Click to edit Master title style Effect of seed and foliar treatments on vigor of soybean plants Jerseyville, IL

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1 Click to edit Master title style Effect of seed and treatments on vigor of soybean plants Jerseyville, IL Click to edit Master text styles Plant vigor Second level UTC V Third level R2 Fourth level» Fifth level Treated seed UTS + One TS +One UTS + Two TS +Two UTS + Three TS + Three with spary at AC140DC (TS) and and, V3-V4, V3-V4 V3-V4 V3-V4 and R1-R2 and R1-R Plant vigor V3 UTC 6.83 R Treated Seed Average of 4 UTS and 4 TS treatments 1. Early plant vigor was significantly better in the plots with all the 7 seed and treatments compared to UTC. 2. Late plant vigor was also significantly better in the plots with 4 of the 7 seed and treatments (UTS+V2, UTS+V2,V4, UTS+V2,V4,R2, TS+V2,V4,R2) compared to UTC. Growth was also better in the 3 other treatments compared to UTC but the difference was not statistically significant. 12/1/

2 Click to edit Master title style Click to edit Master text styles Second level Third level Plant vigor - Jerseyville, IL trial Fourth level» Fifth level T1 Untreated check T4 = T1 + 12/1/ T5 A140DC Treated seeds T8 = T5 +

3 Click to edit Master title style Plant vigor 24 days after last spray Jerseyville, August 11, 2016 Click to edit Master text styles Second level Third level Fourth level» Fifth level T1 Untreated check T8 = T5 + 12/1/ Plant vigor was better in plots with A140DC treated seeds and three spray (T8) compared to A140DC treated seeds (T5)and untreated check (T1)

4 Click to edit Master title style Effect of seed and treatments on vigor of sorghum plants Jerseyville, IL Click to edit Master text styles Plant vigor Second level Third level UTC V3 Fourth level Treated UTS + seed with One AC140DC (TS) R2» Fifth level TS +One UTS + Two and V3- V4 TS +Two spary at and V3- V4 UTS + Three, V3-V4 and R1-R2 TS + Three, V3-V4 and R1-R2 1. Early plant vigor was significantly better in the plots with 3 of the 7 seed and treatments (UTS+V2, UTS+V2,V4, TS+V2) compared to UTC. Growth was also better in the other 4 treatments compared to UTC but the difference was not statistically significant. 2. Late plant vigor was significantly better in the plots with 5 of the 7 seeds and treatments (UTS+V2, UTS+V2,V4, TS+V2, TS+V2,V4, TS+V2,V4,R2) compared to UTC. Growth was also better in the other 4 treatments compared to UTC but the difference was not statistically significant. 12/1/ Plant vigor UTC V3 R2 Treated Seed Average of 4UTS and 4 TS treatments

5 Click to edit Master title style Click to edit Master text styles Second level Third level Plant vigor Jerseyville, IL trial Fourth level» Fifth level T1 Untreated check T4 = T1 + 12/1/2016 T5 A140DC Treated seeds T8 = T5 + spray 21 at

6 Click to edit Master title style Plant vigor 24 days after last spray Jerseyville, MO Aug 11, 2016 Click to edit Master text styles Second level Third level Fourth level T7» Fifth level T8 T5 T8 T8 T5 T5 Clear difference in plots with treatment 8 and 5. T5 is only seed treatment, and T8 12/1/2016 is seed treatment and three sprays. 22

7 Click to edit Master title style Effect of seed and treatments on grain yield of soybean Jerseyville, IL Click to edit 70 Master text styles 65 Second level Yield (Bushels/ acre) 60 Third level 55 Fourth 52.8a level 60.7bc 50» Fifth level 58.2ab 62.7bc 59.1ab 62.0bc 60.6bc 67.1c 45 UTC Treated seed with AC140DC (TS) UTS + One TS +One UTS + Two and V3-V4 TS +Two spary at and V3-V4 UTS + Three TS + Three, V3-V4, V3-V4 and R1-R2 and R1-R2 1. A140DC seed treatment showed statistically significant differences with UTC, with relative yield increase of 7.9 bushels (15%) over UTC. 2. Combination of seed and treatments presented higher yield response compared to their individual treatments alone. 3. Combination involving seed treatment and 3 applications (at V2, V4 and R2) gave the best response with yield increase of 14.3 bushels (27%) over UTC. This treatment also increased yield by 6.4 bushels (12%) over the seed treatment alone. 12/1/

