SUREPETTE USER'S GUIDE. AccuBioTech Co., Ltd. Adjustable Volume Pipette Fixed Volume Pipette. Accurate, Reliable, Cost Effective

Transcription

1 SUREPETTE USER'S GUIDE Adjustabe Voume Pipette SUR EPE TTE Fixed Voume Pipette AccuBioTech Co., td. Accurate, Reiabe, Cost Effective

2

3 CONTENTS PAGE. INTRODUCTION 2. PARTS CHECK IST 3. DESCRIPTION 2 4. OPERATING RANGES 2 5. SETTING THE VOUME 3 6. PIPETTING 4 7. GUIDEINES FOR GOOD PIPETTING 6 8. RECAIBRATION 7 9. MAINTENANCE 8 0. TROUBE SHOOTING 9. STORAGE 2. SPECIFICATIONS

4 . INTRODUCTION Thanks for your trust in our SUREPETTE Series Pipettes. et's share the foowing benefits from SUREPETTE : ). SUREPETTE Series Pipettes incude Adjustabe Voume Pipettes and Fixed Voume Pipettes, covering a voume range from 0.5 to 5 m; 2). SUREPETTE Series Pipettes aspirate and dispense voumes of iquid precisey and safey; 3). A voumeter makes it easy to set voume and to eiminate cacuation errors; 4). A tip ejector button simpifies the discarding of the tip and makes the operation safe. 5). Each SUREPETTE is factory caibrated and carefuy checked gravimetricay using distied water and an anaytica baance. 2. PARTS CHECK IST Our SUREPETTE package contains the foowing items: - Pipette - Service Too - Grease - User's Guide - Pipette Hoder - Tip Pease take a moment to verify that the above items are present.

5 3. DESCRIPTION Push Button SUREPETTE Push Button Rod Tip Ejector Button Hande Voumeter Tip Ejector Coar Tip Hoder Tip 4. OPERATING RANGES Adjustabe Voume Pipettes: Mode Range

6 SPA-000 SPA M Fixed Voume Pipette Mode Voume SPF-5 5 SPF-0 0 SPF SPF SPF SPF SPF SPF SPF SPF SPF SPF SETTING THE VOUME The voume of SUREPETTE is ceary shown through the voumeter. For the Adjustabe Voume Pipettes, the deivery voume is set by turning the push button cockwise or anticockwise unti the desired deivery voume cicks into pace and the digits are competey visibe in the voumeter; WARNING: AWAYS adjust the voume within the designated voume range. Do not turn the push button with excessive force. This prevents the pipettes from inaccuracy and damage. 3

7 Exampe for each pipette: SPA-0 (Adjustabe Unit Voume= ) SPA-20 (Adjustabe Unit Voume= ) ) SPA-50 (Adjustabe Unit Voume= SPA-00 (Adjustabe Unit Voume= 0 0 SPA-200 ) (Adjustabe Unit Voume= ) ) SPA-000 (Adjustabe Unit Voume= SPA-5000 (Adjustabe Unit Voume= ) PIPETTING ). Attach a new disposabe tip to the tip hoder. Press ony enough to make a positive airtight sea. This avoids eaks or poor accuracy; 4

8 2). Pre-rinse the tip by aspirating the first voume of iquid and then dispensing it back or to waste; 3). Press the push button to the first stop. This part of the stroke is the voume dispayed on the voumeter; 4). Hoding SUREPETTE verticay, immerse the tip into the iquid to a depth of 2-4 mm; 5). Wait 2-3 seconds. Aow the push button to return sowy and smoothy to the top position. DO NOT et it snap up; 6). Withdraw the tip from the iquid after the fu voume of sampe is drawn into the tip, touching against the edge of the container to remove excess; 7). To dispense sampe, pace the tip end against the inside wa of the recipient vesse at an ange of 0 to 40. Depress the push button sowy and smoothy to the first stop. Wait - 3 seconds. Then press the push button to the second stop (bottom of stroke), expeing the residua iquid from the tip. 8). Withdraw SUREPETTE from the vesse carefuy. Aow the push button to return to the top position. 9). Discard the tip by depressing the tip ejector button. 5

9 Top st stop 2nd stop 7. GUIDEINES FOR GOOD PIPETTING ). Aways contro the push button movements with the thumb sowy and smoothy. 2). Hod the pipette verticay when aspirating the iquid and pace the tip ony a few miimeters into the iquid. 3). Change the tip before aspirating a different iquid, sampe, or reagent. 4). Change the tip if there is residua iquid at the end of tip. 5). When using new tips, pre-rinse the tips with the iquid to be pipetted severa times to eiminate the chances of measuring error. 6

