Zebron MultiResidue GC Columns

Transcription

1 Zebron MultiResidue GC Columns A New Generation of Columns for Pesticide Analysis

2 MultiResidue Optimized for pesticide, herbicide and insecticide analysis The use of many pesticides has been limited or discontinued due to the health and environmental risks they pose. However, the persistence of these compounds in the environment has left many sites heavily contaminated and in need of remediation. There is also international concern for the residual level of pesticides in foods, especially those imported from countries where banned pesticides are still permitted. Though the standard method for pesticide testing varies throughout the world, many labs use Electron Capture Detectors (ECD), Nitrogen Phosphorous Detectors (NPD) or Flame Photometric Detectors (FPD) because of the sensitivity level they offer for specific compound classes. While these detectors are extremely sensitive, they do not provide spectral confirmation of the analyte. In order to provide positive confirmation, Mass Spectrometry (MS) must be used. The GC/MS can be used in Selected Ion Monitoring (SIM) mode to provide sensitivity levels similar to the previously mentioned detectors, while also providing positive confirmation of the analyte in question. Regardless of the technique, the new MultiResidue GC columns have been optimized for pesticides, herbicide, or insecticide analysis. Each column has a unique selectivity that allows them to be used together in dual column confirmation analysis. Both phases are MS Certified, so they can also be used on MS to confirm results. We have run a number of pesticide compounds on these phases. If you don t find the application you are looking for in this brochure, be sure to contact your local Phenomenex representative.

3 Zebron MultiResidue (MR) Columns Optimized Selectivity Two Complementary Phase Chemistries MS Certified Bleed Levels High Temperature Limits (0/0 C) Very Low Column Activity Table of Contents Compound ass Official Method Detection Page Number Chlorinated Pesticides EPA 0A ECD,, 9, Multi-Pesticide Residue PDP MS Aroclors EPA 0 ECD Organophosphorous Pesticides EPA 0//A FID Chlorophenoxy Acid Herbicides EPA / ECD Polyaromatic Hydrocarbons EPA 0/00 MSD Polychlorinated Biphenyls (PCBs) DIN ECD Phenomenex international@phenomenex.com Web:

4 MultiResidue A New Generation of Columns for Pesticide Analysis Optimized Selectivity Many pesticide compounds have very similar structures or have isomers that are present in the product. Zebron MultiResidue phases were developed to provide optimum selectivity for a variety of pesticide compounds. Every analysis is unique, but we can help you determine the best phase to use in order to resolve your pesticide sample. Figure : Selectivity of DDT, DDD, and DDE isomers at ng on column using MS Sample:. o,p-dde. p,p-dde. o,p-ddd. o,p-ddt. p,p-ddd. p,p-ddt min App ID: Abundance Low Bleed No 0 bleed ions at 0 C Acquisition Spectra Low Bleed Zebron columns have developed a reputation for low column bleed and high temperature limits and Zebron MultiResidue columns are no different. The columns are made using an extremely stable siloxanebased polymer that contains absolutely no cyano functionality. Our Engineered Self Crosslinking (ESC) bonding process makes both columns MS certified, allowing for unmatched spectral integrity even for low-level samples. Abundance NIST Reference Spectra Mass/Charge (m/z) 99 Mass/Charge (m/z)

5 Low Column Activity Many pesticides are sensitive to system activity and readily breakdown. This can be a significant problem when working with low level samples and sensitive detectors such as ECD. Zebron MultiResidue columns have been completely deactivated and provide excellent peak shape for even the most active compounds. Figure : Breakdown for sensitive pesticides on Zebron MultiResidue columns Endrin Breakdown. % Degradation Products App ID: Breakdown. % DDT Universal Compatibility Zebron MultiResidue GC columns are compatible with all systems and all detectors. It doesn t matter if it s an Agilent, Thermo Finnigan, Shimadzu, or Perkin Elmer GC or GC/MS system, Zebron columns provide reliable results every time. App ID: min min Tunable Selectivity Zebron MultiResidue GC columns were developed specifically for pesticide analysis. Their unique selectivity improves the separation of all classes of pesticides, herbicides, and insecticides. Since the columns are MS Certified, samples can be prescreened using analyte specific detectors, and then confirmed using MS. Sensitive and Stable Figure : Repeatability at pg on-column concentration of various pesticides using Zebron and MR- columns Response Response MR Sample Number Sample Number Table : Relative standard deviation (RSD) for five replicate injections of pesticide at pg on-column concentration Analyte % RSD MR- % RSD Lindane (γ-bhc).. Heptachlor..0 Dieldrin.. Endrin.. DDT.9. Methoxychlor..9 Phenomenex international@phenomenex.com Web:

6 MultiResidue Area Count Performance That Exceeds US EPA Method 0A Specifications The EPA outlines strict performance guidelines that must be met for compound linearity, percent relative standard deviation (% RSD), and breakdown of DDT and endrin. Column resolution and performance are critical in meeting these requirements. The data below was calculated using EPA Method 0A guidelines. Endrin R = DDT R = Parts Per Billion (ppb) Table : Five-point calibration curve at, 0,,00, and 0 ppb Analyte Zebron % RSD* Zebron MR- % RSD* US EPA Specifications a-bhc..9 < 0 γ-bhc (Lindane)..0 < 0 b-bhc. 9. < 0 d-bhc.90. < 0 Heptachlor.. < 0 Aldrin.. < 0 Heptachlor epoxide.0. < 0 g-chlordane.. < 0 a-chlordane.9.9 < 0 Endosulfan I.9.9 < 0 DDE.. < 0 Dieldrin.. < 0 Endrin.. < 0 DDD.9. < 0 Endosulfan II.. < 0 Endrin aldehyde.. < 0 DDT.0. < 0 Endosulfan sulfate..0 < 0 Methoxychlor.9. < 0 Endrin ketone..9 < 0 Average.. < 0 *Calculated using response factors as per EPA guidelines Area Count MR- Endrin R = DDT R = Parts Per Billion (ppb) Table : Percent breakdown of endrin & DDT as per EPA guidelines Analyte Zebron % Breakdown Zebron MR- % Breakdown US EPA Requirements Endrin..0 < DDT..9 <

7 Baseline Resolution of all 0 EPA 0A s Chlorinated Pesticides! The US EPA regulates the testing of 0 specific chlorinated pesticides under the official Method 0A. The method specifies an Electron Capture Detector (ECD), which is extremely sensitive for chlorinated compounds. However, it does not provide any confirmatory information about the peak. To reduce the occurrence of misidentifications, the method requires the use of two GC columns of dissimilar selectivity in a parallel configuration. The EPA considers an analyte s presence confirmed if it has a peak at the pre-determined retention time on both columns. The unique selectivity of Zebron MultiResidue columns allows for baseline resolution (R s >.) of all compounds with two elution order changes between the columns! App ID: Enhanced Performance Two elution order changes between the columns 0 min 9 0 MR- 9 0 App ID: US EPA 0A min Column: Zebron MultiResidue- Zebron MultiResidue- Dimensions: 0 meter x 0. mm x 0.0 µm 0 meter x 0. mm x 0. µm Part No.: HM-G0-; HM-G0- Injection: 0 C, µl Carrier Gas: ml/min (constant flow) Oven Program: 00 C for 0. min to 0 C/min to 0 C at 0 C/min for min Detector: 0 C Notes: Columns connected using a m Z-Guard and a Y-splitter Injector m Z-Guard Zebron Zebron MR- Y-Splitter ECD Detector ECD Detector Sample:. Tetrachloro-m-xylene (TCMX) (surr). -Bromo--nitrobenzene. a-bhc. g-bhc (Lindane). b-bhc. δ-bhc. Heptachlor. Aldrin 9. Heptachlor epoxide 0. g-chlordane (trans). a-chlordane (cis). Endosulfan I., -DDE. Dieldrin. Endrin., -DDD. Endosulfan II. Endrin aldehyde 9., -DDT 0. Endosulfan sulfate. Methoxychlor. Endrin ketone. Decachlorobiphenyl (DCB) (surr) Phenomenex international@phenomenex.com Web:

8 MultiResidue Increase Your Lab s Productivity Zebron MultiResidue columns were designed to provide optimized resolution of all classes of pesticides. This unique selectivity allows your lab to maintain separation of critical compounds even when analysis times are shortened by more than 0 %. The increase in lab productivity makes sure you get results to your customers on time, even for rush samples. Instruments Required to Run 00 Samples Per Day 0 Samples 0 Samples 0 Samples 0 Samples 0 Samples 0 Samples 0 Samples 9 0 Standard Conditions 90 App ID: 9 0 min MR App ID: 0 0 min Column: Zebron MultiResidue- Zebron MultiResidue- Dimensions: 0 meter x 0. mm x 0.0 μm 0 meter x 0. mm x 0.0 μm Part No.: HK-G0-; HK-G0- Injection: 0 C, µl Carrier Gas: ml/min (constant flow) Oven Program: 90 C for 0. min to 0 C/min for min Detector: 0 C