8 Click to edit Master title style Effect of seed and treatments on sorghum grain yield Jerseyville Click to edit Master text styles 125 Second level yield (Bushels/ Acre) Third 115 level 110 Fourth level a 108.0ab» Fifth level 120.0c 123.4c 117.8bc 119.3bc 119.0bc 125.4c 90 UTC Treated seed with AC140DC (TS) UTS + One TS +One V1- V2 UTS + Two TS +Two spary at V1- and V3- V2 and V3-V4 V4 UTS + Three, V3-V4 and R1-R2 TS + Three, V3-V4 and R1-R2 1. Seed treatment with A140DC increased the yield by 7.3 bushels/acre (7%) over UTC but this increase was not statistically significant. 2. Foliar treatments of A140DC also showed significant difference with UTC with relative yield increase of 17.1 to 19.73bushels/acre (17% to 19%) over UTC. 3. Combination of seed and treatments presented higher yield response compared to these treatments alone. 4. Combination involving seed treatment and 3 applications (at V2, V4 and R2) gave the best response with yield increase of 24.7 bushels/acre (25%) over UTC and 17.4 bushels (17%) over seed treatment alone. 12/1/

9 Click to edit Master title style Effect of seed and treatments on soybean grain quality Jerseyville, IL Click to edit Master text styles Second level Treatments Protein % Third level Total fat % 12/1/ kernel wt (g) UTC UTS + One UTS + Two spray sat and V3-V UTS + Three sprays at, V3-V4 and R1-R Fourth level Treated seed with AC140DC (TS) TS +One » Fifth level TS +Two spray sat and V3-V TS + Three sprays at, V3-V4 and R1-R LSD at P= F value 4.4** NS NS P value CV % No significant effect of any treatment on the total fat and 1000 kernel weight compared to UTC. 2. Numerically total fat was more in the seeds from the plots with treated seeds and three applications compared to UTC. 3. Protein and Kernel weight was more in UTC compared to most of the treatments.

10 42.5 Click to edit Master title style Effect of seed and treatments on soybean grain quality Jerseyville, IL Click to edit Master text styles 21.2 Protein % Second level UTC Third level Treated seed with AC140DC (TS) TS +One UTS + TS +Two Fourth level Two UTS + One and V3-,» Fifth level and V3- V spary at V4 UTS + Three V3-V4 and R1- R2 TS + Three, V3-V4 and R1- R2 Total fat % UTC Treated seed with AC140DC (TS) UTS + One TS +One UTS + Two and V3- V4 TS +Two spary at and V3- V4 UTS + Three, V3-V4 and R1- R2 TS + Three, V3-V4 and R1- R2 Kernel wt (g) UTC Treated seed with AC140DC (TS) UTS + One TS +One UTS + Two and V3- TS +Two spary at and V3-V4 UTS + Three, V3-V4 V4 and R1-R2 12/1/ TS + Three, V3-V4 and R1-R2

11 Click to edit Master title style Effect of seed and treatments on sorghum grain quality Jerseyville, IL Click to edit Master text styles Second level Treatments Protein % Third level Total starch % 12/1/ kernel wt (g) UTC UTS + One UTS + Two sprays at and V3-V UTS + Three sprays at, V3-V4 and R1-R Fourth level Treated seed with AC140DC (TS) TS +One » Fifth level TS +Two sprays at and V3-V TS + Three sprays at, V3-V4 and R1-R LSD at P= F value NS 3.0* NS P value CV % Numerically protein was more in the seeds from the plots with treated seeds compared to UTC but this difference was not statistically significant. 2. Total starch was numerically more in the seeds from the plots with treated seeds alone or in combinations with applications compared to UTC. 3. No significant effect of any treatment on protein and 1000 kernel weight compared to UTC

12 Protein % Click to edit Master title style Effect of seed and treatments on sorghum grain quality Jerseyville, IL Click to edit Master text styles Second level UTC Treated seed with AC140DC (TS) Third level UTS + One Fourth level TS +One UTS + Two spary at» Fifth and and level V3-V4 Kernel wt (g) TS +Two V3-V UTS + Three, V3-V4 and R1-R2 UTC TS + Three, V3-V4 and R1-R2 Treated seed with AC140DC (TS) UTS + One spray at TS +One spray at /1/ Total starch % UTS + Two spray at and V3-V4 UTC TS +Two spary at and V3-V4 Treated seed with AC140DC (TS) UTS + Three, V3- V4 and R1- R2 UTS + One TS + Three spray at, V3-V4 and R1-R2 TS +One UTS + Two and V3- V4 TS +Two spary at and V3- V4 UTS + Three, V3-V4 and R1- R2 TS + Three, V3-V4 and R1- R2

