Methods in Enzymology. Volume 102. Hormone Action. Part G Calmodulin and Calcium-Binding Protein s. Anthony R. Means. Bert W.
|
|
- Matthew Bryan
- 6 years ago
- Views:
Transcription
1 Methods in Enzymology Volume 102 Hormone Action Part G Calmodulin and Calcium-Binding Protein s Anthony R. Means Bert W. O'Malley
2
3 CONTRIBUTORS TO VOLUME 102 PREFACE VOLUMES IN SERIES ix xiii XV 1. Calmodulin Purification and Fluorescent Labeling JOHN R. DEDMAN AN D MARCIA A. KAETZEL 1 2. Purification of Plant Calmodulin JAMES MICHAEL ANDERSON 9 3. Purification of Calmodulin by Ca z+ -Dependent Af- HARRY CHARBONNEAU, finity Chromatography RITA RICE, RUSSELL C. HART, AND MILTON J. CORMIER Assay of Calmodulin by Cat+-Dependent Phos- ROBERT W. WALLACE, phodiesterase E. ANN TALLANT, AN D WAI YIU CHEUNG The Red Blood Cell as a Model for Calmodulin- THOMAS R. HINDS AN D Dependent Ca z+ Transport FRANK F. VINCENZI Myosin Light Chain Phosphorylation in Smooth PAUL J. SILVER AND Muscle and Nonmuscle Cells as a Probe of JAMES T. STULL 62 Calmodulin Functio n 7. Assessment of Caz+-Calmodulin Formation in In- DAVID R. MANNING AN D tact Vertebrate Skeletal Muscle JAMES T. STULL Spectroscopic Analyses of Calmodulin and Its In- RACHEL E. KLEVIT 82 teraction s 9. Production of Polyclonal and Monoclonal Anti- JAMES G. CHAFOULEAS, bodies to Calmodulin and Utilization of These MARY E. RISER, Immunological Probes LISETTE LAGAC@, AND ANTHONY R. MEANS Localization of Calmodulin and Calmodulin Ac- MICHAEL J. WELSH 11 0 ceptor Sites by Fluorescence Method s 11. Immunofluorescence Localization of Calmodulin JEFFREY F. HARPER AN D in Unfixed Frozen Tissue Sections ALTON L. STEINER Caz+ Binding to Calmodulin JAMES D. POTTER, PRISCILLA STRANG-BROWN, PATRICIA L. WALKER, AN D SHOZO IIDA Preparation of Calmodulin Crystals WILLIAM J. COOK AN D JOHN S. SACK 143
4 14. Preparation of Fluorescent Labeled Calmodulins BRADLEY B. OLwIN AND DANIEL R. STORM Posttranslational Modification of Calmodulin TIMOTHY J. MURTAUGH, PAUL M. RowE, PAMELA L. VINCENT, LYNDA S. WRIGHT, AN D FRANK L. SIEGEL Techniques for Measuring the Interaction of BENJAMIN WEISS 17 1 Drugs with Calmoduli n 17. Naphthalenesulfonamides as Calmodulin Antago- HIROYOSHI HIDAKA AN D nists TOSHIO TANAKA Synthesis and Characterization of Calmodulin An- RussELL C. HART, tagonistic Drugs MICHAEL D. BATES, MILTON J. CORMIER, GERALD M. ROSEN, AN D P. MICHAEL CONN Detection of Calmodulin-Binding Polypeptides JOHN R. GLENNEY, JR. AN D Separated in SDS-Polyacrylamide Gels by a KLAUS WEBER 204 Sensitive [ 1251]Calmodulin Gel Overlay Assay 20. Use of Calmodulin Affinity Chromatography for RAJENDRA K. SHARMA, Purification of Specific Calmodulin-Dependent WILLIAM A. TAYLOR, AND Enzymes JERRY H. WANG Calmodulin as an Integral Subunit of Phosphory- COLIN PICTON, lase Kinase from Rabbit Skeletal Muscle SHIRISH SHENOLIKAR, ROGER GRAND, AN D PHILIP COHEN Isolation and Characterization of Bovine Brain C. B. KLEE, Calcineurin : A Calmodulin-Stimulated Protein M. H. KRINKS, Phosphatase A. S. MANALAN, P. COHEN, AND A. A. STEWART Purification and Radioimmunoassay of Calmodu- E. ANN TALLANT, lin-dependent Protein Phosphatase from Bo- ROBERT W. WALLACE, AN D vine Brain WAI YIU CHEUNG Assay of S-100 Protein by an Enzyme Immuno- HIROYOSHI HIDAKA, assay Method TOYOSHI ENDO, AN D KANEFUSA KATO Covalent Regulation of the Cardiac Sarcoplasmic CHRISTIAN J. LE PEUCH, Reticulum Calcium Pump : Purification and DANIELLE A.-M. LE PEUCH, Properties of Phospholamban, a Substrate of AND JACQUES G. D LE 26 1 camp-dependent Protein Kinase and Ca t+ - Calmodulin-Dependent Phospholamban Kinase
