APPENDIX A. Desorption Tube Packing and Conditioning
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1 APPENDIX A Desorption Tube Packing and Conditioning
2 Packing of Tenax TA Conditioning Oven Method TUBE Tenax Date Date Time Temp. Amount (g) Packed (min) ( o C) A /22/ /20/ Used in Penicillium Run B /01/ /22-11/6 D /22/ A,E,F,K,L & P E /22/ F /22/ /20/ Used in Aspergillus Run G /22/ /27-10/13 H /22/ B,D,G,I & O I /01/ K /01/ /01/ Used in Penicillium Run L /21/ B,I,K & O N /21/ O /01/ P /21/ /29/ D,E,H,L,P & Q Q /21/ (graphite seals put in oven) R /21/ T /21/ /22/ A,D,E,F,G & H Inlet /14/ (Teflon seals in oven) Inlet 5.124* 09/02/ * grams Activated Carbon
3 APPENDIX B Sampling Log (Tubes, Dates and Descriptions)
4 Samples Collected on Tenax TA for Desorption & Analysis Start Date End Date Tube Time Flow Chamber GC ID Description 01/25/ :00 01/26/ :00 25:00: Mold Run (on bread) CO2 Meter off scale 01/26/ :45 01/27/ :00 23:15: , 001 Mold Run (on bread) 02/25/1999 0:00 02/25/ :30 14:30: , g EtOH & 0.03g Isobutanol put into vessel (front and back) 02/25/ :45 02/26/ :30 24:45: , 009 Mold Run-transferred from bread to Dusty Fiberglass (front and back) 03/07/ :00 03/07/ :15 0:15: Mold on Dusty Fiberglass has been in the chamber for a week in stagnant air. 03/07/ :20 03/07/ :20 2:00: Trying to determine a sample time 03/07/ :25 03/08/ :30 24:05: Trying to determine a length of time for sampling 03/23/ :30 N/A N/A N/A N/A Fiberglass Filter #5 inoculated with Pen. C. 03/29/ :30 03/29/ :30 N/A 4:00:00 N/A 1 N/A Glass Cultivation Vessel put into 140 o C 3/29/99 for 4 hours 04/02/ :30 04/03/ :45 P 22:15: st sample set Penicillium of FF#5 (front) 04/02/ :30 04/03/ :45 N 22:15: st sample set Penicillium FF#5 (back) 04/03/ :45 04/05/1999 9:20 Q 43:35: nd sample set Penicillium FF#5 (front) 04/03/ :45 04/05/1999 9:20 T 43:35: nd sample set Penicillium FF#5 (back) 04/05/1999 9:30 04/05/ :05 R 6:35: rd sample set Penicillium FF#5 (front) 04/05/1999 9:30 04/05/ :05 L 6:35: rd sample set Penicillium FF#5 (back) 04/14/ :20 04/15/ :25 P 20:05: Blank Run (nothing in chamber) 04/15/ :00 04/16/ :30 T 20:30: Blank Run (with fiberglass filter #7) 04/18/ :15 04/19/ :30 Q 24:15: NR Blank Run duplicate (with fiberglass filter #7) 04/26/99 N/A N/A N/A High CO2 levels - FF#7 removed immersed in water and streaked onto an agar plate - negative 04/20/ :00 04/21/ :05 R 25:05: Penicillium on FF#5 (front) 04/20/ :00 04/21/ :05 L 25:05: Penicillium on FF#5 (back) 04/26/ :45 04/26/ :45 G 4:00: Penicillium on FF#5 (front) 04/26/ :45 04/26/ :45 A 4:00: Penicillium on FF#5 (back) 04/26/ :45 04/27/ :45 F 24:00: NR Blank Run (with fiberglass filter #7) (front) 04/26/ :45 04/27/ :45 E 24:00: NR Blank Run (with fiberglass filter #7) (back) 04/27/ :20 04/28/ :30 G 18:10: Penicillium on FF#5 (front) 04/27/ :20 04/28/ :30 R 18:10: Penicillium on FF#5 (middle) 04/27/ :20 04/28/ :30 F 18:10: NR Penicillium on FF#5 (back)
