GOLD NANOSTRACTURES: PROPERTIES AND MEDICAL APLICATIONS IUMS

Transcription

1 GOLD NANOSTRACTURES: PROPERTIES AND MEDICAL APLICATIONS IUMS Seyed Mohammad Amini

2 Going down to Nano-Regime

3 Introduction The Lycurgus cup is green, but glows red when a light is placed inside due to the gold nanoparticle content (Chemistry World 2010) Gold nanoparticles in medieval stained glass windows (Chemistry World 2010)

4 Introduction Chem. Soc. Rev., 2012 Gold nanostructures morphology

5 Gold Nanostractures Properties Gold is yellow diamagnetic noble metal with high atomic number Gold nanostracture properties Surface plasmon resonance (Amini et al. IET Nanobiotechnolgy) Surface enhanced raman spectroscopy (Stiles et al. Annual Review of Analytical Chemistry) Unique super atom Magnetisem, fluorescence (McCoy et al. ACS Nano, ) Catalectic activity in nanoscale (Zhou et al. Nature) Electric and thermal conductivity (Amini et al. Accepted in Gold Bulletin, Zhang et al. SPIE Proceedings) Biocompatibility (Murphy et al. Accounts of chemical research)) Antioxidant capacity (Shabani et al. nanomedicine) Controllable surface chemistry

6 Surface plasmon resonance Eustis & El seyed. Chemical society Review Yasun et al. Interface focus C ext 24 R 2 3 3/ 2 " m ' 2 "2 2 m

7 Proximity effect of GNPs SPR Chem. Soc. Rev., 2012

8 Surface enhanced raman spectroscopy (SERS) Energy-level diagram showing the states involved in Raman spectra. Vikipedia Electromagnetic theory (enhancement in the electric field provided by the surface) Chemical theory (involves charge transfer between the chemisorbed species and the metal surface)

9 Unique super atom Magnetisem The quantum states of electrons in small symmetric metallic clusters are grouped into shells similar to the electronic shells in free atoms, leading to the conceptual basis for defining superatoms. Al 7 = the property is similar to germanium atoms. Al 13 = the property is similar to halogen atoms, more specifically, chlorine. M@Au24(SR)18 cluster (McCoy et al. ACS Nano)

10 Unique super atom fluorescence Excitation (dashed) and emission (solid) spectra of different gold nanoclusters. Phys. Rev. Lett

11 Gold Nanoparticles Catalectic Activity Laoufi et al. Phsycal Chem C Gold nanoparticle catalysts are being developed for CO oxidation in gas masks (Chemistry World 2010)

12 Electric Conductivity of Gold Nanostractures Room temperature measurements of the photocurrent through films of pure and Pt-decorated CdSe NPs under laser illumination (λ = 637 nm) Meyns et al. ACS Nano Abdellahim et al. International Journal of the Physical Sciences y Variation of the residual resistivity and the interfacial volume fraction with the average grain size for the bulk Gd metals. Hong et al. Progress

13 Thermal Conductivity of Gold Nanostractures High Thermal Conductivity (In comparison with bulk gold( Photo thermal Effect Photo acoustic imaging Sintering Melting point vs. particle diameter of gold nanoparticles. Cheng et al. J Mat Chem C

14 Controllable surface chemistry Gold thiolate bond Passive adsorption N-Br-Au Binding sterategy Claire et al. RSC 2010

15 Gold Nanostracture Biocompatibility Gold nanoparticles nanoparticles are not cytotoxic and do not elicit secretion of proinflammatory cytokines Sastry et all. Langmuir 2005, 21, Effective factors: Morphological Effects (Size, Shape) Surface Chemistry

16 Gold Nanostracture Medical Application Radiosensitization X-ray contrast agent Photothermal Therapy Photodynamic Theapy Drug & gene delivery Electric field hyperthemia Biosensors, Bioelectronic, Fluorescence quenching Probes Two-photon microscopy, Acousto optic Imaging Surface-enhanced Raman spectroscopy, Near IR spectoscopy Production of antibodies by using GNPs, Adjuvant antibody therapy..

17 Radiosensitization Gold is the best nanoparticle High Z Metal It`s Biocompatible It`s a Carrier Easy chemistry Kwatra et al.