5 26. Cat+-Dependent Neutral Protease and Proteolytic NoRIO KAJIKAWA, Activation of Cat+-Activated, Phospholipid-De- AKIRA KISHIMOTO, pendent Protein Kinase MAKOTO SHIOTA, AN D YASUTOMI NISHIZUKA Enzyme-Linked Immunoabsorbent Assay BARBARA E. MILLER AN D (ELISA) and Radioimmunoassay (RIA) for the ANTHONY W. NORMAN 29 1 Vitamin D-Dependent 28,000 Dalton Calcium - Binding Protei n 28. Chemical Approaches to the Calmodulin System THOMAS C. VANAMAN 296 AUTHOR INDEX 31 1 SUBJECT INDEX 321
Activation of Brain Calcineurin Phosphatase towards Nonprotein Phosphoesters by Ca2+, Calmodulin, and Mg2+*
THE JOURNAL OF BIOLOGICAL CHEMI3TRY 0 1984 by The American Society of Biological Chemists, Inc. Vel. 259, No. 14, lasue of July 25, pp. 8801-8807 1984 Printed in ~ s.a. Activation of Brain Calcineurin
More informationThe Practice of Medicinal Chemistry
A The Practice of Medicinal Chemistry Edited by CAMILLE G. WERMUTH Laboratoire de Pharmacochimie Moleculaire, Faculte de Pharmacie, Universite Louis Pasteur, Illkirch, France ACADEMIC PRESS Harcourt Brace
More informationMethods in Enzymology. Volume 156. Biomembranes. Part P ATP-Driven Pumps and Related Transport : The Na,K-Pump. Sidney Fleischer Becca Fleischer
Methods in Enzymology Volume 156 Biomembranes Part P ATP-Driven Pumps and Related Transport : The Na,K-Pump Sidney Fleischer Becca Fleischer CONTRIBUTORS TO VOLUME 156 PREFACE VOLUMES IN SERIES ix Xii
More informationChapter 16. Cellular Movement: Motility and Contractility. Lectures by Kathleen Fitzpatrick Simon Fraser University Pearson Education, Inc.
Chapter 16 Cellular Movement: Motility and Contractility Lectures by Kathleen Fitzpatrick Simon Fraser University Two eukaryotic motility systems 1. Interactions between motor proteins and microtubules
More informationPHYSIOLOGY CHAPTER 9 MUSCLE TISSUE Fall 2016
PHYSIOLOGY CHAPTER 9 MUSCLE TISSUE Fall 2016 2 Chapter 9 Muscles and Muscle Tissue Overview of Muscle Tissue types of muscle: are all prefixes for muscle Contractility all muscles cells can Smooth & skeletal
More informationUNIT 6 PART 3 *REGULATION USING OPERONS* Hillis Textbook, CH 11
UNIT 6 PART 3 *REGULATION USING OPERONS* Hillis Textbook, CH 11 REVIEW: Signals that Start and Stop Transcription and Translation BUT, HOW DO CELLS CONTROL WHICH GENES ARE EXPRESSED AND WHEN? First of
More informationAccording to the diagram, which of the following is NOT true?
Instructions: Review Chapter 44 on muscular-skeletal systems and locomotion, and then complete the following Blackboard activity. This activity will introduce topics that will be covered in the next few
More informationC a h p a t p e t r e r 6 E z n y z m y e m s
Chapter 6 Enzymes 4. Examples of enzymatic reactions acid-base catalysis: give and take protons covalent catalysis: a transient covalent bond is formed between the enzyme and the substrate metal ion catalysis:
More informationRNA Synthesis and Processing
RNA Synthesis and Processing Introduction Regulation of gene expression allows cells to adapt to environmental changes and is responsible for the distinct activities of the differentiated cell types that
More informationIntroduction... Theory Influence of Excitation Pulse Shape...
1. Fluorescence Anisotropy: Theory and Applications Robert F. Steiner 1.1. 1.2. 1.3. 1.4. 1.5. 1.6. Introduction... Theory... 1.2.1. Meaning of Anisotropy... 1.2.2. Influence of Excitation Pulse Shape...
