Dispensing with style

Transcription

1 Fast Efficient Ergonomic NEW! Repeater Xstream Repeater stream Dispensing with stye Manua and eectronic Repeater hand dispensers

2 The beauty of a sophisticated system Eectronic and manua Eppendorf Repeater dispensers and Combitips Pus The Instruments The manua Repeater Pus and the eectronic Repeater stream/xstream hand dispensers are idea for process acceeration and saving time on ong-series pipetting: their increased voume seection provides outstanding fexibiity and exceptiona support for research as we as routine tasks; and their smooth handing reduces the fatigue associated with repetitive work. The Consumabes Combitips Pus tips 1 easiy attach to the Repeaters; their buit-in sensor recognizes the size of the tip, and the voume appears automaticay in the dispay eiminating time-consuming voume cacuations and incorrect dispensing voumes. The nine sizes of Combitips Pus aow a wider dispensing range of up to 112 different voumes and onger series dispensing (e. g., 100 x 250 μ) without stopping for a refi. Aways contamination-free! A wide variety of soutions Combitips Pus tips function according to the positive dispacement principe, which ensures that the correct voume is dispensed regardess of the density or viscosity of the iquid. The positive dispacement system provides safer dispensing of soutions that contain radioactive and/or toxic substances as we as soutions that have a high vapor pressure because no aerosos are formed. Combitips are easiy repaced eject with a push of a button and disposed after use, aways contamination-free. The Concusion The Repeater/Combitips system is a sophisticated soution for repetitive iquid handing appications. These contamination-free manua and eectronic systems are designed with your productivity and safety in mind especiay when working with hazardous, voatie or viscous iquids. 2

3 A quantum eap in seria dispensing Repeater stream and Repeater Xstream eectronic hand dispensers Eectronic Repeater stream and Xstream combine easy handing with a broad range of features. When used with Combitips Pus tips, the dispenser/tip forms an unbeatabe system that is the quintessentia ab too for a appications: Repeater stream is suitabe for a standard pipetting and dispensing appications; for added versatiity, the Repeater Xstream can aso perform titrations, sequentia dispensing and aspiration of super natants. Appications Eectronic dispensing with programmabe memory functions Contamination-free dispensing using the positive dispacement principe Idea for viscous soutions (e.g., gycero, oi) or iquids with high vapor pressure (e.g., acetone, ethano) Dispensing in ong series Repeater stream and Repeater Xstream at a gance Feature Positive dispacement principe Rapid, ergonomic dispensing with motorized piston Automatic Combitips Pus tip recognition Two-year warranty IVD conformity One-button tip ejector Voume range from 1 μ to 50 m Li-ion battery Automatic dispensing Pipetting Dispensing Aspiration (pipetting of supernatants) Titration Sequentia dispensing 3

4 Reiabiity in your hand Repeater Pus manua hand dispenser Repeater Pus 2 is the idea precision instrument for reducing the time associated with manua, ong-series pipetting. Its increased voume seection provides outstanding fexibiity and a-around support for research and routine tasks. The eectronic dispay ensures error-free dispensing, and the rounded, grooved grip makes dispensing easier and ergonomic with ess stress on the hand and thumb musces. The rounded, grooved grip of the Repeater Pus means easier dispensing with ess stress on the hand and thumb musces Appications Dispensing voumes in ong series Contamination-free dispensing using the positive dispacement principe Idea for viscous soutions (e.g., gycero, oi) or iquids with high vapor pressure (e.g., acetone, ethano) Product features Easy to read: automatic Combitips recognition with eectronic voume dispay Easy voume setting Extremey fexibe with 112 different voumes from 1 μ to 10 m Up to 100 dispensings per fiing for easy processing of ong series 20 different dispensing voumes per each Combitips size Versatie, even for odd voumes (e.g., 1 μ, 3 μ or 17 μ) Safe, due to easy, contact-free Combitips attachment and ejection IVD conformity Two-year warranty 4

