Microbial DNA qpcr Array Sepsis
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1 Microbial DNA qpcr Array Sepsis Cat. no BAID-1903ZRA For real-time PCR-based, application-specific microbial identification or profiling The Sepsis Microbial DNA qpcr Array is a research tool used to screen for pathogenic bacteria and fungi associated with bloodstream infections. Sepsis results from the body s severe response to microorganisms present in the bloodstream and other body sites. This array provides microbiology researchers with a convenient way to quickly detect the presence of pathogenic microorganisms from sepsis-associated samples such as blood, blood culture, or isolated bacterial colonies, and can be used to monitor the frequency of sepsis-related microbial infections in epidemiology research studies. The array contains assays for 89 gram-positive and gram-negative bacterial pathogens as well as fungal pathogenic species from the Aspergillus and Candida genera. The array also contains assays for methicillin-resistant aureus. Assays were designed to detect bacterial 16S rrna gene and fungal ribosomal rrna gene sequences. For detection of methicillin-resistant aureus, assays were designed to detect the antibiotic resistance gene, meca, and the virulence factor genes, lukf and spa. These assays use PCR amplification primers and hydrolysis-probe detection, which increases their specificity. Pan-bacteria assays that detect a broad range of bacterial species are included to serve as positive controls for the presence of bacterial DNA, and the Positive PCR Control assay is included to test for the presence of PCR inhibitors and the efficiency of the polymerase chain reaction. The arrays also include the appropriate Microbial qpcr Mastermix and Microbial DNA-Free Water. Identification of which microbial pathogens are present in a sample is accomplished using data analysis software with imported CT values generated by the PCR instrument software. The simplicity of the product format and operating procedure allow routine and reliable screening of microbial pathogens from samples in any research laboratory with access to a real-time PCR instrument. Sample & Assay Technologies
2 Format Format A, with fluorescein For use with the following real-time cyclers Bio-Rad models icycler, iq 5, MyiQ, MyiQ2 Format A, with ROX Applied Biosystems models 5700, 7000, 7300, 7500, 7700, 7900HT, ViiA 7 (96-well blocks); Bio-Rad/MJ Research Chromo4 ; Eppendorf Mastercycler ep realplex models 2, 2s, 4, 4s; Stratagene models Mx3005P, Mx3000P Format C, with ROX Format D, with ROX Applied Biosystems models 7500 (Fast, 96-well block), 7900HT (Fast, 96-well block), StepOnePlus, ViiA 7 (Fast, 96-well block) Bio-Rad CFX96 ; Bio-Rad/MJ Research models DNA Engine Opticon, DNA Engine Opticon 2; Stratagene Mx4000 Format E, with ROX Applied Biosystems models 7900HT (384-well block), ViiA 7 (384-well block); Bio-Rad CFX384 Format F, with ROX Format G, with ROX Roche LightCycler 480 (96-well block) Roche LightCycler 480 (384-well block) Shipping and storage Microbial DNA qpcr Arrays are shipped at ambient temperature or on blue ice packs. For long-term storage, keep plates at 20 C. Ensure that you have the correct Microbial DNA qpcr Array format for your real-time cycler (see table above). Microbial qpcr Mastermixes are shipped on blue ice packs. For long-term storage, keep Microbial qpcr Mastermixes at 20 C. Microbial DNA-Free Water is shipped at ambient temperature or on blue ice packs. If unopened, Microbial DNA-Free Water can be stored at room temperature or at 20 C. If tube is opened, store Microbial DNA-Free Water at 20 C. Discard tube of Microbial DNA-Free Water if opened three times and use fresh tube of Microbial DNA-Free Water for future experiments. Note: Ensure that you have the correct Microbial qpcr Mastermixes, with the correct reference dye if required, for your instrument. Note: Open the package and store the products appropriately immediately upon receipt.
