Molecular Mechanism and Regulation in Cation Transport ATPases and Related Genetic Diseases

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1 Molecular Mechanism and Regulation in Cation Transport ATPases and Related Genetic Diseases Program Thursday, June 15 at RIHGA ROYAL HOTEL KYOTO 17:00-19:00 Registration for SYMPOSIUM (in front of the hall Le Cygne in RIHGA ROYAL) 19:00-21:00 Get Together (buffet style dinner) in the hall Le Cygne in RIHGA ROYAL Friday, June 16 Registration 8:30 ~ (Buzz Hall on the basement in Building 4, West Area) Poster Placement 8:30 ~ (Lobby in front of Buzz Hall in Building 4, West Area) Opening Remarks 9:00-9:10 Hiroshi Suzuki, Kazuya Taniguchi, Chikashi Toyoshima Atomic structures of cation pumps S-1 9:10-9:55 Chikashi Toyoshima, Chair (University of Tokyo, Japan) Current status of calcium pump crystal structure analysis S-2 9:55-10:30 Jesper V. Møller (University of Aarhus, Denmark) Structural aspects of the modulatory and catalytic modes of ATP by the sarcoplasmic calcium pump S-3 10:30-11:00 Yuji Sugita (University of Tokyo, Japan) Molecular dynamics simulations of sarcoplasmic reticulum calcium pump in membrane S-4 11:00-11:35 Svetlana Lutsenko (Oregon Health and Science University, USA) Inactivation of copper-transporting ATPase ATP7B: Structural basis and functional consequences LUNCH 11:35-13:00 (Banquet Hall on the basement in Building 4, West Area)

2 Ion binding sites and Transport pathway S-5 13:00-13:40 David C. Gadsby, Chair (Rockefeller University, USA) Ion permeation through the Na,K-ATPase S-6 13:40-14:15 Jean-Daniel Horisberger (University of Lausanne, Switzerland) Extracellular cation access to their binding site in Na,K-ATPase S-7 14:15-14:35 Toshiaki Imagawa (Hokkaido University, Japan) Molecular architecture of Na binding pocket in Na,K-ATPase S-8 14:35-14:55 Magotoshi Morii (Toyama University, Japan) Proton pathway of the gastric H +,K + -ATPase COFFEE BREAK 14:55-15:15 (in front of Buzz Hall) Structure/function relationship and Cation transport mechanism (Buzz Hall in Building 4) S-9 15:15-15:55 Giuseppe Inesi, Chair (University of Maryland School of Medicine, USA) Binding and dissociation of nucleotide and Ca 2+ through sequential conformational states of the Ca 2+ ATPase (SERCA) S-10 15:55-16:30 Jens Peter Andersen (University of Aarhus, Denmark) Structure-function relationship of SR Ca 2+ -ATPase from mutational analysis S-11 16:30-17:05 Steven J. D. Karlish (Weizmann Institute of Science, Israel) Purification of Na,K-ATPase complexes expressed in Pichia Pastoris S-12 17:05-17:25 Jun Nakamura (Tohoku University, Japan) Difference in sensitivity of the sarcoplasmic reticulum Ca 2+ -ATPase molecules to polyols DINNER 17:25-19:30 (Banquet Hall on the basement in Building 4, West Area) S-13 19:30-20:05 Philippe Champeil, Chair (DBJC SBFM, France) Effect of thapsigargin or other ATPase inhibitors on the opening toward the aqueous medium of the Ca 2+ binding sites in SERCA1a S-14 20:05-20:40 David B. McIntosh (University of Cape Town, South Africa) The ATP-binding and phosphorylation transitions and energy coupling of Ca 2+ -ATPase S-15 20:40-21:20 Hiroshi Suzuki (Asahikawa Medical College, Japan) Molecular mechanism of Ca 2+ -ATPase and Darier disease caused by its defects

