Nonlinear Optics. Single-Molecule Microscopy Group. Physical Optics Maria Dienerowitz.
|
|
|
- Deborah Gilbert
- 5 years ago
- Views:
Transcription
1 Single-Molecule Microscopy Group Nonlinear Optics Physical Optics Maria Dienerowitz
2 Contents Introduction light matter interactions nonlinear optical susceptibility phase matching Nonlinear optics in microscopy parametric vs non-parametric 2 nd harmonic generation (SHG) two photon excitation fluorescence (TPEF) osteoarthritic cartilage imaging 3 rd harmonic generation (THG) cell lineage reconstruction of early zebrafish embryos stimulated Raman scattering (SRS) coherent anti-stokes Raman scattering (CARS) 2
3 Nonlinear Optics nonlinear response cell division linear response Hooke s law 3
4 Classification of optical media metals n>0 dielectric optical frequencies n<0 negative-index materials? refractive index 4
5 Speed of light in matter vacuum speed of light vacuum permittivity vacuum permeability speed of light in matter refractive index relative permittivity relative permeability 5
6 Material parameters permittivity relative permittivity electric susceptibility 6
7 Constitutive equations response of bound charge and current to applied field polarisation density displacement field 7
8 Nonlinear material response electric dipole moment polarisation density linear polarisation 2 nd order nonlinear polarisation 3 rd order nonlinear polarisation 8
9 Nonlinear material response linear optical susceptibility 2 nd order nonlinear optical susceptibility 3 rd order nonlinear optical susceptibility atomic fields laser light -> 1mW W sun light 9
10 Nonlinear material response 10
11 Inversion symmetry inversion symmetry = centrosymmetric no inversion symmetry = non-centrosymmetric 11
12 Nonlinear material response inversion-symmetric materials (gases, glasses, fluids, benzene (C 6 H 6 ), ) materials with and without inversion symmetry second-harmonic generation sum-frequency generation difference-frequency generation optical parametric oscillation third-harmonic generation self-focussing saturable absorption two-photon absorption stimulated Raman scattering 4-wave mixing (CARS) 12
13 Time-varying polarisation source term wave equation in nonlinear optical media example: second harmonic generation electric field 2 nd order polarisation optical rectification 2 nd harmonic frequency radiation 13
14 2 nd order interactions electric field 2 nd order polarisation second harmonic generation (SHG) sum frequency generation (SFG) difference frequency generation (DFG) optical rectification (OR) 14
15 3 rd order interactions electric field 3 rd order polarisation contains 22 different frequency components (and the negative of each): 15
16 Phase matching frequency matching condition phase matching condition SHG SFG SHG SFG spatial phase matching temporal phase matching destructive interference 16
17 Phase matching SHG wavenumber dispersion same material different frequency normal dispersive material birefringent uniaxial crystal Adrian Pingstone 17
18 Birefringent phase matching non critical phase matching temperature matching coherence length momentum mismatch 18
19 Birefringent phase matching critical phase matching angle matching extraordinary rays: -> beam walkoff 19
20 Summary theoretical foundation source term frequency matching phase matching birefringence 20
21 Contents Introduction light matter interactions nonlinear optical susceptibility phase matching Nonlinear optics in microscopy parametric vs non-parametric 2 nd harmonic generation (SHG) two photon excitation fluorescence (TPEF) osteoarthritic cartilage imaging 3 rd harmonic generation (THG) cell lineage reconstruction of early zebrafish embryos stimulated Raman scattering (SRS) coherent anti-stokes Raman scattering (CARS) 21
22 Quantitative cellular imaging fluorescent staining - site specificity - altering physical or physiological properties bovine pulmonary artery endothelial cell green: F-actin (Alexa Fluor 488) red: blue: mitochondria (MitoTrackerRed) nuclei (DAPI) Justass, Wikipedia 22
23 Nonlinear optics in microscopy - noninvasive - intrinsic and often specific chemical contrast second harmonic generation (SHG) -> non-centrosymmetric structures third harmonic generation (THG) -> optical heterogeneities (interface) microtubule in acute brain slice of 8-month-old wild-type mouse Drosophila embryo, lipid droplets imaged by THG A Kwan et al., PNAS 105, (2008), D Debarre et al., Optics Letters 29, 2881 (2004) 23
