Millifluidic culture improves human midbrain organoid vitality and differentiation
|
|
- Mervin Garrett
- 5 years ago
- Views:
Transcription
1 Millifluidic culture improves human midbrain organoid vitality and differentiation Emanuel Berger University of Luxembourg
2 3D midbrain organoids for modelling Parkinson s disease Healthy control Midbrain Parkinson s patient Parkinson s disease core symptoms Demands to the human midbrain organoid model: Spatial 3D organisation of heterogeneous midbrain-residing cells Physiological functions of midbrain-specific cells (e.g. dopamine release ) Stable system for extended cultivation and manipulation Patient-specificity Reproducibility Dopaminergic neurons Applications: Disease modelling, Drug testing, Regenerative medicine Double et al. 211
3 3D midbrain organoids Generation Human neuroepithelial stem cells: - Resemble cells of the neural plate border - Are pre-patterned towards mid-/hindbrain identity Protocol developed by A.S. Monzel et al. (Stem Cell Reports. 217) in dependence on Lancaster et al. 213
4 3D midbrain organoids Features Midbrain organoids exhibit neuronal, astroglial and oligodendrocyte differentiation Midbrain specific, due to mda marker expression Midbrain organoids exhibit spatial asymmetry of mda neurons Midbrain organoids are functional Synaptic connections Myelination Electrophysiological activity Monzel AS. et al. Stem Cell Reports. 217
5 3D midbrain organoids Limitations Dead core Shaking Solely shaking does not ensure proper supply High effort of frequent manual media change Susceptible for culture perturbations Need for fluidic culture system Agarose sections
6 Millifluidic culture system Cell culture chamber 6-Chamber Tray Quasi Vivo Peristaltic pump E Berger et al. Lab on a chip (accepted)
7 Reduced dead core size under millifluidic conditions WT T12.9 WT K7 WT A r e a r a tio (d e a d c o r e / to ta l o r g a n o id ) WT T12.9 WT K7 WT o r g a n o id a r e a [m m 2 ] Shaking Fluidic Shaking Fluidic.2 n.s. n.s. n.s. * 2. n.s. n.s. n.s. n.s K 7 W T T W T W T a ll lin e s K 7 W T T W T W T a ll lin e s E Berger et al. Lab on a chip (accepted)
8 Increased oxygenation under millifluidic conditions Shaking Fluidic E Berger et al. Lab on a chip (accepted)
9 Accelerated dopaminergic differentiation K7 WT T12.9 WT WT T H /F O X A 2 b y n u c le a r p e r im e te r T H + F O X A 2 + b y n u c le a r p e rim e te r [p ix e l r a tio ] [p ix e l ra tio, % o f C T R L ] Shaking Fluidic TH/FOXA2/ TUJ1/Hoechst TH TH/FOXA2/ TUJ1/Hoechst TH 1 n.s. n.s. * n.s K 7 W T T W T W T a ll lin e s E Berger et al. Lab on a chip (accepted)
10 Accelerated dopaminergic differentiation K7 WT T12.9 WT WT T H + F O X A 2 + [% o f s in g le c e lls ] T H + /F O X A 2 + [% o f s in g le c e lls ] Shaking Fluidic K 7 W T T W T W T E Berger et al. Lab on a chip (accepted)
11 Accelerated dopaminergic differentiation K7 WT T12.9 WT WT S O X 2 + b y n u c le a r m a s k S O X 2 b y n u c le a r m a s k [p ix e l ra tio, % o f C T R L ] [p ix e l r a tio ] Shaking Fluidic SOX2/Hoechst SOX2 SOX2/Hoechst SOX2 1.5 * n.s. ** * K 7 W T T W T W T a ll lin e s E Berger et al. Lab on a chip (accepted)
12 Metabolic maturation under millifluidic conditions g lu c o s e c o n s u m p tio n la c ta te p r o d u c tio n [m o l/c e ll* d a y ] [m o l/c e ll* d a y ] g lu ta m in e c o n s u m p tio n g lu ta m a te p r o d u c tio n [m o l/c e ll* d a y ] [m o l/c e ll* d a y ] *** ** *** * ** *** D 2 3 D 3 c o m b in e d D 2 3 D 3 c o m b in e d ** * *** *** *** *** D 2 3 D 3 c o m b in e d D 2 3 D 3 c o m b in e d E Berger et al. Lab on a chip (accepted)
13 Summary and outlook Millifluidics improve midbrain organoid cultures in terms of Dead core area Organoid oxygenation Dopaminergic differentiation efficiency Metabolic maturation Practical solution for advanced organoid cultures Quasi Vivo Quasi Brain Further improvement strategies Vascularization Physiological cell density Microglia incorporation disease modeling
14 Acknowledgements LCSB DVB Jens Schwamborn Silvia Bolognin Sarah Nickels Kathrin Hemmer Jonas Walter Javier Jarazo Thea van Wuellen Marie Fossépré Isabel Rosety Philippe Lucarelli Lisa Smits Anna Monzel Gemma Gomez Giro Sonia Sabate Soler Jennifer Modamio Developmental and Cellular Biology Group Collaborators o Chiara Magliaro and Arti Ahluwalia (Università di Pisa) o Silvia Bolognin, Paul Anthony & Anna Monzel (LCSB) o Nicole Paczia & Carole Linster (LCSB Metabolomics core facility)