5 Start Date End Date Tube Time Flow Chamber GC ID Description 04/27/ :40 04/27/ :40 N/A 4:00:00 N/A 2 N/A Glass Cultivation Vessel put into 135 o C 05/02/ :20 N/A 1 N/A EXPERIMENT (enclosed reactor 1 in Aluminum Foil) 05/02/ :30 05/03/ :30 P 20:00: Blank Run (nothing in chamber) after Reactor 2 put into 135oC 4/27/99 for 4 hours 05/04/ :30 05/05/1999 8:20 Q 19:50: Blank Run (with fiberglass filter #1) 05/05/1999 8:20 05/06/1999 9:15 L 24:55: Blank Run (with fiberglass filter #1) 05/06/1999 9:20 05/07/ :15 D 27:55: Blank Run (with fiberglass filter #1) 05/09/ :20 N/A N/A N/A N/A Fiberglass Filter #1 inoculated with Pen. C. 05/09/ :20 05/10/ :15 E 24:55: Penicillium on FF#1 (front) 05/09/ :20 05/10/ :15 H 24:55: Penicillium on FF#1 (back) 05/10/ :18 05/11/ :15 T 23:57: Penicillium on FF#1 (front) 05/10/ :18 05/11/ :15 N 23:57: Penicillium on FF#1 (back) 05/11/ :18 05/12/ :25 Q 23:07: Penicillium on FF#1 (front) 05/11/ :18 05/12/ :25 D 23:07: Penicillium on FF#1 (back) 05/12/ :25 05/13/1999 6:30 G 13:05: Penicillium on FF#1 (front) 05/12/ :25 05/13/1999 6:30 P 13:05: Penicillium on FF#1 (back) 05/13/1999 6:30 05/14/ :50 R 34:20: Penicillium on FF#1 (front) 05/13/1999 6:30 05/14/ :50 L 34:20: Penicillium on FF#1 (back) 05/14/ :50 05/15/ :45 T 19:55: Penicillium on FF#1 (front) 05/14/ :50 05/15/ :45 N 19:55: Penicillium on FF#1 (back) 05/15/ :45 05/16/ : :05: Penicillium on FF#1 (front) 05/15/ :45 05/16/ : :05: Penicillium on FF#1 (back) 05/16/ :50 05/18/1999 0:25 E 29:35: Penicillium on FF#1 (front) 05/16/ :50 05/18/1999 0:25 A 29:35: Penicillium on FF#1 (back) 05/18/1999 0:25 05/18/ :30 T 21:05: Penicillium on FF#1 (front) 05/18/1999 0:25 05/18/ :30 N 21:05: Penicillium on FF#1 (back) 05/18/ :35 05/19/ :00 R 23:25: Penicillium on FF#1 (front) 05/18/ :35 05/19/ :00 L 23:25: Penicillium on FF#1 (back) 05/19/ :02 05/20/ :15 G 21:13: Penicillium on FF#1 (front) 05/19/ :02 05/20/ :15 P 21:13: Penicillium on FF#1 (back) 05/20/ :30 05/20/ :30 D 5:00: Penicillium on FF#5 (front) 05/20/ :30 05/20/ :30 Q 5:00: Penicillium on FF#5 (back) 05/20/ :15 05/27/1999 9:20 H 159:05: Penicillium on FF#1 (front) 05/20/ :15 05/27/1999 9:20 A 159:05: Penicillium on FF#1 (back) 05/24/ :47 05/24/ :47 R 1:00: Penicillium on FF#5 (front) 05/24/ :47 05/24/ :47 G 1:00: Penicillium on FF#5 (back)
