Lecture 5: Determining Sample Size
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1 Lecture 5: Determining Sample Size Montgomery: Section 3.7 and Lecture 5 Page 1
2 Choice of Sample Size: Fixed Effects Can determine the sample size for OverallF test Contrasts of interest For simplicity, typically assumen i s constant, i.e., n 1 = n 2 = = n a = n Recall Type I error rate: α = P(RejectH 0 H 0 ) Type II error rate: β = P(AcceptH 0 H 1 ) Power = P(RejectH 0 H 1 ) = 1-β Need to know Test Statistics Distr. of test statistics underh 0 = Reject Region (for givenα) Distr. of test statistics underh 1 = power = P(Reject Region H 1 ) 2 Lecture 5 Page 2
3 α = Pr(F 0 > F α,a 1,N a H 0 ) β = Pr(F 0 < F α,a 1,N a H 1 ) Determining Power forf Test Need to know distribution off 0 whenh 1 is true Can showf 0 = MS Trt /MS E F a 1,N a (δ) δ = n τ 2 i /σ2 (non-centrality parameter) Recall E(MS Trt )=σ 2 +n τ 2 i /(a 1) δ = {E(MS Trt ) E(MS E )} df Trt /E(MS E ) Need to specify{τ i } (Note the zero-sum constraint: a i=1 τ i = 0) Power will vary for different choices 3 Lecture 5 Page 3
4 Power Calculation forf Test Givenα,a, andn, can determinef α,a 1,N a Given some value ofδ, can use noncentralf to compute power In SAS, use functionprobf Power=1-PROBF(F α,a 1,N a,a 1,N a,δ) Montgomery: OCC given in Chart V Plotsβ vsφ Φ 2 = δ/a = n τ 2 i /(aσ2 ) Can use charts to determine power or sample size 4 Lecture 5 Page 4
5 1. Choose treatment means (µ+τ i ) Methods to Determineδ orφ 2 Solve for{τ i } and computeφ 2 orδ Difficult to know what means to select 2. Take a mimimum difference approach Suppose there exists a pair of (i,j) such that τ i τ j D The minimum value: Φ 2 = nd 2 /(2aσ 2 ) (e.g., {τ i } = { D/2,0,...,0,D/2}) Power of test is at least1 β 3. Specify a standard deviation increase in percentage (P ) UnderH 1, variance of a randomly choseny i isσ 2 y = σ 2 + τ 2 i /a Randomly chosenτ i has mean 0 and variance τi 2 /a ( P = σ2 + ) τi 2/a/ σ δ = an{(1+.01p) 2 1} Φ 2 = n{(1+.01p) 2 1} 5 Lecture 5 Page 5
6 Power Calculation for Specific Contrast Often with an experiment, a researcher is primarily interested in just a few comparisons or contrasts. In these cases, it can be preferable to determine sample size for these rather than the overallf test. This reduces problem back to thettest situation Need to determine Difference of importance Standard error of comparison May want/need to adjust for multiple comparisons Montgomery describes confidence interval approach Consider pairwise difference in treatment means Specify length of(1 α) 100% confidence interval Length/2 =t α/2,n a 2MS E n Based on the choice of MS E, findn 6 Lecture 5 Page 6
7 Example 3.1 Etch Rate (Page 75) Consider new experiment to investigate 5 RF power settings equally spaced between 180 and 200 W Wants to determine sample size to detect a mean difference ofd=30 (Å/min) with 80% power Will use Example 3.1 estimates to determine new sample size ˆσ 2 = 333.7,D = 30, andα =.05 Using Table V :Φ 2 = 900 n/( ).27 n n Φ df E β 26% 20% 15% power 74% 80% 85% 7 Lecture 5 Page 7
8 Using SAS :δ = aφ 2 data new; a=5; alpha=.05; d=30; var=333.7; do n=5 to 15; df = a*(n-1); nc = n*d*d/(2*var); fcut = finv(1-alpha,a-1,df); beta = probf(fcut,a-1,df,nc); power = 1-beta; output; end; proc print; var n df nc beta power; run; Obs n df nc beta power ***n=10 needed Lecture 5 Page 8
9 Compare all pairs and detect any difference more than 30 (Å/min) with 80% power A pairwise comparison takest 0 = (Ȳi. Ȳk.)/ 2MSE/n Consider Tukey s adjustment: reject when t 0 > q.05 (5,df E )/ 2 H t 1 0 tdfe (δ) withδ = (µ i µ k )/ 2σ 2 /n, UsePROBMC in SAS to get the quantile for multiple comparisons data new1; a=5; alpha=.05; var=333.7; d=30; do n=8 to 12; df = a*(n-1); nc = d/sqrt(var*2/n); /* crit = tinv(1-alpha/2,df); /* LSD approach*/ crit = probmc("range",.,1-alpha,df,a)/sqrt(2); /*Tukey*/ power=1-probt(crit,df,nc)+probt(-crit,df,nc); output; end; proc print; var n df power; run; Obs n df power *** 11 replicates needed here Lecture 5 Page 9
10 Interested in comparing each setting to 200 W, and detect a difference more than 30 (Å/min) with 80% power A pairwise comparison takest 0 = (Ȳi. Ȳc.)/ 2MSE/n Consider using Dunnett s adjustment: reject when t 0 > d.05 (4,df E ) H t 1 0 tdfe (δ) withδ = (µ i µ k )/ 2σ 2 /n, data new1; a=5; alpha=.05; var=333.7; d=30; do n=7 to 12; df = a*(n-1); nc = d/sqrt(var*2/n); crit = probmc("dunnett2",.,1-alpha,df,a-1); /*Two-Sided Dunnett*/ power=1-probt(crit,df,nc)+probt(-crit,df,nc); output; end; proc print; var n df power; run; Obs n df power ***Only 9 replicates needed here Lecture 5 Page 10
11 Use ofproc POWER in SAS PROC POWER andproc GLMPOWER both calculate a i=1 τ2 i using pre-specified treatment meansµ i proc power; onewayanova test=overall power=.80 npergroup=. stddev=18.27 groupmeans = ; run; quit; Overall F Test for One-Way ANOVA Fixed Scenario Elements Method Exact Group Means Standard Deviation Nominal Power 0.8 Alpha 0.05 Computed N Per Group Actual Power N Per Group Lecture 5 Page 11
12 NeitherPROC POWER norproc GLMPOWER can easily do multiple comparison adjustment proc power; onewayanova test=contrast power=.80 npergroup=. stddev=18.27 groupmeans = contrast = ( ); run; quit; Single DF Contrast in One-Way ANOVA Fixed Scenario Elements Method Exact Contrast Coefficients Group Means Standard Deviation Nominal Power 0.8 Number of Sides 2 Null Contrast Value 0 Alpha 0.05 Computed N Per Group Actual Power N Per Group Lecture 5 Page 12
13 Use ofproc GLMPOWER in SAS data trtmeans; input trt resp datalines; ; proc glmpower data=trtmeans; class trt; model resp = trt; contrast trt1 vs. trt5 trt ; power stddev=18.27 alpha=0.05 ntotal=. power=0.8; run; quit; Fixed Scenario Elements Dependent Variable resp Alpha 0.05 Error Standard Deviation Nominal Power 0.8 Computed N Total Index Type Source Test DF Error DF Actual Power N Total 1 Effect trt Contrast trt1 vs. trt Lecture 5 Page 13
14 Choice of Sample Size: Random Effects Can use central F distribution (N a)ms E /σ 2 χ 2 N a (a 1)MS Trt /(σ 2 +nσ 2 τ) χ 2 a 1 ThusF 0 /λ 2 F a 1,N a, whereλ 2 = E(MS Trt) E(MS E ) = 1+nσ 2 τ/σ 2 Power: P(F 0 > F α,a 1,N a σ 2 τ > 0) = P(F > F α,a 1,N a /λ 2 ) Can specify ratio ofσ 2 τ/σ 2 Can specify percentage increasep = ( σ 2 +σ 2 τ/σ 1) 100 OCC given in Chart VI Plotsβ vsλ Use SAS functionprobf power = 1-PROBF(F α,a 1,N a /λ 2,a 1,N a) 14 Lecture 5 Page 14
15 Example: Batch Example on Slide 8 of Lecture 4 Consider new experiment with 5 batches: a random effects problem The variance estimate isσ 2 = 1.8 Desire to detect situation whenστ = 2σ 2 Set power at 80% andα =.05 Using Table VI :λ = 1+2n n λ = df E β 28% 18% 15% power 72% 82% 85% Appears n = 4 gives appropriate power 15 Lecture 5 Page 15
16 Using SAS data new; a = 5; alpha=.05; ratiovar=2.0; do n=2 to 10; df = a*(n-1); lambdasq = 1+ratiovar*n; fcut = finv(1-alpha,a-1,df); beta=probf(fcut/lambdasq,a-1,df); power = 1-beta; output; end; proc print; var n beta power; run; Obs n beta power ** n=4 gives the power Lecture 5 Page 16
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