Outline. Topic 19 - Inference. The Cell Means Model. Estimates. Inference for Means Differences in cell means Contrasts. STAT Fall 2013
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1 Topic 19 - Inference - Fall 2013 Outline Inference for Means Differences in cell means Contrasts Multiplicity Topic 19 2 The Cell Means Model Expressed numerically Y ij = µ i + ε ij where µ i is the theoretical mean of all observations at level i (or in cell i) The ε ij are iid N(0,σ 2 ) which implies the Y ij are independent N(µ i,σ 2 ) Parameters µ 1,µ 2,..., µ r σ 2 Estimates Estimate µ i using the sample mean of the observations at level i ˆµ i = Y i. Pool the sample variances s 2 i using weights proportional to sample size (i.e., df) to get s 2 s 2 = = (ni 1)s 2 i (ni 1) (ni 1)s 2 i n T r Topic 19 3 Topic 19 4
2 Confidence Intervals of µ i s From model Confidence interval Y i. N(µ i,σ 2 /n i ) Y i. ± t(1 α/2;n T r)s/ n i Degrees of freedom larger than n i 1 because pooling variance estimates across treatments (i.e., borrowing information from other groups) SAS Commands data a1; infile u:\.www\datasets525\ch15ta01.txt ; input cases design store; proc means data=a1 mean std stderr clm maxdec=2; var cases; proc glm data=a1; means design/t clm; proc mixed data=a1; lsmeans design / cl; Topic 19 5 Topic 19 6 The GLM Procedure The MEANS Procedure Analysis Variable : cases Lower 95% Upper 95% Des N Mean StdDev StdErr CL for Mean Note: = Except for rounding, this is equal to SSE. Also, 19-4=15 which is the df error in the ANOVA table. t Confidence Intervals for cases Critical Value of t % Confidence design N Mean Limits There is no pooling of error (or df) when computing these confidence intervals. These confidence intervals are often narrower due to the increase in degrees of freedom. Results can vary if there does not appear to be a common variance. Topic 19 7 Topic 19 8
3 The Mixed Procedure Covariance Parameter Estimates Cov Parm Estimate Residual Least Squares Means Standard Design Estimate Error DF t Value Pr > t Lower Upper < < < < Multiplicity Have generated r confidence intervals Overall confidence level (all intervals contain its true mean) is less than 1 α Many different approaches have been proposed Previously discussed using Bonferroni These confidence intervals are the same as the previous page. Standard errors, based on constant variance assumption, are provided. Topic 19 9 Topic Bonferroni t Confidence Intervals for cases proc glm data=a1; means design/bon clm; proc mixed data=a1; lsmeans design /alpha=0.125 cl; SAS Commands Critical Value of t Simultaneous 95% design N Mean Confidence Limits Topic Topic
4 The Mixed Procedure Covariance Parameter Estimates Cov Parm Estimate Residual Hypothesis Tests on µ i s Not usually done SAS typically gives output for H 0 : µ i = 0 which rarely is of any interest Least Squares Means Standard Design Estimate Error DF t Value Pr > t Alpha Lower Upper < < < < If interested in H 0 : µ i = c, it is easiest to subtract of c from all observations in a data step and then test whether the new mean is equal to zero. Can also use CI to make decision Topic Topic From model Differences in means ( 1 Y i. Y k. N (µ i µ k,σ )) ni nk Confidence interval Y i. Y k. ± t(1 α/2;n T r)s 1/n i + 1/n k In this case H 0 : µ i µ k = 0 is of interest Similar multiplicity problem Now have r(r 1) 2 pairwise comparisons to consider Multiplicity Adjustment Approaches adjust multiplier of the SE Alter α level (e.g., Bonferroni) Use different distribution Conservative strong control of overall Type I error - avoids false positives Powerful able to pick up differences that exist - avoids false negatives All approaches try to strike to strike some sort of balance Topic Topic 19 15
5 Least Significant Difference Simply ignores multiplicity issue Most powerful of the procedures but also results in most false positives Uses t(1 α/2;n T r) to determine multiplier Called T or LSD in SAS Tukey Based on studentized range distribution q Range is max(y i ) min(y i ) in r levels Accounts for any possible pair being furthest apart Controls overall experimentwise error rate Uses q(1 α;r,n T r)/ 2 to determine multiplier Called TUKEY in SAS Topic Topic Scheffe Based on the F distribution Accounts for multiplicity for all linear combinations of means, not just pairwise comparisons Protects against data snooping Uses (r 1)F(1 α;r 1,n T r) to determine multiplier Called SCHEFFE in SAS Replaces α by Bonferroni α = α r(r 1)/2 Uses t(1 α /2;n T r) to determine multiplier Called BON in SAS Topic Topic 19 19
6 Holm Refinement of Bonferroni Instead of using α = α g for all comparisons Rank unadjusted P-values from smallest to largest Continue to reject until P k α/(g k + 1) Available in Proc Multtest in SAS False Discovery Rate FDR defined as expected proportion of false positives in the collection of rejected null hypotheses Becoming more popular, especially when # of tests in the thousands or millions Rank P-values from smallest to largest Continue to reject until P k kα/g Available in Proc Multtest in SAS Topic Topic SAS Commands proc glm data=a1; means design/lsd tukey bon scheffe; means design/lines tukey; proc mixed data=a1; lsmeans design / diff=all; lsmeans design / adjust=tukey; lsmeans design / adjust=bon; lsmeans design / adjust=scheffe; proc glimmix data=a1; lsmeans design / adjust=tukey lines; t Tests (LSD) for cases NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Critical Value of t Comparisons significant at the 0.05 level are indicated by ***. Difference design Between 95% Confidence Comparison Means Limits *** *** *** *** *** *** *** *** *** *** Topic Topic 19 23
7 Tukey s Studentized Range (HSD) Test for cases NOTE: This test controls the Type I experimentwise error rate. Critical Value of Studentized Range Comparisons significant at the 0.05 level are indicated by ***. Difference design Between Simultaneous 95% Comparison Means Confidence Limits *** *** *** *** *** *** Topic Bonferroni (Dunn) t Tests for cases NOTE: This test controls the Type I experimentwise error rate, but it generally has a higher Type II error rate than Tukey s for all pairwise comparisons. Critical Value of t Comparisons significant at the 0.05 level are indicated by ***. Difference design Between Simultaneous 95% Comparison Means Confidence Limits *** *** *** *** *** *** Topic Scheffe s Test for cases NOTE: This test controls the Type I experimentwise error rate, but it generally has a higher Type II error rate than Tukey s for all pairwise comparisons. Critical Value of F Comparisons significant at the 0.05 level are indicated by ***. Difference design Between Simultaneous 95% Comparison Means Confidence Limits *** *** *** *** *** *** Topic Tukey s Studentized Range (HSD) Test for cases Critical Value of Studentized Range Minimum Significant Difference Harmonic Mean of Cell Sizes NOTE: Cell sizes are not equal. Means with the same letter are not significantly different. Mean N design A B B B B B Topic 19 27
8 Mixed Standard Effect design _design Estimate Error DF t Value Pr > t Adjustment Adj P design design design < design design < design design Tukey-Kramer design Tukey-Kramer design <.0001 Tukey-Kramer design Tukey-Kramer design <.0001 Tukey-Kramer <.0001 design Tukey-Kramer design Bonferroni design Bonferroni design <.0001 Bonferroni design Bonferroni design <.0001 Bonferroni <.0001 design Bonferroni design Scheffe design Scheffe design <.0001 Scheffe design Scheffe design <.0001 Scheffe <.0001 design Scheffe Glimmix Differences of design Least Squares Means Adjustment for Multiple Comparisons: Tukey-Kramer Standard design _design Estimate Error DF t Value Pr > t Adj P < <.0001 < Tukey-Kramer Grouping for design Least Squares Means (Alpha=0.05) LS-means with the same letter are not significantly different. design Estimate A B B B B B Topic Topic SAS Commands proc multtest data=a1 holm fdr out=new noprint; contrast ; contrast ; contrast ; contrast ; contrast ; contrast ; test mean(cases); proc print data=new; Obs _test var contrast value se nval_ raw_p stpbon_p fdr_p 1 MEAN cases MEAN cases MEAN cases MEAN cases MEAN cases MEAN cases Instead of comparing each raw P-value to a different α level, the P-values are adjusted based on the procedure. This approach works for experiments with a small number of levels. Can also input a set of P-values and perform the analysis. Topic Topic 19 31
9 Linear Combination of Means Would like to test H 0 : L = c i µ i = L 0 Hypotheses usually planned but can be after the fact Can use statistical model to construct t-test L = c i Y i. Var( L) = Var( c i Y i. ) t = L L 0 Var( L) = c 2 ivar(y i. ) = MSE (c 2 i /n i ) Contrasts Special case of linear combination Requires c i = 0 Example 1: µ 1 µ 2 = 0 Example 2: µ 1 (µ 2 + µ 3 )/2 = 0 Example 3: (µ 1 + µ 2 ) (µ 3 + µ 4 ) = 0 Under H 0 : t t nt r Topic Topic SAS Commands proc glm data=a1; contrast 1&2 v 3&4 design ; estimate 1&2 v 3&4 design ; *Joint test of several contrasts; proc glm data=a1; contrast 1 v 2&3&4 design ; estimate 1 v 2&3&4 design /divisor=3; contrast 2 v 3 v 4 design , design ; Contrast DF Contrast SS Mean Square F Value Pr > F 1&2 v 3& <.0001 Standard Parameter Estimate Error t Value Pr > t 1&2 v 3& <.0001 Contrast does an F test while Estimate does a t-test and gives an estimate of the linear combination. Contrast allows you to simultaneously test a collection of contrasts. Contrast DF Contrast SS Mean Square F Value Pr > F 1 v 2&3& v 3 v <.0001 Topic Topic 19 35
10 Background Reading KNNL Sections knnl738.sas KNNL Chapter 18 Topic 19 36
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