Dr. Efthimia Mina Tsagris Personal: Fellowships and competitive awards: Professional Experience: Research Interests

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1 Dr. Efthimia Mina Tsagris Ass. Professor Department. of Biology Division Molecular Biology, Cell Biology and Development P.O. Box 2208 University of Crete Heraklion Crete Greece Tel , fax Personal: Date and Place of Birth 17 May 1955 Athens, Greece two children Education: Degree: October 1981, Diploma in Chemistry, University of Giessen, F.R.G PhD: 1987, PhD in Molecular Plant Virology, University of Giessen and Max-Planck Institute for Biochemistry, F.R.G. Fellowships and competitive awards: : PhD fellowship from the Max-Planck Society : Research fellowship from the Max-Planck Society : EMBO Long Term Fellowship Professional Experience: Positions: : Research Assistant, Max-Planck Institute for Biochemistry : Postdoctoral fellow at the Institute of Molecular Biology and Biotechnology (IMBB), Crete, Greece : Assistant Research Scientist, IMBB. ( Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology) Cooperating Ass. Res. Scientist, IMBB 1992-till now : Assistant Professor, University of Crete ( tenure since 1998) Research Interests The following topics are being studied using methods of molecular biology, molecular genetics and plant transformation: Plant pathogen interactions at the molecular level : Studies on in vitro replication of viroid RNA by plant RNA polymerases : Studies on in vitro processing of viroid RNAs in cell free extracts from plant cells and on autocatalytic processing Potyviruses: host and viral factors involved in replication Posttranscriptional Gene silencing and transgenic resistance to agronomically important viruses till now viroid replication and processing, host factors involved in viroid life cycle.

2 Teaching: Undergraduate course Plant Molecular Biology and RNA (26 hrs), Molecular Plant Virology (26 hrs) Graduate Course Plant Molecular Biology and Biotechnology Course Molecular Phytopathology 11 h Participation in the Course Molecular Diagnosis in Plant Viruses (4h) (till 2009) And Transgenic Plants ( 4h) Organization of Meetings Member of the Organization Comitee of the 3rd Hellenic Virological Congress. Member of the Organization Comitee of the 8th Hellenic Phytopathological Congress. Organization ( with Dr. Martin Tabler, IMBB ) of the EMBO Workshop on Plant Viruses Virus Invasion and Host Defence May 28-June 1 st 2000 Kolymbari Crete Greece Grants 12 international and national Grant applications successful. (Coordinator in 3 of them) (On request excel file available) Referee: I have been referee for the following international peer reviewed Journals: VIROLOGY Journal of General Virology, Journal of Virology Biotechnology Progress Plant Biotechnology Journal Transgenic research Plant Journal Plant Cell ISRAEL-USA cooperation programmebard, evaluator for EU Grant Applications (FAIR and Inco-Dev) evaluation for Research Grant Applications of the Ministry of Development, Greece. Evaluation of research grants of Greek Universities ( Ioannina, Athens)

3 Publications in international peer-reviewed journals Tsagris M., Tabler M., and Saenger H.L.. (1987) Oligomeric potato spindle tuber viroid (PSTV) RNA does not process autocatalytically under conditions where other RNAs do. Virology, 157, Tsagris M., Tabler M.,Muehlbach H.P. and Saenger H.L. (1987) Linear oligomeric potato spindle tuber viroid (PSTV) RNAs are accurately processed in vitro to the monomeric circular viroid proper when incubated with a nuclear extract from healthy potato cells. EMBO J., 8, Tabler M., Tsagris M. (1990) Viroid Replication Mechanisms. In Fraser, R.S. (ed), Recognition and Response in Plant - Virus Interactions, NATO ASI series Vol. H41: Springer Verlag, Heidelberg and New York, Tsagris M., Tabler M. and Saenger H.L. (1991) Ribonuclease T1 generates circular RNA molecules from viroid-specific RNA transcripts by cleavage and intramolecular ligation. Nucleic Acids Res., 19, Tabler M. Tsagris M. (1991) Catalytic antisense RNAs produced by incorporating ribozyme cassettes into cdna. GENE, 108, Tabler M., Tzortzakaki S. and Tsagris M. (1992) Processing of longer than unit length potato spindle tuber viroid RNAs into infectious monomeric circular molecules by a G-specific endoribonuclease. Virology, 190, Steger, G., Baumstark, T., Moerchen, M., Tabler, M., Tsagris, M., Saenger, H.L. and D. Riesner (1992) Structural requirements for viroid processing by RNase T1 Journal of Molecular Biology, 227, Prombona, A., Tabler, M., Providaki, M. And Tsagris, M. (1995) Structure and expression of LeMA-1, a tomato protein that belongs to the SEC18- PAS1- CDC48- TBP-1 protein family of putative Mg 2+ dependent ATPases. Plant Mol. Biol.27, R. Saegesser, E. Martinez, M. Tsagris and M. Tabler Detection and isolation of RNA binding proteins by RNA ligand screening of a cdna expression library, (1997) Nucl. Acids Res.25, Tabler, M. and Tsagris, M. (1997) Cloning strategies for catalytic antisense RNAs. Ribozyme Protocols (ed. Phil Turner),