More informationSerine-7 but not serine-5 phosphorylation primes RNA polymerase II CTD for P-TEFb recognition
Supplementary Information to Serine-7 but not serine-5 phosphorylation primes RNA polymerase II CTD for P-TEFb recognition Nadine Czudnochowski 1,2, *, Christian A. Bösken 1, * & Matthias Geyer 1 1 Max-Planck-Institut
More informationMuscle tissue. Types. Functions. Cardiac, Smooth, and Skeletal
Types Cardiac, Smooth, and Skeletal Functions movements posture and body position Support soft tissues Guard openings body temperature nutrient reserves Muscle tissue Special Characteristics of Muscle
More informationACTIVE TRANSPORT AND GLUCOSE TRANSPORT. (Chapter 14 and 15, pp and pp )
ACTIVE TRANSPORT AND GLUCOSE TRANSPORT (Chapter 14 and 15, pp 140-143 and pp 146-151) Overview Active transport is the movement of molecules across a cell membrane in the direction against their concentration
More informationRichik N. Ghosh, Linnette Grove, and Oleg Lapets ASSAY and Drug Development Technologies 2004, 2:
1 3/1/2005 A Quantitative Cell-Based High-Content Screening Assay for the Epidermal Growth Factor Receptor-Specific Activation of Mitogen-Activated Protein Kinase Richik N. Ghosh, Linnette Grove, and Oleg
More informationMicroscopy, Optical Spectroscopy, and Macroscopic Techniques
Microscopy, Optical Spectroscopy, and Macroscopic Techniques Methods in Molecular Biology John M, Walker, SERIES EDITOR 22. Microscopy, Optical Spectroscopy, and Macroscopic Techniques, edited by Christopher
More informationCardiac cell-cell Communication Part 1 Alonso P. Moreno D.Sc. CVRTI, Cardiology
Bioengineering 6003 Cellular Electrophysiology and Biophysics Cardiac cell-cell Communication Part 1 Alonso P. Moreno D.Sc. CVRTI, Cardiology moreno@cvrti.utah.edu November 2010 poster Physiological Relevance
More informationChem Lecture 9 Pumps and Channels Part 1
Chem 45 - Lecture 9 Pumps and Channels Part 1 Question of the Day: What two factors about a molecule influence the change in its free energy as it moves across a membrane? Membrane proteins function as
More informationLecture 19 (10/30/17) Enzyme Regulation
Reading: Ch5; 164, 166-169 Problems: none Remember Today at 6:30 in PHO-206 is the first MB lecture & quiz NEXT Reading: Ch5; 158-169, 162-166, 169-174 Lecture 19 (10/30/17) Problems: Ch5 (text); 3,7,8,10
More informationChapter 4. strategies for protein quantitation Ⅱ
Proteomics Chapter 4. strategies for protein quantitation Ⅱ 1 Multiplexed proteomics Multiplexed proteomics is the use of fluorescent stains or probes with different excitation and emission spectra to
More informationEnzyme Kinetics and Mechanism
Methods in Enzymology Volume 87 Enzyme Kinetics and Mechanism Part C Intermediates, Stereochemistry, and Rate Studie s Daniel L. Purich CONTRIBUTO RS TO VOLUME 87........ ix PREFACE VOLUMES IN SERIES
More informationRegulation of gene expression. Premedical - Biology
Regulation of gene expression Premedical - Biology Regulation of gene expression in prokaryotic cell Operon units system of negative feedback positive and negative regulation in eukaryotic cell - at any
More informationc. doubling the volume of the assay by adding buffer (assume you are using a spectrophotometric assay)
FST 123 1st Midterm Examination May 1, 2012 Name Key 1. We have discussed enzyme kinetics under the limiting conditions of very high and very low substrate concentrations. Under each of these conditions
More informationCurrent Standings Mar 25, :14 PM Page 1 of 7
Teams, Hdcp Teams, Handicapped 1 Scratchers & Cappers #2 15 3,054 2 In Harmony 19 2,902 3 All Mixed Up 3 2,899 4 T.N.N.T 17 2,888 5 Oliver 23 2,887 6 In Harmony 2 20 2,883 7 Chucks Chicks 24 2,843 8 Scratchers
More informationFinal Results Corinium Enduro 2016 Permit No: 46719
16 Andrew Frost Expert 618.702 598.754 1217.46 0 0 0 0 0 0 0 0 0 0.00 1217.46 PREMIER 18 Hayden Maller Expert 662.645 630.054 1292.70 0 0 0 0 0 0 0 1 0 60.00 1352.70 Class Winner 15 Daniel Alldred Expert
More informationRegulation and signaling. Overview. Control of gene expression. Cells need to regulate the amounts of different proteins they express, depending on
Regulation and signaling Overview Cells need to regulate the amounts of different proteins they express, depending on cell development (skin vs liver cell) cell stage environmental conditions (food, temperature,
More informationFundamentals of Neurosciences. Smooth Muscle. Dr. Kumar Sambamurti 613-SEI; ;
Fundamentals of Neurosciences Smooth Muscle Dr. Kumar Sambamurti 613-SEI; 792-4315; sambak@musc.edu 1 Smooth Muscle Structure Cells much smaller than skeletal muscle (2-5µM diam, 100-400µM long) Single
More informationThree types of RNA polymerase in eukaryotic nuclei
Three types of RNA polymerase in eukaryotic nuclei Type Location RNA synthesized Effect of α-amanitin I Nucleolus Pre-rRNA for 18,.8 and 8S rrnas Insensitive II Nucleoplasm Pre-mRNA, some snrnas Sensitive
More informationStudy Guide 11 & 12 MULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question.