24 Parametric vs non-parametric parametric process non-parametric process -> no net energy transfer -> involves energy loss (or gain) duration of virtual state real susceptibility complex susceptibility complex index of refraction absorption 24
25 Parametric vs non-parametric parametric process non-parametric process second harmonic generation (SHG) two photon excitation fluorescence (TPEF) sum frequency generation (SFG) third harmonic generation (THG) stimulated Raman scattering (SRS) coherent anti-stokes Raman scattering (CARS) 25
26 Second harmonic generation - SHG process, non-centrosymmetric material -> collagen -> microtubules -> muscle myosin 2 nd harmonic generation sum frequency generation - SFG small excitation volume decreased background deeper imaging because of longer λ exc 26
27 Two photon excitation fluorescence - TPEF 1 photon 2 photon less photobleaching arrive with s (1 attosecond) E < 2E 1 imaging depth: -> confocal laser scanning 50-80μm -> multi-photon imaging μm 27
28 SHG microscopy of early stage osteoarthritic cartilage characterisation of extra-cellular cartilage matrix -> detection and understanding of progression of Osteoarthritis SHG 5μm SHG 15μm SHG 30μm TPEF 30μm SHG 3D TPEF 3D R Kumar et al., Biomedical Optics Express 6, 1895 (2015) 28
29 Third harmonic generation - THG interface -> cell surface, intercellular lipid droplets no molecular asymmetry required 29
30 Cell Lineage Reconstruction of Early Zebrafish Embryos spherical sample -> deep imaging: 400μm penetration depth observe development in real time -> minute-temporal resolution high resolution microscopy -> 1μm spatial resolution TPEF + SHG + THG N Olivier et al., Science 329, 967 (2010) 30
31 Structural imaging with THG animal pole λ exc =1200nm vegetal pole 100μm N Olivier et al., Science 329, 967 (2010) zebrafish embryo from the one-cell stage 31
32 Comparison structural detail imaged by THG and TPEF 100μm THG signal TPEF membrane staining N Olivier et al., Science 329, 967 (2010) 32
33 Nuclear envelope fragmentation revealed by THG 50μm nuclear envelope dynamics during mitosis N Olivier et al., Science 329, 967 (2010) 33
34 Mitotic spindle imaging with SHG SHG mitotic spindle -> microtubules present during mitosis TPEF H2B/mCherry fusion protein -> chromatin condensation, chromosome formation and displacements N Olivier et al., Science 329, 967 (2010) 34
35 Mitosis steps revealed by TPEF THG SHG microscopy TPEF β-actin THG cell contour SHG mitotic spindle overlay N Olivier et al., Science 329, 967 (2010) 35
36 Raman Scattering linear optics non-parameteric process parameteric process 36
37 Raman Scattering nonlinear optics non-parameteric process parameteric process Stimulated Raman scattering (SRS) Coherent Anti-Stokes Raman scattering (CARS) 37
Nonlinear Optics. Single-Molecule Microscopy Group. Physical Optics Maria Dienerowitz.
Single-Molecule Microscopy Group Nonlinear Optics Physical Optics 21-06-2017 Maria Dienerowitz maria.dienerowitz@med.uni-jena.de www.single-molecule-microscopy.uniklinikum-jena.de Contents Introduction
Multiphoton Imaging and Spectroscopy in Cell and Tissue Biophysics. J Moger and C P Winlove
Multiphoton Imaging and Spectroscopy in Cell and Tissue Biophysics J Moger and C P Winlove Relating Structure to Function Biochemistry Raman microspectrometry Surface enhanced Raman spectrometry (SERS)
Multiphoton microscopy
Multiphoton microscopy Joonas Holmi ELEC October 6, 2016 Multiphoton microscopy 1. 2. 3. 4. Multiphoton microscopy 2/14 Intro: Multiphoton microscopy Nonlinear optical characterization method Pulsed laser
requency generation spectroscopy Rahul N
requency generation spectroscopy Rahul N 2-11-2013 Sum frequency generation spectroscopy Sum frequency generation spectroscopy (SFG) is a technique used to analyze surfaces and interfaces. SFG was first
Nanomaterials and their Optical Applications
Nanomaterials and their Optical Applications Winter Semester 2013 Lecture 02 rachel.grange@uni-jena.de http://www.iap.uni-jena.de/multiphoton Lecture 2: outline 2 Introduction to Nanophotonics Theoretical
Introduction to Nonlinear Optics
Introduction to Nonlinear Optics Prof. Cleber R. Mendonca http://www.fotonica.ifsc.usp.br Outline Linear optics Introduction to nonlinear optics Second order nonlinearities Third order nonlinearities Two-photon
12. Nonlinear optics I
1. Nonlinear optics I What are nonlinear-optical effects and why do they occur? Maxwell's equations in a medium Nonlinear-optical media Second-harmonic generation Conservation laws for photons ("Phasematching")