4 In "Methods in Molecular Biology" (Series ed John Walker), Humana Press Inc., Totowa, N.J., USA, Vol 74, p Tabler, M., Tsagris, M. and Hammond, J. (1998) Antisense RNA and ribozyme - mediated resistance to plant viruses In Hadidi (ed), Control of Plant Virus Diseases, APS Press, Minnesota, USA, p79-94 Manoussopoulos IN, Maiss E, Tsagris M. Native electrophoresis and Western blot analysis (NEWeB): a method for characterization of different forms of potyvirus particles and similar nucleoprotein complexes in extracts of infected plant tissues. J Gen Virol Sep;81 Pt 9: Liu B, Tabler M, Tsagris M. Episomal expression of a hammerhead ribozyme directed against plum pox virus. Virus Res Jun; 68(1): Denti MA, Martinez de Alba AE, Sagesser R, Tsagris M, Tabler M. A novel RNA-binding protein from Triturus carnifex identified by RNA-ligand screening with the newt hammerhead ribozyme. Nucleic Acids Res Mar 1; 28(5): Girgis, S.M., Bem, F., Kyriakopoulou, P.E., Dovas, C.I., Sklavounos, A.P., Avgelis, Katis, N.A., Tzortzakaki, S., Tsagris, M. A new Ilarvirus isolated from grapevine in Greece. Plant Disease.Dec v. 84 (12) Boutla A, Delidakis C, Livadaras I, Tsagris M, Tabler, M Short 5'-phosphorylated double-stranded RNAs induce RNA interference in Drosophila. Curr Biol Nov 13; 11(22): Papaefthimiou I, Hamilton A, Denti M, Baulcombe D, Tsagris M, Tabler M. Replicating potato spindle tuber viroid RNA is accompanied by short RNA fragments that are characteristic of post-transcriptional gene silencing. Nucleic Acids Res Jun 1; 29(11): Kalantidis K, Psaradakis S, Tabler M, Tsagris M. The occurrence of CMV-specific short RNAs in transgenic tobacco expressing virus-derived double-stranded RNA is indicative of resistance to the virus. Mol Plant Microbe Interact Aug;15(8): Boutla A, Kalantidis K, Tavernarakis N, Tsagris M, Tabler M. Induction of RNA interference in Caenorhabditis elegans by RNAs derived from plants exhibiting post-transcriptional gene silencing. Nucleic Acids Res Apr 1;30(7): Tabler, M., Boutla,A., Kalantidis,K. and Tsagris,M. (2003) Short interfering and micro RNAs: tiny but mighty in Non-coding RNAs (eds. Barciszewski, J. and Erdmann V.) Landes Bioscience, Georgetown, Texas USA, pp

5 Gozmanova M, Denti MA, Minkov IN, Tsagris M, Tabler M. Characterization of the RNA motif responsible for the specific interaction of potato spindle tuber viroid RNA (PSTVd) and the tomato protein Virp1. Nucleic Acids Res Oct 1;31(19): Martinez de Alba AE, Sagesser R, Tabler M, Tsagris M. A bromodomain-containing protein from tomato specifically binds potato spindle tuber viroid RNA in vitro and in vivo. J Virol Sep;77(17): Maniataki E, Martinez de Alba AE, Saegesser RS, Tabler M, Tsagris M. Viroid RNA systemic spread may depend on the interaction of a 71-nucleotide bulged hairpin with the host protein VirP1. RNA Mar;9(3): Erratum in: RNA Apr;9(4):502.. Denti M.A, Boutla A., Tsagris M. and Tabler M. (2004) Short interfering RNAs specific for potato spindle tuber viroid are found in the cytoplasm but not in the nucleus. The Plant Journal, 37, Missiou A., Kalantidis K. *#, Boutla A. Tzortzakaki S., Tabler M. and Tsagris M. # (2004) Expression of double-stranded RNA in potato confers complete and broad viral resistance to potato virus Y (PVY) that remains unaffected by infections with potato virus X (PVX) Molecular Breeding, 14, Tabler M, Tsagris M. Viroids: petite RNA pathogens with distinguished talents. Trends Plant Sci Jul;9(7): Spontaneous short range silencing of a GFP transgene in Nicotiana benthamiana is possibly mediated by small quantities of sirna that do not trigger systemic silencing Kalantidis K., Tsagris M. and Tabler M. The Plant Jounal, 2006, Mar:45 (6): Virp1 is a host protein with a major role in Potato spindle tuber viroid infection in Nicotiana plants. Kalantidis, K., Denti, M.A., Tzortzakaki, S., Marinou, E., Tabler, M. and Tsagris, M. (2007) J Virol. 81: Viroids Tsagris, E. M., Martinez de Alba, A. E., Gozmanova, M., Kalantidis. ( 2008). Viroids. Cell Microbiol. 10, Manousopoulos I.N. and Tsagris M. (2009) Native Electrophoresis and Western Blot Analysis (NEWeB): Methods and Applications Methods Mol Biol 536,