3 Assay Table Position Species (NCBI Tax ID)/Gene NCBI Tax ID Antibiotic classification / Gene Description May detect (species) / Also detect (antibiotic resistance genes) / Associated species (virulence factor genes) Sensitivity Assay Catalog # A01 Achromobacter xylosoxidans BPID00002A A02 Acinetobacter baumannii BPID00004A A03 Acinetobacter rhizosphaerae(243922) Acinetobacter BPID00005A calcoaceticus A04 Aerococcus viridans BPID00021A A05 Aeromonas enteropelogenes(29489), Aeromonas punctata(648), 644 Shewanella benthica(43661) 100 BPID00022A Aeromonas media(651) Aeromonas hydrophila A06 Aeromonas veronii(654) Aeromonas sobria BPID00023A A07 Alcaligenes faecalis BPID00027A A08 Aspergillus flavus BPID00037A A09 Aspergillus fumigatus BPID00038A A10 Bacillus anthracis 1392 Bacillus weihenstephanensis(86662), Bacillus cereus(1396) 40 BPID00042A A11 Bacillus anthracis(1392) Bacillus cereus 1396 Bacillus cytotoxicus(580165), Bacillus weihenstephanensis(86662) 40 BPID00043A A12 Bacillus sonorensis(119858) BPID00044A Bacillus licheniformis B01 Bacteroides fragilis BPID00051A B02 Bifidobacterium longum BPID00067A B03 Brevibacterium casei BPID00074A B04 Brevundimonas diminuta BPID00075A B05 Brevundimonas vesicularis Brevundimonas diminuta(293) 20 BPID00076A B06 Burkholderia vietnamiensis(60552), Burkholderia pyrrocinia(60550), 292 Burkholderia gladioli(28095) 100 BPID00077A Burkholderia cenocepacia(95486) Burkholderia cepacia B07 Burkholderia pseudomallei(28450) BPID00079A Burkholderia mallei B08 Butyrivibrio fibrisolvens BPID00082A B09 Candida albicans BPID00092A B10 Candida glabrata BPID00093A B11 Candida krusei BPID00094A B12 Candida parapsilosis BPID00095A C01 Candida tropicalis BPID00096A C02 Citrobacter freundii 546 Dickeya dadantii(204038), Klebsiella oxytoca(571), 100 BPID00108A Serratia marcescens(615) C03 Clostridium perfringens BPID00112A C04 Clostridium sordellii BPID00114A C05 C06 Corynebacterium diphtheriae Desulfovibrio desulfuricans 1717 Corynebacterium simulans(146827) 30 BPID00122A BPID00127A C07 Klebsiella oxytoca(571) Enterobacter cloacae 550 Citrobacter farmeri(67824), Enterobacter hormaechei(158836), Klebsiella granulomatis(39824), Klebsiella variicola(244366), Enterobacter aerogenes(548), 100 BPID00139A
4 Position Species (NCBI Tax ID)/Gene NCBI Tax ID Antibiotic classification / Gene Description May detect (species) / Also detect (antibiotic resistance genes) / Associated species (virulence factor genes) Sensitivity Assay Catalog # Klebsiella pneumoniae(573), Serratia marcescens(615) C08 Enterococcus faecalis BPID00142A C09 Enterococcus faecium 1352 Enterococcus avium(33945), Enterococcus durans(53345), Enterococcus hirae(1354) 40 BPID00143A C10 Erysipelothrix rhusiopathiae BPID00145A C11 C12 D01 D02 D03 Escherichia fergusonii(564),shigella boydii(621),shigella sonnei(624),shigella dysenteriae(622), Shigella flexneri(623) Escherichia coli Exiguobacterium aurantiacum Fusobacterium mortiferum Fusobacterium necrophorum Fusobacterium nucleatum 623 Escherichia albertii(208962), Enterobacter aerogenes(548), Enterobacter cloacae(550), Serratia marcescens(615) 30 BPID00146A BPID00153A BPID00158A BPID00159A BPID00160A D04 Fusobacterium varium BPID00162A D05 Geobacillus stearothermophilus BPID00167A D06 Haemophilus influenzae 727 Haemophilus haemolyticus(726) 20 BPID00171A D07 Hafnia alvei 569 Pectobacterium atrosepticum(29471), Pectobacterium 20 BPID00173A wasabiae(55208),serratia proteamaculans(28151) D08 Helicobacter pylori 210 Helicobacter suis(104628) 50 BPID00176A D09 Kocuria kristinae BPID00182A D10 Leifsonia aquatica BPID00202A D11 Methylobacterium fujisawaense BPID00213A D12 Methylobacterium zatmanii BPID00215A E01 Micrococcus luteus BPID00217A E02 Morganella morganii 582 Escherichia albertii(208962), Providencia 100 BPID00224A alcalifaciens(126385) E03 Neisseria meningitidis 487 Neisseria cinerea(483), Neisseria gonorrhoeae(485) 300 BPID00241A E04 Nocardia farcinica BPID00247A E05 Ochrobactrum tritici(94626) BPID00250A Ochrobactrum anthropi E06 Paenibacillus larvae BPID00252A E07 Paenibacillus macerans BPID00253A E08 E09 Paenibacillus thiaminolyticus Pantoea ananatis(553) Pantoea agglomerans Paenibacillus popilliae(78057) Erwinia tasmaniensis(338565), Kluyvera ascorbata(51288), Sodalis glossinidius(63612) 20 BPID00254A 30 BPID00255A E10 Pediococcus acidilactici BPID00262A E11 Pediococcus pentosaceus 1255 Pediococcus acidilactici(1254) 20 BPID00263A E12 Plesiomonas shigelloides BPID00267A F01 Prevotella bivia BPID00272A F02 Prevotella intermedia BPID00277A F03 Prevotella melaninogenica BPID00279A F04 Propionibacterium acnes BPID00285A Proteus vulgaris(585) Candidatus