5 A pure peasure to work with Combitips Pus tips and accessories for Repeater dispensers Tip purity ensured In addition to standard purity, Combitips Pus tips are aso avaiabe in Sterie and Eppendorf Biopur. Eppendorf Biopur, signifying outstanding bioogica purity, guarantees that these tips are sterie as we as pyrogen-, RNase-, DNA- and ATP-free. They meet the most stringent demands of the medica, pharmaceutica and food industries, PCR appications and moecuar bioogy/ce technoogy abs. This purity is ensured through an automated manufacturing process, which safeguards the product against any contamination with bioogica substances. Individua ot certificates are avaiabe at Accessories As a safeguard against potentia contamination, our carouse stand does not hod the pipette at the bottom ike most other stands do. Eppendorf Repeater, Repeater Pus and Maxipettor pipettes easiy fit on the carouse with an adapter. To store the eectronic Repeater stream/xstream, a charging stand is avaiabe (sod separatey). Is your bench space at a premium? We aso offer the practica wa-mounted pipette bracket it keeps your Repeater pus off the bench but sti within reach. Product features of Combitips Pus Idea system component for a Repeater/Repeater Pus (manua), Repeater stream/xstream (eectronic) and EDOS (automatic dispensing) systems Higher throughput with up to 100 dispensings per fiing Reiabe resuts ensured by positive dispacement system Avaiabe in Eppendorf Quaity, Sterie and Eppendorf Biopur purity eves Avaiabe in 9 different sizes from 0.1 m to 50 m Certified trace meta reease Contamination-free use with Repeaters IVD conformity Wa-mounted pipette bracket Repeater stream/xstream charging stand Carouse stand 5 5

6 Precision and versatiity for professionas Appication exampes for the Repeater Pus Environmenta anaysis / Chemica aboratory Appication: Compete breakdown of a soi sampe, foowed by ICP determination of the main and trace eements K, Mg, Ca, Mn, Si, Fe, P, S, Pb, Cu, Zn, Cd Use: Addition of 2 m each of hydrofuoric acid (40%) and nitric acid (70%) to a microwave standard vesse HP 40; subsequent microwave treatment and addition of 2% nitric acid Dispenser: Repeater Pus with a 50 m Combitips Pus tip Location: Scheswig Hostein State Laboratory Diagnostics / Cinica aboratory Appication: Quantitative determination of Protein S (free and tota) by means of ELISA (Protein S pays a roe in the bood coaguation process) Use: Addition of 100 μ of stop soution to a microtest pate; this produces a change in coor Dispenser: Repeater Pus with 5 m Combitips Pus tip Location: Laboratory at Dr. v. Froreich and Partners Industria research / Immunoogy aboratory Appication: Phenyaanine screening assay (detection of L-pheny aanine in the bood spots of newborn babies) Use: Addition of 100 μ of stained trichoroacetic acid in connection with the further deveopment of the assay Dispenser: Repeater Pus with 5 m Combitips Pus tip Location: Scheswig Hostein State Laboratory Repeater References These we-known accounts are Repeater system customers: F. Hoffmann-La Roche Ltd. Bayer HeathCare AG Boehringer Ingeheim Pharma GmbH & Co. KG ALTANA Pharma AG ALTANA Chemie AG Liy Deutschand GmbH AstraZeneca GmbH 6

7 Dispensing acids, akais and organic sovents with the Repeater Pus A number of acids, akais and organic sovents with a variety of viscosities and densities have been tested in our aboratories with the Repeater/Combitips system. The resuts appear in the chart beow. Other industria appications The Repeater Pus is aso used in many other areas not directy associated with natura sciences. Its exceent abiity to dispense highy viscous iquids makes it idea for use in the automotive, petroeum and jewery industries, to name just a few. Varnishes, ois, even adhesives, can be dispensed with a great degree of accuracy and precision. Liquid Conc. 100 µ [d] 100 µ [cv] 1,000 µ [d] 1,000 µ [cv] Vapor pressure at 20 ºC [hpa] Density at 20 ºC [g/cm 3 ] Viscosity at 20 ºC [mpa s] Doube-distied water 100% ± ± Acetone 100% Acetonitrie 100% [25 ºC] Formid acid 98% Ammonium hydroxide 32% [26%] Aniine 100% Dimethy formamide (DMF) Dimethy sufoxide (DMSO) 100% % Ethy acetate 100% Ethano 98% Formadehyde 37% [25 ºC] Isopropano 99% Perchoric acid 60% Phosphoric acid 85% Nitric acid 65% / [40%] Hydrochoric acid 37% Sufuric acid 95 97% Tetrachoromethane 100% Tetrahydrofuran (THF) 100% Touene (methy benzene) 100% Trichoroethyene 100% Trichoromethane (choroform) 100% Triethyamine 100% Mean vaue Random [d] and systematic [cv] measurement deviations in percent when dispensing seected iquids The experiments were performed using a Repeater Pus with iquids at room temperature; the resuts of 10 dispensing operations with a 5 m Combitips Pus tip are shown (in chart above). 7

