Rapid Test Methods. Bacteria Presumptive ID Additional Tests for Definitive ID

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1 Latest Update... Rapid Tests from Hardy Diagnostics which comply with the latest update of CLSI s Standards M35-A2 for the Rapid ID of Bacteria and Yeast.

2 Rapid Test Methods Bacteria Presumptive ID Additional Tests for Definitive ID Organism Test Catalog No. Notes Brucella PDA negative (Cat. no. Z7331, K530, L21, Z63) Forward to LRN reference laboratory Work in a safety cabinet; highly infectious MacConkey Urea L65 Campylobacter jejuni/coli Cardiobacterium hominis Eikenella corrodens Motility Hippurate hydrolysis Indoxyl acetate MacConkey Colonial morphology on Blood or chocolate in CO 2 MacConkey Hemolytic reaction Distinct odor of bleach DH4525, Z52 Z111 Z23, E14 Must be isolated from stool at 42 C on Campy selective medium Isolate must be from blood culture Confirm with negative nitrate test (Cat. no. K42, Z71, Z72, Z73, Z7071) Rapid Ornithine (Cat. no. K279) Indoxyl Acetate Disks for Campylobater jejuni (Cat. no. Z111), showing positive (left disk) and negative (right disk) reactions. PYR Test Kit (Cat. no. Z75 and Z175) for Group A Strep or Enterococcus. Readout in only two minutes! CatScreen (Cat. no. Z110) for Moraxella catarrhalis. Detects butyrase activity, readout in only five minutes!

3 Organism Test Catalog No. Notes Escherichia coli Isolate must be growing as large colonies and not from gastrointestinal sites Gram reaction or growth on gram-negative selective agar ; or Lactose, PYR Z75, Z175, Z104 MUG K9101 Francisella Work in a safety cabinet; highly infectious tularensis Forward to LRN reference laboratory Growth characteristics on chocolate or around E14 Staphylococcus streak Beta lactamase Z51 Haemophilus influenzae Time for growth on Chocolate/BAP, or MacConkey agar E14, Growth on BAP only around staph separates from Brucella and Francisella if necessary ALA Z7081 Kingella kingae Found in sterile tissues and fluids Moraxella catarrhalis Neisseria meningitidis MacConkey Entire non-hemolytic colony moves intact on BAP when pushed Butyrate; or Indoxyl acetate Colonial morphology on Chocolate in CO 2 g-glutamyl-aminopeptidase Z110 Z111 E14 K9187 Work in safety cabinet; highly infectious OxiStrip (Cat. no. Z93) An oxidase test, paper strip with a stabilized sensitive reagent. See also our OxiDrops (Cat. no. ) for a liquid ready-to-use reagent in dropper bottle. M.O.T. Disks (Cat. no. K9101) showing positive MUG reaction (left) E. coli and negative MUG reaction (right) Proteus mirabilis. Performs a MUG, ONPG, and test. ALA Differentiation Disks for Haemophilus (Cat. no. Z7081) showing positive (left disk) and negative (right disk) reactions under UV light.

4 Bacteria Presumptive ID Additional Test for Definitive ID Organism Test Catalog No. Notes Neisseria lactamica Colonial morphology on BAP b-galactosidase K9187 Neisseria gonorrhoeae Z262 g-glutamyl-aminopeptidase; and K9187 b-galactosidase Proteus mirablis Spreading colony Ornithine (Cat. no. K279 ) or Maltose (Cat. no. Y85) Proteus vulgaris Pseudomonas aeruginosa Staphylococcus aureus Staphylococcus lugdunensis No other testing, if ampicillin susceptible Spreading colony on BA (Cat. no. ) Odor Tube coagulase required if not hemolytic Tube or slide coagulase (Rabbit plasma w/edta) or latex agglutination Colonial morphology Z202 ST50 or ST200 May be slide coagulase positive or clumpy (Cat. no. ST50, ST200) Tube coagulase (Rabbit plasma w/edta) Z202 Enterococcus spp. Aerococcus viridans PYR and Z75, Z175 Polymixin B Rapid Ornithine K279 LAP positive if alpha hemolytic (Cat. no. Z122) Hemolytic colony size on BAP PYR Z75, Z175 PYR (Cat. no. Z122) LAP (Cat. no. Z122) Spot Test (Cat. no. ) showing positive indole reaction. Filter paper was saturated with Spot reagent and E. coli colonies were applied subsequently. Rapid Ornithine media (Cat. no. K279) detects ornithine decarboxylase activity in as little as four hours. Reaction to the right shows a positive results for Staphylococcus lugdunensis. Test (Cat. no. Z62) showing a positive catalase reaction. Growth was from a 24 hour culture of Staphylococcus aureus.