13 NN-Magentar WHEAT AND BARLEY RESULTS South America 2016

14 ) Objective. - Measure under field conditions, yield and biomass changes for the use of MAGNETAR in wheat and barley crops. 2) Trial design and analysis. - Paired plots with the following treatments: 1) Commercial control. 2) MAGNETAR 3 applications (1 seed + 2 during crop development) (time of application Z 12 and Z 30) already applied 3) MAGNETAR 4 applications (1 seed + 3 during crop development). (time of application Z 12, Z 30 and Z 60) 4) MAGNETAR 4 applications (1 seed + 3 during crop development) and 15% less agrichemicals and fertilizers. Until now, only 15% less fertilizer was applied. - Design in field (7 plots, control is used to correct environmental gradient) Control MAGNETAR 3 applications Control MAGNETAR 4 applications Control MAGNETAR 4 applications <15% Control Note: Z 12 = Three leaves; Z 30 = beginning of stem elongation; Z 60 = beginning of earing. - In each plot, we measure 5 times each variable. - For the statistical analysis, we used a design of paired control to correct environmental gradient of site. We used a mixed model and Gaussian correlation to adjust result to environmental gradient using controls. Then we used LSD test to separate means of each variable. - The analysis was done withy R software. 3) Measurements. - Plant population. - Biomass accumulation at the beginning of elongation (Z 30), flowering (Z 60) and harvest. - Differences in pests and weeds during the crop cycle (data no show as no differences were detected).

15 4) Results. 4.1) Plant population. - At the emergency of the crop the only treatment applied was seed treatment. - No differences were detected in barley between treatment (P< 0,3990), but a small difference where detected for wheat (P< 0,0001). - In wheat trial MAGNETAR application have around 10% more plant than control treatment Barley 196 a 193 a Plant Population (pl m -2 ) Plant population (pl m -2 ) Control 200 b Wheat MAGNETAR at seed 219 a 0 Control MAGNETAR at seed Figure 1. Plant population per treatment. Means with common letter are not significantly different (P> 0.05).

16 Biomass acumulation - Z 30 (kg ha -1 ) Biomass acumulation - Z30 (kg ha -1 ) 4.2) Biomass accumulation at the beginning of elongation (Z 30). - No statistically differences were detected between treatment for barley (P< 0,1909) and wheat (P< 0,1413). - Although treatments with MAGNETAR tend to have about 31% and 22% more biomass than control for wheat and barley respectively. - In turn crops with MAGNETAR have a better visual aspect, especially at the beginning of elongation (Image 1) Barley a 793 a 807 a Control MAGNETAR MAGNETAR <15% Fertilizer Wheat a 2085 a 2031 a Control MAGNETAR MAGNETAR <15% Fertilizer Figure 2. Biomass accumulation at the beginning of elongation. Means with common letter are not significantly different (P> 0.05).

17 BARLEY Magnetar Control WHEAT Control Magnetar Control Image 1. Trials at beginning of elongation. 4.3) Biomass accumulation at the beginning of flowering (Z 60). - No statistically differences were detected between treatment for barley (P<0,6879) and wheat (P<0,3968). - For barley, treatments with MAGNETAR tend to have about 6% more biomass than control. - For wheat, as biomass accumulation was higher at this stage, and therefore nutrients demand, treatment with less fertilizer, tend to accumulate less biomass, independently of MAGNETAR. - For this crop, if we compare treatments with full fertilization, MAGNETAR treatment tend to accumulate 8% more biomass than control.

18 Biomass acumulation - Z60 (kg ha-1) Biomass acumulation - Z 60 (kg ha -1 ) 6000 Barley a 5064 a 5089 a Control MAGNETAR MAGNETAR <15% Fertilizer Wheat a 8176 a 7199 a Control MAGNETAR MAGNETAR <15% Fertilizer Figure 3. Biomass accumulation at the beginning of flowering. Means with common letter are not significantly different (P> 0.05). 4.4) Grain yields. - For barley, where detected statistical differences between treatment (P<0.001). - Barley s yield where low consider that is a trial, and this is a consequence of the bad weather conditions at the beginning of trial. - The MAGNETAR treatment with 3 application obtain the maximum yield.

19 Grain yield (kg ha -1 ) - The treatment with MAGNETAR application and less fertilizer obtain the lowest yield. - The fourth application of Magnetar don t rise yields, maybe as consequence of late application (Z 60), but counterparty, have less yields as 3 application treatment without a clear explanation of results Barley c 3720 a 3138 b 2316 d Figure 4. Barley grain yield. Means with common letter are not significantly different (P> 0.05). - For wheat yield, statistical differences were detected (P< 0,0228). - As well as barley, the treatment with 3 applications have the maximum yield and the application of less fertilizer with MAGNETAR have the minimum yield. - In the relation to control, MAGNETAR (3 applications) was 8% higher yields (426 kg ha -1 ).

