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13 Supplemental Table 1. Primers used for qrt-pcr analysis Name abf2rtf abf2rtr abf3rtf abf3rtf abf4rtf abf4rtr ANAC096RTF ANAC096RTR abf2lp abf2rp abf3lp abf3lp abf4lp abf4rp ANAC096F(XbaI) ANAC096R(BamHI) ANAC096F(SalI) ANAC096R(SpeI) ANAC096 CF(XbaI) ANAC096 CR(BamHI) GAL4F GAL4R GAL4NF GAL4NR GAL4CF GAL4CR RD29ApF RD29ApR RD29A-50[1A]F RD29A-50[1A]R RD29A-50[2A]F RD29A-50[2A]R RD29A-50[1A/2A]F RD29A-50[1A/2A]R Nucleotide sequence (5' to 3') ATCCAGTTATTAGGTGGAAGCAGA AAGATTTCCCATTACACCAGCGGC ATGGGGTCTAGATTAAACTTCAAG CTACCAGGGACCCGTCAATGTC AACAAGGGTTTTAGGGCTTGGATG AACAAGGGTTTTAGGGCTTGGATG ATGGGAAGTTCATGTTTACCTCCAG GGAGAAATCTGAGTAACCGAATA ATGGTAGTATGAATTTGGGGAATG TCACCAAGGTCCCGACTCTGTCC ATGGGGTCTAGATTAAACTTCAAG CTACCAGGGACCCGTCAATGTC ATGGGAACTCACATCAATTT CAACAACT TCACCATGGTCCGGTTAATGTCC GCTCTAGAATGGGAAGTTCATGTTTACCTCCAG CGGGATCCGGAGAAATCTGAGTAACCGAATA ACGCGTCGACATGGGAAGTTCATGTTTACCTCCA G GGACTAGTGGAGAAATCTGAGTAACCGAATA GCTCTAGAATGGGAAGTTCATGTTTACCTCCAG CGGGATCCTTAAAAAGCTCCCTTTAAATTTTGTA GGCATTCCATATGATGGGAAGTTCATGTTACCTCC A GGCATTCCATATGAATACGAAAATCCGAAA GGCATTCCATATGATGGGAAGTTCATGTTACCTCC A CGGCTGCAGCTAGGAGAAATCTGAGTAACCGAA GGCATTCCATATGAATACGAAAATCCGAAA CGGCTGCAGCTAAATTTTTCGATCTTTTCCGGTT GCTCTAGAAAAATGACCACATGATGGGCCAATAG ACGCGTCGACGAGTAAAACAGAGGAGGGTCTCA CT ATGACTTTGACGTCACACCACGAAAACAGACGC TTCAAAAATGTCCCTTT ACGCGTCGACGAGTAAAACAGAGGAGGGTCTCA CT ATGACTTTGACGTCACACAAAAAAAACAGA ACGCGTCGACGAGTAAAACAGAGGAGGGTCTCA CT ATGACTTTGACGTCACACAAAAAAAACAGACGC TTCAAAAATGTCCCTTT ACGCGTCGACGAGTAAAACAGAGGAGGGTCTCA CT

14 RD29A[CGT/AAA] p F ATGACTTTGAAAACACACCACG RD29A[CGT/AAA] p R ACGCGTCGACGAGTAAAACAGAGGAGGGTCTCA CT GST-ANAC096F CGGATCCCCATGGGAAGTTCATGTTTACCTCC GST-ANAC096R CTGGTCGACTCAAGTTTTGATCTCATTCTTCATAG C His-ANAC096NF(BamHI) CGGGAT CCATGG GAAGTTCATGTTTACCTCCAG His-ANAC096NR(EcoRI) GGAATTCTTAAAAAGCTCCCTTTAAATTTTGTA His- ANAC096N(R11A/E18A)F1(Ba mhi) CGGGATCCATGGGAAGTTCATGTTTACC His- ANAC096N(R11A/E18A)R1 GATGAGGGCTTCATCCGTAGGATGAAAGGCGAACCC His- ANAC096N(R11A/E18A)F2 GGGTTCGCCTTTCATCCTACGGATGAAGCCCTCATC His- ANAC096N(R11A/E18A)R2(Ec ori) GGAATTCTTAAAAAGCTCCCTTTAAATTTTGTGA His-ANAC096N(d2-10)F CGGGATCCATGCATCCTACGGATGAAGAACTCATCG His-ANAC096N(d2-10)R GGAATTCTTAAAAAGCTCCCTTTAAATTTTGTGA MBP-ABF2F CGAATTCGATGGTAGTATGAATTTGGGGAATG MBP-ABF2R CGCGTCGACTCACCAAGGTCCCGACTCTGTCC; MBP-ABF3F CGGGATCCATGGGGTCTAGATTAAACTTCAAG MBP-ABF3R ACGCGTCGACCTACCAGGGACCCGTCAATGTC MBP-ABF4F CGAATTCGGAACTCACATCAATTTCAACAACT MBP-ABF4R CGCGTCGACTCACCATGGTCCGGTTAATGTCC nvenus-abf2f CGGGATCCATGTACCCATACGATGTTCCA nvenus-abf2r GGAATTCTCACCAAGGTCCCGACTCTGTCC nvenus-abf3f CGGGATCCATGGGGTCTAGATTAAACTTCAAG nvenus-abf3r CGGGATCCCTACCAGGGACCCGTCAATGTC nvenus-abf4f CGGGATCCATGTACCCATACGATGTTCCA nvenus-abf4r GGAATTCTCACCATGGTCCGGTTAATGTCCT cvenus-anac096f CGGGATCCATGGGAAGTTCATGTTTACCTCCAG cvenus- ANAC096R GGAATTCCTAGGAGAAATCTGAGTAACCGAATA ANAC096pF GCTCTAGAATTCTCACTCTACCAAAATAATAAGGT ANAC096pR CATGCCATGGTTGCAACAACAAAGTAGAATACGA TG ANAC096F AGGCCGTGCCCCGTTA ANAC096R ATCGCATAGTCGATATTCATGCAT ACT2F TATGAATTACCCGATGGGCAAG ACT2R TGGAACAAGACTTCTGGGCAT RD29AF GATATCGACAAGGATGTGCCG RD29AR GTATCCAGGTCTTCCCTTCGC COR47F ACACCAACGGTCGCAACA COR47R TCCACGATCCGTAACCTCTGT NCED3F GCTGCGGTTTCTGGGAGAT NCED3R TTGAGAAGACGATAATGGCGG