Study Guide 11 & 12 MULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question. 1) The receptors for a group of signaling molecules known as growth factors are
More informationPHARMACOLOGY G PROTEIN COUPLED RECEPTORS
PHARMACOLOGY G PROTEIN COUPLED RECEPTORS Edited by Richard R. Neubig Department of of Michigan Medical School Ann Arbor, Michigan, USA Serial Editor S. J. Enna Department of Molecular and Integrative Physiology
More informationOverview of Kinetics
Overview of Kinetics [P] t = ν = k[s] Velocity of reaction Conc. of reactant(s) Rate of reaction M/sec Rate constant sec -1, M -1 sec -1 1 st order reaction-rate depends on concentration of one reactant
More informationPart One: The Chemistry of Life
Part One: The Chemistry of Life Chemistry is the study of matter and its changes. Organisms obtain and use many chemicals The metabolism of organisms involves many chemical reactions To understand all
More informationModeling. EC-Coupling and Contraction
Bioeng 6460 Electrophysiology and Bioelectricity Modeling of EC-Coupling and Contraction Frank B. Sachse fs@cvrti.utah.edu Overview Quiz Excitation-Contraction Coupling Anatomy Cross Bridge Binding Coupling
More informationComputational Modeling of Protein Kinase A and Comparison with Nuclear Magnetic Resonance Data
Computational Modeling of Protein Kinase A and Comparison with Nuclear Magnetic Resonance Data ABSTRACT Keyword Lei Shi 1 Advisor: Gianluigi Veglia 1,2 Department of Chemistry 1, & Biochemistry, Molecular
More informationThe Caspase System: a potential role in muscle proteolysis and meat quality? Tim Parr
The Caspase System: a potential role in muscle proteolysis and meat quality? Tim Parr Caroline Kemp, Ron Bardsley,, Peter Buttery Division of Nutritional Sciences, School of Biosciences, University of
More informationWhat is the central dogma of biology?
Bellringer What is the central dogma of biology? A. RNA DNA Protein B. DNA Protein Gene C. DNA Gene RNA D. DNA RNA Protein Review of DNA processes Replication (7.1) Transcription(7.2) Translation(7.3)
More informationSupplementary Materials for
advances.sciencemag.org/cgi/content/full/3/4/e1600663/dc1 Supplementary Materials for A dynamic hydrophobic core orchestrates allostery in protein kinases Jonggul Kim, Lalima G. Ahuja, Fa-An Chao, Youlin
More information23-. Shoot and root development depend on ratio of IAA/CK
Balance of Hormones regulate growth and development Environmental factors regulate hormone levels light- e.g. phototropism gravity- e.g. gravitropism temperature Mode of action of each hormone 1. Signal
More informationNOTE: LOOK ON MY WEBSITE FOR THE MUSCLE LABELING POWER POINT/PDF Part I. Identify the parts of the neuron that are labeled below.
Anatomy & Physiology Nervous System Part I 2/26/16 NOTE: LOOK ON MY WEBSITE FOR THE MUSCLE LABELING POWER POINT/PDF Part I. Identify the parts of the neuron that are labeled below. 1. 2. 3. 5. 4. 6. Part
More informationReception The target cell s detection of a signal coming from outside the cell May Occur by: Direct connect Through signal molecules
Why Do Cells Communicate? Regulation Cells need to control cellular processes In multicellular organism, cells signaling pathways coordinate the activities within individual cells that support the function
More informationOrganisms are made up of specialized cells.
