KILGORE COLLEGE BIOLOGY DEPARTMENT Biology 2421 Syllabus

Transcription

1 COURSE: BIOL 2421 (4-3-4) TITLE: CATALOG DESCRIPTION: Microbiology and Pathology A study of the morphology, physiology, genetics, taxonomy and control of microorganisms. This course includes a study of the roles of microorganisms in science and disease and requires the identification of unknowns in mixed microbial cultures. CREDIT HOURS: Four (4) PREREQUISITE: INSTRUCTOR: COURSE RATIONALE: EDUCATIONAL MATERIALS: EVALUATION: C or better in Biol The student must have passed all sections of the THEA test and/or completed all reading, writing, and math requirements or be taking Math 0308 concurrently. Laboratory fee $20. F, Sp, Su ( ). Dr. Tom DeLany, PhD Debbie Williams This course meets the requirements for the Kilgore College Microbiology and Pathology requirement for the associate degree allied health programs, and bachelor degree programs in nursing. Textbook: Wiley, Sherwood, and Wolverton Prescott s Microbiology, 8th Edition. McGraw-Hill Publishers: New York, NY. Laboratory Manual: Leboffe and Pierce Microbiology Laboratory Theory & Application. Morton Publishing Company: Englewood, Colorado. Supplements: Instructors course web site. Laboratory equipment, supplies, and handouts. Lecture: 50% of final grade 4 Major Exams 1 Quiz Grade (average of quiz grades) Departmental comprehensive exam Laboratory: 50% of final grade 3 Major Practical Exams Unknowns 1 Quiz Grade (average of quiz grades) Aseptic Technique Test Microscope Test

2 CLASSROOM POLICIES: DISCLAIMER: COMMON COURSE OUTCOMES: A. Bring writing paper, pencils or pens, and textbooks to each class. B. Do not read novels, other textbooks, school newspapers, etc. in class. C. Be on time and stay in class or lab the entire period. D. Do not miss lecture or lab exams. Make-up day for missed exams will be determined by the individual instructor. E. Daily grades cannot be made up. F. Anyone caught cheating on any work for this class will be immediately dropped from the course with an F. G. If you come to lecture/lab to take a quiz, land then leave, the quiz grade is a zero. H. All electronic equipment must be turned off and out of sight unless allowed by the instructor. I. Attendance: As stated in the Kilgore College Student Handbook. J. Children are not allowed in lectures or in labs. Your instructor reserves the right to make modifications in content and schedule as necessary to promote the best education possible within prevailing conditions affecting this course. A. Students will explain historically important contributions to medicine and other areas of biology made by researchers in the course of the development of microbiology. EEO: (A,B,C) B. Students will explain the structure of the Prokaryotic cell and compare and contrast it to that of the Eukaryotic cell. EEO: (B, C) C. Students will compare and contrast metabolic processes utilized by microorganisms in energy generation, and demonstrate & apply that knowledge in the interpretation of biochemical tests. EEO: (B,D) D. Students will differentiate between the nutritional types of microorganisms and the various methods employed in the control of microorganisms. EEO: (A,B,D) E. Students will compare and contrast the central dogma in Prokaryotes and Eukaryotes, and mechanisms of genetic exchange in Prokaryotes. EEO: (B,C) F. Students will describe the cellular morphology and important staining and cultural characteristics of members of representative genera of the major groups of bacteria, and practice and apply diagnostic techniques for determining such characteristics. EEO: (B,C,D)

3 COMMON COURSE OUTCOMES (continued): G. Students will describe and identify the causative agents and important characteristics of human diseases caused by viruses, bacteria, fungi, and protozoans. EEO: (A,C,D) H. Students will differentiate and describe the general characteristics of representative families of animal viruses, bacteriophages, fungi, and protozoans including their taxonomy, structure, and modes of reproduction. EEO: (C,D) I. Students will demonstrate knowledge of and practice proper techniques in the use and care of the microscope and other laboratory equipment. EEO: (C,D) J. Students will perform basic aseptic microbiological techniques and apply these in the isolation of bacteria from mixed culture and maintenance of these organisms in pure culture. EEO: (C,D) K. Students will perform basic techniques for the quantification of bacteria in culture. EEO: (C,D) L. Students will perform and interpret enzyme assays, and differential and selective media tests for the purpose of identifying bacteria. EEO: (C,D) Exemplary Educational Objectives (EO) are in the Faculty Handbook or on the Kilgore College web-site. SCHEDULE OF CLASSES AND/OR LABS: Three clock hours per week for lecture and four clock hours per week for laboratory. See attached scheduled.