15 In Vitro Models for the Study of Neurological Diseases Q & A Session Sponsor
Organ-on-a-chip: practical applications & challenges. Remko van Vught
Organ-on-a-chip: practical applications & challenges Remko van Vught 10-4-2018 A leap forward in physiological relevance The challenge of reductionism: Make things as simple as possible, but not simpler,
More informationFRAUNHOFER IME SCREENINGPORT
FRAUNHOFER IME SCREENINGPORT Design of screening projects General remarks Introduction Screening is done to identify new chemical substances against molecular mechanisms of a disease It is a question of
More informationThe Fruit Fly Brain Observatory
The Fruit Fly Brain Observatory Aurel A. Lazar, Columbia University The Columbia University Team: Wesley Bruning Allison L. Fenichel Aurel A. Lazar (PI) Nikul H. Ukani Chung-Heng Yeh Yiyin Zhou The National
More informationMonitoring neurite morphology and synapse formation in primary neurons for neurotoxicity assessments and drug screening
APPLICATION NOTE ArrayScan High Content Platform Monitoring neurite morphology and synapse formation in primary neurons for neurotoxicity assessments and drug screening Suk J. Hong and Richik N. Ghosh
More informationMicrosystems for Neuroscience and Medicine. Lecture 9
1 Microsystems for Neuroscience and Medicine Lecture 9 2 Neural Microsystems Neurons - Structure and behaviour Measuring neural activity Interfacing with neurons Medical applications - DBS, Retinal Implants
More informationFunctional Organization of the Transcriptome in Human Brain
Functional Organization of the Transcriptome in Human Brain Michael C. Oldham Laboratory of Daniel H. Geschwind, UCLA BIOCOMP 08, Las Vegas, NV July 15, 2008 Neurons Astrocytes Oligodendrocytes Microglia
More informationNeural development its all connected
Neural development its all connected How do you build a complex nervous system? How do you build a complex nervous system? 1. Learn how tissue is instructed to become nervous system. Neural induction 2.
More informationScope & Outline. Joanna A. Korecka
Scope & Outline Joanna A. Korecka 10 Scope Parkinson s disease (PD) is a progressive neurodegenerative disorder. According to the Parkinson s Disease Foundation, 7 to 10 million people suffer from PD worldwide.
More informationgender mains treaming in Polis h practice
gender mains treaming in Polis h practice B E R L IN, 1 9-2 1 T H A P R IL, 2 O O 7 Gender mains treaming at national level Parliament 25 % of women in S ejm (Lower Chamber) 16 % of women in S enat (Upper
More informationStochastic Dynamic Causal Modelling for resting-state fmri
Stochastic Dynamic Causal Modelling for resting-state fmri Ged Ridgway, FIL, Wellcome Trust Centre for Neuroimaging, UCL Institute of Neurology, London Overview Connectivity in the brain Introduction to
More informationMeasuring the invisible using Quantitative Magnetic Resonance Imaging
Measuring the invisible using Quantitative Magnetic Resonance Imaging Paul Tofts Emeritus Professor University of Sussex, Brighton, UK Formerly Chair in Imaging Physics, Brighton and Sussex Medical School,
More information2015 U N I V E R S I T I T E K N O L O G I P E T R O N A S
Multi-Modality based Diagnosis: A way forward by Hafeez Ullah Amin Centre for Intelligent Signal and Imaging Research (CISIR) Department of Electrical & Electronic Engineering 2015 U N I V E R S I T I
More informationPhysiology 2 nd year. Neuroscience Optional Lecture
Academic year 2018/2019 Physiology 2 nd year Semester 1 Curricula Nervous system physiology Blood physiology Acid-base equilibrium Bibliography: Boron & Boulpaep Medical Physiology, 3 rd edition Physiology
More informationContinuous cultures in shake flasks
Continuous cultures in shake flasks March 28 2012 Continuous cultures in shake flasks Nordics Bioprocess Improvement Seminar Innovation in cell culture process development & production Stockholm, March
More informationDomain 6: Communication
Domain 6: Communication 6.1: Cell communication processes share common features that reflect a shared evolutionary history. (EK3.D.1) 1. Introduction to Communication Communication requires the generation,
More informationMajor Forty-nine hours required. Minor Eighteen hours required, 100. Science of Life Biological Principles.