9 Same Columns + Optimized Conditions = Increased Productivity. Instruments Required to Run 00 Samples Per Day Samples Samples Samples Samples with time to spare! These instruments available for other analysis MR- Column: Zebron MultiResidue- Zebron MultiResidue- Dimensions: 0 meter x 0. mm x 0.0 μm 0 meter x 0. mm x 0.0 μm Part No.: HK-G0-; HK-G0- Optimized Conditions min App ID: App ID: min Sample for all columns on p. -9:. Tetrachloro-m-xylene (TCMX) (surr). -Bromo--nitrobenzene (IS). a-bhc. g-bhc (Lindane). b-bhc. d-bhc. Heptachlor. Aldrin 9. Heptachlor epoxide 0. trans-chlordane (gamma). cis-chlordane (alpha). Endosulfan I., -DDE. Dieldrin. Endrin. DDD. Endosulfan II. Endrin aldehyde 9. DDT 0. Endosulfan sulfate. Methoxychlor. Endrin ketone. Decachlorobiphenyl (DCB) (surr) Injection: 0 C, µl Carrier Gas: ml/min (constant flow) Oven Program: 0 C for 0. min to 0 0 C/min to 0 0 C/min for min Detector: 0 C % Shorter Analysis Almost no change in resolution! Tech Tip: High flow rates reduce a compound s residence time in the inlet. This can significantly reduce injection port breakdown for sensitive compounds such as Endrin & DDT. Phenomenex international@phenomenex.com Web:

10 MultiResidue Multi-Pesticide Residue Testing by GC/MS Pesticides are widely used by farmers to control pests, weeds and molds that would otherwise decrease crop production. While this has significantly increased worldwide food productions, these same pesticides pose significant health and environmental risks. The restrictions for specific pesticides differ from one country to the next. As world trade increases, the potential threat to other countries populations increases. This is especially true in the European Union, where produce can be transported from one country to another quite easily. For this reason, pesticides are the subject of increasing regulation. Since many different types of pesticides can be used on the same food product, multiple residue screening approaches are used to test for more than 00 compounds. Gas Chromatography (GC) is still the most common test method for the majority of pesticide classes. While ECD or NPD may be used for screening, MS detection must be employed to provide positive confirmation. Zebron MultiResidue columns are well suited for use on all types of detectors. They provide very low bleed levels even at elevated temperatures required to remove matrix contamination during high temperature bake outs. When used in conjunction with a screening method that uses an analyte specific detector such as ECD, Zebron and MR- columns can be a powerful tool in identifying positive samples. 0