4 Tentative Lecture Schedule The Student Must Read Assigned Chapters Before Class Chapter 1 Chapter 2 Chapter 3 Chapter 4 Chapter 5 Chapter 6 Chapter 8 Chapter 9 Chapter 11 Chapter 13 Chapter 19 Chapter 38 Chapter The History and Scope of Microbiology The study of Microbial Structure: Microscopy and Specimen Preparation Prokaryotic Cell Structure & Function Eukaryotic Cell Structure & Function (review comparison of Prokaryotic & Eukaryotic cells and Euk organelles only). Microbial Nutrition (to be done outside of class with study guide) Lecture Exam 1 Microbial Growth (to be done outside of class with study guide) Metabolism: Energy, Enzymes, and Regulation (select parts) Metabolism: Energy Release and Conservation Microbial Genetics: Gene Structure, Replication, and Expression Microbial Genetics: Mechanisms of Genetic Variation (mutations- 13.1) Lecture Exam 2 Microbial Evolution, Taxonomy, and Diversity Human Diseases Caused by Bacteria Taxonomic Survey Chapters ( will cover only select parts along with the Diseases, i.e. characteristics of streptococci with streptococcal diseases) Lecture Exam 3 Chapter 7 Controls of Microorganisms (to be done outside of class with study guide) Chapter 16 The Viruses: Intro & General Characteristics Chapter 17 The Viruses: Viruses of Bacteria and Archaea (select parts) Chapter 18 The Viruses: Eukaryotic Viruses and other Acellular Infectious Agents Chapter 37 Human Diseases Caused by Viruses and Prions Chapter 39 Lecture Exam 4 Human Diseases Caused by Fungi & Protists ( to be done outside of class with study guide) Comprehensive Final Exam

5 Tentative Lab Schedule The Student Must Read Assigned Exercise Before Lab Class Session# Exercise# Subject Content of Exercises The Compound Microscope Aseptic Transfer Techniques ,3.6...Simple & Gram Stains 4...Simple & Gram Stains (contd) ,3.9...Acid-Fast & Endospore Stains 6...Acid-Fast & Endospore Stains (contd) Ubiquity, Culture Characteristics, Colony Morphology 8 1.4,1.6,3.10,5.22 Streak & Spread Plates, Wet Mounts & 9 LAB PRACTICAL 1...Covers Sessions ,6.1,handout.Quantifying Bacteria 11 Quantifying Bacteria (contd) Antibiotic Sensitivity , Catalase, Oxidase, Nitrate red., Starch Hydro. 14 Enzyme assays (contd) ,5.14,5.21,handout.Urea/Gelatin hydro., Coagulase, Cyst. Desulf. 16 Enzyme assays (contd) ,5.18,handout.Oxidation-Fermentation (OF Glucose) Phenol Red Carbohydrate fermentation Kliger Iron/Triple Sugar Iron agar (TSI) OF Glucose, Phenol red, TSI (contd) 19.ASEPTIC TECHNIQUE, MICROSCOPE TESTS 20.LAB PRACTICAL 2..Covers Sessions ,5.7,5.17*,handout Indole, Methyl Red, Voges-Proskauer, Citrate 22..*indole section only..imvic (contd) ,4.6,5.20,handout...Selective & Differential Media 24...Selective & Differential Media (contd) 25...LAB PRACTICAL 3..Covers Sessions to 30...Isolation of Unknowns from Mixed Cultures handouts, , Bergey s Manual (reference) ** Report sheets and Daily log records for Unknowns are due at the end of the last lab class. If you do not correctly identify your organisms you may have a 2nd try, but with a deduction of 20 points. No 3rd attempt will be allowed.