15 HVA22DF HVA22DR RAB18F RAB18R RD29BF RD29BR HAI2F HAI2R ANAC019F(XbaI) ANAC019R(BamHI) CAAGGCGCAGCTTTTATCTACA GGACGCCGTGTTTCTTGAAC GACATATGGGCTAAGGAAATCGA CCCGACAAGCATCTTAATGCA TTCTGACCACACCAAACCCAT CAGCCAGTGCCTCATGTCC ACGGGCTATGGGACGTAGTG ACACATGCGCACCATCGTA GCTCTAGAATGGGTATCCAAGAAACTGAACCGT CGGGATCCCCATAAACCCAAACCCACCAACTTGC

16 Supplemental References Ashburner, M., Ball, C.A., Blake, J.A., Botstein, D., Butler, H., Cherry, J.M., Davis, A.P., Dolinski, K., Dwight, S.S., Eppig, J.T., Harris, M.A., Hill, D.P., Issel-Tarver, L., Kasarskis, A., Lewis, S., Matese, J.C., Richardson, J.E., Ringwald, M., Rubin, G.M., and Sherlock, G. (2000). Gene ontology: tool for the unification of biology. The gene Ontology Consortium. Nat. Genet. 25: Bader, G.D., Betel, D., and Hogue, C.W. (2003). BIND: the Biomolecular Interaction Network Database. Nucleic Acids Res. 31: Cutler, S.R., Rodriguez, P.L., Finkelstein, R.R., and Abrams, S.R. (2010). Abscisic acid: emergence of a core signaling network. Annu. Rev. Plant Biol. 61: Kerrien, S., Aranda, B., Breuza, L., Bridge, A., Broackes-Carter, F., Chen, C., Duesbury, M., Dumousseau, M., Feuermann, M., Hinz, U., Jandrasits, C., Jimenz, R.C., Khadake, J., Mahadevan, U., Masson, P., Pedruzzi, I., Pfeiffenberger, E., Porras, P., Raghunath, A., Roechert, B., Orchard, S., and Hermjakob, H. (2012). The IntAct molecular interaction database in Nucleic Acids Res. 40: D Lamesch, P., Berardini, T.Z., Li, D., Swarbreck, D., Wilks, C., Sasidharan, R., Muller, R., Dreher, K., Alexander, D.L., Garcia-Hernandez, M., Karthikeyan, A.S., Lee, C.H., Nelson, W.D., Ploetz, L., Singh, S., Wensel, A., and Huala, E. (2012). The Arabidopsis Information Resource (TAIR): improved gene annotation and new tools. Nucleic Acids Res. 40: D Stark, C., Breitkreutz, B.J., Reguly, T., Boucher, L., Breitkreutz, A., and Tyers, M. (2006). BioGRID: a general repository for interaction datasets Nucleic Acids Res. 34: D Umezawa, T., Nakashima, K., Miyakawa, T., Kuromori, T., Tanokura, M., Shinozaki, K., and Yamaguchi-Shinozaki, K. (2010). Molecular basis of the core regulatory network in ABA responses: sensing, signaling and transport. Plant Cell physiol. 51:

17 Yilmaz, A., Mejia-Guerra, M.K., Kurz, K., Liang, X., Welch, L., and Grotewold, E. (2011). AGRIS: the Arabidopsis Gene Regulatory Information Server, an update, Nucleic Acids Res. 39: D

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