BIOLOGY (BIO) Professors Mark Johnson, and Rick Kopp (Chair); Associate Professors Tim Griffith, Tracy Livingston, and Bill Stevens; Visiting Assistant Professor Zsuzsanna Sasvari; Adjuncts Jana Henson,
More informationBEFORE TAKING THIS MODULE YOU MUST ( TAKE BIO-4013Y OR TAKE BIO-
2018/9 - BIO-4001A BIODIVERSITY Autumn Semester, Level 4 module (Maximum 150 Students) Organiser: Dr Harriet Jones Timetable Slot:DD This module explores life on Earth. You will be introduced to the major
More informationDepartment Curriculum and Assessment Outline
Department: Science Year Group: 10 Teaching, learning and assessment during the course: Combined Science 1 2 B1 Key concepts in Biology B2 Cells and control What are the structure and function of cells.
More informationAssessing Human Health Through Real-time Data Collection at the Grand Canyon
Assessing Human Health Through Real-time Data Collection at the Grand Canyon. Principal Investigator: Glory Aviña, PhD MBA Sandia National Laboratories Sandia National Laboratories is a multi-program laboratory
More informationNSAC Isotopes Subcommittee Meeting January 20, 2015 Erin Grady, MD Society of Nuclear Medicine and Molecular Imaging
NSAC Isotopes Subcommittee Meeting January 20, 2015 Erin Grady, MD Society of Nuclear Medicine and Molecular Imaging 1 SNMMI Represents professionals in the field of nuclear medicine Physicians Technologists
More informationPCAT Test Blueprint for
PCAT Subtest Operational Experimental Time Items / Passages Items / Passages Allowed Part 1: Writing 1 Prompt (operational) 30 min. Part 2: Biological Processes (20 30% of items with passages) 40 Items
More informationCourse Descriptions Biology
Course Descriptions Biology BIOL 1010 (F/S) Human Anatomy and Physiology I. An introductory study of the structure and function of the human organ systems including the nervous, sensory, muscular, skeletal,
More informationNuclear Medicine Treatments and Clinical Applications
INAYA MEDICAL COLLEGE (IMC) RAD 243- LECTURE 2 Nuclear Medicine Treatments and Clinical Applications DR. MOHAMMED MOSTAFA EMAM Next Lectures Outlines Introduction to Nuclear Physics Physics of Radioactivity
More informationThe ipro. ipsc-derived cells going high throughput: new strategies for ion channels drug discovery. Silvia Cainarca
The ipro ipsc-derived cells going high throughput: new strategies for ion channels drug discovery Silvia Cainarca Ncardia Applications Workshop - 2017 November 30 th, 2017 Rationale: Early drug discovery,
More informationSelf-assembled Peptides as Drug Delivery Molecules for transport across the Biological Barriers
Self-assembled Peptides as Drug Delivery Molecules for transport across the Biological Barriers Porter, S., McCarthy, H., & Laverty, G. (2017). Self-assembled Peptides as Drug Delivery Molecules for transport
More informationme239 mechanics of the cell - syllabus me239 mechanics of the cell me239 mechanics of the cell - grading me239 mechanics of the cell - overview
6 mechanotransduction wong, goktepe, kuhl [2010] me239 mechanics of the cell add l information http://biomechanics.stanford.edu and coursework 1 me239 mechanics of the cell - syllabus favorite topics in
More informationPrereq: Concurrent 3 CH
0201107 0201101 General Biology (1) General Biology (1) is an introductory course which covers the basics of cell biology in a traditional order, from the structure and function of molecules to the structure