6 Start Date End Date Tube Time Flow Chamber GC ID Description 05/24/ :50 05/24/ :50 L 1:00: Penicillium on FF#5 (front) 05/24/ :50 05/24/ :50 D 1:00: Penicillium on FF#5 (back) 05/27/ :12 05/27/ :32 N 0:20: Penicillium on FF#5 (front) 05/27/ :12 05/27/ :32 G 0:20: Penicillium on FF#5 (back) 05/27/ :34 05/27/ :54 P 0:20: Penicillium on FF#5 (front) 05/27/ :34 05/27/ :54 D 0:20: Penicillium on FF#5 (back) 05/27/ :00 05/27/ :25 L 0:25: Penicillium on FF#1 (front) 05/27/ :00 05/27/ :25 Q 0:25: Penicillium on FF#1 (back) 05/31/ :30 06/08/ :30 R 192:00: Penicillium on FF#1 (front) 05/31/ :30 06/08/ :30 G 192:00: Penicillium on FF#1 (back) 06/01/ :30 06/01/ :50 N/A 5:20:00 N/A 1 N/A Glass Cultivation Vessel put into 145 o C 06/03/ :00 06/04/ :00 T 19:00: Blank Run (nothing in chamber) after Reactor 1 put into 145 o C 6/1/99 for 5 hours 06/04/ :20 06/04/ :53 D 0:33: Short Blank Run (nothing in chamber) 06/04/ :45 06/04/ :15 E 0:30: Short Blank Run (fiberglass filter in chamber) 06/04/ :30 06/05/ :30 H 24:00: Blank Run (fiberglass filter in chamber) 06/08/ :00 06/08/ :30 L 0:30: Short Blank Run (fiberglass filter in chamber) 06/08/ :35 06/08/ :06 P 0:31: Short Blank Run (fiberglass filter in chamber) 06/08/ :10 N/A N/A 1 N/A Fiberglass Filter inoculated with Asp. N. 06/09/ :10 06/10/ :10 E 24:00: Aspergillus on FF#2 (front) 06/09/ :10 06/10/ :10 T 24:00: Aspergillus on FF#2 (back) 06/10/ :11 06/11/ :11 A 24:00: Aspergillus on FF#2 (front) 06/10/ :11 06/11/ :11 Q 24:00: Aspergillus on FF#2 (back) 06/11/ :13 06/12/ :17 D 24:04: Aspergillus on FF#2 (front) 06/11/ :13 06/12/ :17 N 24:04: Aspergillus on FF#2 (back) 06/12/ :45 06/13/ :15 F 24:30: Aspergillus on FF#2 (front) 06/12/ :45 06/13/ :15 P 24:30: Aspergillus on FF#2 (back) 06/13/ :20 06/14/ :55 H 24:35: Aspergillus on FF#2 (front) 06/13/ :20 06/14/ :55 L 24:35: Aspergillus on FF#2 (back) 06/14/ :00 06/15/ :00 R 24:00: Aspergillus on FF#2 (front) 06/14/ :00 06/15/ :00 D 24:00: Aspergillus on FF#2 (back) 06/15/ :03 06/16/ :35 A 24:32: Aspergillus on FF#2 (front) 06/15/ :03 06/16/ :35 T 24:32: Aspergillus on FF#2 (back) 06/16/ :39 06/17/ :35 G 23:56: Aspergillus on FF#2 (front) 06/16/ :39 06/17/ :35 P 23:56: Aspergillus on FF#2 (back) 06/17/ :37 06/19/ :40 E 50:03: Aspergillus on FF#2 (front)
7 Start Date End Date Tube Time Flow Chamber GC ID Description 06/17/ :37 06/19/ :40 N 50:03: Aspergillus on FF#2 (back) 06/19/ :50 06/22/ :46 Q 73:56: Aspergillus on FF#2 (front) 06/19/ :50 06/22/ :46 F 73:56: Aspergillus on FF#2 (back) 06/22/ :49 06/25/ :49 R 64:00: Aspergillus on FF#2 (front) 06/22/ :49 06/25/ :49 D 64:00: Aspergillus on FF#2 (back) 06/25/ :50 06/28/ :50 N 79:00: Aspergillus on FF#2 (front) 06/25/ :50 06/28/ :50 L 79:00: Aspergillus on FF#2 (back) 06/25/ :50 06/25/ :55 G 0:05: Penicillium on FF#1 06/25/ :00 07/02/ :30 P 167:30: Penicillium on FF#1 (front) 06/25/ :00 07/02/ :30 E 167:30: Penicillium on FF#1 (back) 06/28/ :50 07/01/ :55 H 72:05: Aspergillus on FF#2 (front) 06/28/ :50 07/01/ :55 Q 72:05: Aspergillus on FF#2 (back) 07/01/ :00 07/08/ :00 T 165:00: Aspergillus on FF#2 (front) 07/01/ :00 07/08/ :00 A 165:00: Aspergillus on FF#2 (back) 07/03/ :20 07/08/ :20 D 118:00: Penicillium on FF#1 (front) D&E Simultaneously collected with F&G 07/03/ :20 07/08/ :20 E 118:00: Penicillium on FF#1 (back) with a fitting from SIS 07/03/ :20 07/08/ :20 F 118:00: Penicillium on FF#1 (front) 07/03/ :20 07/08/ :20 G 118:00: Penicillium on FF#1 (back) 07/09/1999 N/A N/A N/A N/A Lab Analysis Thermal Desorption Helium Purge Flow adjusted to 100 ml/min from /13/ :00 07/20/ :30 K 168:30: Wang Penicillium on FF#1 (front) K&B Simultaneously collected with O&I 07/13/ :00 07/20/ :30 B 168:30: Penicillium on FF#1 (back) with a fitting from SIS 07/13/ :00 07/20/ :30 O 168:30: Penicillium on FF#1 (front) 07/13/ :00 07/20/ :30 I 168:30: Penicillium on FF#1 (back) 07/14/ :40 07/20/ :30 F 137:50: Wang Aspergillus on FF#2 (front) F&D Simultaneously collected with G&E 07/14/ :40 07/20/ :30 D 137:50: Aspergillus on FF#2 (back) 07/14/ :40 07/20/ :30 G 137:50: Aspergillus on FF#2 (front) 07/14/ :40 07/20/ :30 E 137:50: Aspergillus on FF#2 (back) 07/25/ :30 07/26/ :00 A 26:30: N/A 165 Ducting at 403 Hampton Ct. (Blacksburg, VA) 07/25/ :30 07/26/ :00 H 26:30:00 N/A Wang Ducting at 403 Hampton Ct. (Blacksburg, VA) 08/05/1999 9:40 08/09/ :02 T 106:22: N/A 168 Ducting at 617-B Lee St. (Blacksburg, VA) 08/05/1999 9:40 08/09/ :02 N 106:22: N/A Wang Ducting at 617-B Lee St. (Blacksburg, VA) 08/09/ :02 08/14/ :37 Q 113:35:00 N/A 173 Ducting at 617-B Lee St. (Blacksburg, VA)
8 Start Date End Date Tube Time Flow Chamber GC ID Description 08/09/ :02 08/14/ :37 R 113:35:00 N/A Wang Ducting at 617-B Lee St. (Blacksburg, VA) 09/02/ :00 09/02/ :00 N/A 3:00:00 N/A 2 N/A Glass Cultivation Vessel put into 165 o C 09/20/ :40 09/20/ :40 N/A 4:00:00 N/A 1 N/A Glass Cultivation Vessel put into 160 o C 09/20/ :00 09/27/ :00 H 168:00: Sterile FF#3 in setup (paired-dual collection) 09/20/ :00 09/27/ :00 T 168:00: NR Sterile FF#3 in setup (paired-dual collection) 09/27/ :00 09/29/ :00 B 49:00: Aspergillus on FF#3 (paired-dual collection) 09/27/ :00 09/29/ :00 Q 49:00: Aspergillus on FF#3 (paired-dual collection) 09/29/ :00 10/04/ :00 D 119:00: Wang Aspergillus on FF#3 (paired-dual collection) 09/29/ :00 10/04/ :00 O 119:00: Aspergillus on FF#3 (paired-dual collection) 10/04/ :00 10/08/ :45 G 104:45: Wang Aspergillus on FF#3 (paired-dual collection) 10/04/ :00 10/08/ :45 Q 104:45: Aspergillus on FF#3 (paired-dual collection) 10/08/ :45 10/13/ :55 B 120:10: NR Aspergillus on FF#3 (paired-dual collection) 10/08/ :45 10/13/ :55 O 120:10: Aspergillus