8 Combitips Pus tips for Repeater Pus and Repeater stream/xstream Technica specifications Combitips Pus Voume min./max. Increment/ min. step size Max. dispensing voume Voume Inaccuracy* Imprecision* for Repeater Pus 0.1 m 1 µ 1 µ 20 µ 2 µ ±1.6% 3.0% 20 µ 20 µ ±1.0% 2.0% 0.2 m 2 µ 2 µ 40 µ 4 µ ±1.3% 2.0% 40 µ 40 µ ±0.8% 1.5% 0.5 m 5 µ 5 µ 100 µ 10 µ ±0.9% 1.5% 100 µ 100 µ ±0.8% 0.6% 1 m 10 µ 10 µ 200 µ 20 µ ±0.9% 0.9% 200 µ 200 µ ±0.6% 0.4% 2.5 m 25 µ 25 µ 500 µ 50 µ ±0.8% 0.8% 500 µ 500 µ ±0.5% 0.3% 5 m 50 µ 50 µ 1,000 µ 100 µ ±0.6% 0.6% 1,000 µ 1,000 µ ±0.5% 0.25% 10 m 100 µ 100 µ 2,000 µ 200 µ ±0.5% 0.6% 2,000 µ 2,000 µ ±0.5% 0.25% 25 m 250 µ 250 µ 5,000 µ 500 µ ±0.4% 0.6% 5,000 µ 5,000 µ ±0.3% 0.25% 50 m 500 µ 500 µ 10,000 µ 1,000 µ ±0.3% 0.5% 10,000 µ 10,000 µ ±0.3% 0.25% for Repeater stream and Xstream 0.1 m 1 µ 0.1 µ 0.1 m 10 µ ±1.6% 2.5% 100 µ 100 µ ±1.0% 0.5% 0.2 m 2 µ 0.2 µ 0.2 m 20 µ ±1.3% 1.5% 200 µ 200 µ ±1.0% 0.5% 0.5 m 5 µ 0.5 µ 0.5 m 50 µ ±0.9% 0.8% 500 µ 500 µ ±0.9% 0.3% 1 m 10 µ 1 µ 1 m 100 µ ±0.9% 0.55% 1 m 1,000 µ ±0.6% 0.2% 2.5 m 25 µ 2.5 µ 2.5 m 250 µ ±0.8% 0.45% 2.5 m 2,500 µ ±0.5% 0.15% 5 m 50 µ 5 µ 5 m 500 µ ±0.8% 0.35% 5 m 5,000 µ ±0.5% 0.15% 10 m 100 µ 10 µ 10 m 1,000 µ ±0.5% 0.25% 10 m 10,000 µ ±0.4% 0.15% 25 m 250 µ 25 µ 25 m 2,500 µ ±0.3% 0.25% 25 m 25,000 µ ±0.3% 0.15% 50 m 500 µ 50 µ 50 m 5,000 µ ±0.3% 0.25% 50 m 50,000 µ ±0.3% 0.15% *The data for imprecision (random measurement error) and inaccuracy (systematic measurement error) according to EN ISO 8655 is vaid ony when origina Eppendorf Combitips Pus tips are used. 8