All living things are made up of cells! Specialized cells develop from a single zygote Organisms are made up of specialized cells. Each has a specific job/function red blood cell nerve cell Zygotes (fertilized
More informationHuman Coagulation Factor X Total Antigen ELISA Kit
Human Coagulation Factor X Total Antigen ELISA Kit Catalog No: IHFXKT-TOT Lot No: SAMPLE INTENDED USE This human coagulation Factor X antigen assay is intended for the quantitative determination of total
More informationCURRICULUM MAP. TIME CONTENT PAGE REF. SKILLS ASSESSMENT/ACTIVITIES Day 1-3
CURRICULUM MAP COURSE TITLE: Anatomy and Physiology DESCRIPTION TEXTBOOK Essentials of Anatomy and Physiology OF COURSE: 8 th ed: Elaine Marieb Integrated approach to human anatomy. Performance and lab
More informationRaghuram et al PNAS 100:9620
ION CHANNL MACROMOLCULAR COMPLXS A V I R W c g I S L S R d L L LLL I VA S f L LS HP a b D G C S1 S2 S3 S4 S5 I e V N S6 COOH NH 2 PDZ domains; recognition of short peptides with a COOH terminal hydrophobic
More informationBiology Kevin Dees. Chapter 8 Introduction to Metabolism
Chapter 8 Introduction to Metabolism Defined as the sum total of the chemical reactions that occur in a living thing. Think of metabolism as a road map of thousands of different chemical reactions regulate
More informationAffinity labels for studying enzyme active sites. Irreversible Enzyme Inhibition. Inhibition of serine protease with DFP
Irreversible Enzyme Inhibition Irreversible inhibitors form stable covalent bonds with the enzyme (e.g. alkylation or acylation of an active site side chain) There are many naturally-occurring and synthetic
More informationNisa Rachmania Mubarik Major Microbiology Department of Biology, IPB. Fisiologi Molekuler (Nisa RM) 1
Nisa Rachmania Mubarik Major Microbiology Department of Biology, IPB Fisiologi Molekuler (Nisa RM) 1 ENZYMES ARE: Proteins (note that recent developments indicate that both RNA and antibodies may have
More informationLecture 10: Cyclins, cyclin kinases and cell division
Chem*3560 Lecture 10: Cyclins, cyclin kinases and cell division The eukaryotic cell cycle Actively growing mammalian cells divide roughly every 24 hours, and follow a precise sequence of events know as
More informationCellular Electrophysiology and Biophysics
BIOEN 6003 Cellular Electrophysiology and Biophysics Modeling of Force Development in Myocytes II Frank B. Sachse, University of Utah Overview Experimental Studies Sliding Filament Theory Group work Excitation-Contraction
More informationSHORT ANSWER. Write the word or phrase that best completes each statement or answers the question.
ch 2 chemical basis of life Name SHORT ANSWER. Write the word or phrase that best completes each statement or answers the question. Fill in the blank or provide a short answer: 1) When a change in matter
More informationScientists have been measuring organisms metabolic rate per gram as a way of
1 Mechanism of Power Laws in Allometric Scaling in Biology Thursday 3/22/12: Scientists have been measuring organisms metabolic rate per gram as a way of comparing various species metabolic efficiency.
More informationChapter 8 Introduction to Metabolism. Metabolism. The sum total of the chemical reactions that occur in a living thing.
Chapter 8 Introduction to Metabolism Metabolism The sum total of the chemical reactions that occur in a living thing. Think of metabolism as a road map of thousands of different chemical reactions Enzymes
More informationENZYMES. by: Dr. Hadi Mozafari
ENZYMES by: Dr. Hadi Mozafari 1 Specifications Often are Polymers Have a protein structures Enzymes are the biochemical reactions Katalyzers Enzymes are Simple & Complex compounds 2 Enzymatic Reactions
More informationBiochemical bases for energy transformations. Biochemical bases for energy transformations. Nutrition 202 Animal Energetics R. D.
