Female-targeted semiochemical baits for the western corn rootworm Diabrotica v. virgifera (Coleoptera: Chrysomelidae)

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1 Femle-trgeted semiochemicl its for the western corn rootworm Dirotic v. virgifer (Coleopter: Chrysomelide) TÓTH, MIKLÓS 1 VUTS, JÓZSEF 1 SZARUKÁN, ISTVÁN 2 JUHÁSZ, ISTVÁN 2 MANAJLOVICS, FERENC 2 1 Plnt Protection Institute, HAS, Budpest, Hungry 2 Derecen University, Ctr. Agric. Res., Derecen, Hungry

2 In recent yers emphsis of semiochemicl KLPflor+ reserch on the western corn rootworm (WCR) ( Dirotic v. virgifer KLP felülrœl LeConte, Coleopter, Chrysomelide) shifted towrds the development of more femle-specific lures. KLPfero+ KLPflor+

3 Through identifying voltile compounds KLPflor+ from mize silk, Hmmck et l (J. Chem. Ecol. 27: , 21) KLP felülrœl climed tht some comintions of their newly identified compounds were more powerful in ttrcting femles thn the conventionl florl its (sed on 4-methoxy cinnmldehyde. KLPfero+ KLPflor+

4 Recently we developed the KLP ( ht ) KLPflor+ trp (introduced t the Brtislv Dirotic conference in 25), which is KLP felülrœl non-sticky trp design nd cn e ited with pheromonl or florl it. KLPfero+ KLPflor+

5 As presented on the Brtislv KLPflor+ vs. PALs: conference, we found tht the KLP trp when femle ited with in the ctch florl it ws especilly suitle for the cpture of femle WCR. men femle rtioo (+SE) normlized ginst rtio in PALs Derecen, 24 KLPflor+ PALs men femle (+SE) July August Septemer Octoer eetles KLPflor+ P< eetles PALs The KLPflor+ cught femles in significntly higher rtio thn the PALs!

6 men femle rtioo (+SE) normlized ginst rtio in PALs KLPflor+ vs. PALs: femle in ctch Derecen, 24 KLPflor+ PALs men femle (+SE) July August Septemer Octoer eetles KLPflor+ P< eetles PALs The KLPflor+ cught femles in significntly higher rtio thn the PALs!

7 Encourged y this we set out to compre efficiency of femletrgeted Dirotic its. The following tretments were tested: = 4-methoxy cinnmldehyde + indole (florl it in use for WCR served s positive control); = ß-ionone + methyl slicylte + ß-cryophyllene [According to Hmmck et l. (21) this comintion ttrcted more femles thn the known florl WCR it]; = 4-methoxyphenethyl lcohol + cinnmldehyde + (E)- nethole + indole (ttrctnt used for D. reri in North Americ); = phenylcetldehyde + 2-phenylethnol + methyl nthrnylte + eugenol + enzldehyde (compounds shown out from mize silk or tssels in previous studies) = 1-phenylethnol + 3-methyl eugenol + enzyl lcohol + enzyl cette + gerniol (florl compounds showing ttrction to other eetles in previous studies); = united trps served s negtive control

8 In trpping tests femle Femle in ctches in ctch ws eqully high in tretments,, nd Hjdúöszörmény, 25 July 7 - Septemer 19. Totl cught: 1595 eetles 5 Derecen, 25 July 5 - Septemer 19. Totl cught: 6718 eetles men+se c men+se c men+se Pusztzámor, 25 August Totl cught : eetles

9 It ws surprising tht (which hd een Femle in ctch descried s strong femle ttrctnt y US scientists) ws not more ttrctive for femles thn united controls Hjdúöszörmény, 25 July 7 - Septemer 19. Totl cught: 1595 eetles 5 Derecen, 25 July 5 - Septemer 19. Totl cught: 6718 eetles men+se c men+se c men+se Pusztzámor, 25 August Totl cught : eetles