More informationHemolysis and Immune Activation. Karina Yazdanbakhsh, PhD Executive Director, Research Institute New York Blood Center
Hemolysis and Immune Activation Karina Yazdanbakhsh, PhD Executive Director, Research Institute New York Blood Center Hemolysis in Sickle Cell Disease (SCD) Hemolysis activates the underlying endothelium:
More informationDevelopment Plan 2025
Development Plan 2025 4.15 Faculty of Life Sciences 4.15.1 Objectives The Faculty of Life Sciences encompasses the disciplines of biology, pharmacy and nutritional sciences. The Faculty aims at acquiring
More informationHow is it different from conventional MRI? What is MR Spectroscopy? How is it different from conventional MRI? MR Active Nuclei
What is MR Spectroscopy? MR-Spectroscopy (MRS) is a technique to measure the (relative) concentration of certain chemical or biochemical molecules in a target volume. MR-Spectroscopy is an in vivo (in
More informationNeurochemistry 1. Nervous system is made of neurons & glia, as well as other cells. Santiago Ramon y Cajal Nobel Prize 1906
Neurochemistry 1 Nervous system is made of neurons & glia, as well as other cells. Santiago Ramon y Cajal Nobel Prize 1906 How Many Neurons Do We Have? The human brain contains ~86 billion neurons and
More informationKILGORE COLLEGE BIOLOGY DEPARTMENT Biology 2421 Syllabus
COURSE: BIOL 2421 (4-3-4) TITLE: CATALOG DESCRIPTION: Microbiology and Pathology A study of the morphology, physiology, genetics, taxonomy and control of microorganisms. This course includes a study of
More informationIntro and Homeostasis
Intro and Homeostasis Physiology - how the body works. Homeostasis - staying the same. Functional Types of Neurons Sensory (afferent - coming in) neurons: Detects the changes in the body. Informations
More informationThe Role of G-Protein Coupled Estrogen Receptor (GPER) in Early Neurite Development. Kyle Pemberton
The Role of G-Protein Coupled Estrogen Receptor (GPER) in Early Neurite Development Kyle Pemberton Acknowledgement Dr. Xu Lab Members Brittany Mersman Nicki Patel Pallavi Mhaskar Jason Cocjin Committee
More informationIntegrative Biology 200A "PRINCIPLES OF PHYLOGENETICS" Spring 2012 University of California, Berkeley
Integrative Biology 200A "PRINCIPLES OF PHYLOGENETICS" Spring 2012 University of California, Berkeley B.D. Mishler Feb. 7, 2012. Morphological data IV -- ontogeny & structure of plants The last frontier
More informationQuantitative Metrics for White Matter Integrity Based on Diffusion Tensor MRI Data. Stephanie Lee
Quantitative Metrics for White Matter Integrity Based on Diffusion Tensor MRI Data Stephanie Lee May 5, 2005 Quantitative Metrics for White Matter Integrity Based on Diffusion Tensor MRI Data ABSTRACT
More informationREQUIREMENTS FOR THE BIOCHEMISTRY MAJOR
REQUIREMENTS FOR THE BIOCHEMISTRY MAJOR Grade Requirement: All courses required for the Biochemistry major (CH, MATH, PHYS, BI courses) must be graded and passed with a grade of C- or better. Core Chemistry
More informationLassen Community College Course Outline
Lassen Community College Course Outline AGR 20 Introduction to Plant Science 4.0 Units I. Catalog Description This course is an introduction to plant science including structure, growth processes, propagation,
More informationNeuronal Cell Health Assays. Real-time automated measurements of neuronal cell viability and neurite dynamics inside your incubator.
