Membrane Potentials. Why are some cells electrically active? Model 1: The Sodium/Potassium pump. Critical Thinking Questions
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1 Membrane Potentials Model 1: The Sodium/Potassium pump Why are some cells electrically active? 1. What ion is being moved out of the cell according to model 1? a. How many of these are being moved out? b. Is this ion positively or negatively charged? 2. What ion is being brought into the cell according to model 1? a. How many of these are being moved in? b. Is this ion positively or negatively charged?
2 3. After this pump runs 5 times, how many positive ions will be moved outside the cell? a. How many positive ions will have been brought into the cell after 5 cycles of the pump? b. What is the difference in positive ions between the inside and the outside of the cell after 5 cycles? c. What is the difference in positive ions between the inside and the outside of the cell after 70 cycles? 4. Imagine instead of pumping ions, we are pumping dollars. Is the cell spending more (pumping out) than it brings in? a. If you spend more than you bring in, what happens to your bank balance? 5. In the space below draw a line to represent the cell membrane. Label one side inside and the other side outside. Place + symbols on the side of the membrane that would have the positive bank account (according to our analogy above) and put symbols on the side of the membrane that would have the negative bank balance. Model 2: A polarized membrane
3 Resting Membrane Potential (mvolts) 6. Place a large + sign on the side of the membrane with the most positive charges and a sign on the side of the membrane with the least number of positive charges. 7. Where are most of the sodium ions, inside or outside the cell? 8. If the channel were to open, which way would sodium travel through the channel based on the laws of diffusion? 9. What effect would this have on the membrane potential (the difference in charge across the membrane)? Time (milliseconds) 10. This chart shows the resting membrane potential of a nerve cell. Notice that the line is running at around -70 millivolts. a. The resting membrane potential has a negative value. Based on what we ve already learned - is this measuring the inside or outside of the cell? [i.e. which one is negative with respect to the other?] b. If a sodium channel opens, will the inside of the cell become more positive or more negative as the sodium ions come in along their concentration gradient? c. Based on your answer to a and b, extend the line in the chart to show what might happen to the potential when sodium channels open. d. Before the sodium channel opened, the membrane was polarized, like a battery (a positive and negative side). After opening the sodium channel, is the cell going to be polarized anymore?
4 Model 3: A depolarized membrane 11. What has changed about the sodium channel in this model (compared to model 2)? 12. How does the number of positive ions inside the cell compare with the number outside the cell? 13. Compare this model to model 2 which contained a polarized membrane. Based on the ion distribution in these two models, devise definitions as a group, for the following: Polarized Depolarized 14. Based on the concentration of potassium ions inside and outside the cell, which way do you think K+ ions would go if the potassium ion channel were to open and the sodium channel were to close? [hint: draw it if you need to] 15. Would this depolarize or repolarize the membrane?
5 Exercises 1. Find the points on this graph where membrane potential went up but went back down again without causing a drastic change in the polarity of the cell. Label that portion of the graph Local Depolarization. 2. Draw an arrow at the place on the graph where the entire membrane started to depolarize [hint: it s when potential starts to rise as fast as it can]. a. About what voltage was achieved before the membrane started to actually depolarize (i.e. where your arrow is pointing) b. Threshold is also called the point of no return, and refers to the voltage at which the entire membrane will depolarize. Based on your answers to 1 2a, draw a dotted line across the membrane at the voltage that represents the threshold voltage. 3. Circle the point on the graph where the potassium channels open and the sodium channels close. 4. Sometimes the potassium channels stay open long enough that the membrane will hyperpolarize. Indicate on the graph where the membrane is hyperpolarized.
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