2.4 Properties of the nonlinear susceptibilities
2.4 Properties of the nonlinear susceptibilities 2.4.1 Physical fields are real 2.4.2 Permutation symmetry numbering 1 to n arbitrary use symmetric definition 1 2.4.3 Symmetry for lossless media two additional
Optical and Photonic Glasses. Lecture 37. Non-Linear Optical Glasses I - Fundamentals. Professor Rui Almeida
Optical and Photonic Glasses : Non-Linear Optical Glasses I - Fundamentals Professor Rui Almeida International Materials Institute For New Functionality in Glass Lehigh University Non-linear optical glasses
The Design and Construction of a. Second Harmonic Generation Microscope. For Collagen Imaging
The Design and Construction of a Second Harmonic Generation Microscope For Collagen Imaging A thesis submitted in partial fulfillment of the requirements for the Degree of Master of Science in Medical
Analysis of second-harmonic generation microscopy under refractive index mismatch
Vol 16 No 11, November 27 c 27 Chin. Phys. Soc. 19-1963/27/16(11/3285-5 Chinese Physics and IOP Publishing Ltd Analysis of second-harmonic generation microscopy under refractive index mismatch Wang Xiang-Hui(
Nonlinear Effects in Optical Fiber. Dr. Mohammad Faisal Assistant Professor Dept. of EEE, BUET
Nonlinear Effects in Optical Fiber Dr. Mohammad Faisal Assistant Professor Dept. of EEE, BUET Fiber Nonlinearities The response of any dielectric material to the light becomes nonlinear for intense electromagnetic
χ (3) Microscopic Techniques
χ (3) Microscopic Techniques Quan Wang Optical Science and Engineering University of New Mexico Albuquerque, NM 87131 Microscopic techniques that utilize the third order non-linearality (χ (3) ) of the
Towards Nonlinear Endoscopes
Towards Nonlinear Endoscopes Hervé Rigneault Institut Fresnel Marseille Nonlinear optical contrast mechanisms: label free imaging ω S ω S ω P ω P TPEF SHG THG Raman CARS SRS ω P ω S = Ω R CARS SRS Molecular
Lukas Gallmann. ETH Zurich, Physics Department, Switzerland Chapter 4b: χ (2) -nonlinearities with ultrashort pulses.
Ultrafast Laser Physics Lukas Gallmann ETH Zurich, Physics Department, Switzerland www.ulp.ethz.ch Chapter 4b: χ (2) -nonlinearities with ultrashort pulses Ultrafast Laser Physics ETH Zurich Contents Second
NONLINEAR OPTICS. Ch. 1 INTRODUCTION TO NONLINEAR OPTICS
NONLINEAR OPTICS Ch. 1 INTRODUCTION TO NONLINEAR OPTICS Nonlinear regime - Order of magnitude Origin of the nonlinearities - Induced Dipole and Polarization - Description of the classical anharmonic oscillator
What happens when light falls on a material? Transmission Reflection Absorption Luminescence. Elastic Scattering Inelastic Scattering
Raman Spectroscopy What happens when light falls on a material? Transmission Reflection Absorption Luminescence Elastic Scattering Inelastic Scattering Raman, Fluorescence and IR Scattering Absorption
(Introduction) Linear Optics and Nonlinear Optics
18. Electro-optics (Introduction) Linear Optics and Nonlinear Optics Linear Optics The optical properties, such as the refractive index and the absorption coefficient are independent of light intensity.
Nanoscale optical circuits: controlling light using localized surface plasmon resonances
Nanoscale optical circuits: controlling light using localized surface plasmon resonances T. J. Davis, D. E. Gómez and K. C. Vernon CSIRO Materials Science and Engineering Localized surface plasmon (LSP)
Solution set for EXAM IN TFY4265/FY8906 Biophysical microtechniques
ENGLISH NORWEGIAN UNIVERSITY OF SCIENCE AND TECHNOLOGY DEPARTMENT OF PHYSICS Contact during exam: Magnus Borstad Lilledahl Telefon: 73591873 (office) 92851014 (mobile) Solution set for EXAM IN TFY4265/FY8906
Introduction to nonlinear optical spectroscopy
Introduction to nonlinear optical spectroscopy Eric Vauthey Département de Chimie-Physique de l Université de Genève, 30 Quai Ernest Ansermet, CH-1211 Genève 4 ( http://www.unige.ch/sciences/chifi/vauthey
We are IntechOpen, the world s leading publisher of Open Access books Built by scientists, for scientists. International authors and editors
We are IntechOpen, the world s leading publisher of Open Access books Built by scientists, for scientists 3,800 116,000 120M Open access books available International authors and editors Downloads Our
Nonlinear Optics. Second Editio n. Robert W. Boyd
Nonlinear Optics Second Editio n Robert W. Boyd Preface to the Second Edition Preface to the First Edition xiii xv 1. The Nonlinear Optical Susceptibility 1 1.1. Introduction to Nonlinear Optics 1 1.2.
