Hie-Joon Kim. Professor Emeritus Seoul National University. Experience. Representative Publications
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1 Hie-Joon Kim Professor Emeritus Seoul National University B.S. Chemistry, Seoul National University, Korea, 1970 Ph.D. Chemistry, University of Chicago, USA, 1977 Experience Professor, Department of Chemistry Seoul National University ( ) Chair Professor, Gwangju Institute of Science and Technology (2013-Present) SNU Teaching Award (2007) Representative Publications Philosophical Questions Scientific Answers (Thinking Power Books, 2012) Chemistry of Life, Chemistry for Life (Free Academy, 2009)
2 Chapter 1 Structure of DNA
3 INTRODUCTION Nature April 25, 1953 Molecular Structure of Nucleic Acids J. D. Watson and F.H.C. Crick Cavendish Laboratory, Cambridge
4 INTRODUCTION We wish to suggest a structure for the salt of deoxyribose nucleic acid (DNA). This structure has novel features which are of considerable biological interest. <source> _oe26di/aaaaaaahnq0/zlkzyfu-wi8/ s1600/watsoncrickdna.jpg
5 1-1 Pauling s model A structure for nucleic acid has already been proposed by Pauling and Corey. They kindly made their manuscript available to us in advance of publication. <source> img6bonds1.jpg
6 1-1 Pauling s model PNAS February 1953, Their model consists of three intertwined chains, with the phosphates near the fiber axis, and the bases on the outside. <source> png
7 1-1 Pauling s model In our opinion, this structure is unsatisfactory for two reasons: (1) We believe that the material which gives the X-ray diagram is the salt, not the free acid.
8 1-1 Pauling s model Without the acidic hydrogen atoms it is not clear what forces would hold the structure together, especially as the negatively charged phosphates near the fiber axis will repel each other.
9 1-1 Pauling s model (2) Some of the van der Waals distances appear to be too small. Johannes D. van der Waals Nobel Prize in Physics (1910)
10 1-2 Double Helix We wish to put forward a radically different structure for the salt of deoxyribose nucleic acid. This structure has two helical chains each coiled round the same axis.
11 1-2 Double Helix The bases are on the inside of the helix and the phosphates are on the outside. <source> iew/nucleotide.jpg/ /nucleotide.jpg
12 1-2 Double Helix There is a residue on each chain every 3.4 A in the z-direction. The distance of a phosphorus atom from the fiber axis is 10 A.
13 1-3 Base pair The novel feature of the structure is the manner in which the two chains are held together by the purine and pyrimidine bases. <source> Cartoon Guide to Genetics (Gonick)
14 1-3 Base pair They are joined together in pairs, a single base from one chain being hydrogen-bonded to a single base from the other chain, so that the two lie side by side with identical z-coordinates.
15 1-4 Base Sequence The sequence of bases on a single chain does not appear to be restricted in any way. l i f e s a l p h a b e t A C C G T A T G C C A T C T
16 1-4 Base Sequence However, if only specific pairs of bases can be formed, it follows that, if the sequence of bases on one chain is given, then the sequence on the other chain is automatically determined. A C C G T A T G C C A T C T T G G C A T A C G G T A G A
17 1-5 Chargaff's Ratio It has been found experimentally that the ratio of the amounts of adenine to thymine, and the ratio of guanine to cytosine, are always very close to unity for deoxyribose nucleic acid. Chargaff ratio A/T = G/C = 1
18 1-5 Chargaff's Ratio Homo Sapiens Drosophila melanogaster Zea mays Neurospora crassa Escherichia coli Bacillus subtilis Zamenhof, S., Brawerman, G., and Chargaff, E. Biochim. et Biophys. Acta 9, 402 (1952)
19 1-6 X-ray Crystallography The previously published X-ray data on <source> rg/stories/unravelling-thedouble-helix the deoxyribose nucleic acid are insufficient for a rigorous test of our structure. Rosalind Franklin ( )
20 1-7 Replication Mechanism It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material.
21 1-8 The Cathedral by Rodin Auguste Rodin ( ) The Cathedral
22 Review Watson met Crick at Cavendish. Together they did publish The double helical structure, Nature s masterly architecture. Bases complementary Are key to life s mystery Of heredity And 4 billion years prosperity.
23 Nobel Lectures in Science THANK YOU
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Hie-Joon Kim. Professor Emeritus Seoul National University. Experience. Representative Publications
Hie-Joon Kim Professor Emeritus Seoul National University B.S. Chemistry, Seoul National University, Korea, 1970 Ph.D. Chemistry, University of Chicago, USA, 1977 Experience Professor, Department of Chemistry
Hie-Joon Kim. Professor Emeritus Seoul National University. Experience. Representative Publications
Hie-Joon Kim Professor Emeritus Seoul National University B.S. Chemistry, Seoul National University, Korea, 1970 Ph.D. Chemistry, University of Chicago, USA, 1977 Experience Professor, Department of Chemistry
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Hie-Joon Kim. Professor Emeritus Seoul National University. Experience. Representative Publications
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