I N C U C Y T E L I V E - C E L L A N A LY S I S S Y S T E M Neuronal Cell Health Assays Real-time automated measurements of neuronal cell viability and neurite dynamics inside your incubator. See what
More informationNeurons, Synapses, and Signaling
CAMPBELL BIOLOGY IN FOCUS URRY CAIN WASSERMAN MINORSKY REECE 37 Neurons, Synapses, and Signaling Lecture Presentations by Kathleen Fitzpatrick and Nicole Tunbridge, Simon Fraser University SECOND EDITION
More informationSTEEL PIPE NIPPLE BLACK AND GALVANIZED
Price Sheet Effective August 09, 2018 Supersedes CWN-218 A Member of The Phoenix Forge Group CapProducts LTD. Phone: 519-482-5000 Fax: 519-482-7728 Toll Free: 800-265-5586 www.capproducts.com www.capitolcamco.com
More informationConclusions. The experimental studies presented in this thesis provide the first molecular insights
C h a p t e r 5 Conclusions 5.1 Summary The experimental studies presented in this thesis provide the first molecular insights into the cellular processes of assembly, and aggregation of neural crest and
More informationResearch Article Analyzing EEG of Quasi-Brain-Death Based on Dynamic Sample Entropy Measures
Computational and Mathematical Methods in Medicine Volume 3, Article ID 68743, 6 pages http://dx.doi.org/.55/3/68743 Research Article Analyzing EEG of Quasi-Brain-Death Based on Dynamic Sample Entropy
More informationAdvanced Anatomy and Physiology
Lakeshore Technical College 10806179 Advanced Anatomy and Physiology Course Outcome Summary Course Information Alternate Title Description Total Credits 4 Total Hours 90 Adv Anatomy & Physiology Advanced
More informationUsing mathematical models & approaches to quantify BRAIN (dynamic) Positron Emission Tomography (PET) data
Using mathematical models & approaches to quantify BRAIN (dynamic) Positron Emission Tomography (PET) data Imaging Seminars Series Stony Brook University, Health Science Center Stony Brook, NY - January
More informationHopfield Neural Network and Associative Memory. Typical Myelinated Vertebrate Motoneuron (Wikipedia) Topic 3 Polymers and Neurons Lecture 5
Hopfield Neural Network and Associative Memory Typical Myelinated Vertebrate Motoneuron (Wikipedia) PHY 411-506 Computational Physics 2 1 Wednesday, March 5 1906 Nobel Prize in Physiology or Medicine.
More information37 Neurons, Synapses, and Signaling
CAMPBELL BIOLOGY IN FOCUS Urry Cain Wasserman Minorsky Jackson Reece 37 Neurons, Synapses, and Signaling Lecture Presentations by Kathleen Fitzpatrick and Nicole Tunbridge Overview: Lines of Communication
More informationGC/MS is not dead yet!!
Complementary Role of GC/MS in Clinical Laboratory and Beyond Uttam Garg, PhD, DABCC, FABFT, FACB Professor, University of Missouri School of Medicine Division Chief, Laboratory Medicine Director, Clinical
More informationOCR (A) Biology A-level
OCR (A) Biology A-level Topic 2.6: Cell division, cell diversity and cellular organisation Notes The role of mitosis and the cell cycle is to produce identical daughter cells for growth and asexual reproduction
More informationWhat are mitochondria?
What are mitochondria? What are mitochondria? An intracellular organelle. There are 100 to 1000s of mitochondria/cell. Most mitochondria come from the mother. Mitochondria have their own DNA Mitochondria
More informationREQUIREMENTS FOR THE BIOCHEMISTRY MAJOR
REQUIREMENTS FOR THE BIOCHEMISTRY MAJOR Grade Requirement: All courses required for the Biochemistry major (CH, MATH, PHYS, BI courses) must be graded and passed with a grade of C- or better. Core Chemistry
More informationChapter 3 BIOLOGY AND BEHAVIOR
Chapter 3 BIOLOGY AND BEHAVIOR Section 1: The Nervous System Section 2: The Brain: Our Control Center Section 3: The Endocrine System Section 4: Heredity: Our Genetic Background 1 Section 1: The Nervous
More informationPlant and animal cells (eukaryotic cells) have a cell membrane, cytoplasm and genetic material enclosed in a nucleus.
4.1 Cell biology Cells are the basic unit of all forms of life. In this section we explore how structural differences between types of cells enables them to perform specific functions within the organism.