Non-linear Optics III (Phase-matching & frequency conversion)
Non-linear Optics III (Phase-matching & frequency conversion) P.E.G. Baird MT 011 Phase matching In lecture, equation gave an expression for the intensity of the second harmonic generated in a non-centrosymmetric
Sources supercontinuum visibles à base de fibres optiques microstructurées
Sources supercontinuum visibles à base de fibres optiques microstructurées P. Leproux XLIM - Université de Limoges Journées Thématiques CMDO+, Palaiseau, 24-25 nov. 2008 Palaiseau, 25/11/2008 - P. Leproux
Module 4 : Third order nonlinear optical processes. Lecture 28 : Inelastic Scattering Processes. Objectives
Module 4 : Third order nonlinear optical processes Lecture 28 : Inelastic Scattering Processes Objectives In this lecture you will learn the following Light scattering- elastic and inelastic-processes,
Advanced Vitreous State The Physical Properties of Glass
Advanced Vitreous State The Physical Properties of Glass Active Optical Properties of Glass Lecture 20: Nonlinear Optics in Glass-Fundamentals Denise Krol Department of Applied Science University of California,
Material Analysis. What do you want to know about your sample? How do you intend to do for obtaining the desired information from your sample?
Material Analysis What do you want to know about your sample? How do you intend to do for obtaining the desired information from your sample? Why can you acquire the proper information? Symmetrical stretching
Chem 442 Review of Spectroscopy
Chem 44 Review of Spectroscopy General spectroscopy Wavelength (nm), frequency (s -1 ), wavenumber (cm -1 ) Frequency (s -1 ): n= c l Wavenumbers (cm -1 ): n =1 l Chart of photon energies and spectroscopies
INTERNATIONAL SCHOOL OF PHYSICS ENRICO FERMI MICROSCOPY APPLIED TO BIOPHOTONICS Varenna (Lake Como) - July 12th to 22nd 2011
INTERNATIONAL SCHOOL OF PHYSICS ENRICO FERMI MICROSCOPY APPLIED TO BIOPHOTONICS Varenna (Lake Como) - July 12th to 22nd 2011 Basic multiphoton and SHG microscopy Peter T. C. So Department of Mechanical
Grading. Class attendance: (1 point/class) x 9 classes = 9 points maximum Homework: (10 points/hw) x 3 HW = 30 points maximum
Grading Class attendance: (1 point/class) x 9 classes = 9 points maximum Homework: (10 points/hw) x 3 HW = 30 points maximum Maximum total = 39 points Pass if total >= 20 points Fail if total
Introduction to non linear optical microscopy
MicroCARS: Chemical Imaging by means of CARS microscopy Introduction to non linear optical microscopy Herve Rigneault Institut Fresnel, Mosaic group, Marseille, France Outline Principles of non-linear
Vibrational imaging and microspectroscopies based on coherent anti-stokes Raman scattering (CARS)
Vibrational imaging and microspectroscopies based on coherent anti-stokes Raman scattering (CARS) by Andreas Volkmer Universität Stuttgart 3 rd Institute of Physics, University of Stuttgart, Pfaffenwaldring
8. Propagation in Nonlinear Media
8. Propagation in Nonlinear Media 8.. Microscopic Description of Nonlinearity. 8... Anharmonic Oscillator. Use Lorentz model (electrons on a spring) but with nonlinear response, or anharmonic spring d
Multiple Choice Identify the letter of the choice that best completes the statement or answers the question.
chapter 7 Test Multiple Choice Identify the letter of the choice that best completes the statement or answers the question. 1. Who was one of the first people to identify and see cork cells? a. Anton van
Applications à la microscopie non-linéaire
Applications à la microscopie non-linéaire Sophie Brasselet Institut Fresnel, Marseille sophie.brasselet@fresnel.fr Nonlinear Microscopy and Pulse Shaping 100µm In vivo mouse spinal cord: Myelin (CARS)
Lasers and Electro-optics
Lasers and Electro-optics Second Edition CHRISTOPHER C. DAVIS University of Maryland III ^0 CAMBRIDGE UNIVERSITY PRESS Preface to the Second Edition page xv 1 Electromagnetic waves, light, and lasers 1
Rapid hyperspectral, vibrationally resonant sum-frequency generation microscopy
Rapid hyperspectral, vibrationally resonant sum-frequency generation microscopy Adam M. Hanninen a and Eric O. Potma b a Department of Astronomy and Physics, University of California, Irvine, CA 92697,
4. The interaction of light with matter
4. The interaction of light with matter The propagation of light through chemical materials is described by a wave equation similar to the one that describes light travel in a vacuum (free space). Again,