11 Confirmation of Multi-Pesticide Mixture by GC/MS The low bleed performance of both Zebron MultiResidue and MR- columns allow them to be used on GC/MS. -, Retention Time Data , 9,9 9 Retention time data is available for over 00 pesticides on a Zebron MultiResidue- column. Please contact your local Phenomenex representative for more details. Column: Zebron MultiResidue- Dimensions: 0 meter x 0. mm x 0. μm Part No.: HG-G0- Injection: 0 C, µl Carrier Gas: 0.90 ml/min (constant flow) Oven Program: 0 C for 0. min to 0 0 C/min to 0 C/min to 0 C/min for min Detector: 0 C; -00 amu, , App ID: 0 0, min Sample:. Dichlorvos. DEET. EPTC.,-Dichlorobenzoic acid (methyl ester). Butylate. -Nitrophenol (methyl ester). Vernolate. Mevinphos 9. Mevinphos isomer 0. Pebulate. Trichlorfon. Dicamba (methyl ester). Molinate. Tebuthiuron. MCPP (methyl ester). Tetraethyl pyrophosphate (methyl ester). MCPA (methyl ester). Demeton isomer 9. Thionazin 0. Dichloroprop (methyl ester). Propachlor. Cycloate. Ethoprop. Trifluralin. Benefin.,-D (methyl ester). Sulfotep. Naled 9. Chlorpropham 0. Dicrotophos. Phorate. Monocrotophos. Pentachlorophenol (methyl ester). Demeton. Atraton. Profluralin. Prometon. Silvex (methyl ester) 9. Terbufos 0. Dimethoate. Simazine. Propazine. Atrazine. Diazinon. Dioxathion. Terbuthylazine. Fonofos. Pronamide 9. Chloramben (methyl ester) 0.,,-T Methyl ester. Phosphamidon isomer. Disulfoton. Secbumeton. Terbacil. Dinoseb (methyl ester). Dichlofenthion.,-DB (methyl ester). Phosphamidon 9. Chlorpyrifos methyl 0. Alachlor. Bentazon (methyl ester). Ronnel. Prometryn. Methyl parathion. Ametryn. Simetryn. Aspon. Metribuzin 9. Terbutryn 0. Malathion. Fenitrothion. Pichloram (methyl ester). Metolachlor. Chlorpyrifos. DCPA. Bromacil. Fenthion. Trichloronate 9. Triadimeton 0. Isopropalin. Parathion. MGK-. Merphos. Pendimethalin. Diphenamid. MGK- isomer. ofenvinfos. Crotoxyphos 9. Butachlor 90. Stirofos 9. Tokuthion 9. Napropamide 9. Fenamiphos 9. Merphos Oxide 9. Oxadiazon 9. Oxyflurofen 9. Carboxin 9. Tricyclazole 99. Acifluorfen 00. Ethion 0. Fensulfothion 0. Carbofenotion 0. Famfur 0. Norflurazon 0. Hexazinone 0. EPN 0. Phosmet 0. Leptophos 09. Azinphos-methyl 0. Fenarimol. Azinphos-ethyl. Coumaphos Phenomenex international@phenomenex.com Web:

12 MultiResidue EPA US Method EPA 0 Polychlorinated Biphenyls as Aroclors Aroclor App ID: MR- App ID: 0 0 min min Aroclor 0 MR- App ID: 9 App ID: min min Column: Zebron MultiResidue- Zebron MultiResidue- Dimensions: 0 meter x 0. mm x 0.0 µm 0 meter x 0. mm x 0. µm Part No.: HM-G0-; HM-G0- Injection: Split 0 C, µl Carrier Gas: Oven Program: ml/min (constant pressure) 0 C (hold min) to 00 9 C/min (hold 0 min) Detector: 0 C Sample:. Tetrachloro-m-xylene (TCMX) (surr). Decachlorobiphenyl (DCB) (surr) Notes: Columns connected using a meter Z-Guard and a Y-splitter How long will columns last? The performance of a GC system will degrade over time requiring inlet maintenance, column trimming, and ultimately the replacement of the GC column. In order to accurately determine what your column lifetime will be it s better to ask yourself the question: What makes your columns fail? Is it bleed, activity, or poor resolution? The better the column performance for that criteria initially will usually lead to better overall performance in the long term. Zebron MultiResidue columns provide the lowest activity, best resolution, and overall most stable performance of any pair of columns designed specifically for pesticide testing.

13 US EPA 0A RCP/MCP States of Connecticut and Massachusetts Pesticide List Column: Zebron MultiResidue- Zebron MultiResidue- Dimensions: 0 meter x 0. mm x 0.0 µm 0 meter x 0. mm x 0.0 µm Part No.: HK-G0-; HK-G0- Injection: 0 C, µl Carrier Gas: psi (constant pressure) Oven Program: 0 C for min to C/min (hold min) Detector: 0 C App ID: 0 min MR-, App ID: 9 0 min Sample:. Hexachlorocyclopentadiene. Propaclor. Tetrachloro-m-xylene (TCMX) (surr). a-bhc. Hexachlorobenzene. g-bhc (Lindane). b-bhc. Alachlor 9. d-bhc 0. Heptachlor. Aldrin. Heptachlor epoxide. g-chlordane. a-chlordane. Endosulfan I., -DDE. Dieldrin. Endrin 9., -DDD 0. Endosulfan II. Endrin aldehyde., -DDT. Endosulfan sulfate. Methoxychlor. Endrin ketone. Decachlorobiphenyl (DCB) (surr) This application was supplied courtesy of Keith Aloisa at Phoenix Labs. Phoenix Labs ( is a full service environmental lab that is NELAC accredited and certified in all of New England, New York, and New Jersey. Better resolution. Higher efficiency. Best results I ve ever gotten with any column pair. Do you have a noteworthy application using Zebron GC columns that you would like published? For consideration, please send your chromatogram and a short abstract to skyc@phenomenex.com or fax it to (0) - attn: Sky Countryman. Any type of application is welcome. Phenomenex international@phenomenex.com Web:

14 MultiResidue EPA US Method EPA 9 Organophosphorous Pesticides 0, App ID: 0 0 min, MR ,, App ID: 0 0 min Column: Zebron MultiResidue- Zebron MultiResidue- Dimensions: 0 meter x 0. mm x 0.0 µm 0 meter x 0. mm x 0. µm Part No.: HM-G0-; HM-G0- Injection: 0 C, µl Carrier Gas: ml/min (constant flow) Oven Program: 00 C for 0. min to 0 0 C/min to 0 C/min to 0 C/min for min Detector: 0 C Notes: Analytes at ppm in dichloromethane. Columns connected using a m Z-Guard and a Y-splitter Sample:. Dichlorvos. Mevinphos. Trichlorfon. TEPP (Tetraethyl Pyrophosphate). Demeton isomer. Thionazin. Ethoprop. Sulfotep 9. Naled 0. Dicrotophos. Phorate. Monocrotophos. Demeton. Terbuphos. Diazinon. Dimethoate. Fonofos. Phosphamidon isomer 9. Disulfoton 0. Dichlofenthion. Phosphamidon. Chlorpyrifos methyl. Ronnel. Aspon. Methyl parathion. Malathion. Fenitrothion. Chlorpyrifos 9. Fenthion 0. Trichloronate. Parathion. Merphos. Chlorfenvinphos. Crotoxyphos. Stirofos. Tokuthion. Merphos oxide (tribufos). Ethion 9. Fensulfothion 0. Contaminant. Carbofenthion. Famfur. EPN. Phosmet. Leptophos. Azinphos methyl. Azinphos ethyl. Couphomos

15 EPA US Method EPA / Chlorophenoxy Acid Herbicides min App ID: Request Additional EPA Methods 0 Aroclors 0 Nitrogen- and Phosphorus- Containing Pesticides 0A Chlorinated Pesticides MR App ID: 0 min Column: Zebron MultiResidue- Zebron MultiResidue- Dimensions: 0 meter x 0. mm x 0.0 μm 0 meter x 0. mm x 0. μm Part No.: HM-G0-; HM-G0- Injection: 0 C, µl Carrier Gas: ml/min (constant flow) Oven Program: 0 C for min to 0 C/min for min to 0 C/min to 0 0 C/min Detector: 0 C Sample:. Dalapon.,-Dichlorobenzoic acid. -Nitrophenol. DCAA (surr). Dicamba. MCPP. MCPA. Dichloroprop 9. Contaminant 0.,-D. Pentachlorophenol. DBOB (IS). Silvex. Chloramben.,,-T. Dinoseb.,-DB. Bentazon 9. Picloram 0. DCPA. Acifluorfen

16 MultiResidue EPA US Method EPA 0/ Polyaromatic Hydrocarbons,,,, App ID: 0 0 min Tech Tip: Column: Zebron MultiResidue- Dimensions: 0 meter x 0. mm x 0. µm Part No.: HG-G0- Injection: 00 C, µl Carrier Gas: ml/min (constant flow) Oven Program: 0 C for 0. min to C/min to 0 C/min for min Detector: 0 C; -00 Sample:. Naphthalene. -Methylnaphthalene. Acenaphthylene. Acenaphthene. Fluorene. Phenanthrene. Anthracene. Fluoranthene 9. Pyrene 0. Benzo[c]fluorene. Benz[a]anthracene. Cyclopenta[c,d]pyrene. Chrysene. Methylchrysene. Benzo[b]fluoranthene. Benzo[j]fluoranthene. Benzo[k]fluoranthene. Benzo[a]pyrene 9. -Methylcholanthrene 0. Dibenz[a,h]acridine. Dibenz[a,j]acridine. Indeno[,,-cd]pyrene. Dibenz[a,h]anthracene. Benzo[g,h,i]perylene. H-Dibenzo[c,g]carbazole. Dibenzo[a,l]pyrene. Dibenzo[a,e]pyrene. Dibenzo[a,i]pyrene 9. Dibenzo[a,h]pyrene Inlet deactivation is critical for obtaining stable calibration curves. Use a liner style that has a taper at the bottom to help focus analytes onto the column. Avoid liners with glass wool because it adds activity. Also remember to change your gold seal regularly when working with Agilent 90 or 90 instruments.

