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1 DI: /jcprm :[ : ]. 1*,. 1,. 1,. 1,. 1,. 2,. 2,. 2 1,., 28,, ( ), punegov@ib.komisc.ru 2,., 48,, ( ), chukicheva-iy@chemi.komisc.ru - ( ) Hypericum perforatum., 55%-, 40, - 0, : Hypericum perforatum,,,, -,,. «-», «- (,, ) - : -»,, ,, punegov@ib.komisc.ru, kostromin@ib.komisc.ru -,,, zainullin@ib.komisc.ru,, ushkova@ib.komisc.ru,,, belykh-dv@mail.ru,,, chukicheva-iy@chemi.komisc.ru,,.: (8212) , : (8212) ( ) ( -,.) - - ( ) (Hypericum perforatum L). - microwave assisted extraction (MAE) [1].. *,.
2 126.,.,.. (1) (2). 1).,, LG MS2322T a 7 6a a 7 6a 6 5 H 3 C a 7b 14d 14e 3b 4 H a 7b 14d 14e 3b 4 H 3 C b 13b 14c 14b 3a 3 H 3 C b 13b 14c 14b 3a a 14 14a a 14 14a 1 2 (1) (2). 1. (1) (2) Hypericum perforatum L.. Hypericaceae Juss.) -., - 0,03 0,08% 0,1% [2], -, -., Sigma Aldrich 10 (95%) 298,5 [3], 5 (95%) 466,5 [4]. -,,. H. perforatum,. 3,,,. H. perforatum L.,., 0,5. : ( -, ) ,0 H. perforatum 6% , -2/150- (, ) ( )
3 127, %. -, ,. - LG MS2322T 600 0, ) ,, % UV1700 Shimadzu Ind. Ink. ( ). [5] %., 20, 30, %, , 30, 60, 120, 3, 30, : +, %; :, %;,,. 3.. :, %; :, %;,,,
4 128.,., : +, %; : ( ). 55% ;,,,,. 5. (% ) H. perforatum ;,,, ; : 1 3, 2 30, , Abu- Samra. [6] -, -. (1986.), -,, [7] ,,,, -.., ).. [8], - 70%.,,,, -.,, -,. 2, 3. 2, % 0,04 0,08% % %
5 129-70% %. - 0,082 0,093%., -, -, 55%. ( )., 82,4 ( 80 ),,, -. 3, 20 55% - 0,025 0,075% % 0,075 0,037%,. -, 30,, -, % (0,08 0,081% ).,, - (0,086%),,, 55%. -,,, [1].,, -,. -. (. 4), ,, , (3 30 ),, 86,4% ( ), 83,6%.,, -. 3, 20, 1 : 100 [5], 60, 70% 1 : 20 [9]., -,, -
6 130.,., Mandal V., Mohan Y., Hemalatha S. Microwave assisted extraction An innovative and promising extraction tool for medicinal plant research // Pharmacognosy Reviews Vol. 1, N1. Pp ,.,.,. Hypericum perforatum (Hypericaceae) ( ) // , URL: 4. URL: 5..,.,.,.,. - Hypericum L. // , Abu-Samra A., Morris J.S., Koirtyohann S.R. Wet ashing of some biological samples in a microwave oven // Analytical Chemistry Vol. 47. Pp Ganzler K., Bati J., Valko K. A new method for the extraction and high- performance liquid chromatographic determination of vicine and convicine in fava beans. In. Proceedings of the 2nd International Eastern European-American Symposium of Chromatography // J. Chromatography Vol Pp ,.,.,. - Hypericum perforatum // ,. Hypericum perforatum L. //. -, Punegov V.V. 1*, Kostromin V.I. 1, Fomina M.G. 1, Zaynullin V.G. 1, Yushkova E.A. 1, Belyh D.V. 2, Chukicheva I.U. 2, Zaynullin G.G. 2 EXTRACTIN F HYPERICIN AND PSEUDYPERICIN F HYPERICUM PERFRATUM IN MI- CRWAVE ACTIVATIN PRCESS 1 Institute of Biology, Komi Scientific Center, Ural Branch of the Russian Academy of Sciences, Kommunisticheskaia st., 28, Syktyvkar, (Russia), punegov@ib.komisc.ru 2 Institute of Chemistry, Komi Scientific Center, Ural Branch of the Russian Academy of Sciences, Pervomaiskaia st., 48, Syktyvkar, (Russia) The influence of the main parameters for extraction with microwave activation pigments (hypericin and pseudohypericin) of raw phytomass Hypericum perforatum were studied here. It was found that the maximal extraction of the pigments is achieved using 55% ethanol or isopropanol as extractants and liquor equal to 40 (power density of microwave radiation at a frequency of 0,0205 W/cm MHz and duration of microwave 60 seconds). It was experimentally proved that the method of extracting the microwave activation of hypericin and pseudohypericin of Hypericum perforatum is a particular example of the intensification of mass transfer in a system of capillary porous body-liquid. Found that microwave activation method allows extraction of ten times to reduce the time necessary to fully extract of Hypericum perforatum pigments compared with the classical methods of extraction. Keywords: Hypericum perforatum, hypericin, pseudohypericin, microwave assisted extraction, water duty, the composition of the extractant, spectrophotometry References 1. Mandal V., Mohan Y., Hemalatha S. Pharmacognosy Reviews, 2007, vol. 1, no. 1, pp Portniagina N.V., Echishvili E.E., Punegov V.V., Mishurov V.P. Rastitel'nye resursy, 2009, vol. 45, no. 2, pp (in Russ.) Belikov V.V., Tochkova T.V., Shatunova L.V., Kolesnik N.T., Baiandina I.I. Rastitel'nye resursy, 1990, vol. 26, no. 4, pp (in Russ.). 6. Abu-Samra A., Morris J.S., Koirtyohann S.R. Analytical Chemistry, 1975, vol. 47, pp Ganzler K., Bati J., Valko K. J. Chromatography, 1986, vol. 371, pp Agabalaev A.A., Karankevich E.G., Popova.P., Kuvaeva Z.I. Izvestiia NAN Belarusi, seriia Khimicheskikh nauk, 2011, no. 4, pp (in Russ.). 9. Sychev R.L., Punegov V.V. Novye dostizheniia v khimii i khimicheskoi tekhnologii rastitel'nogo syr'ia. [New advances in chemistry and chemical engineering plant materials.]. Barnaul, 2007, pp (in Russ.). * Corresponding author. Received March 21, 2013
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