5 StrepQuick (Cat. no. Z122) a rapid test for the identification of gram-positive, catalase-negative cocci. Helpful for ID of Streps, Aerococcus, Enterococcus, Gemella, Leuconostoc, and Pediococcus. StrepPRO Grouping Kit (Cat. no. PL030HD) a latex agglutination kit for beta hemolytic streptococcus (A, B, C, D, F, and G). Bile Spot Reagent (Cat. no. Z61) pneumoniae colony after incubation with Bile Spot Reagent. Reagent was dropped within the black circle. Incubated aerobically for 30 minutes at 35 o C to dissolve colonies. Organism Test Catalog No. Notes Listeria Isolate should be from either blood culture monocytogenes or CSF agalactiae (Group B) anginosus group ( S. milleri ) pneumoniae on BAP Motility Hippurate on BAP Hippurate; or Rapid CAMP; or Lancefield Group B by latex agglutination Colony size and hemolysis on BAP Odor, or Lancefield group F by latex agglutination on BAP DH4525 or Z52 DH4525 or Z52 Z305 and Z306 PL030HD PL030HD In nonhemolytic, do not use hippurate method May be Lancefield group A, C, F, or G by latex agglutination (Cat. no. PL030HD) Some strains have lost solubility to bile. Bile solubility Z61 pyogenes (Group A) on BAP Carefully look at size and hemolysis since enterococci can also be hemolytic Colony size PYR positive; or Lancefield group A by latex agglutination Z75, Z175 PL030HD viridans group If not in chains-pediococcus is vancomycin resistant and Aerococcus urinae is a urinary pathogen in tetrads. PYR Z75, Z175 LAP Z122 Bile solubility if alpha hemolytic Z61

6 Yeast Organism Test Catalog No. Notes Candida albicans Budding yeast in smear Cannot separate from C. dubliniensis Feet in less than 48 hours Candida glabrata Cryptococcus neoformans Germ tube positive Small yeast in smear with no hyphae Better growth on chocolate than BAP Growth better on EMB than blood: or Rapid trehalose positive at 42 o C Spherical pleomorphic budding yeast with no hyphae Urea Phenol (Caffeic Acid) Z217 E14, G25 Z205 L65 Z118, G213 Cannot differentiate from C. gattii Anaerobes - Gram-negative Organism Test Catalog No. Bacteriodes fragilis group Colonial morphology on BAP/LKV A60 Colonial morphology on Bacteriodes ureolyticus Colonial morphology on BAP/LKV agar A60 Bilophila wadsworthia Colonial morphology on BAP/LKV A60 Z262 Fusobacterium nucleatum Colonial morphology on BAP/LKV A60 Porphyromonas spp. Colonial morphology on BAP/LKV A60 A30 Prevotella intermedia Colonial morphology on BAP/LKV A60 A30 Prevotella spp. Veillonella spp. Colonial morphology on BAP A30

7 Anaerobes - Gram-positive Organism Test Catalog No. C. difficile Colonial morphology on BAP A30 Odor C. perfringens Colonial morphology on BAP A30 of beta-hemolysis on BAP C. septicum Colonial morphology on BAP A30 C. sordellii Colonial morphology on BAP A30 C. tetani Colonial morphology on BAP A30 Peptostreptococcus spp. Colonial morphology on BAP A30 Propionibacterium acnes Colonial morphology on BAP A30 Z62 Rapid MGP Media (Cat. no. Z225) to rule out non-pathogenic Enterococcus spp. Results in five hours. Germ Tube Cryo (Cat. no. Z217) a ready-to-use germ tube solution made up of stabilized animal serum, comes pre-measured in vials. No mixing, measuring, or aliquoting is required! For Candida albicans. Nitrocef Matchbook (Cat. no. Z108) or Nitrocef Disk (Cat. no. Z7301) after a brief incubation, the positives will turn red/ orange as shown in the match-stick on the bottom. Negatives remain a buff color. Detects resistance to beta lactam drugs. Abbreviated Identification of Bacteria and Yeast; Approved Guideline -- Second Edition

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