17 DIN Method : Polychlorinated Biphenyls Separation Resolving Critical Isomers Polychlorinated Biphenyls (PCBs) are a class of priority environmental pollutants that have been identified for international regulation. The similarity in structure and polarity makes the resolution of certain isomers challenging on standard phases. German law requires separation of specific PCB congeners under DIN Method. The unique selectivity offered by Zebron MulitResidue columns makes them a versatile solution for many applications outside of pesticide analysis. Both the and the MR- columns provide resolution of all the required PCB congeners in less than 0 minutes, allowing for simultaneous confirmation of each sample App ID: 0 0 min Sample: MR-. Tetrachloro-m-xylene (TCMX) (surr). BZ#. BZ#. BZ#. BZ# 0. BZ#. BZ#. BZ# 9. BZ# 0. BZ#. BZ# 0 Note: BZ = Ballschmiter. BZ#. BZ#. BZ#. BZ#. BZ#. BZ# 0. BZ# 0 9. BZ# 9 0. BZ# 9. Decachlorobiphenyl (DCB) 0 9, 9 0 App ID: 0 0 min Column: Zebron MultiResidue- Zebron MultiResidue- Dimensions: 0 meter x 0. mm x 0.0 μm 0 meter x 0. mm x 0. μm Part No.: HM-G0-; HM-G0- Injection: 0 C, µl Carrier Gas: ml/min (constant flow) Oven Program: 00 C for 0. min to 00 C/min to 0 C/min for min Detector: 0 C Phenomenex international@phenomenex.com Web:

18 MultiResidue Ordering Information Choose the Best Column Set for Your Needs We know every lab is different and we want to help make choosing the right GC column easy. Zebron MultiResidue columns are available in a variety of formats to meet your lab s needs. 0. mm ID Provides the overall best resolution, lowest bleed, and greatest sensitivity. Recommended when using GC/MS or when needing the lowest detection levels possible. Does not handle highly contaminated samples well, column lifetime might be reduced. Resolution Stability Sensitivity Bleed Low High 0. mm ID Good compromise between resolution and column stability. Recommended when working with dual column setups where high level samples will be encountered. Works well on newer MS systems. High flow rates decrease residence time in the inlet. Low Resolution Stability Sensitivity Bleed High 0. mm ID Recommended when working with highly contaminated samples. Good for screening instruments. Resolution and sensitivity will be slightly reduced when compared to 0. or 0. mm ID columns. However, Zebron MultiResidue phases have very high selectivity for pesticides. Thus, resolution will be greater than other phases recommended for this work. Low Resolution Stability Sensitivity Bleed High Column Installs This End Phenomenex Recommended Liners Description Split / Splitless Liner with wool Splitless Liner Splitless Liner Single Taper Direct Connect with Side Hole (top) Single Taper Direct Connect with Side Hole (bottom) GC Instruments & Model No. Agilent 0/90/90 Shimadzu A, B, 00 Varian 0/0 Agilent 0/90/90 Agilent 0/90/90 Dimensions ID x L x OD (mm) Part No. Unit Price x. x. AG0- /pk. x 9. x.0 AG0- /pk x x. AG0- /pk x. x. AG0-0 /pk x. x. AG0- /pk

19 Zebron MultiResidue Zebron MultiResidue ID (mm) Temp. Limits C df (µm) Part No. df (µm) Part No. Price 0-Meter to 0/0 0. HG-G0-0.0 HG-G to 0/0 0.0 HM-G0-0. HM-G to 0/0 0.0 HK-G0-0.0 HK-G0- Zebron MultiResidue Column Kits 0. mm ID Kit KG0- Description Dimension Part No. Price MultiResidue- 0 meter x 0. mm x 0. µm df HG-G0- MultiResidue- 0 meter x 0. mm x 0.0 µm df HG-G0-0 Z-Guard meters x 0. mm AG-G GZ0 Universal Capillary Column Y-connector, Borosilicate AG0-0. mm ID Kit KG0- Description Dimension Part No. Price MultiResidue- 0 meter x 0. mm x 0.0 µm df HM-G0- MultiResidue- 0 meter x 0. mm x 0. µm df HM-G0- Z-Guard meters x 0. mm AM-G GZ0 Universal Capillary Column Y-connector, Borosilicate AG0-0. mm ID Kit KG0-9 Description Dimension Part No. Price MultiResidue- 0 meter x 0. mm x 0.0 µm df HK-G0- MultiResidue- 0 meter x 0. mm x 0.0 µm df HK-G0- Z-Guard meter x 0. mm AK-G GZ0 Universal Capillary Column Y-connector, Borosilicate AG0- Gold Inlet Base Seals Standard, Single Groove for Splitless Applications, 0. mm dia. Inlet Hole Part No. Description Similar to Mfr. No.* Unit Price AG0- AG0-9 Gold Inlet Base Seal, splitless (single groove) Gold Inlet Base Seal, splitless (single groove) Replacement Inlet Seal Washers 0-0 /pk 0-0 0/pk Part No. Description Similar to Mfr. No.* Unit Price AG0- Inlet Seal Washers, for Agilent GC injection port Inlet seal washers are sold separately from inlet base seals. * Similar to but not always an exact equivalent to the original manufacturer s product. 0-9 /pk Phenomenex international@phenomenex.com Web: 9