Biochemical bases for energy transformations Biochemical bases for energy transformations Nutrition 202 Animal Energetics R. D. Sainz Lecture 02 Energy originally from radiant sun energy Captured in chemical
More informationCAVITY QUANTUM ELECTRODYNAMICS
CAVITY QUANTUM ELECTRODYNAMICS Edited by Paul R. Berman Department of Physics University of Michigan Ann Arbor, Michigan ACADEMIC PRESS, INC. Harcourt Brace & Company, Publishers Boston San Diego New York
More informationChapter 2 Concepts of Chemistry
Anatomy Physiology and Disease for the Health Professions 3rd Edition Booth Test Bank Full Download: http://testbanklive.com/download/anatomy-physiology-and-disease-for-the-health-professions-3rd-edition-booth-te
More informationFranklin County Page: Calendar For Honorable I. I. LAMKE
Friday 07/0/205. 3AB-PN0030 PATRICIA BOAZ V JOHN T BOAZ Filing : 22-May-203 PETP PATRICIA BOAZ APET SAMANTHA C WACKER RESP JOHN T BOAZ ARES DAVID JEFFERY FERMAN HEARING ON RENEWAL OF FULL ORDER 2. 4AB-PN00649
More informationUNIT 6 THE MUSCULAR SYSTEM
UNIT 6 THE MUSCULAR SYSTEM I. Functions of Muscular System A. Produces Movement Internal vs. External «locomotion & manipulation «circulate blood & maintain blood pressure «move fluids, food, baby B. Maintaining
More informationA MOLECULAR DESCRIPTION OF NERVE TERMINAL FUNCTION
Ann. Rev. Biochem. 1983.52:871-926 Copyright 1983 by Annual Reviews Inc. All r~hts reserved A MOLECULAR DESCRIPTION OF NERVE TERMINAL FUNCTION Louis F. Reichardt and Regis B. Kelly Department of Physiology
More informationScores. Place Name Points
MEN S OPEN 1 Martin Quirke 76 4 20 0 20 0 17 0 0 19 0 2 Keith Brown 75 4 17 18 19 19 0 12 19 0 17 3 Ben Brown 64 4 7 11 14 0 6 19 20 0 8 4 Crawford Lindsay 62 4 0 14 16 0 16 13 0 0 16 5 Andrew Lindsay
More informationENZYME KINETICS. Medical Biochemistry, Lecture 24
ENZYME KINETICS Medical Biochemistry, Lecture 24 Lecture 24, Outline Michaelis-Menten kinetics Interpretations and uses of the Michaelis- Menten equation Enzyme inhibitors: types and kinetics Enzyme Kinetics
More informationFragment-based Approaches in Drug Discovery
Fragment-based Approaches in Drug Discovery Edited by Wolfgang Jahnke and Daniel A. Erianson WILEY- VCH WILEY-VCH Verlag GmbH & Co. KGaA Contents Preface XV A Personal Foreword List of Contributors XVII
More information4 Examples of enzymes
Catalysis 1 4 Examples of enzymes Adding water to a substrate: Serine proteases. Carbonic anhydrase. Restrictions Endonuclease. Transfer of a Phosphoryl group from ATP to a nucleotide. Nucleoside monophosphate
More informationPROTEIN AND PEPTIDE MASS SPECTROMETRY IN DRUG DISCOVERY
PROTEIN AND PEPTIDE MASS SPECTROMETRY IN DRUG DISCOVERY Edited By Michael L. Gross Washington University Guodong Chen Bristol-Myers Squibb Birendra N. Pramanik Merck Research Laboratories JOHN WILEY &
More informationThe diagram below represents levels of organization within a cell of a multicellular organism.
STATION 1 1. Unlike prokaryotic cells, eukaryotic cells have the capacity to a. assemble into multicellular organisms b. establish symbiotic relationships with other organisms c. obtain energy from the
More information4. Which of the following organelles digests waste using hydrolytic enzymes:
Multichoice questions section. You must answer ALL questions. 1. A cell contains many organelles, each of which has a specific function. What is function of mitochondria? a) production of plasma membrane
More informationTeacher Instructions
Teacher Instructions To print handouts for students Go to File print, change Print what: to handouts, change # per page if desired to enlarge slides on page Change Print range to slides and type in slide
More informationSupporting Information Converter domain mutations in myosin alter structural kinetics and motor function. Hershey, PA, MN 55455, USA
Supporting Information Converter domain mutations in myosin alter structural kinetics and motor function Laura K. Gunther 1, John A. Rohde 2, Wanjian Tang 1, Shane D. Walton 1, William C. Unrath 1, Darshan
More informationBASIC BIOLOGICAL PRINCIPLES
BASIC BIOLOGICAL PRINCIPLES A1 A1. Basic Biological Principles 1. Describe the characteristics of life shared by all prokaryotic and eukaryotic organisms 2. Compare cellular structures and their function
More informationChapter 6 Chemistry in Biology
Section 1: Atoms, Elements, and Compounds Section 2: Chemical Reactions Section 3: Water and Solutions Section 4: The Building Blocks of Life Click on a lesson name to select. 6.1 Atoms, Elements, and
More informationCayman Islands Judiciary
Justice Alistair Malcolm 9/25/2017 IND0029/2017 Nolle prosequi Barnes, Joshua Total for 9/25/2017 1 10/2/2017 IND0038/2017 Nolle prosequi Bush, Aaron Total for 10/2/2017 1 10/11/2017 IND0073/2013 Imprisonment
More informationChapter 2 The Chemistry of Life
Chapter 2 The Chemistry of Life I. Water Liquid Naturally occurring It expands liquid to solid Covers more than 75% of our surface Most abundant in living organisms most important inorganic compound for
More informationAn Introduction to Metabolism
An Introduction to Metabolism I. All of an organism=s chemical reactions taken together is called metabolism. A. Metabolic pathways begin with a specific molecule, which is then altered in a series of
More informationUnit 3. Enzymes. Catalysis and enzyme kinetics.