6 Girgis S.I, Bem F.P., Dovas Ch., Sclavounos A., Avgelis A., Tsagris M., Katis N., Kyriakopoulou P. ( 2009) Characterization of a novel ilarvirus causing grapevine angular mosaic disease Europ. J. Plant Pathol. 125 (2), Vachev T., Ivanova D.,Minkov I., Tsagris M., Gozmanova M. Trafficking of Potato spindle tuber viroid between tomato and Orobanche ramosa Virology, (2010), 399, Kotakis Ch., Vrettos N., Kotsis D., Tsagris M., Kotzabasis K. and Kalantidis K. (2010) Light intensity affects RNA silencing in transgenic Nicotiana benthamiana plants BMC Plant Biology, 10:220 Dalakouras, A.,Tzanopoulou, M., Tsagris, M., Wassenegger, M., Kalantidis, K.(2010) Hairpin transcription does not necessarily lead to efficient triggering of the RNAi pathway, Transgenic Res, 26 June, Publication in refereed Conference Proceedings M.Gozmanova, M.Denti, I.Minkov, M.Tsagris and M.Tabler. (2005) Study of the affinity and specificity of interaction of the PSTVd and tomato protein Virp1, Balkan Scientific conference of Biology, Plovdiv, May 19-21, Proceedings, Section Molecular Biology, pages Kalantidis K., Tsaftaris A., Manousopoulos I., Tzortzakaki S. and Tsagris M. (2000) Generation of 13 K gene sugar beet transformants and evaluation of their resistance to BNYVV infection. Conference Proceedings Phytosphere 99 European Plant Biotechnology Network G. de Vries and K Metzlaff eds. p Tsagris, M., Fragkiadakis, G., Roubelakis-Angelakis, K.A. and Tabler, M. (1990) Viroids in Grapevine Cultivars in Greece. In: Rumbos, I.C., Bovey, R., Gonsalves, D., Hewitt, W.B. and Martelli, G.P. (eds), Proceedings of the 10 th Meeting of the International Council for the Study of Viruses and Diseases of the Grapevine (ICVG). ORES Publishing, Volos, Greece, Katis, N., Hatziloukas, S., Tsagris, M., Roumbos, I.C. and Roubelakis-Angelakis, K.A.(1990) Presence of closteroviruses and viroids in grapevine varieties with symptoms of leaf-roll and stem-pitting diseases. In: Rumbos, I.C., Bovey, R., Gonsalves, D., Hewitt,W.B. and Martelli, G.P. (eds), Proceedings of the 10 th Meeting of the ICVG. ORES Publishing, Volos, Greece, Abstracts ( not yet published) Kalantidis K., Boutla A., Missiou A., Providaki M., Trandas E., Tzanopoulou M., Tzorztakaki S., Holeva R., Mavrakis S., Tabler M., Tsagris M. (2008)

7 Transgenic plants expressing dsrna derived from viral sequences: a method to induce a natural defence mechanism leading to virus resistant plants Phytopathol. Mediterr.47, Kotsis D., Kalantidis K., Holeva R., Tzortzakaki S., Tabler M. Tsagris M. (2008) Transgenic Nicotiana benthamiana plants resistant to plum pox virus. Phytopathol. Mediterr.47, Kotsis D., Holeva R., Kalantidis K., Tzortzakaki S., Tabler M., Tsagris M. ( 2009) Application of RNA silencing technology for the generation of transgenic plants resistant to plum pox virus: evaluation of resistance in T2 generation Phytopathol.Mediterr. 48, 324 Other Publications Ευθυμία Μίνα Τσαγρή (1994) Ιώσεις της αμπέλου Από τη μελέτη: Η αμπελουργία στην Κρήτη, προβλήματα και προοπτικές, Συντον. Κ.Α. Ρουμπελάκη- Αγγελάκη, ΕΣΕΤΕ και Γεωτεχνικό Επιμελητήριο της Ελλάδος, Ηράκλειο, Κρήτης σελ και

Virp1 Is a Host Protein with a Major Role in Potato Spindle Tuber Viroid Infection in Nicotiana Plants

Virp1 Is a Host Protein with a Major Role in Potato Spindle Tuber Viroid Infection in Nicotiana Plants JOURNAL OF VIROLOGY, Dec. 2007, p. 12872 12880 Vol. 81, No. 23 0022-538X/07/$08.00 0 doi:10.1128/jvi.00974-07 Copyright 2007, American Society for Microbiology. All Rights Reserved. Virp1 Is a Host Protein