An Introduction to Diffraction and Scattering. School of Chemistry The University of Sydney
An Introduction to Diffraction and Scattering Brendan J. Kennedy School of Chemistry The University of Sydney 1) Strong forces 2) Weak forces Types of Forces 3) Electromagnetic forces 4) Gravity Types
IR Spectrography - Absorption. Raman Spectrography - Scattering. n 0 n M - Raman n 0 - Rayleigh
RAMAN SPECTROSCOPY Scattering Mid-IR and NIR require absorption of radiation from a ground level to an excited state, requires matching of radiation from source with difference in energy states. Raman
16. More About Polarization
16. More About Polarization Polarization control Wave plates Circular polarizers Reflection & polarization Scattering & polarization Birefringent materials have more than one refractive index A special
Physics of Light and Optics
Physics of Light and Optics Justin Peatross and Harold Stokes Brigham Young University Department of Physics and Astronomy All Publication Rights Reserved (2001) Revised April 2002 This project is supported
Laser Tweezers and Other Advanced Physical Methods
Phys 6715 - Biomedical Physics Laser Tweezers and Other Advanced Physical Methods Yong-qing Li, PhD Department of Physics, East Carolina University Greenville, NC 27858, USA Email: liy@ecu.edu 1 Optical
Advanced Microscopy. Wintersemester 2012/13 Alexander Heisterkamp Nonlinear Microscopy
Advanced Microscopy Wintersemester 2012/13 Alexander Heisterkamp Nonlinear Microscopy 07.01.2013 Contact Prof.&Dr.&Alexander&Heisterkamp& Friedrich9Schiller9Universität&Jena& Ins@tute&für&Angewandte&Op@k&
Advanced Spectroscopy Laboratory
Advanced Spectroscopy Laboratory - Raman Spectroscopy - Emission Spectroscopy - Absorption Spectroscopy - Raman Microscopy - Hyperspectral Imaging Spectroscopy FERGIELAB TM Raman Spectroscopy Absorption
Reading Assignments. A. Systems of Cell Division. Lecture Series 5 Cell Cycle & Cell Division
Lecture Series 5 Cell Cycle & Cell Division Reading Assignments Read Chapter 18 Cell Cycle & Cell Death Read Chapter 19 Cell Division Read Chapter 20 pages 659-672 672 only (Benefits of Sex & Meiosis sections)
Lecture Series 5 Cell Cycle & Cell Division
Lecture Series 5 Cell Cycle & Cell Division Reading Assignments Read Chapter 18 Cell Cycle & Cell Death Read Chapter 19 Cell Division Read Chapter 20 pages 659-672 672 only (Benefits of Sex & Meiosis sections)
Modern Optical Spectroscopy
Modern Optical Spectroscopy With Exercises and Examples from Biophysics and Biochemistry von William W Parson 1. Auflage Springer-Verlag Berlin Heidelberg 2006 Verlag C.H. Beck im Internet: www.beck.de
Raman spectroscopy: Techniques and applications in the life sciences
Review Article Advances in Optics and Photonics 1 Raman spectroscopy: Techniques and applications in the life sciences DUSTIN W. SHIPP 1,*,FARIS SINJAB 1,*, AND IOAN NOTINGHER 1,** 1 School of Physics
Optics, Light and Lasers
Dieter Meschede Optics, Light and Lasers The Practical Approach to Modern Aspects of Photonics and Laser Physics Second, Revised and Enlarged Edition BICENTENNIAL.... n 4 '':- t' 1 8 0 7 $W1LEY 2007 tri
Spectroscopic techniques: why, when, where,and how Dr. Roberto GIANGIACOMO
Spectroscopic techniques: why, when, where,and how Dr. Roberto GIANGIACOMO BASIC INFORMATION Spectroscopy uses light to analyze substances or products by describing the energy transfer between light and
36. Nonlinear optics: χ(2) processes
36. Nonlinear optics: χ() processes The wave equation with nonlinearity Second-harmonic generation: making blue light from red light approximations: SVEA, zero pump depletion phase matching quasi-phase
DEVELOPMENT OF HIGH-POWER PICOSECOND FIBER-BASED ULTRAVIOLET SOURCE
MSc in Photonics Universitat Politècnica de Catalunya (UPC) Universitat Autònoma de Barcelona (UAB) Universitat de Barcelona (UB) Institut de Ciències Fotòniques (ICFO) PHOTONICSBCN http://www.photonicsbcn.eu
Model Answer (Paper code: AR-7112) M. Sc. (Physics) IV Semester Paper I: Laser Physics and Spectroscopy
Model Answer (Paper code: AR-7112) M. Sc. (Physics) IV Semester Paper I: Laser Physics and Spectroscopy Section I Q1. Answer (i) (b) (ii) (d) (iii) (c) (iv) (c) (v) (a) (vi) (b) (vii) (b) (viii) (a) (ix)
Final exam questions ED
Final exam questions ED 2015-2016 1. Radiation a) Properties and types of radiation b) Physical parameters of radiation 2. Law of attenuation of radiation a) Experimental interpretation of the law b) Forms
OPTI 511L Fall A. Demonstrate frequency doubling of a YAG laser (1064 nm -> 532 nm).