20 Grain yield (kg ha -1 ) 7000 Wheat b 5798 a 5588 ab 5447 ab Control Magnetar (3 application) Magnetar (4 application) MAGNETAR (4 application) - < 15% Figure 5. Wheat grain yield. Means with common letter are not significantly different (P> 0.05). 5) Total biomass and numeric yield components. - For barley crops, differences in yield were consequence of changes in grain number, the number of spikes and total biomass accumulate at harvest. - For wheat, statistical yield differences, were no explain directly by a variable, as no differences in numerical yield components. Although, the greatest treatment yield, tend to have more total biomass, more spikes and number of grains.

21 Table 1. Total biomass and numeric yield components for barley and wheat crops. Barley Treatmeant Total Biomass (kg ha -1 ) Spikes m -2 Grain number m -2 Grain weight (gr) Control 9751 ab 409 a 6938 b 47,0 MAGNETAR ( a 423 a 7983 a 48,2 MAGNETAR ( ab 402 ab 6702 c 46,9 MAGNETAR (4 app.) - < 15% 8507 b 347 b 5106 c 46,0 P-value 0,0997 0,0033 <0,0001 0,2345 Wheat Control ,7 MAGNETAR ( ,0 MAGNETAR ( ,0 MAGNETAR (4 application) ,0 P-value 0,1614 0,1837 0,4438 0,9096 6) Final comments. - Application of MAGNETAR achieved the greater yield in barley and wheat crops (24% and 8% of increase). - The better treatment was the application of MAGNETAR in three times (seed + Z12 + Z30). - The treatment with application at the beginning of flowering, keep a lower yield without a clear explanation. - Differences were magnified in the more stressed crop (barley) in field. - Differences in yield as consequence of MAGNETAR treatment, were construct with small but a consistent more growth, that determine better condition for crops at grain formation stage. - These results must be re-tested in different years and conditions, but show a MAGNETAR as a possible technology to incorporate in local crops systems, especially in stressful environmental.

22 Universal Bio Program Field test summary Mineral fertilizer Magnetar and soy beans-south America Tests made on three different test sites. Season was very poor with heavy rains reducing normal yields with up to two thirds. Seed treatment with Magnetar, 15gr / 100Kg seed Foliar treatments with Magnetar 150gr / hectare Site A Control Sample Site A Magnetar 3 (V1-2, V5, R1) Yield kg / ha Treatment 2,067 No Magnetar, regular fertiliser program 2,391 Magnetar Foliar Magnetar treatment at stages V1-2, V5, R1 +reg fertilizer program Site B Control Sample Site B Magnetar Seed, 2 (V1-2, V5) 1,642 No Magnetar, regular fertiliser programs 2,242 Magnetar Seed treatment and treatment at stages V1-2, V5 + regular fertiliser programs. Site C Control Sample Site C Magnetar Seed, 3 (V1-2, V5, R1) 1,135 No Magnetar, regular fertiliser programs. 1,819 Magnetar Seed treatment and treatment at stages V1-2, V5, R1 + regular fertiliser programs. info@northernnutrients.com P:

23 Results: With three treatments and no seed treatment: +324kg (+15.6%) With seed treatment + two treatments +600kg (+36.5%) With seed treatment + three treatments : +684kg (+60.2%) A-R egular Co ntrol A-Magnetar 3 no seed B- R egular Co ntrol B-Magnetar Seed + 2 C Regular control C Magnetar Seed + 3 Foliar info@northernnutrients.com P:

24 Northern Nutrients Universal Bio Program Field test summary Mineral fertilizer NN-Magnetar and soy beans South America 2015 Seed treatment with NN-Magnetar, 15gr / 100Kg seed Foliar treatments with NN-Magnetar 150gr / hectare Mass 1000 Yield kg / ha Treatment Control Sample ,083 Regular fertiliser program Magnetar Seed ,249 Magnetar Seed treatment + regular fertiliser Magnetar Seed, ,362 program MAGNETAR Seed treatment, MAGNETAR treatment at R1 +reg fertilizer (R1) Magnetar Seed, 2 (R1, V5) ,669 stage MAGNETAR R1 + regular Seet treatment, fertiliser program MAGNETAR treatment at R1 + V5 + reg fertilizer stages R1, V5 + regular fertiliser program. Results: With only one seed treatment the yield increase was:+166kg (+5.38%) With seed treatment + one treatment the yield increase was: +279kg (+9.04%) With seed treatment + two treatments the yield increase wa +586kg (+19.11%) R egular Co ntrol Magnetar S eed Magnetar S eed + R1 Magnetar Seed+ R1+ V