More informationIdentification of culturable endophytes isolated from apple tissues with antagonism towards Neonectria ditissima
Identification of culturable endophytes isolated from apple tissues with antagonism towards Neonectria ditissima Jing Liu, Hayley Ridgway & Eirian Jones Background Apple production in NZ widely cultivated
More informationCellular Neuroanatomy II The Prototypical Neuron: Neurites. Reading: BCP Chapter 2
Cellular Neuroanatomy II The Prototypical Neuron: Neurites Reading: BCP Chapter 2 Major Internal Features of a Neuron The neuron is the functional unit of the nervous system. A typical neuron has a soma
More informationBIOLOGY. 1. Overview of Neurons 11/3/2014. Neurons, Synapses, and Signaling. Communication in Neurons
CAMPBELL BIOLOGY TENTH EDITION 48 Reece Urry Cain Wasserman Minorsky Jackson Neurons, Synapses, and Signaling Lecture Presentation by Nicole Tunbridge and Kathleen Fitzpatrick 1. Overview of Neurons Communication
More informationRegulation of Signal Strength by Presynaptic Mechanisms
Regulation of Signal Strength by Presynaptic Mechanisms 9.013 / 7.68: Core Class Sheng Lectures Presynaptic Mechanisms Nathan Wilson Background / Theory Background / Theory The Presynaptic Specialization
More informationComplementary Role of GC/MS in Clinical Laboratory and Beyond
Complementary Role of GC/MS in Clinical Laboratory and Beyond Uttam Garg, PhD, DABCC, FABFT, FACB Professor, University of Missouri School of Medicine Division Chief, Laboratory Medicine Director, Clinical
More informationNovel VLSI Implementation for Triplet-based Spike-Timing Dependent Plasticity
Novel LSI Implementation for Triplet-based Spike-Timing Dependent Plasticity Mostafa Rahimi Azghadi, Omid Kavehei, Said Al-Sarawi, Nicolangelo Iannella, and Derek Abbott Centre for Biomedical Engineering,
More informationBiochemistry-BS Program Study Abroad Pathway
Biochemistry-BS Program Study Abroad Pathway (last revised September 2016) Table 1a: Undergraduate Program Schedule These undergraduate program schedules are subject to change. Please verify information
More informationList of Academic Sessions
List of Academic Sessions Plenary Lectures PL1 Olfaction and Stress 3/28 (Thr) 17:30-18:30 Linda Buck (Fred Hutchinson Cancer Research Center, USA) PL2 Natural Products as Probes of the Pain Pathway: From
More informationwith%dr.%van%buskirk%%%
with%dr.%van%buskirk%%% How$to$do$well?$ Before$class:$read$the$corresponding$chapter$ Come$to$class$ready$to$par9cipate$in$Top$Hat$ Don t$miss$an$exam!!!!!!!!!!!!!!!!!!!!!!!!!!$ But$I m$not$good$with$science
More informationCh 8: Neurons: Cellular and Network Properties, Part 1
Developed by John Gallagher, MS, DVM Ch 8: Neurons: Cellular and Network Properties, Part 1 Objectives: Describe the Cells of the NS Explain the creation and propagation of an electrical signal in a nerve
More informationIn ovo time-lapse analysis after dorsal neural tube ablation shows rerouting of chick hindbrain neural crest
In ovo time-lapse analysis after dorsal neural tube ablation shows rerouting of chick hindbrain neural crest Paul Kulesa, Marianne Bronner-Fraser and Scott Fraser (2000) Presented by Diandra Lucia Background
More informationSuperconductivity in High Energy Particle Accelerators. Peter Schmüser DESY and Institut für Experimentalphysik, Universität Hamburg
Superconductivity in High Energy Particle Accelerators Peter Schmüser DESY and Institut für Experimentalphysik, Universität Hamburg The basics of superconductivity are outlined with special emphasis on
More informationPTM Bioinformatics Yu Xue
PTM Bioinformatics Yu Xue Huazhong University of Science and Technology Wuhan, China Huazhong University of Science and Technology http://www.uniprot.org/docs/ptmlist Post-translational modification POST:
More informationOptional Second Majors
Faculty of Health and Medical Sciences Bachelor of Health Sciences (Advanced) (Pre-2017 Commencement) Optional Second Majors Bachelor of Health Sciences (Advanced) students may choose to complete a second
More informationMuscle Cells skeletal muscle cardiac muscle smooth muscle
Muscle Cells Muscle tissue, one of the four major tissue types, plays the vital role of providing movement and heat generation to the organs of the body. Within muscle tissue are three distinct groups
More informationAnatomy & Physiology Standards and Benchmarks
Anatomy & Standards and Standard 1: Understands and applies principles of scientific inquiry Power : Identifies questions and concepts that guide science investigations Uses technology and mathematics