10 Among tretments more Femle ttrctive in for ctch femles, none produced significntly higher femle percentges thn Hjdúöszörmény, 25 July 7 - Septemer 19. Totl cught: 1595 eetles 5 Derecen, 25 July 5 - Septemer 19. Totl cught: 6718 eetles men+se c men+se c men+se Pusztzámor, 25 August Totl cught : eetles

11 Sesonl pttern of femle -s for the two est its suggested slight (ut not convincing) tendency of higher femle rtios in men+se Hjdúöszörmény, 25 July 7 - Septemer A)Dirflorl 19. Totl ctch: D)pheneugenz 9245 eetles Jul 5 Jul 11 Jul 15 Jul 2 Jul 26 Jul 3 Aug 3 Aug 7 Aug 11 Aug 15 Aug 2 Aug 25 Aug 3 Sep 3 Sep 8 Sep 14 Sep 19 Cell Men for FEM(1felett) men+se Derecen, 25 July 5 - Septemer 19. Totl ctch: eetles A)Dirflorl D)pheneugenz Jul 7 Jul 11 Jul 14 Jul 19 Jul 22 Jul 27 Jul 31 Aug 3 Aug 7 Aug 1 Aug 13 Aug 17 Aug 19 Aug 24 Aug 28 Aug 31 Sep 2 Sep 6 Sep 9 Sep 13 Sep 16 Sep 19 Cell Men for FEM(1felett)

12 However, s fr s men Men numers ctches cptured of femles were concerned, cught fr more eetles thn ny of the other tretments. ctch: 6938 eetles ctch: eetles e d d men+se c men+se c men+se Hjdúöszörmény, 25 July 7 - Septemer 19. Totl 25 Derecen, 25 July 5 - Septemer 19. Totl 9 8 Pusztzámor, 25 August Totl ctch: 2655 eetles c

13 If femle ntenne would response etter to given compound, this chemicl would show perspective to e tested for femle-trgeted it Consequently, ntennl responses of WCR were recorded y n EAG pprtus insect ntenn ir flow with stimulus Smple EAG response elicited y methylnthrnilte glss cpillry electrodes 4

14 No difference ws found in EAG response spectr etween mles nd femles no indiction of femle-specific compounds mens of 9 ntenne 2-methyl-1-propnol 2-methyl-1-propnol 2-phenylethnol 2-phenylethnol 4-metoxyphenethyl-lcohol 4-metoxyphenethyl-lcohol nisyl cetone nisyl cetone enzldehyde enzldehyde enzyl cette enzyl cette enzyllcohol enzyllcohol et-ionone et-ionone cinnmyl cette cinnmyl cette cinnmyl OH cinnmyl OH eugenol gerniol i-amyl lcohol isomyl cette isoutyl cette isosfrol lvndulol ir linlool methyl nthrnilte methyl eugenol methylslicylte ethoxy cinnmic ldehyde phenethyl cette phenethyl lcohol (control) phenylcetldehyde trns-nethol trns-cinnmldehyde eugenol gerniol i-amyl lcohol isomyl cette isoutyl cette isosfrol lvndulol levegö linlool methyl nthrnilte methyl eugenol methylslicylte pr-methoxy cinnmic ldehyde phenethyl cette phenethyl lcohol (kontroll) átlg phenylcetldehyde trns-nethol trns-cinnmldehyde (26) men normlized responses (+SE) nisyl cetone enzldehyde enzyl cette enzyl lcohol et-ionone cinnmyl cette cinnmyl lcohol eugenol gerniol isomyl lcohol isomyl cette isoutyl cette isosfrol lvndulol ir linlool methyl nthrnilte methyl eugenol methyl slicylte 4-methoxy cinnmldehyde phenethyl cette phenethyl lcohol phenylcetldehyde trns-nethol trns-cinnmldehyde methyl-1-propnol 2-phenylethnol 4-metoxyphenethyl lcohol control mens of 1 ntenne

15 In conclusion, so fr the most efficient femle-trgeted trpit comintion for WCR is the conventionl florl ttrctnt pplied in KLPflor+ trps. Pls click here to get to our homepge with informtion on trps of other pest insects

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