9 Voume tabe for using Combitips Pus tips with the Repeater Pus (The positions and dispensing steps that are marked in bod are based on Combitips Pus used with the origina Eppendorf Repeater) Position of the voume seection dia: 0.5 1/ / / / / Maximum number of dispensing voumes: / / / / / Combitips Pus with the foowing voumes: 0.1 m 1 μ 2 μ 3 μ 4 μ 5 μ 6 μ 7 μ 8 μ 9 μ 10 μ 11 μ 12 μ 13 μ 14 μ 15 μ 16 μ 17 μ 18 μ 19 μ 20 μ 0.2 m 2 μ 4 μ 6 μ 8 μ 10 μ 12 μ 14 μ 16 μ 18 μ 20 μ 22 μ 24 μ 26 μ 28 μ 30 μ 32 μ 34 μ 36 μ 38 μ 40 μ 0.5 m 5 μ 10 μ 15 μ 20 μ 25 μ 30 μ 35 μ 40 μ 45 μ 50 μ 55 μ 60 μ 65 μ 70 μ 75 μ 80 μ 85 μ 90 μ 95 μ 100 μ 1.0 m 10 μ 20 µ 30 μ 40 μ 50 μ 60 μ 70 μ 80 μ 90 μ 100 μ 110 μ 120 μ 130 μ 140 μ 150 μ 160 μ 170 μ 180 μ 190 μ 200 μ 2.5 m 25 μ 50 μ 75 μ 100 μ 125 μ 150 μ 175 μ 200 μ 225 μ 250 μ 275 μ 300 μ 325 μ 350 μ 375 μ 400 μ 425 μ 450 μ 475 μ 500 μ 5 m 50 μ 100 μ 150 μ 200 μ 250 μ 300 μ 350 μ 400 μ 450 μ 500 μ 550 μ 600 μ 650 μ 700 μ 750 μ 800 μ 850 μ 900 μ 950 μ 1.0 m 10 m 100 μ 200 μ 300 μ 400 μ 500 μ 600 μ 700 μ 800 μ 900 μ 1.0 m 1.1 m 1.2 m 1.3 m 1.4 m 1.5 m 1.6 m 1.7 m 1.8 m 1.9 m 2.0 m 25 m* 250 μ 500 μ 750 μ 1.0 m 1.25 m 1.5 m 1.75 m 2.0 m 2.25 m 2.5 m 2.75 m 3.0 m 3.25 m 3.5 m 3.75 m 4.0 m 4.25 m 4.5 m 4.75 m 5.0 m 50 m* 500 μ 1.0 m 1.5 m 2.0 m 2.5 m 3.0 m 3.5 m 4.0 m 4.5 m 5.0 m 5.5 m 6.0 m 6.5 m 7.0 m 7.5 m 8.0 m 8.5 m 9.0 m 9.5 m 10 m *25 m and 50 m Combitips Pus must be used with the suppied adapter. Repeater pipettes, Combitips Pus tips and accessories Ordering information Description Cataog No. Repeater Pus Repeater stream, incudes charging adapter, V/50 60 Hz Repeater Xstream, incudes charging adapter, V/50 60 Hz Charging stand, for Repeater stream/xstream, hods one unit Stand for Repeater pipettes, hods two units Repeater Pus adapter, for new pipette carouse stand Repeater Pus Wa Mount Pipette Carouse Stand, for six pipettes Combitips Rack, for mounting Combitips Pus tips Combitips Pus tips Cataog No. Standard (set of 100) Cataog No. Sterie (10 bags of 10 tips = 100 tips) Cataog No. Eppendorf Biopur** (individuay wrapped, set of 100) 0.1 m m m m m m m m m m adapter (1 piece), bue m adapter (1 piece), dark gray Other 25 m adapter industria (set of appications 7) The 50 m Repeater adapter (set is aso of 7) used in many other areas not industries, to name just a few. Varnishes, ois or even directy Combitips associated Pus tips assortment with natura pack, sciences. Its exceent adhesives, for exampe can be dispensed with this capabiity incudes five of of dispensing each size pus highy one viscous iquids make it universa manua dispenser with a great degree of 50 m and one 25 m adapter idea for use in the automotive, petroeum and jewery accuracy and precision. Combitube (set of two), aspirating aid for removing iquids from voumetric fasks and ta bottes; Combitips Pus tips sizes 5 m, 25 m and 50 m **Eppendorf Biopur Combitips (individuay wrapped) are guaranteed to be sterie, RNase-, DNA-, ATP- and pyrogen-free. 9 9

10 Changing the face of pipetting: Eppendorf s 8 PhysioCare Concept Criteria: Integrated Systems Exceptiona durabiity Optimized form and function Perfecty baanced Intuitive operation Minima user exertion Simpe maintenance Tota support Visit the specia Eppendorf PhysioCare Concept Website Check out onine how good your pipette reay is! Read more about the PhysioCare Concept! Get vauabe tips and tricks on pipetting! Connect With Us