More informationPET Tracer Kinetic Modeling In Drug
PET Tracer Kinetic Modeling In Drug Discovery Research Applications Sandra M. Sanabria-Bohórquez Imaging Merck & Co., Inc. Positron Emission Tomography - PET PET is an advanced d imaging i technique permitting
More information2182. Hopf bifurcation analysis and control of three-dimensional Prescott neuron model
2182. Hopf bifurcation analysis and control of three-dimensional Prescott neuron model Chunhua Yuan 1, Jiang Wang 2 School of Electrical Engineering and Automation, Tianjin University, Tianjin, China 2
More informationTheoretical Analysis of the Local Field Potential in Deep Brain Stimulation Applications
Theoretical Analysis of the Local Field Potential in Deep Brain Stimulation Applications Scott F. Lempka, Cameron C. McIntyre* Department of Biomedical Engineering, Cleveland Clinic Foundation, Cleveland,
More informationComparison of the expression patterns of several sox genes between Oryzias latipes and Danio rerio
Urun 1 Comparison of the expression patterns of several sox genes between Oryzias latipes and Danio rerio Fatma Rabia URUN ilkent University, nkara - TURKEY High mobility group domain containing transcription
More informationNeurons and Nervous Systems
34 Neurons and Nervous Systems Concept 34.1 Nervous Systems Consist of Neurons and Glia Nervous systems have two categories of cells: Neurons, or nerve cells, are excitable they generate and transmit electrical
More informationBiology September 2015 Exam One FORM G KEY
Biology 251 17 September 2015 Exam One FORM G KEY PRINT YOUR NAME AND ID NUMBER in the space that is provided on the answer sheet, and then blacken the letter boxes below the corresponding letters of your
More informationBiology September 2015 Exam One FORM W KEY
Biology 251 17 September 2015 Exam One FORM W KEY PRINT YOUR NAME AND ID NUMBER in the space that is provided on the answer sheet, and then blacken the letter boxes below the corresponding letters of your
More informationMath 345 Intro to Math Biology Lecture 20: Mathematical model of Neuron conduction
Math 345 Intro to Math Biology Lecture 20: Mathematical model of Neuron conduction Junping Shi College of William and Mary November 8, 2018 Neuron Neurons Neurons are cells in the brain and other subsystems
More informationSCOTCAT Credits: 20 SCQF Level 7 Semester 1 Academic year: 2018/ am, Practical classes one per week pm Mon, Tue, or Wed
Biology (BL) modules BL1101 Biology 1 SCOTCAT Credits: 20 SCQF Level 7 Semester 1 10.00 am; Practical classes one per week 2.00-5.00 pm Mon, Tue, or Wed This module is an introduction to molecular and
More informationNEURONS Excitable cells Therefore, have a RMP Synapse = chemical communication site between neurons, from pre-synaptic release to postsynaptic
NEUROPHYSIOLOGY NOTES L1 WHAT IS NEUROPHYSIOLOGY? NEURONS Excitable cells Therefore, have a RMP Synapse = chemical communication site between neurons, from pre-synaptic release to postsynaptic receptor
More informationNeurons, Synapses, and Signaling
LECTURE PRESENTATIONS For CAMPBELL BIOLOGY, NINTH EDITION Jane B. Reece, Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Robert B. Jackson Chapter 48 Neurons, Synapses, and Signaling
More informationCELL AND MICROBIOLOGY Nadia Iskandarani
7Course Title: Head of Department: Teacher(s) + e-mail: Cycle/Division: Biology IA: CELL AND MICROBIOLOGY Nadia Iskandarani Ms.Ibtessam: ibtissam.h@greenwood.sch.ae High School Grade Level: Grade 10 Credit
More informationCOGNITIVE SCIENCE 107A
COGNITIVE SCIENCE 107A Electrophysiology: Electrotonic Properties 2 Jaime A. Pineda, Ph.D. The Model Neuron Lab Your PC/CSB115 http://cogsci.ucsd.edu/~pineda/cogs107a/index.html Labs - Electrophysiology
More informationNETWORK BIOLOGY AND COMPLEX DISEASES. Ahto Salumets
NETWORK BIOLOGY AND COMPLEX DISEASES Ahto Salumets CENTRAL DOGMA OF BIOLOGY https://en.wikipedia.org/wiki/central_dogma_of_molecular_biology http://www.qaraqalpaq.com/genetics.html CHROMOSOMES SINGLE-NUCLEOTIDE
More informationTEST SUMMARY AND FRAMEWORK TEST SUMMARY
Washington Educator Skills Tests Endorsements (WEST E) TEST SUMMARY AND FRAMEWORK TEST SUMMARY BIOLOGY Copyright 2014 by the Washington Professional Educator Standards Board 1 Washington Educator Skills
More informationc. What is the average rate of change of f on the interval [, ]? Answer: d. What is a local minimum value of f? Answer: 5 e. On what interval(s) is f