R.J. Jones Optical Sciences OPTI 511L Fall 2017 Experiment 3: Second Harmonic Generation (SHG) (1 week lab) In this experiment we produce 0.53 µm (green) light by frequency doubling of a 1.06 µm (infrared)
NONLINEAR FREQUENCY CONVERSION IN A CRYSTALLINE WHISPERING-GALLERY MODE DISK
NONLINEAR FREQUENCY CONVERSION IN A CRYSTALLINE WHISPERING-GALLERY MODE DISK Matt T. Simons College of William & Mary Abstract We are developing high quality factor whisperinggallery mode resonator (WGMR)
Lecture cycle: Spectroscopy and Optics
Lecture cycle: Spectroscopy and Optics Thu. 13:00-15:00 / Room 1.003 15.11.2017 (Staudinger) Mischa Bonn Light-matter interaction overview I 30.11.2017 Mischa Bonn Light-matter interaction overview II
MOLECULAR SPECTROSCOPY
MOLECULAR SPECTROSCOPY First Edition Jeanne L. McHale University of Idaho PRENTICE HALL, Upper Saddle River, New Jersey 07458 CONTENTS PREFACE xiii 1 INTRODUCTION AND REVIEW 1 1.1 Historical Perspective
Supplementary Figure 1 Schematics of an optical pulse in a nonlinear medium. A Gaussian optical pulse propagates along z-axis in a nonlinear medium
Supplementary Figure 1 Schematics of an optical pulse in a nonlinear medium. A Gaussian optical pulse propagates along z-axis in a nonlinear medium with thickness L. Supplementary Figure Measurement of
ELECTRODYNAMICS OF CONTINUOUS MEDIA
ELECTRODYNAMICS OF CONTINUOUS MEDIA by L. D. LANDAU and E. M. LIFSHITZ Institute of Physical Problems, USSR Academy of Sciences Volume 8 of Course of Theoretical Physics Translated from the Russian by
THE CELL CYCLE & MITOSIS. Asexual Reproduction: Production of genetically identical offspring from a single parent.
THE CELL CYCLE & MITOSIS Asexual Reproduction: Production of genetically identical offspring from a single parent. Sexual Reproduction: The fusion of two separate parent cells that produce offspring with
PRINCIPLES OF PHYSICAL OPTICS
PRINCIPLES OF PHYSICAL OPTICS C. A. Bennett University of North Carolina At Asheville WILEY- INTERSCIENCE A JOHN WILEY & SONS, INC., PUBLICATION CONTENTS Preface 1 The Physics of Waves 1 1.1 Introduction
4. Circular Dichroism - Spectroscopy
4. Circular Dichroism - Spectroscopy The optical rotatory dispersion (ORD) and the circular dichroism (CD) are special variations of absorption spectroscopy in the UV and VIS region of the spectrum. The
1 Fundamentals of laser energy absorption
1 Fundamentals of laser energy absorption 1.1 Classical electromagnetic-theory concepts 1.1.1 Electric and magnetic properties of materials Electric and magnetic fields can exert forces directly on atoms
Light Interaction with Small Structures
Light Interaction with Small Structures Molecules Light scattering due to harmonically driven dipole oscillator Nanoparticles Insulators Rayleigh Scattering (blue sky) Semiconductors...Resonance absorption
Quadratic nonlinear interaction
Nonlinear second order χ () interactions in III-V semiconductors 1. Generalities : III-V semiconductors & nd ordre nonlinear optics. The strategies for phase-matching 3. Photonic crystals for nd ordre
A. The Cell: The Basic Unit of Life. B. Prokaryotic Cells. D. Organelles that Process Information. E. Organelles that Process Energy
The Organization of Cells A. The Cell: The Basic Unit of Life Lecture Series 4 The Organization of Cells B. Prokaryotic Cells C. Eukaryotic Cells D. Organelles that Process Information E. Organelles that
Lecturers for Week 1
Lecturers for Week 1 Prof. Cid B. de Araújo, Recife, Brazil Prof. Sergey K. Turitsyn, Birmingham, UK Dr. Miguel C. Soriano, Palma de Mallorca, Spain Prof Marcel Clerc, Santiago, Chile Prof. Yuri S. Kivshar,
Ultrashort laser applications
Ultrashort laser applications Prof. Dr. Cleber R. Mendonça Instituto de Física de São Carlos Universidade de São Paulo University of Sao Paulo - Brazil students ~ 98.000 58.000 undergrad. 30.000 grad.