10 6). iquid shoud never enter the tip hoder; to prevent this: - press and reease the push button sowy and smoothy; - never turn the pipette upside down; - never ay the pipette on its side when there is iquid in the tip. 7). When pipetting iquids with temperatures different from the ambient temperature, pre-rinse the tip severa times before use. 8). Do not pipette iquids which have extreme temperatures ( 70, or 4 ). 8. RECAIBRATION SurePettes are factory caibrated and carefuy checked gravimetricay before shipment using distied water, an anaytica baance (accuracy is / 0,000 to /00,000) and a beaker. Normay, the recaibration is not required; but, if necessary, the caibration can be taken easiy in your abs, foowing the beow steps: ). Pace the service too into the hoes of the caibration adjustment ock (under the push button). 2). Turn the adjustment ock anticockwise to decrease and cockwise to increase the voume. 7

11 3). Pipette the distied water into the beaker foowing the pipetting procedures and measure with an anaytica baance. Repeat the measurement six times. The resuts shoud be consistent with the specifications. Otherwise, caibrate and test again unti the resuts are consistent with the specifications. 9. MAINTENANCE Ceaning Use ethano and a soft coth or int-free tissue to cean your SurePette. It is recommended to cean the tip hoder reguary. Maintenance ). Depress the tip ejector button. 2). Pace the tooth of the service too between the tip ejector and the tip ejector coar to reease the ocking mechanism. 3). Carefuy reease the tip ejector and remove the tip ejector coar. 4). Pace the wrench end of the service too over the tip hoder, turning it anticockwise. Do not use any other toos. The 5m tip hoder is removed by turning it anticockwise. Do not use any toos. 8

12 5). Wipe the piston, the O-ring and the tip hoder with ethano and a int-free coth. Note: Some modes have a fixed O-ring ocated inside the tip hoder. In this case, the Oring can not be accessed for maintenance. 6). Before repacing the tip hoder, it is recommended to grease the piston sighty using the grease provided. Note: Excessive use of grease may jam the piston. 7). After reassembing, use the pipette without iquid severa times to make sure that the grease is spread eveny. 8). Check the performance and recaibrate the pipette. 0. TROUBE SHOOTING A quick inspection of the pipette referring to the beow questions may hep you isoate the probem. - Is the push button rod straight? - Is the push button free of chemica damage? - Are the voumeter digits cear and easy to read? - Can you set any voume in the usefu range? - Can you set the maximum voume? - Does the voumeter operate smoothy without hitching? If the answer to any of these questions is NO, the pipette probaby has been damaged as the resut of a mechanica shock or chemica damage, in which case it shoud be returned to your suppier 9

13 for repair. If the answer to these questions is YES, continue as foows: - Is the tip ejector straight? - Is the tip ejector free of chemica damage? - Remove tip ejector; is the tip hoder undamaged and free of chemica damage? - Are the O-ring and sea undamaged and free of chemica damage? If the answer is NO, cean or repace the damaged part. If the answer is YES, you may be abe to identify and to correct the probem by reference to the beow tabe. If you can not sove the probem, contact your SurePette representative. T roube Dropets eft ins ide the tip eakage or pipetted voume too s ma P os s ibe C aus e Uns uitabe tip T ip incorrecty attached Uns uitabe tip P artices between tip and tip hoder Ins ufficient amount of greas e on pis ton O - ring not correcty pos itioned or damaged Incorrect operation C aibration atered P us h button jammed or P is ton contaminated moves erractica P enetration of s ovent vapours P ipette bocked, iquid has penetrated as pirated voume too tip hoder and dried s ma T ip ejector jammed or T ip hoder and/or tip moves erracticay ejector coar contaminated 0 S oution Us e origina T ips Attach firmy Us e origina T ips C ean the tip hoder, attach new tip C ean and greas e the pis ton C hange the O - ring F oow the ins truction carefuy R ecaibrate C ean and greas e O - ring and pis ton. C ean the tip hoder C ean and greas e O - ring and pis ton. C ean the tip hoder C ean the tip hoder and the tip ejector coar

14 . STORAGE For convenience and safety, aways keep your SurePette verticay on its own hoder provided when not in use. When instaing the hoder, pease foow the beow instructions: ). Cean the shef surface with ethano; 2). Remove the protective paper from the adhesive tape of the hoder; 3). Press the adhesive side of the hoder against the edge of the shef; We aso provide SurePette Stand to hod 5 SurePettes. 2. SPECIFICATIONS Fixed Voume Pipettes Norma Accuracy P recis ion V oume (mean error) (repeatabiity) % % S PF S P F S P F S P F S P F S P F S P F S P F S P F S P F S P F S P F Mode

15 Adjustabe Voume Pipettes Norma Accuracy P recis ion Mode & V oume (mean error) (repeatabiity) V oume R ang % % S P A ~0 * 5 0 ± 2 ± 6. 4 ± S P A ~ ± 0 ± 3. 2 ± S P A ~ ± 6. 4 ± 3. 0 ± S P A ~ ± 3. 2 ± 2. 4 ± S P A ~ ± 3. 0 ±. 5 ± S P A ~ ±. 5 ±. 5 ± ±. 5 ±. 0 ± S P A ~5000 * Ony for the practiced. 2

16 AccuBioTech Co., td. Accurate, Reiabe, Cost Effective