on FF#3 (paired-dual collection) 10/17/ :00 10/21/ :48 H 98:48: Sterile FF#9 in setup (paired-dual collection) 10/17/ :00 10/21/ :48 I 98:48: Wang Sterile FF#9 in setup (paired-dual collection) 10/21/ :48 10/25/ :24 L 95:36: NR Penicillium on FF#9 (paired-dual collection) 10/21/ :48 10/25/ :24 P 95:36: Penicillium on FF#9 (paired-dual collection) 10/25/ :24 10/29/ :31 F 92:07: Wang Penicillium on FF#9 (paired-dual collection) 10/25/ :24 10/29/ :31 K 92:07: Penicillium on FF#9 (paired-dual collection) 10/29/ :31 11/02/ :15 A 96:44: Wang Penicillium on FF#9 (paired-dual collection) 10/29/ :31 11/02/ :15 E 96:44: Penicillium on FF#9 (paired-dual collection) 11/02/ :15 11/06/ :00 K 99:45: NR Penicillium on FF#9 (paired-dual collection) 11/02/ :15 11/06/ :00 P 99:45: NR Penicillium on FF#9 (paired-dual collection) Samples with information written in red do not have data from the carbon dioxide meter. Some samples were run without collecting CO 2 data. To expedite the research, two separate cultivation chambers were used, but only one CO 2 meter was available.
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10 APPENDIX C Sample Chromatograms from Penicillium on Fiberglass Filter #1 Collection period May 9 th to May 20 th
11 Sample Blank 1 and 2 May 2 nd to 3 rd and May 4 th to 5 th Sample Blank #1 - Empty Cultivation Chamber Sample Blank #2 - Fiberglass Filter #1 in Cultivation Chamber
12 Sample Blank 3 and 4 May 5 th to 6 th and May 6 th to 7 th Sample Blank #3 - Fiberglass Filter #1 in Cultivation Chamber Sample Blank #4 - Fiberglass Filter #1 in Cultivation Chamber
13 Sample Set #1 May 9 th to May 10 th Sample Set 1 - Front Tube Sample Set 1 Back Tube
14 Sample Set #2 May 10 th to May 11 th Sample Set 2 - Front Tube Sample Set 2 - Back Tube
15 Sample Set #3 May 11 th to May 12 th Sample Set 3 - Front Tube Sample Set 3 - Back Tube
16 Sample Set #4 May 12 th to May 13 th Sample Set 4 - Front Tube Sample Set 4 - Back Tube
17 Sample Set #5 May 13 th to May 14 th Sample Set 5 - Front Tube Sample Set 5 - Back Tube
18 Sample Set #6 May 14 th to May 15 th Sample Set 6 - Front Tube Sample Set 6 - Back Tube
19 Sample Set #7 May 15 th to May 16 th Sample Set 7 - Front Tube Sample Set 7 - Back Tube
20 Sample Set #8 May 16 th to May 17 th Sample Set 8 - Front Tube Sample Set 8 - Back Tube
21 Sample Set #9 May 17 th to May 18 th Sample Set 9 - Front Tube Sample Set 9 - Back Tube
22 Sample Set #10 May 18 th to May 19 th Sample Set 10 - Front Tube Sample Set 10 - Back Tube
23 Sample Set #11 May 19 th to May 20 th Sample Set 11 - Front Tube Sample Set 11 - Back Tube
24 APPENDIX D GCMS Results from the Chemistry Department (Set #1)
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