25 Plot test summary Mineral fertilizer NN-Magnetar and sugar beet by Sugar beet Research Center 2016 Three-year ( ) test. Strip test 2x48m Test 1-3 were all using same fertilizer and herbicide program. Yield kg / ha Treatment 1, Control 34,309 No Magnetar 2, In-Furrow treatment 42,007 1% Magnetar solution at sowing 3, Foliar treatment 39,046 3 treatment 0.075% Magnetar solution 4, Foliar treatment, reduced herbicide 31,908 3 treatment 0.075% Magnetar solution with 25% reduction of herbicide agents *. Results: 2 In-Furrow treatment +7,698kg (+22.4%) 3 Foliar treatment +4,737kg (+12.1%) 4 Foliar treatment -25% herbicide: Poor performing part of field and no proper control. But encouraging results by the accounts of the testing party and tests will continue during the coming two years with separate control. * For 2016 test, no control with reduced herbicide usage

26 Field test summary Magnetar and winter rapeseed-eastern Europe Trial had two test sites. One larger 2x2ha and one smaller 2x1ha with two test plots. The smaller test site suffered from heavy winter damage on plot 1 not experienced in the control field. The larger test site had similar plantstand to its control field. Yields were harvester measured. Tests were done without seed treatment and four treatments. Foliar treatments 1 Magnetar 100gr/ha + Regular program (Delfan Plus, Tradebor, Caryx Biscaya) 2 Magnetar 120gr/ha + Regular program (Ruuter AA) 3 Magnetar0 120gr/ha + Regular program (Tradebor, Delfan Plus, Magnesium sulfate, Biscaya, Tilmor, Spray Plus) 4 Magnetar 100gr/ha + Regular program (Magnesium sulfate, Cantus Gold) Yield kg / ha Oil % Water % Treatment Seed Oil F1 Control 4, No Magnetar, regular fertiliser/protection program F1 Magnetar 5, Control + Magnetar treatment at 1, Autumn rosette phase 2, Spring beginning of vegetation 3, 28 days after 2 nd spray 4, 10 days after 3 rd spray F2 Control 4,800 2, No Magnetar, regular fertiliser/protection program F2-1 Magnetar 4,700 N/A N/A N/A Control + Magnetar treatment at stages 1, Autumn rosette phase 2, Spring beginning of vegetation 3, 28 days after 2 nd spray 4, 10 days after 3 rd spray F2-2 Magnetar 4,900 2, Control + Magnetar treatment at stages 1, Autumn rosette phase 2, Spring beginning of vegetation 3, 28 days after 2 nd spray 4, 10 days after 3 rd spray * Waiting for release confirmation

27 Plot test summary winter Rapeseed, 2017 Results: With four Magnetar treatments and no seed treatment: +600/270kg (+12.8%/+14.2%) With four Magnetar treatments with severe winter damage test fields /51kg (+2.1%/2.5%) F1 Control F2 Control F2 Magnetar F1 Magnetar F1 Control F2 Control F2 Magnetar F1 Magnetar

28 Plot test summary Mineral fertiliser Magnetar / Magnetar-B and winter rapeseed with The Rural Economy and Agricultural Societies, Grästorp, Sweden. Tests made on one test site with two rows and six repetitions. Season was in general fair. Trials was only made with two later stage treatments Foliar treatments with Magnetar 150gr / hectare per treatment. Magnetar multipurpose complex. Foliar treatments with Magnetar-B 300gr / hectare per treatment. Magnetar-B multipurpose complex with additional chelated Boron. Yield kg / ha Stem Oil % Water % Treatment strength Seed Oil (0-100) Row 0 4,540 2, No Magnetar, regular fertiliser/protection program Control Row 1Mag- 4,934 2, Control + Magnetar treatment at stages netar DC51-53 and DC Row 2 Mag- 4,783 2, Control + Magnetar treatment DC51-53 netar + Magnetar-B and Magnetar-B treatment in DC57-59

29 Plot test summary winter Rapeseed, Swedish Rural Economy and Agricultural Societies, Grästorp, Sweden 2017 Results: With two Magnetar treatments and no seed treatment: +394/177kg (+8.7%/+8.2%) With one Magnetar and one Magnetar-B treatments and no seed treatment +243/136kg (+5.4%/6.3%) Control Magnetar & Magnetar-B Magnetar Control Magnetar & Magnetar-B Magnetar