Essential Skills Chapter f ( x + h) f ( x ). Simplifying the difference quotient Section. h f ( x + h) f ( x ) Example: For f ( x) = 4x 4 x, find and simplify completely. h Answer: 4 8x 4 h. Finding the
More information9.01 Introduction to Neuroscience Fall 2007
MIT OpenCourseWare http://ocw.mit.edu 9.01 Introduction to Neuroscience Fall 2007 For information about citing these materials or our Terms of Use, visit: http://ocw.mit.edu/terms. 9.01 Recitation (R02)
More informationCells. Steven McLoon Department of Neuroscience University of Minnesota
Cells Steven McLoon Department of Neuroscience University of Minnesota 1 Microscopy Methods of histology: Treat the tissue with a preservative (e.g. formaldehyde). Dissect the region of interest. Embed
More information! Depolarization continued. AP Biology. " The final phase of a local action
! Resting State Resting potential is maintained mainly by non-gated K channels which allow K to diffuse out! Voltage-gated ion K and channels along axon are closed! Depolarization A stimulus causes channels
More informationAdvances in particle concentration measurements
Advances in particle concentration measurements Dr Hanna Jankevics Jones Principal Applications Scientist Company Confidential Outline Advances in particle concentration from Malvern Panalytical Capture
More informationDendrites - receives information from other neuron cells - input receivers.
The Nerve Tissue Neuron - the nerve cell Dendrites - receives information from other neuron cells - input receivers. Cell body - includes usual parts of the organelles of a cell (nucleus, mitochondria)
More informationYear: Subject Area: Biochemistry, Genetics and Molecular Biology. Subject Category: Aging.
Year: 2011. Subject Category: Aging. 1 Aging Cell 2 Ageing Research Reviews 3 Psychology and Aging 4 Journals of Gerontology - Series A Biological Sciences and Medical Sciences 5 Mechanisms of Ageing and
More informationCells of the nervous system
Cells of the nervous system There are approximately 100 billion neurons in the human brain There are about 100 times as many glial cells in the human brain Similar origin, different functions Other cells
More informationPhoto. EPRI s Power System and Railroad Electromagnetic Compatibility Handbook
Photo EPRI s Power System and Railroad Electromagnetic Compatibility Handbook Brian Cramer Project Manager Transmission and Substations bcramer@epri.com 815/478-5344 Problem Periodic false activation of
More informationGuide to the Science Area For History and Science Concentrators Science and Society Track
Guide to the Science Area For History and Science Concentrators Science and Society Track 2017-2018 Department of the History of Science This guide provides ideas for designing your own program of study
More informationEKOLOGIE EN SYSTEMATIEK. T h is p a p e r n o t to be c i t e d w ith o u t p r i o r r e f e r e n c e to th e a u th o r. PRIMARY PRODUCTIVITY.
EKOLOGIE EN SYSTEMATIEK Ç.I.P.S. MATHEMATICAL MODEL OF THE POLLUTION IN NORT H SEA. TECHNICAL REPORT 1971/O : B i o l. I T h is p a p e r n o t to be c i t e d w ith o u t p r i o r r e f e r e n c e to
More informationThe Nervous System. What did you learn at school today? Neurophysiology!
The Nervous System What did you learn at school today? Neurophysiology! The Nervous System Controls heart rate, emotions, memories, consciousness, and much more. The most intricate and beautifully complex
More informationMolecular and cellular biology is about studying cell structure and function
Chapter 1 Exploring the World of the Cell In This Chapter Discovering the microscopic world Getting matter and energy Reading the genetic code Molecular and cellular biology is about studying cell structure
More informationToxicokinetics: Absorption, Distribution, and Excretion. Michael A. Trush, PhD Johns Hopkins University
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike License. Your use of this material constitutes acceptance of that license and the conditions of use of materials on this
More informationFinnigan LCQ Advantage MAX
www.ietltd.com Proudly serving laboratories worldwide since 1979 CALL +847.913.0777 for Refurbished & Certified Lab Equipment Finnigan LCQ Advantage MAX The Finnigan LCQ Advantage MAX ion trap mass spectrometer
More informationadaptation a structure, feature, or behavior that helps an organism survive and/or reproduce (IG)
FOSS Living Systems Module Glossary 3 rd Edition 2012 adaptation a structure, feature, or behavior that helps an organism survive and/or reproduce (IG) algae a large plantlike group of water organisms
More information