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1 DOI: /jcprm : *,. 2,. 2,. 3, ,., 4,, (), Natnik48s@yandex.ru 2..,., 35, (), phenolic@ippras.ru 3,., 3,, 6345, ), eikor@mail.ru,,. 14 :, -. : K. scapigera K. rhombopilosa.-. :,,, -,, K. (Kalanchoe Adans.) 13.. [1]. -,,, [2, 3] ,,,, (),. -,,.: (495) , Natnik48s@yandex.ru,,.: (499) , p.lapshin@mail.ru,,.: (499) , nzagoskina@mail.ru,,.: (3822) , eikor@mail.ru,,.: (495) , misin@sky.chph.ras.ru;. - [2 4]. - [5, 6]. :, -. *,.
2 114.,.,.,.,. 34 (Kalanchoe L.),.., - -12, -. :,, -, ()., «-1-», [7]. -, - 1,3. (R OH)., R R + e + H +,, [8],. - R R +. - [8].,, - ( )., -, -,.. (- ) 1%. [9] - ( 2 ),,. 2 - : 2 + (1) HO 2 HO (4) [9] - 2, (1) (3). 2 (. 1) ,, 2, - * - - 2,,,,,, 3 N, S, Se--,, -, - 4 ), -, 2, - 2, - 2, -. :,. (2) (3)
3 115, - ( 2, HO 2 ), 2 : = (1 I/I ), I, I 2 -, () [9] ,. - 3%. ()» [1]., - in vivo. H 2 O 2 Hb,., (Hb( )Fe 4+ = O). -, LO 2 2, 3- ( 2 )*, 425. Hb-H 2 O 2 t, - [1]. «Lum- 5773». (k, -1 ), (t/t ) (m, ), : t/t = km+1, t. - 15%. 1 - m, 2 - t/t m, (k). 4 I,. m = ,5 2 15,2 1,3,4 5 t, t/t m,,2,4,6, () m,. 2. (t/t ) m,, 2-34., K. scapigera11 K. rhombopilosa 21 ( , ), - K. blossfeldiana K. laetivirens ( )., K. pinnata K. daigremontiana, (294 55, )., -, 14, (- ).
4 116.,.,.,.,. 2.,., 1 K.pinnata K.millotii 17 2 K.beauverdii K.fedtschenkoi 64 3 K.orgyalis K.serrata K.imperialis K.rhombopilosa K.tomentosa K.kalandiva 55 6 K.daigremontiana K.laciniata K.thyrsiflora K.tubiflora K.hildebrandtii K.longiflora 33 9 K.velutina K.bracteata K.beharensis v.aureoneu K.crenata K.scapigera K.nyikae K.citrina K.sedoides K.X kewensis 31 3 K.blossfeldiana K.serratifolia K.blossfeldiana mini K.germanae K.laetivirens K.syncepala K.figuereidoi K.gastonis-bonnieri K.pubescens 388., 3 2,., 1 (. 3), 8 (4, 8, 18, 21, 23, 28, 3, 33),, -, 4 1 (. 3). 2,. (R- OH) [9]: R-OH 2 + R ; (5) 2 + R-OH H 2 O 2 + R. (6), - 2,, (5) =+25 : 2 / = 2 / +,59lg(C2 / C). (7) ( 2 ) (5) (R-OH), C 2 -, (7), ,, - 2, () [11] , ) (. 4). 2, ) 4. 18, 2, 6,., -, 2,.,, r =, «Hb H 2 O 2» (k, -1 )., -, 4, - r =,89. (r =,81).