3 Saturday, June 17 Subunit interactions and Functional unit of pumps I S-16 9:00-9:40 Amir Askari, Chair (Medical University of Ohio, USA) Na + /K + -ATPase: Role of β-subunit in targeting of the enzyme to caveolae S-17 9:40-10:15 Ernst Bamberg (Max-Planck Institute of Biophysics, Frankfurt, Germany) Dynamics of subunit interaction of the Na + pump by FRET voltage clamp fluorometry S-18 10:15-10:50 Jack H. Kaplan (University of Illinois at Chicago, USA) Subunit interactions and membrane delivery of the Na,K-ATPase COFFEE BREAK 10:50-11:05 (in front of Buzz Hall) Subunit interactions and Functional unit of pumps II S-19 11:05-11:40 Kazuya Taniguchi, Chair (Hokkaido University, Japan) High affinity ATP binding to pig kidney Na/K-ATPase induced quarter site phosphorylation and activation of Na + -dependent p-nitrophenyl phosphate hydrolysis and removal of low affinity bound ATP induced inhibition of Na + -dependent ATP hydrolysis S-20 11:40-12:00 Yutaro Hayashi (Kyorin University, Japan) Isolation and characterization of tetraprotomeric and diprotomeric Na + /K + -ATPase S-21 12:00-12:20 Shunji Kaya (Hokkaido University, Japan) Oligomeric structure and regulation of enzymatic activity of P-type ATPases Photo-taking 12:20-12:30 (the quad (or Atrium) in Building 1, East Area) LUNCH 12:30-13:35 (Banquet Hall on the basement in Building 4, West Area) Regulation of cation pumps and Specific inhibitors I S-22 13:35-14:15 Flemming Cornelius, Chair (University of Aarhus, Denmark) Regulation of Na,K-ATPase by FXYD proteins controlled by protein kinases A handle on the most dynamic part of the Na,K-ATPase S-23 14:15-14:50 Käthi Geering (University of Lausanne, Switzerland) FXYD3 and FXYD6, new regulators of Na,K-ATPase S-24 14:50-15:25 Haim Garty (Weizmann Institute of Science, Israel) Regulation of the Na + /K + -ATPase by FXYD proteins

4 COFFEE BREAK 15:25-15:40 (in front of Buzz Hall) Regulation of cation pumps and Specific inhibitors II S-25 15:40-16:20 George Sachs, Chair (University of California Los Angeles, USA) Molecular modeling of the structure of the gastric H,K ATPase S-26 16:20-16:55 Jan Joep H.H.M. De Pont (Nijmegen Center for Molecular Life Sciences, Radboud University, Holland) The ouabain-binding site in Na,K-ATPase elucidated by chimeric approaches S-27 16:55-17:30 Jeffrey Froehlich (University of Maryland, School of Medicine, USA) The role of intermolecular conformational and chemical coupling in regulation of the Ca- and Na,K-ATPases by phospholamban and PLMS S-28 17:30-18:05 Carlos H. Pedemonte (University of Houston, USA) Regulation of proximal tubule Na,K-ATPase by dopamine DINNER (BANQUET) 18:05-20:30 (Atrium on the 1st floor in Building 1, East Area) 20:30-22:30 POSTER SESSION (Lobby in front of Buzz Hall in Building 4, West Area)

5 Sunday, June 18 Dysfunction of pumps and Diseases S-29 9:00-9:40 Kathleen J. Sweadner, Chair (Massachusetts General Hospital, USA) Mutation, deletion, and suppression of Na,K-ATPase subunits: from neurology to cells S-30 9:40-10:15 Bente Vilsen (University of Aarhus, Denmark) Na +,K + -ATPase structure-function relationship and pathophysiology studied by mutagenesis S-31 10:15-10:50 Ernesto Carafoli (University of Padova, Italy) The isoforms of the plasma membrane calcium pump in health and disease Physiological roles of pumps and Evolution Hiroshi Suzuki, Chair (Asahikawa Medical College, Japan) Introduction S-32 10:50-11:20 Leopoldo de Meis (Federal University of Rio de Janeiro, Brazil) Identification of a Ca 2+ -ATPase in brown adipose tissue mitochondria: Regulation of thermogenesis by ATP and Ca 2+ COFFEE/Early LUNCH BREAK 11:20-12:10 (Banquet Hall in Building 4, West Area) S-33 12:10-12:45 Zijian Xie, Chair (Medical University of Ohio, USA) Identification of a signaling pool of Na/K-ATPase S-34 12:45-13:15 Anita Aperia (Karolinska Institute, Sweden) Identification of a signaling motif in the Na,K-ATPase alfa subunit and its role for regulation of cell proliferation and apoptotic threshold S-35 13:15-13:45 Kunio Takeyasu (Kyoto University, Japan) Evolutionary process of the P-type ATPase and the biological significance of the β subunit S-36 13:45-14:10 Kiyoshi Kawakami (Jichi Medical University, Japan) Involvement of Na,K-ATPase alpha2 subunit in regulation of neural activities and synaptic connection during perinatal period S-37 14:10-14:30 Hideki Sakai (University of Toyama, Japan) Regulation of the gastric H,K-ATPase activity by CLC-5 S-38 14:30-14:50 Yukichi Hara (Tokyo Medical and Dental University, Japan) Evolution of Na/K-ATPase:Molecular cloning of Na/K-ATPase from protists Closing Remarks 14:50-15:00 Hiroshi Suzuki, Kazuya Taniguchi, Chikashi Toyoshima