protein Hard. Weight Yield Yield Ash Score Protein Abs. Diameter Volume ARS BRUEHL CHUKAR CODA EDWIN HILLER RELY ROHDE TEMPLE TRES Page 11

12 Soft White Spring Wheat Variety t-scores Break Flour Test NIR Wheat Single Kernel Flour Flour Flour Milling Flour Swelling Flour Mixo. Cookie Variety N Weight Hard. Protein Hard. Weight Yield Yield Ash Score Protein Volume RVA Abs. Diameter ALPOWA ALTURAS BZ CALORWA CENTENNIAL CHALLIS EDEN EDWALL FIELDER ID JUBILEE PENAWAWA POMERELLE SPRITE VANNA WA WA WA WA WA WAKANZ WAWAWAI WHITEBIRD ZAK Page 12

13 Hard Red and White Spring Wheat Variety t-scores Bread Baking Test NIR Wheat Single Kernel Flour Flour Milling Flour Mixo. Water Loaf Crumb Variety N Weight Hard. Protein Hard. Weight Yield Ash Score Protein Abs. Abs. Volume Ranking BUTTE BZ W CO1955W CO1963W EXPRESS GMBR GMBR HANK HOLLIS ID377S ID JEFFERSON KLASIC LOLO MACON ML NW# PLATA PRISTINE SAXON SCARLET SEEDEXHR SPILLMAN SX1501B SX1502B SX1503B TARA WA WA Page 13

14 HRS & HWS, cont. Bread Baking Test NIR Wheat Single Kernel Flour Flour Milling Flour Mixo. Water Loaf Crumb Variety N Weight Hard. Protein Hard. Weight Yield Ash Score Protein Abs. Abs. Volume Ranking WA WA WA WINSOME WPB WPB ZEKE Hard White Spring Wheat Variety t-scores Bread Baking Alkaline Noodle Color Test NIR Wheat Single Kernel Flour Flour Milling Flour Flour Mixo. Water Loaf Crumb Brightness Variety N Weight Hard. Protein Hard. Weight Yield Ash Score Protein RVA Abs. Abs. Vol. Rank. 0 Hr 24 Hr Change BZ W CO1955W CO1963W GMBR ID377S KLASIC LOLO MACON ML PLATA WA WINSOME Page 14

15 Hard Red and White Winter Wheat Variety t-scores Bread Baking Test NIR Wheat Single Kernel Flour Flour Milling Flour Mixo. Water Loaf Crumb Variety N Weight Hard. Protein Hard. Weight Yield Ash Score Protein Abs. Abs. Volume Ranking BLIZZARD BOUNDARY BUCHANAN COLUMBIA ESTICA FINLEY GARY HATTON MORELAND NUFRONTIER NUHORIZON PILLAR Q Q RESIDENCE SEMPER SYMPHONY WANSER WESTON Page 15

16 Organization and Description of the Files on CD-ROM WWQL TDIFF 6 Year Summary.xls WWQL TDIFF 6 Year.xls G&E Variety List xls Report 6-Year G&E.wpd Report 6-Year G&E.pdf Summary t-scores, Excel spreadsheet (1 tab) t-scores, Excel spreadsheet (6 tabs) Tabulation of varieties and locations by crop year, Excel spreadsheet (4 tabs) This report, Word Perfect 10 document This report, Adobe Acrobat G&E Data & Program Files Raw Data Output HS DATA OUPUT.lst HW DATA OUTPUT.lst SS DATA OUPUT.lst SW DATA OUPUT.lst 4 files, raw data output from SAS, ASCII SAS GLM Code by Variety 136 files, SAS ANOVA GLM program code, ASCII HS TDIFF xxxxxxx.sas, where xxxxxxx = the variety name HW TDIFF xxxxxxx.sas, where xxxxxxx = the variety name HWS TDIFF xxxxxxx.sas, where xxxxxxx = the variety name SS TDIFF xxxxxxx.sas, where xxxxxxx = the variety name SW CLUB TDIFF xxxxxxx.sas, where xxxxxxx = the variety name SW TDIFF xxxxxxx.sas, where xxxxxxx = the variety name Page 24

17 SAS GLM Output by Variety HS TDIFF xxxxxxx.lst, where xxxxxxx = the variety name HW TDIFF xxxxxxx.lst, where xxxxxxx = the variety name HWS TDIFF xxxxxxx.lst, where xxxxxxx = the variety name SW TDIFF xxxxxxx.lst, where xxxxxxx = the variety name *136 files, SAS ANOVA GLM output, ASCII *These files contain the t-tests for variety means, and Duncan s Multiple Range Test mean comparisons for individual environments. SAS Permanent Data Sets gxennhs.sas7bdat, where NN = crop year gxennhw.sas7bdat, where NN = crop year gxennss.sas7bdat, where NN = crop year gxennsw.sas7bdat, where NN = crop year 24 files, permanent SAS data sets Location-Zone Coding 6 files, SAS program code for location-year environments, G&E_NN.sas, where NN = crop year includes Idaho and Oregon locations, ASCII Page 25

18 Acknowledgments Many thanks to the Cultivar Development Team at the USDA ARS Western Wheat Quality Laboratory: Mary Baldridge, Renee Engle, William Kelley, John Connett, Gail Jacobson, Skip King, Linhda Nguyen, and Carrie Oliver. Also, to Tracy Harris, WSU Wheat Quality Program. Eric Zakarison and Jeff Janosky (formerly), Washington Wheat Commission, were instrumental in moving this project forward and reducing vast quantities of technical information to simple numerical scores. John Burns and Pat Reisenaur, WSU Variety Testing; Steve Guy, University of Idaho; and Russ Karow, Oregon State University provided the samples for this study. Many thanks to them and to their crews. This project was partially funded by the Washington, Idaho, and Oregon state wheat commissions. Page 26

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