Unit 3 Enzymes. Catalysis and enzyme kinetics. OUTLINE 3.1. Characteristics of biological catalysts. Coenzymes, cofactors, vitamins Enzyme nomenclature and classification 3.2. Enzyme catalysis. Transition
More information2.1 Atoms, Ions, and Molecules
2.1 Atoms, Ions, and Molecules Living things consist of atoms of different elements. An atom is the smallest basic unit of matter. An element is one type of atom. 6 elements make up 99% of all living things
More informationTollemache Cup Kent results
Year Position Group/final Players Tollemache Cup Kent results 1950?/14 South 1951 4/13 South 1952 1953?/7 Southern Harry Ingram Dorothy Pearson and others 1954 1955 1956 4/9 South East 1957 4/8 South East
More informationUnit 2: Chemistry Test Review
Name: Period: Unit 2: Chemistry Test Review 1. List the three states of matter. 2. Describe an atom in terms of its nucleus, valence,shell, electrons, protons, and neutrons. 3. Define the term element
More informationHANDBOOK OF DRUG ANALYSIS
HANDBOOK OF DRUG ANALYSIS APPLICATIONS IN FORENSIC AND CLINICAL LABORATORIES Ray H. Liu Daniel E. Gadzala American Chemical Society Washington, DC Contents Dedication Foreword Preface About the Authors
More informationCells and Their Organelles
Cells and Their Organelles The cell is the basic unit of life. The following is a glossary of animal cell terms. All cells are surrounded by a cell membrane. The cell membrane is semipermeable, allowing
More informationSignal Transduction Phosphorylation Protein kinases. Misfolding diseases. Protein Engineering Lysozyme variants
Signal Transduction Phosphorylation Protein kinases Misfolding diseases Protein Engineering Lysozyme variants Cells and Signals Regulation The cell must be able to respond to stimuli Cellular activities
More informationScaling and Uncertainty Analysis in Ecology
Scaling and Uncertainty Analysis in Ecology Methods and Applications Edited by JIANGUO WU Arizona State University, Tempe, AZ, U.S.A. K. BRUCE JONES US Environmental Protection Agency, Las Vegas, U.S.A.
More informationPolyethylene Glycol (PEG), High Sensitive ELISA
K-ASSAY Polyethylene Glycol (PEG), High Sensitive ELISA For the high sensitive quantitative determination of PEG and PEGylated proteins in serum or plasma Cat. No. KT-657 For Research Use Only. 1 K-ASSAY
More informationName: Date: Hour:
Name: Date: Hour: 1 2 3 4 5 6 Comprehension Questions 1. At what level of organization does life begin? 2. What surrounds all cells? 3. What is meant by semipermeable? 4. What 2 things make up the cell
More informationBMB Lecture 7. Allostery and Cooperativity
BMB 178 2017 Lecture 7 October 18, 2017 Allostery and Cooperativity A means for exquisite control Allostery: the basis of enzymatic control From the Greek: allos = other stereos = solid or space Action
More informationCh 4: Cellular Metabolism, Part 1
Developed by John Gallagher, MS, DVM Ch 4: Cellular Metabolism, Part 1 Energy as it relates to Biology Energy for synthesis and movement Energy transformation Enzymes and how they speed reactions Metabolism
More informationChem Lecture 10 Signal Transduction
Chem 452 - Lecture 10 Signal Transduction 111202 Here we look at the movement of a signal from the outside of a cell to its inside, where it elicits changes within the cell. These changes are usually mediated
More informationInterpreting and evaluating biological NMR in the literature. Worksheet 1
Interpreting and evaluating biological NMR in the literature Worksheet 1 1D NMR spectra Application of RF pulses of specified lengths and frequencies can make certain nuclei detectable We can selectively
More informationEnzyme Kinetics: The study of reaction rates. For each very short segment dt of the reaction: V k 1 [S]
Enzyme Kinetics: The study of reaction rates. For the one-way st -order reaction: S the rate of reaction (V) is: V P [ P] moles / L t sec For each very short segment dt of the reaction: d[ P] d[ S] V dt
More informationf) Adding an enzyme does not change the Gibbs free energy. It only increases the rate of the reaction by lowering the activation energy.
Problem Set 2-Answer Key BILD1 SP16 1) How does an enzyme catalyze a chemical reaction? Define the terms and substrate and active site. An enzyme lowers the energy of activation so the reaction proceeds
More informationBiology: Life on Earth
Biology: Life on Earth Eighth Edition Lecture for Chapter 11 The Continuity of Life: Cellular Reproduction Cellular Reproduction Intracellular activity between one cell division to the next is the cell
More informationBiochemistry 462a - Enzyme Kinetics Reading - Chapter 8 Practice problems - Chapter 8: (not yet assigned); Enzymes extra problems
Biochemistry 462a - Enzyme Kinetics Reading - Chapter 8 Practice problems - Chapter 8: (not yet assigned); Enzymes extra problems Introduction Enzymes are Biological Catalysis A catalyst is a substance
More informationChapter 02 The Chemical Basis of Life
Chapter 02 The Chemical Basis of Life Multiple Choice Questions 1. The amount of matter in an object is its A. element. B. mass. C. ionic charge. D. atomic number. E. weight. HAPS Objective: C01.01d Distinguish
More informationBiochemistry. Biochemistry 9/20/ Bio-Energetics. 4.2) Transport of ions and small molecules across cell membranes
9/20/15 Biochemistry Biochemistry 4. Bio-Energetics 4.2) Transport of ions and small molecules across cell membranes Aquaporin, the water channel, consists of four identical transmembrane polypeptides
More informationCh 3: Chemistry of Life. Chemistry Water Macromolecules Enzymes
Ch 3: Chemistry of Life Chemistry Water Macromolecules Enzymes Chemistry Atom = smallest unit of matter that cannot be broken down by chemical means Element = substances that have similar properties and
More informationthebiotutor.com A2 Biology Unit 5 Responses, Nervous System & Muscles
thebiotutor.com A2 Biology Unit 5 Responses, Nervous System & Muscles 1 Response Mechanism tropism Definition A growth movement of part of plant in response to a directional stimulus examples Positive:
More informationBahnson Biochemistry Cume, April 8, 2006 The Structural Biology of Signal Transduction
Name page 1 of 6 Bahnson Biochemistry Cume, April 8, 2006 The Structural Biology of Signal Transduction Part I. The ion Ca 2+ can function as a 2 nd messenger. Pick a specific signal transduction pathway
More informationLectures by Kathleen Fitzpatrick
Chapter 10 Chemotrophic Energy Metabolism: Aerobic Respiration Lectures by Kathleen Fitzpatrick Simon Fraser University Figure 10-1 Figure 10-6 Conversion of pyruvate The conversion of pyruvate to acetyl
More informationBiochemistry. Biochemistry 7/11/ Bio-Energetics. 4.2) Transport of ions and small molecules across cell membranes
Biochemistry Biochemistry 4. Bio-Energetics 4.2) Transport of ions and small molecules across cell membranes Aquaporin, the water channel, consists of four identical transmembrane polypeptides Key Energy
More information5.111 Principles of Chemical Science
MIT penourseware http://ocw.mit.edu 5.111 Principles of hemical Science Fall 2008 For information about citing these materials or our Terms of Use, visit: http://ocw.mit.edu/terms. 5.111 Lecture Summary
More informationBBS501 Section 1 9:00 am 10:00 am Monday thru Friday LRC 105 A & B
BBS501 Section 1 9:00 am 10:00 am Monday thru Friday LRC 105 A & B Lecturers: Dr. Yie-Hwa Chang Room M130 Phone: #79263 E-mail:changy@slu.edu Dr. Tomasz Heyduk Room M99 Phone: #79238 E-mail: heydukt@slu.edu
More informationGetting In and Out of Mitosis*
Open Access NOBEL LAUREATE PERSPECTIVE Rambam Maimonides Medical Journal Getting In and Out of Mitosis* Tim Hunt, Ph.D., F.R.S. Nobel Laureate in Physiology or Medicine, 2001; Cancer Research UK, London
More information