20 Phenomenex: Years of Progress Since its founding in 9, Phenomenex has been dedicated to the development, manufacturing and supply of products and accessories for the separation, analysis and purification of chemicals and biochemicals. Offering the widest selection of chromatography columns and consumables from a single supplier worldwide, Phenomenex s objective is to provide the very best product for every application. The company has subsidiaries and leading market positions in the USA, Canada, United Kingdom, Germany, New Zealand, Australia, France, Ireland, Denmark, Italy, and many other countries. Our vast network of well-trained partners and distributors enables Phenomenex to successfully supply and support the work of scientists in more than 0 countries around the world. Zebron GC Columns In 99, Phenomenex diversified its business into the field of Gas Chromatography. It brought together chemists with more than years of GC column manufacturing knowledge from other major names in the GC column industry. The goal was simple: make a better GC column. Phenomenex has rapidly grown to be one of the leaders in GC column technology and already produces a significant portion of the high performance capillary columns used worldwide. The Best Guarantee in the Industry If Zebron does not provide you with better separation as compared to any other GC column of the same phase and comparable dimensions, send in your comparative data within days and keep your Zebron column for FREE! Zebron, MultiResidue and Engineered Self Cross-linking are trademarks of Phnomenex, Inc. Agilent is a registered trademark of Hewlett Packard. Thermo is a registered trademark of Thermo Scientific. Finnigan is a trademark of Thermo Scientfic. Phenomenex is in no way affiliated with Agilent, Hewlett Packard, or Thermo Scientific. The opinions stated herein are solely those of the author and not necessarily those of any company or organization. Shimadzu is a registered trademark of Shimadzu Instruments, Inc. Perkin Elmer is a registered trademark of Perkin-Elmer Corp. Varian is a trademark of Varian Analytical Instruments. 00 Phenomenex, Inc. All rights reserved. Phenomenex products are available worldwide. For the distributor in your country, contact Phenomenex USA, International Department by telephone, fax or international@phenomenex.com. mail: tel.: fax: Australia PO Box 0 Lane Cove, NSW 0 Australia info@ phenomenex.com.au Austria Zeppelinstr. Aschaffenburg Germany anfrage@ phenomenex.com Canada Madrid Ave. Torrance, CA USA (00) - (0) - info@ phenomenex.com Denmark Gydevang 9-0 Allerød Denmark 0 0 dkinfo@ phenomenex.com mail: tel.: fax: France Parc des Grillons, Bat. 0 route de Sartrouville Le Pecq Cedex France franceinfo@ phenomenex.com Germany Zeppelinstr. Aschaffenburg Germany anfrage@ phenomenex.com Ireland Queens Avenue, Hurdsfield Ind. Est., Macclesfield, Cheshire SK0 BN, UK eireinfo@ phenomenex.com Italy Via Emilia, /C 00 Anzola Emillia (BO) Italy italiainfo@ phenomenex.com 9_I mail: tel.: fax: New Zealand P O Box -0 Milford 0 North Shore City New Zealand info@ phenomenex.co.nz Puerto Rico Sierra Morena, Suite #0 San Juan, Puerto Rico 009 (00) -HPLC (0) - info@ phenomenex.com United Kingdom Queens Avenue, Hurdsfield Ind. Est., Macclesfield, Cheshire SK0 BN, UK ukinfo@ phenomenex.com USA Madrid Ave. Torrance, CA USA (0) -0 (0) - info@ phenomenex.com _U