Name: Class: Date: ID: A
Class: Date: Ch 7 Review Multiple Choice Identify the letter of the choice that best completes the statement or answers the question. 1. Researchers use fluorescent labels and light microscopy to a. follow
Lecture 21 Reminder/Introduction to Wave Optics
Lecture 1 Reminder/Introduction to Wave Optics Program: 1. Maxwell s Equations.. Magnetic induction and electric displacement. 3. Origins of the electric permittivity and magnetic permeability. 4. Wave
Laser Physics OXFORD UNIVERSITY PRESS SIMON HOOKER COLIN WEBB. and. Department of Physics, University of Oxford
Laser Physics SIMON HOOKER and COLIN WEBB Department of Physics, University of Oxford OXFORD UNIVERSITY PRESS Contents 1 Introduction 1.1 The laser 1.2 Electromagnetic radiation in a closed cavity 1.2.1
δf / δx = σ F (N 2 -N 1 ) ΔF~N 2 -N 1
LASER Light Amplification by Stimulated Emission of Radiation BASIC PROPERTIES O LASER RADIATION Spontaneous emission Incoherence in time Incoherence in space Polychromatic light Small energy density Non-polarized
36. Nonlinear optics: (2) processes
36. Nonlinear optics: () processes The wave equation with nonlinearity Second-harmonic generation: making blue light from red light approximations: SVEA, zero pump depletion phase matching quasi-phase
Lecture Series 5 Cell Cycle & Cell Division
Lecture Series 5 Cell Cycle & Cell Division Reading Assignments Read Chapter 18 Cell Cycle & Cell Division Read Chapter 19 pages 651-663 663 only (Benefits of Sex & Meiosis sections these are in Chapter
Cell Division. Genetic info must be copied. Each cell gets a complete copy of that info. It occurs in two main stages:
10-2 Cell Division Key Questions: 1)What is the role of chromosomes in cell division? 2) What are the main events of the cell cycle? 3) What events occur during each of the four phases of mitosis? 4) How
Fundamentals of Spectroscopy for Optical Remote Sensing. Course Outline 2009
Fundamentals of Spectroscopy for Optical Remote Sensing Course Outline 2009 Part I. Fundamentals of Quantum Mechanics Chapter 1. Concepts of Quantum and Experimental Facts 1.1. Blackbody Radiation and
Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering
Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering Lu Wei, Fanghao Hu, Yihui Shen, Zhixing Chen, Yong Yu, Chih-Chun Lin, Meng C. Wang, Wei Min * * To whom correspondence
Lecture 5: Polarization. Polarized Light in the Universe. Descriptions of Polarized Light. Polarizers. Retarders. Outline
Lecture 5: Polarization Outline 1 Polarized Light in the Universe 2 Descriptions of Polarized Light 3 Polarizers 4 Retarders Christoph U. Keller, Leiden University, keller@strw.leidenuniv.nl ATI 2016,
Chapter 3. Electromagnetic Theory, Photons. and Light. Lecture 7
Lecture 7 Chapter 3 Electromagnetic Theory, Photons. and Light Sources of light Emission of light by atoms The electromagnetic spectrum see supplementary material posted on the course website Electric
Molecular spectroscopy
Molecular spectroscopy Origin of spectral lines = absorption, emission and scattering of a photon when the energy of a molecule changes: rad( ) M M * rad( ' ) ' v' 0 0 absorption( ) emission ( ) scattering
Application of IR Raman Spectroscopy
Application of IR Raman Spectroscopy 3 IR regions Structure and Functional Group Absorption IR Reflection IR Photoacoustic IR IR Emission Micro 10-1 Mid-IR Mid-IR absorption Samples Placed in cell (salt)
Vibrational Spectroscopies. C-874 University of Delaware
Vibrational Spectroscopies C-874 University of Delaware Vibrational Spectroscopies..everything that living things do can be understood in terms of the jigglings and wigglings of atoms.. R. P. Feymann Vibrational
[ ( )] + ρ VIII. NONLINEAR OPTICS -- QUANTUM PICTURE: 45 THE INTERACTION OF RADIATION AND MATTER: QUANTUM THEORY PAGE 88
![[ ( )] + ρ VIII. NONLINEAR OPTICS -- QUANTUM PICTURE: 45 THE INTERACTION OF RADIATION AND MATTER: QUANTUM THEORY PAGE 88](/thumbs/91/107533761.jpg "[ ( )] + ρ VIII. NONLINEAR OPTICS -- QUANTUM PICTURE: 45 THE INTERACTION OF RADIATION AND MATTER: QUANTUM THEORY PAGE 88")
THE INTERACTION OF RADIATION AND MATTER: QUANTUM THEORY PAGE 88 VIII. NONLINEAR OPTICS -- QUANTUM PICTURE: 45 A QUANTUM MECHANICAL VIEW OF THE BASICS OF N ONLINEAR OPTICS 46 In what follows we draw on
DEPARTMENT OF PHYSICS UNIVERSITY OF PUNE PUNE SYLLABUS for the M.Phil. (Physics ) Course
DEPARTMENT OF PHYSICS UNIVERSITY OF PUNE PUNE - 411007 SYLLABUS for the M.Phil. (Physics ) Course Each Student will be required to do 3 courses, out of which two are common courses. The third course syllabus
OPTI510R: Photonics. Khanh Kieu College of Optical Sciences, University of Arizona Meinel building R.626
OPTI510R: Photonics Khanh Kieu College of Optical Sciences, University of Arizona kkieu@optics.arizona.edu Meinel building R.626 Important announcements Homework #2 is due Feb. 12 Mid-term exam Feb 28
A. Incorrect! The Cell Cycle contains 4 distinct phases: (1) G 1, (2) S Phase, (3) G 2 and (4) M Phase.
Molecular Cell Biology - Problem Drill 21: Cell Cycle and Cell Death Question No. 1 of 10 1. Which of the following statements about the cell cycle is correct? Question #1 (A) The Cell Cycle contains 3
Interaction X-rays - Matter
Interaction X-rays - Matter Pair production hν > M ev Photoelectric absorption hν MATTER hν Transmission X-rays hν' < hν Scattering hν Decay processes hν f Compton Thomson Fluorescence Auger electrons
Liquid Crystals IAM-CHOON 1(1100 .,4 WILEY 2007 WILEY-INTERSCIENCE A JOHN WILEY & SONS, INC., PUBLICATION. 'i; Second Edition. n z
Liquid Crystals Second Edition IAM-CHOON 1(1100.,4 z 'i; BICENTCNNIAL 1 8 0 7 WILEY 2007 DICENTENNIAL n z z r WILEY-INTERSCIENCE A JOHN WILEY & SONS, INC., PUBLICATION Contents Preface xiii Chapter 1.
Typical anisotropies introduced by geometry (not everything is spherically symmetric) temperature gradients magnetic fields electrical fields
Lecture 6: Polarimetry 1 Outline 1 Polarized Light in the Universe 2 Fundamentals of Polarized Light 3 Descriptions of Polarized Light Polarized Light in the Universe Polarization indicates anisotropy
Raman and stimulated Raman spectroscopy of chlorinated hydrocarbons
Department of Chemistry Physical Chemistry Göteborg University KEN140 Spektroskopi Raman and stimulated Raman spectroscopy of chlorinated hydrocarbons WARNING! The laser gives a pulsed very energetic and
Highenergy Nuclear Optics of Polarized Particles
Highenergy Nuclear Optics of Polarized Particles Vladimir G. Baryshevsky Research Institute for Nuclear Problems Belarusian State University 1> World Scientific NEW JERSEY LONDON SINGAPORE BEIJING SHANGHAI
A. The Cell: The Basic Unit of Life. B. Prokaryotic Cells. C. Eukaryotic Cells. D. Organelles that Process Information
The Organization of Cells A. The Cell: The Basic Unit of Life Lecture Series 4 The Organization of Cells B. Prokaryotic Cells C. Eukaryotic Cells D. Organelles that Process Information E. Organelles that
Key Concepts. n Cell Cycle. n Interphase. n Mitosis. n Cytokinesis
The Cell Cycle B-2.6: Summarize the characteristics of the cell cycle: interphase (G 1, S, G 2 ); the phases of mitosis (prophase, metaphase, anaphase, telophase); and plant and animal cytokinesis. Key
Nanophysics: Main trends
Nano-opto-electronics Nanophysics: Main trends Nanomechanics Main issues Light interaction with small structures Molecules Nanoparticles (semiconductor and metallic) Microparticles Photonic crystals Nanoplasmonics
Physics 9e/Cutnell. correlated to the. College Board AP Physics 2 Course Objectives
correlated to the College Board AP Physics 2 Course Objectives Big Idea 1: Objects and systems have properties such as mass and charge. Systems may have internal structure. Enduring Understanding 1.A:
Name Chapter 10: Chromosomes, Mitosis, and Meiosis Mrs. Laux Take home test #7 DUE: MONDAY, NOVEMBER 16, 2009 MULTIPLE CHOICE QUESTIONS
MULTIPLE CHOICE QUESTIONS 1. A bacterial chromosome consists of: A. a linear DNA molecule many times larger than the cell. B. a circular DNA molecule many times larger than the cell. C. a circular DNA
Study of Phase Transitions by Means of Raman Scattering
Study of Phase Transitions by Means of Raman Scattering M. Mahbubur Rahman Department of Physics & Physical Oceanography Memorial University, Canada Outlines Introduction Advantages of Raman spectroscopy?