Oscillatoria sp. PCC 6407 fresh water, USA (1964) 1. Oscillatoria sp. PCC 6412 fresh water, USA (1964) 1

Transcription

1 Table S1. Cyanobacterial strains and environmental samples used in the study. All numbered strains are maintained at the Helsinki University Cyanobacteria Culture Collection, all PCC strains are maintained at the Pasteur Culture collection of Cyanobacteria, Institut Pasteur. Environmental samples were collected by Finnish Environment Institute. An., Anabaena; Aph, Aphanizomenon; na, not analysed Strains Origin (year of isolation) Reference Anabaena flos-aquae 14 Lake Sääskjärvi, Finland (1985) 4 Anabaena flos-aquae 37 Lake Sääskjärvi, Finland (1985) 4 Anabaena circinalis 54 Lake Sääskjärvi, Finland (1986) 4 Anabaena circinalis 86 Lake Villikkalanjärvi, Finland (1986) 4 Anabaena sp. 90 Lake Vesijärvi, Finland (1986) 5 Anabaena sp. 130 Lake Säyhteenjärvi, Finland (1986) 4 Aphanizomenon sp. 3 Lake Långsjön, Finland (1985) 3, 4 Oscillatoria sp. 193 Lake Hormajärvi, Finland (1986) 4 Oscillatoria sp. PCC 6407 fresh water, USA (1964) 1 Oscillatoria sp. PCC 6412 fresh water, USA (1964) 1 Oscillatoria sp. PCC 6506 source unknown, USA 1 Oscillatoria sp. PCC 9029 source unknown 1 Oscillatoria sp. PCC 9107 soil, The Netherlands 1 Oscillatoria sp. PCC 9240 a Lake Hormajärvi, Finland (1986) 1 Oscillatoria sp. PCC Lake Levrasjön, Sweden (1981) 1 Oscillatoria sp. PCC Tarn River, France (2005) 2 Oscillatoria sp. PCC Tarn River, France (2005) 2 Oscillatoria sp. PCC Tarn River, France (2006) 2 Environmental samples Origin (year of isolation) Dominant species by microscopy 3915 Lake Kirkkojärvi (2004) An. lemmermannii 3916 Lake Kyrösjärvi (2004) An. lemmermannii, Woronichinia naegeliana 3922 River Ruskonjoki (2004) An. planctonica, Anabaena spp Lake Ranuanjärvi (2004) Aph. cf.yezoense 3978 Baltic Sea, Luvia, Finnish coast (2004) Oscillatoriales 4016 Lake Kymijärvi (2004) Planktothrix agardhii, Anabaena spp.

2 4334 Lake Pyhäjärvi (2005) Microcystis wesenbergii, Aphanizomenon sp Baltic Sea, Halikko, Finnish coast (2005) Aph. yezoense, Anabaena lemmermannii 9058 Baltic Sea, Helsinki, Finnish coast (2009) na a First described as Oscillatoria sp. 193

3 Figure S1. Restriction fragment length polymorphism (RFLP) analysis of anac amplicons (366 bp) of cyanobacterial strain mixes with HhaI and HinfI restriction enzymes. The amplicons mixed are indicated above the lanes according to the strain used for amplification: Anabaena sp. 37 (A37), Oscillatoria sp. PCC 6506 (6506), Oscillatoria sp. PCC 9107 (9107), and Aphanizomenon sp. 3 (Aph). Digestion reactions without the enzyme are indicated with ud, and the size marker lanes with SM.

4

5 Figure S2. Ion chromatograms of protonated anatoxin-a (ana; 166 m/z) and homoanatoxin-a (hana; 180 m/z) of the commercial anatoxin-a standard and the cyanobacterial strains studied.

6

7 Figure S3. MS 2 fragmentation patterns of the protonated anatoxin-a (166 m/z) and homoanatoxin-a (180 m/z) ions of the commercial anatoxin-a standard and the cyanobacterial strains studied. References 1. Aráoz, R., H. - O. Nghiêm, R. Rippka, N. Palibroda, N. Tandeau de Marsac, and M. Herdman Neurotoxins in axenic oscillatorian cyanobacteria: coexistence of anatoxin-a and homoanatoxin-a determined by ligand-binding assay and GC/MS. Microbiol. 151: Cadel-Six, S., C. Peyraud-Thomas, L. Brient, N. Tandeau de Marsac, R. Rippka, and A. Méjean Different genotypes of anatoxin-a-producing cyanobacteria coexist in the Tarn River, France. Appl. Environ. Microbiol. 73: Rapala, J., K. Sivonen, R. Luukkainen, and S. I. Niemelä Anatoxin-a concentration in Anabaena and Aphanizomenon under different environmental conditions and comparison of growth by toxic and non-toxic Anabaena-strains a laboratory study. J. Appl. Phycol. 5: Sivonen, K., K. Himberg, R. Luukkainen, S. I. Niemelä, G. K. Poon, and G. A. Codd Preliminary characterization of neurotoxic cyanobacteria blooms and strains from Finland. Tox. Assess. 4: Sivonen, K., M. Namikoshi, W. R. Evans, W. W. Carmichael, F. Sun, L. Rouhiainen, R. Luukkanen, and K. L. Rinehart Isolation and characterization of a variety of microcystins from seven strains of the cyanobacterial genus Anabaena. Appl. Environ. Microbiol. 58: