Preface... (v) Acknowledgement... (vii) Introduction Chapter 1 Acids and Bases... 21
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1 CONTENTS Preface... (v) Acknowledgement... (vii) Introduction... 1 Chapter 1 Acids and Bases Experiment 1.1 To Prepare 1M HCl and Standardize it and Perform Assay of Sodium Hydrogen Carbonate Experiment 1.2 To Prepare and Standardize 0.1 M NaOH and Perform the Assay of Boric Acid Experiment 1.3 To Prepare and Standardize 0.1 M NaOH and Perform Assay of Ammonium Chloride Experiment 1.4 To Prepare and Standardize 0.5 M H 2 SO 4 and Perform Assay of Sodium Carbonate Experiment 1.5 To Determine Percentage Purity of Zinc Oxide Experiment 1.6 To Determine Percentage Purity of Sodium Phosphate Experiment 1.7 To Determine Percentage Purity of Sodium Benzoate (ix)
2 (x) Contents Experiment 1.8 Determination of Percent Purity of Sodium Potassium Tartrate Experiment 1.9 To Determine Aspirin Content in Tablet Formulation Chapter 2 Oxidation Reduction Titrations Experiment 2.1 To Prepare and Standardize 0.1N Potassium Permanganate Solution Experiment 2.2 To Prepare and Standardize 0.1M Potassium Permanganate and Perform Assay of Hydrogen Peroxide Experiment 2.3 To Determine Percentage Purity of Sodium Nitrite using Potassium Permanganate Experiment 2.4 To Prepare and Standardize 0.05N Ceric Ammonium Sulphate Experiment 2.5 To Perform Assay of Ferrous Sulphate using Ceric Ammonium Sulphate Experiment 2.6 To Perform Assay of Ferrous Fumarate using Ceric Ammonium Sulphate Experiment 2.7 To Prepare and Standardize 0.1N Iodine Solution Experiment 2.8 To Prepare and Standardize 0.1N Sodium Thiosulphate and Perform Assay of Copper Sulphate... 64
3 Contents (xi) Experiment 2.9 Determination of Ascorbic Acid using Potassium Iodate Experiment 2.10 To Prepare and Standardize 0.1N Potassium Dichromate Chapter 3 Precipitation Titration Experiment 3.1 To Prepare and Standardize Silver Nitrate Experiment 3.2 To Prepare and Standardize Ammonium Thiocyanate by Volhard s Method Experiment 3.3 To Determine Sodium Chloride by Mohr s Method Experiment 3.4 To Determine Chloride Ions by Fajan s Method Chapter 4 Gravimetric Analysis Experiment 4.1 To Study Sintered Glass Crucible Experiment 4.2 To Determine Water of Hydration in Crystalline Barium Chloride Experiment 4.3 To Determine Water of Crystallization in Copper Sulphate Experiment 4.4 To Determine Percentage of Chloride in Potassium Chloride... 90
4 (xii) Contents Chapter 5 Non-aqueous Titration Experiment 5.1 To Prepare and Standardize 0.1M Perchloric Acid Experiment 5.2 To Perform Assay of Metronidazole Benzoate Tablets I.P Experiment 5.3 Determination of Percentage Purity of Diclofenac Sodium Experiment 5.4 Determination of Percentage Purity of Methyldopa Experiment 5.5 Determination of Percentage Purity of Ephedrine Hydrochloride Experiment 5.6 Determination of Percentage Purity of Chlorpromazine Experiment 5.7 To Prepare and Standardize 0.1M Sodium Methoxide Solution Experiment 5.8 To Determine Percentage of Phenobarbitone Experiment 5.9 To Determine the Percentage Purity of Ethosuximide Chapter 6 Complexometric Titrations Experiment 6.1 To Prepare and Standardize 0.05M Disodium EDTA Experiment 6.2 To Perform Assay of Magnesium Sulphate I.P
5 Experiment 6.3 To Perform Assay of Calcium Chloride Experiment 6.4 Determine Percentage Purity of Zinc Sulphate Experiment 6.5 To Determine Percentage Purity of Calcium Gluconate Experiment 6.6 To Determine Percentage Purity of Potassium Aluminium Sulphate Experiment 6.7 To Determine Percentage Purity of Sodium Chloride Experiment 6.8 To Determine Percentage Purity of Ammonium Chloride Chapter 7 Kjeldahl Titration Experiment 7.1 To Determine Nitrogen Content in a given Sample Chapter 8 Karl-Fischer Titration Experiment 8.1 To Determine Moisture Content of Amoxicillin Trihydrate Experiment 8.2 Contents (xiii) To Determine Moisture Content of Prednisolone Sodium Phosphate
6 (xiv) Contents Chapter 9 Diazotization Titration Experiment 9.1 To Determine Sulphamethoxazole Content in Co-Trimoxazole Tablets Experiment 9.2 To Determine Dapsone Content in Tablet Formulation Chapter 10 Conductometric Titrations Experiment 10.1 To Perform Conductometric Titration of Hydrochloric Acid with Sodium Hydroxide Experiment 10.2 To Perform Conductometric Titration of Acetic acid with Sodium Hydroxide Chapter 11 Flame Photometry Experiment 11.1 Determination of Sodium and Potassium Ion Concentrations in Solution Chapter 12 Polarimetry Experiment 12.1 To Determine Sugar by Polarimetric Method Experiment 12.2 To Determine Levodopa by Polarimetric Method
7 Chapter 13 Ion Exchange Chromatography Experiment 13.1 To Determine Ion-Exchange Capacity of Resins Chapter 14 Electrophoresis Experiment 14.1 Demonstration of Separation of Proteins by SDS Gel Electrophoresis Experiment 14.2 Separation of DNA by Gel Electrophoresis Chapter 15 Thin-layer Chromatography Experiment 15.1 Identification of given Unknown Drug by using Thin Layer Chromatography Experiment 15.2 Identification of Amino Acids by using Thin Layer Chromatography Chapter 16 Column Chromatography Experiment 16.1 Contents (xv) To Separate Organic Compounds with the help of Column Chromatographic Technique
8 (xvi) Contents Experiment 16.2 To Separate Mixture of Dye with the help of Column Chromatographic Technique Chapter 17 Colorimetry Experiment 17.1 To Determine Amount of Amino Acids using Colorimetry Experiment 17.2 To Determine Amount of Blood Glucose Level using Colorimetry Chapter 18 Spectroflurometry Experiment 18.1 Determination of Riboflavin by using Spectrofluorometry Experiment 18.2 Determination of Thiamine by using Spectrofluorometry Chapter 19 UV/Visible Spectroscopy Experiment 19.1 To Verify Beer-Lambert s Law Experiment 19.2 Determination of Salicylic Acid in Synthesized Aspirin by UV-Spectrophotometric Method Experiment 19.3 Determination of Paracetamol Content in Tablet by UV-Spectrophotometric Method
9 Contents (xvii) Experiment 19.4 Determination of Amoxycillin Content in Capsule by UV-Spectrophotometric Method Experiment 19.5 Determination of Eflornithine Hydrochloride in Parenteral Formulation by UV-Spectro-photometric Method Experiment 19.6 Determination of Albendazole in Suspension Forms by UV-Spectrophotometric Method Experiment 19.7 Determination of Ranitidine in Tablet Dosage Form Spectrophotometrically Experiment 19.8 Simultaneous Estimation of Cefixime and Ofloxacin in Tablet Dosage Form Spectrophotometrically Chapter 20 Infrared Spectrophotometry Experiment 20.1 To Interpret IR Spectra of Paracetamol Experiment 20.2 To Interpret IR Spectra of Aspirin Experiment 20.3 To Interpret IR Spectra of Dexamethasone Experiment 20.4 To Interpret IR Spectra of Stigmasterol
10 (xviii) Contents Experiment 20.5 To Interpret IR Spectra of Phenoxymethyl Penicillin Potassium Chapter 21 High Performance Liquid Chromatography Experiment 21.1 Assay of Paracetamol and Aspirin in Tablets
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