UFN Úvod do funkcionalizace nanomateriálů

Transcription

1 UFN Úvod do funkcionalizace nanomateriálů

2 Zápočet, zkouška 2 Cvičení RNDr. Michal Řezanka, Ph.D. exkurze na konci semestru zápočet za 100% účast (v případě neúčasti referát na probírané téma) Přednášky RNDr. Michal Řezanka, Ph.D. ústní zkouška ve zkouškovém období

3 Náplň, doporučená literatura 3 Sapsford, K.-E. et al. Chem. Rev. 2013, 113,

4 one dimension < 100 nm (1 m) Nanomaterials 4 Amin, M. T. et al. Adv. Mater. Sci. Eng. 2014,

5 Nanomaterials 5

6 NPs preparation 6 Zahmakıran, M. et al. Nanoscale, 2011, 3,

7 Supercritical atomization 7 2 m Jovanovic, N. et al. Pharm. Res., 2004, 11,

8 Nanoparticles (NPs) 8 Sajanlal P. R. et al. Nano Rev. 2011, 2, 5883.

9 Au and Ag NPs 9 Au nanoparticles 100 nm Au nanoshapes Au nanocages Au nanoframes Ag nanocarrot Ag nanorice Liang, H.-Y. et al. Front. Phys. 2016, 11, Sajanlal P. R. et al. Nano Rev. 2011, 2, 5883.

10 Au NPs 10 2D and 3D TEM characterization of hybrid nanostructures containing different gold nanoparticles coated with mesoporous silica, after growing gold tips through the silica channels: nanospheres (a,e), singlecrystal nanorods (b,f), pentatwinned nanorods (c,g) and nanotriangles (d,h). All the images on the left panel were obtained at the same magnification. At the right panel, a visualization of the 3D reconstructions for a representative nanoparticle of each type is displayed, where the radial nature of both the channels and the gold branches can be observed. Sanz-Ortiz, M. N. et al. ACS Nano, 2015, 9,

11 Surface of NPs 11 = m 2

12 Surface atoms 12 < 4 nm > 10 nm Klabunde, K. J. et al. J. Phys. Chem. 1996, 100, Rao, C. N. R. et al. Chem. Eur. J. 2002, 8,

13 Analýza nanočástic 13 SEM TEM AFM DLS Billingsley, D. J. et al. Phys. Biol. 2012, 9,

14 Nanomaterials in nature 14 Tobacco mosaic virus capsida Butterfly wing Gecko feet setae Wax tubes on the lotus leaf

15 History of man-made nanomaterials 15 Lycurgus cup (a) in reflected and (b) transmitted light 4 th cent. AD Alexandria or Italy Au and Ag NPs nm Damascus steel sword 6 th cent. BC India Cementite (Fe 3 C) nanofibers Carbon nanotubes Freestone, I. et al. Gold Bull. 2007, 40,

16 History of nanomaterials 16 4 th 5 th cent. BC: Soluble gold (Egypt and China). Colouring of glass and ceramics Middle Ages medicinal uses against heart and venereal problems, dysentery, epilepsy, and tumours, and for diagnosis of syphilis first functionalisation of Au NPs by starch by H. H. Helcher 1857 first documented preparation of Au NPs by M. Faraday 1959 R. Feynman There s Plenty of Room at the Bottom 1974 N. Taniguchi first use of term nano-technology late 1970s E. Drexler - developed ideas about molecular nanotechnology

17 Nobel prizes in nanomaterials 17 Year Field Laureate Discovery 1925 chemistry R. A. Zsigmondy Colloid chemistry 1932 chemistry I. Langmuir Monomolecular layers 1986 physics G. Binnig a H. Rohrer Scanning microscopy 1996 chemistry R. F. Curl Jr., H. W. Kroto a R. E. Smalley Fullerene 2016 chemistry J.-P. Sauvage, J. F. Stoddart a B. L. Feringa Molecular machines

18 Potential NP bioconjugate components 18 Sapsford, K.-E. et al. Chem. Rev. 2013, 113,

19 Potential NP bioconjugate components 19 Sapsford, K.-E. et al. Chem. Rev. 2013, 113,

20 Principle Bio/Physicochemical Influences on the Interface between Nanomaterials and Biological Systems 20 Sapsford, K.-E. et al. Chem. Rev. 2013, 113,

21 Bioconjugations 21 Sapsford, K.-E. et al. Chem. Rev. 2013, 113,

22 Bioconjugations 22 Sapsford, K.-E. et al. Chem. Rev. 2013, 113,

23 Chemical reactions concept 23 Click chemistry outlined by Kolb, Finn, and Sharpless in 2001 Modular Wide in scope High yield Producing nontoxic or inoffensive byproducts Simple reaction conditions attainable in any chemistry laboratory Readily available starting materials or reagents No solvent or a benign solvent Should allow simple product isolation Bioorthogonal chemistry Subclass of click chemistries Can be applied in a biological environment (where diverse biomolecular functional groups are present) without cross-reactivity. The reaction proceeds in water at or near neutral ph, between 25 and 37 C Do not involve cytotoxic reagents or byproducts Must resist nucleophilic attack, reducing environments, and enzymatic degradation Sapsford, K.-E. et al. Chem. Rev. 2013, 113,