iplanta COST Action CA15223 Workshop on "Biosafety issues associated with RNAi" 6-7 SEPTEMBER 2017 GHENT UNIVERSITY, FACULTY OF BIOSCIENCE ENGINEERING

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1 iplanta COST Action CA15223 Workshop on "Biosafety issues associated with RNAi" 6-7 SEPTEMBER 2017 GHENT UNIVERSITY, FACULTY OF BIOSCIENCE ENGINEERING GHENT (BELGIUM) FINAL PROGRAM Grosshans H, Filipowicz W.Nature. 2008

2 DAY 1 - WEDNESDAY 6 TH (Joint Session with IOBC), Ghent University, Faculty of Bioscience Engineering 8:45 Welcome 9:00 Session 1 SPECIFIC BIOSAFETY ISSUES ASSOCIATED WITH RNAi (WG3) INVITED LECTURE X. ZHOU, B.D.SIEGFRIED (University of Kentucky, University of Florida) J. B. SWEET (JT Environmental Consultants, UK) S. HALLER, F. WIDMER, B.D. SIEGFRIED, X. ZHOU, J. ROMEIS (Agroscope, CH; University of Florida, USA; University of Kentucky, USA) I. URRU, O. CHRISTIAENS, G. SMAGGHE, S. ARPAIA (ENEA, IT; Ghent University, BE) LESSONS AND PERSPECTIVES FROM THE EARLY-TIER RISK ASSESSMENT OF TRANSGENIC RNAI PLANTS ON NON-TARGET ARTHROPODS (Invited lecture) ORAL PRESENTATION INTRODUCTION TO RNAi RESEARCH AND DEVELOPMENT IN RELATION TO INVERTEBRATES AND CROPS SUSCEPTIBILITY OF TWO LADYBIRD SPECIES TO DSRNA TARGETING THE VATPASE A OF THE WESTERN CORN ROOTWORM ds RNAi EXTRACTS TARGETING THE CLATHRIN GENE OF COLORADO POTATO BEETLE HAVE NO ACUTE TOXIC EFFECTS ON THE NON-TARGET PREDATOR Chrysoperla carnea 40 min 10:40 Coffee break

3 11:00 Session 2 SPECIFIC BIOSAFETY ISSUES ASSOCIATED WITH RNAi (WG3) ORAL PRESENTATION B. ALSHUKRI, H. ABD EL HALIM, A.M. R. GATEHOUSE, M. EDWARDS. (Newcastle University, UK; Benha University, ET) W.JARAUSCH, M.RUNNE, M.WASSENEGGER, G.KRZCAL (ALPLANTA Institute For Plant Research, DE) J. POLÁK, J. K. KUNDU, P. KOMÍNEK, T. NEUBAUEROVÁ, R. SCORZA (Crop Research Institute, CZ; USDA-ARS, USA) OLIVIER CHRISTIAENS (Ghent University) dsrna TOWARDS VOLTAGE GATED ION CHANNELS CAUSE MORTALITY IN Tribolium castaneum BUT ARE NOT DETRIMENTAL TO Apis mellifera SURVIVAL OR IMMUNITY RNAi-MEDIATED CONTROL OF SPOTTED WING DROSOPHILA (Drosophila suzukii): EFFICACY CHALLENGES AND BIOSAFETY CONSIDERATIONS Monilia sp. INFECTION WEAKENS RESISTANCE OF TRANSGENIC PLUM Prunus domestica L., cv. HoneySweet TO THE PLUM POX VIRUS RNAi AS AN INSECT PEST CONTROL STRATEGY: BIOSAFETY CONSIDERATIONS 12:30 Closure of the IOBC meeting 12:45 14:00 Lunch 14:00 16:30 Lab demonstration (Ghent University)

4 DAY 2- THURSDAY 7 TH, Ghent University, Faculty of Bioscience Engineering 09:00 Session 1 SPECIFIC BIOSAFETY ISSUES ASSOCIATED WITH RNAi (WG3) ORAL PRESENTATION Z. MARTINEZ, O. CHRISTIAENS, M. GOMEZ TARDAJOS, P.DUBRUEL, G. SMAGGHE (Ghent University) Á. GALLÉ, K. TAKÁCS, J. CSISZÁR, J. GYÖRGYEY, J.KISS (University of Szeged, HU; Szent István University, Gödöllő, HU) I. ZAGRAI, L. ZAGRAI, M. RAVELONANDRO, R. SCORZA, Z. JAKAB, G. GUZU (Fruit Research Station, RO; INRA, FR) J. PEJIN, V. TUMBAS ŠAPONJAC, A. FIŠTEŠ, J. VULIĆ, M. PRIBIĆ (University of Novi Sad, SRB) M. RAVELONANDRO, I. ZAGRAI, L. ZAGRAI, R. SCORZA, A. CALLAHAN, C. DARDI CK, P. BRIARD (INRA, FR; Fruit Research Station, RO; USDA-ARS, USA) CONFOCAL MICROSCOPY ANALYSIS OF POLYMER-COATED DSRNA UPTAKE IN LEPIDOPTERA MIDGUT CELLS WOUNDING AND WATER STRESS ALTERS LATERAL ROOT FORATION VIA MIRNA REGULATED NETWORK: SOME INTERESTING MIRNAS IN Brachypodium distachyon: CONTRIBUTION TO THE ENVIRONMENTAL RISK ASSESSMENT OF RNAi BASED PLANTS PRELIMINARY ASSESSMENT ON THE POTENTIAL REDUCTION OF TREATMENTS WITH INSECTICIDES FOR APHIDS BY USING RNAi TECHNOLOGY IN PLUM AGAINST PLUM POX VIRUS ENGINEERING NUTRIENT CONTENT IN PLANTS THROUGH RNAi ARE THERE ANY ISSUES IN EATING FRUITS BEARING EITHER RNAi OR VIRUS? Coffee break Round table: Future perspectives of RNAi-GM plants. o Chair: Salvatore Arpaia o WG Leaders: Guy Smagghe, Bruno Mezzetti, Jeremy Sweet. o Science: Joe Zhou Lunch Meeting of the working group 3: planning future activities.

5 PERTICIPANTS INTERNATIONAL INVITED SPEAKER 1. Xuguo Zhou (University of Kentucky), WG3 SPEAKERS AND ORGANIZERS 2. Joerg Romeis, 3. Aleksandar 4. Jaroslav Polak, 5. Jeremy Sweet, 6. Isabella Urru, 7. Martin Edwards, 8. Wolfgang Jarausch, 9. Michel Ravelonadro, 10.Salvatore Arpaia, 11.Ioan Zagrai, 12.Agnes Galle, 13.Olivier Christiaens, 14.Guy Smagge, 15. Antje Dietz-Pfeilstetter, 16. Jozsef Kiss, 17. Bruno Mezzetti, 18. Silvia Sabbadini, IN COLLABORATION WITH:

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