18 Phantom images of samples containing differents gold concentrations ( Exp ) measured by SRCT ( Meas ). The last phantom contains Au@DTDTPA- Gd 50 nanoparticles. b) In vivo measurement of gold concentration ([Au] meas ) in the bladder of a rat by SRCT (blue line). X ray Imaging Size Conc. Shape

19 Photoacoustic imaging Photoacoustic imaging is a biomedical imaging modality based on the photoacoustic effect.

20 Gold Nanostructures & Photothermal Effect Chem. Soc. Rev., 2012

21 23 RF-EF Heating of gold nanostractures Heating Mechanism Electrophoretic movement of particles cause the heating. Joule heating of gold particles. Joule heating of ionic backgrounds. Main Affecting Factors Particles properties( Size, concentration, material and so on), ionic backgrounds, EF frequency and power.

22 T ( o C) Radiofrequency Electric Field Hyperthermia 4 DI Water PEG-GNR 100 ppm (24 h dialysis) PEG-GNP 100 ppm (24 h dialysis) e Amini et al. Art. Cell Time (s) Amini et al. Gold Bulletin, Amini et al. Art Cell

23 PhotoDynamic Therapy 100 a b Absorbtion (a. u) GNP GNP+ALA (0.5mM) GNP+ALA (1mM) 100 GNP+ALA (1mM) GNP 100 d GNP GNP+ALA (0.5mM) GNP+ALA (1mM) Cell Viability(%) Cell Viability(%) Cell Viability (%) Cell Viability(%) Control Light ALA 0.5mM ALA 1mM Amini et al. IET Nanobiotechnoligy 0 Control nm GNP Concentration(g/ml) 0 Control nm GNP Concentration (g/ml) 0 Control nm GNP concentration(g/ml) MB 40 M GNP 125 ppm 1 min 5 min 10 min 20 min Wavelenghts (nm) Darabpoor et al. Dug delivery System and Technology

24 SERS & Gold Nanoparticles SERS Spectrum of different nucleotides on gold nanoparticles (unpublished data). Strobbia et al. SPIE 2015

25 Active or Passive Drug delivery A schematic illustration of drug delivery via active and passive targeting, solid and dotted line respectively

26 Transfection Application Schematic illustration of the monolayer protected gold nanoparticles used as transfection vectors

27 Photothermal effect of gold nanoparticles for releasing encapsulated materials a) Preparation of a microcapsule by layer-by-layer process, b) Encapsulation of therapeutic agents, c) Doping of GNPs in the capsule shells, d) Rupture of the shell upon light irradiation

28 Gold Clusters for Florescence Imaging There is no quenching with gold nanoclusters Wang et al. Scientific Reports, 2013

29 Gold nanoparticles in Biosesor Point of Care sensors FRET Sensors Electrochemical sensors SPR sensors

30 Point of Care Sencors Point of care sensors used different strategies It can be SPR or electrochemical or enzyme based sensor Easy to use It`s a rapid test Biosensors & Bioelectronics, 2016

31 Fluorescence resonance energy transfer (FRET) Gold nanoparticles act as fluorescence quencher Engendering the distance between gold particle and fluorescence probe is critical factor Shi et al. Biosensors and Bioelectronics 2014

32 LSPR sensors Mostly based on aggregation or de-aggregation of Gold nanostructures SPR Sensors LSPR Sensors Nature Photonics, 2012 Chemical Society Reviews, Issue 7, 2012

33 LSPR Sensor for Urinary Nuclear Matrix Protein 22 (NMP22) Fatemi et al. Colloid and Surface B: Biointerface

34 a b a b Fatemi et al. Colloid and Surface B: Biointerface

35 GNPs Conjugated Secondary Antibody in an ELISA Kit Model Emami et al. MONOCLONAL ANTIBODIES IN IMMUNODIAGNOSIS AND IMMUNOTHERAPY

36 Coleuge Dr. Sharmin Kharrazi Prof. Mahmoudreza Jaafari Dr. Zohre Jomefarsangi Saeed moayedi Elnaz Shabani Mozafar Imanizadeh Farnaz Fatemi Sima Mohammadi Tara Emami Khademi Smaeel Darabpoor Ali Karimi