5 117 ) ). 3. () () (V=2 ), () () 1. -1, ; I ( 1,37 ) k, 1/ 12 1, (, ) ), (, ) (), (k, 1/) ) , [2, 3],. :,,, ;,,. 15,,. (35 4%), -,,,, :,, :,,,,. [2]. [3].,., : K.scapigera K.rhombopilosa- K.pinnata1 K.daigremontiana 6. -
6 118.,.,.,.,. - -,,,., :,. (r=,89). : K.scapigera K.rhombopilosa , , ,.,.,. : -, () //, ,.,. //, ,.,.,. Kalanchoe daigremontiana (Hamet de la Bbthie) Jacobs et Perr. // ,.,.,. - //, ,.,.,. - // // LII, Peyrat-Maillard M.N, Bonnely S., Berset C. Determination of the antioxidant activity of phenolic compoundsby coulometric detection // Talanta. 2. Vol. 51. Pp Korotkova E.I., Karbainov Y.A., Avramchik O.A. Investigation of antioxidant and catalytic properties of some biological-active substances by voltammetry // Anal. and Bioanal. Chem. 23. Vol. 375, N1-3. Pp ,.,.,.,. - // , ,.,. //
7 119 Sazhina N.N. 1*, Lapshin P.V. 2, Zagoskina N.V. 2, Korotkova E.I. 3, Misin V.M. 1 COMPARATIVE ANALYSIS OF KALANCHOE JUICE ANTIOXIDATIC ACTIVITY 1 N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina, 4, Moscow, (Russia), Natnik48s@yandex.ru 2 K.A. Timirjazev Institute of Plant Physiology, Russian Academy of Sciences, RAS, ul. Botanicheskaia, 35, Moscow (Russia), phenolic@ippras.ru 3 Tomsk Polytechnic University, pr. Lenina, 3, Tomsk, 6345, (Russia), eikor@mail.ru Now the special attention to studying of antioxidant activity of various herbs is given for the purpose of search among them the most active sources of biologically active components. In this work for this purpose the comparative analysis of measurement results of the total antioxidatic activity for juice of 14 different types of Kalanchoe (Kalanchoe L.) is carried out by three methods: ammetric, voltammetric and chemiluminescence. Among the studied samples two most active kinds of Kalanchoe are exposed: K.scapigera and K.rhombopilosa. They can appear more perspective sources of biologically active components in comparison with types which are used now. Keywords: antioxidants, ammetric, voltammetric, chemiluminescence methods, polyphenols, Kalanchoe. References 1. Semenova L.V., Iampol'skii Iu.V. Lekarstvennye ekzoticheskie rasteniia. [Medicinal exotic plants]. St. Petersburg, 23, no. 1, 125 p. (in Russ.). 2. Volzhanova M.I., Bail'man R.A., Suslina S.N., Bykov V.A. Voprosy biologicheskoi, meditsinskoi i farmatsevticheskoi khimii, 21, no. 7, pp (in Russ.). 3. Volzhanova M.I., Suslina S.N., Bykov V.A. Voprosy biologicheskoi, meditsinskoi i farmatsevticheskoi khimii, 211, no. 8, pp (in Russ.). 4. Anisimov M.M., Gerasimenko N.I., Chaikina E.L., Serebriakov Iu.M. Izvestiia RAN. Seriia biologicheskaia, 29, no. 6, pp (in Russ.). 5. Misin V.M., Sazhina N.N., Vandyshev V.V., Khomik A.S. Voprosy biologicheskoi, meditsinskoi i farmatsev-ticheskoi khimii, 21, no. 12, pp (in Russ.). 6. Olennikov D.N., Zilfikarov I.N., Toropova A.A., Ibragimov T.A. Khimiia rastitel'nogo syr'ia, 28, no. 4, pp (in Russ.). 7. Iashin A.Ia. Rossiiskii khimicheskii zhurnal, 28, vol. LII, no. 2, pp (in Russ.). 8. Peyrat-Maillard M.N, Bonnely S., Berset C. Talanta, 2, vol. 51, pp Korotkova E.I., Karbainov Y.A., Avramchik O.A. Anal. and Bioanal. Chem., 23, vol. 375, no. 1-3, pp Teselkin Iu.O., Babenkova I.V., Liubitskii O.B., Klebanov G.I., Vladimirov Iu.A. Voprosy meditsinskoi khimii, 1997, vol. 43, no. 2, pp Sazhina N.N., Misin V.M., Korotkova E.I. Khimiia rastitel'nogo syr'ia, 211, no. 2, pp Received September 19, 212 Revised November 25, 213 * Corresponding author.
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DOI: /jcprm
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