5 Position Species (NCBI Tax ID)/Gene NCBI Tax ID Antibiotic classification / Gene Description May detect (species) / Also detect (antibiotic resistance genes) / Associated species (virulence factor genes) Sensitivity Assay Catalog # F05 Proteus mirabilis 584 hamiltonella(568987) 200 BPID00287A F06 Pseudomonas aeruginosa BPID00288A F07 Ewingella americana(41202) BPID00293A Rahnella aquatilis F08 Ralstonia pickettii BPID00294A F09 epidermidis 1282 aureus(1280), haemolyticus(1283), pettenkoferi(170573) 100 BPID00316A F10 arlettae(29378) BPID00317A saprophyticus F11 Xanthomonas retroflexus(305959), Pseudomonas geniculata(86188) BPID00318A Stenotrophomonas maltophilia F12 agalactiae BPID00320A G01 anginosus BPID00321A G02 mitis infantis(68892), oralis(1303), pneumoniae(1313), porcinus(1340), pseudopneumoniae(257758) 100 BPID00327A G03 mutans BPID00328A G04 G05 pneumoniae(1313), infantis(68892) oralis pneumoniae equi(1336), pseudopneumoniae(257758), mitis(28037) infantis(68892), oralis(1303), pseudopneumoniae(257758), mitis(28037) 40 BPID00329A 20 BPID00331A G06 pyogenes BPID00332A G07 thermophilus(1308) salivarius BPID00333A G08 sanguinis 1305 pseudopneumoniae(257758) 50 BPID00334A G09 bikiniensis BPID00337A G10 mediolani(68237), microflavus(1919), anulatus(1892), BPID00338A parvus(66428), finlayi(67296) griseus G11 Vibrio cholerae BPID00353A G12 Vibrio parahaemolyticus 670 Vibrio harveyi(669),vibrio orientalis(28175),vibrio shilonii(62153) 40 BPID00354A H01 Vibrio vulnificus BPID00355A H02 Weissella confusa BPID00356A H03 Yersinia enterocolitica BPID00357A Yersinia
6 Position Species (NCBI Tax ID)/Gene NCBI Tax ID Antibiotic classification / Gene Description May detect (species) / Also detect (antibiotic resistance genes) / Associated species (virulence factor genes) Sensitivity Assay Catalog # H04 pseudotuberculosis(633) 633 Yersinia rohdei(29485) 100 BPID00358A Yersinia pestis H05 aureus 1280 epidermidis(1282) 100 BPID00314A H06 meca Beta-lactam resistance 40 BPAR00374A H07 lukf Panton-Valentine leukocidin chain F aureus 20 BPVF00517A precursor H08 spa Immunoglobulin G binding protein A aureus 200 BPVF00518A precursor H09 Pan Aspergillus/Candida 5052 BPCL00359A H10 Pan Bacteria 1 BPCL00360A H11 Pan Bacteria 3 BPCL00362A H12 PPC BPCL00365A No Template Control (NTC)>35 Microbial DNA Positive Control<34 Note: On Stratagene instruments, Ct of the positive control template >34
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8 Ordering Information Product Contents Cat. no. Microbial DNA qpcr Array Related Products Array plate, master mix, and microbial DNA-free water for detection of microbial species or genes Supplemental Microbial qpcr Mastermix ROX Supplemental Microbial qpcr Mastermix Fluor 2 tubes of 1.35 ml each tubes of 1.35 ml each Microbial DNA-Free Water 12 tubes of 1.35 ml each Microbial DNA qpcr Arrays are intended for molecular biology use only. These products are not intended for the diagnosis, prevention, or treatment of a disease.. Visit today to view the full array list! Trademarks: QIAGEN ; Bio-Rad, CFX96, CFX384, Chromo4, icycler, IQ, Opticon (Bio-Rad Laboratories, Inc.); Eppendorf, Mastercycler (Eppendorf AG); Applied Biosystems, ROX, StepOnePlus, ViiA, (Life Technologies Corp.); Roche, LightCycler (Roche Group); Stratagene, Mx3005P, Mx3000P, Mx4000 (Stratagene) / QIAGEN, all rights reserved. Canada Ireland Norway China Italy Singapore Denmark Japan Spain Australia Finland Korea (South) Sweden Austria 0800/ France Luxembourg Switzerland Belgium Germany Mexico UK Brazil Hong Kong The Netherlands USA Sample & Assay Technologies
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