Practical Faster GC Applications with High-Efficiency GC Columns and Method Translation Software
|
|
- Albert Malone
- 5 years ago
- Views:
Transcription
1 Practical Faster GC Applications with High-Efficiency GC Columns and Method Translation Software GC Columns and Consumables Mark Sinnott Application Engineer January 8 th, 2008 Page 1
2 Questions to Ask What information do you need from your analysis? Do you have more baseline than you need between your peaks? Do you need to resolve all of the components? Page 2
3 Variables for Shortening Run Times Stationary Phase Temperature Programming Carrier Gas: type and linear velocity Shorten Column Length Decrease Film Thickness Decrease Internal Diameter Page 3
4 Resolution N k α 1 R = s 4 k + 1 α Efficiency Retention Selectivity N = ƒ (gas, L, r c ) k = ƒ (T, d f, r c ) α = ƒ (T, phase) L = Length r c = column radius d f = film thickness T = temperature Page 4
5 Resolution N k α 1 R = s 4 k + 1 α Efficiency Retention Selectivity N = ƒ (gas, L, r c ) k = ƒ (T, d f, r c ) α = ƒ (T, phase) L = Length r c = column radius d f = film thickness T = temperature Page 5
6 Stationary Phase - Common Types Siloxane polymers Poly(ethylene) glycols Porous polymers Page 6
7 Selectivity Relative spacing of the chromatographic peaks The result of all non-polar, polarizable and polar interactions that cause a stationary phase to be more or less retentive to one analyte than another Page 7
8 Optimizing Selectivity Match analyte polarity to stationary phase polarity - like dissolves like(oil and water don t mix) Take advantage of unique interactions between analyte and stationary phase functional groups Page 8
9 Start with the Right Phase DB-1 15m x 0.32mm, 0.25µm Oven: 40 C for 2 min C at 5 C/min Time (min.) DB-Wax 15m, 0.32mm, 0.25µm Oven: C at 20 C/min Time (min.) Page 9
10 Resolution N k α 1 R = s 4 k + 1 α Efficiency Retention Selectivity N = ƒ (gas, L, r c ) k = ƒ (T, d f,r c ) α = ƒ (T, phase) L = Length r c = column radius d f = film thickness T = temperature Page 10
11 Column Length and Efficiency (Theoretical Plates) Length (m) n 15 69, , , mm ID n/m = 4630 (for k = 5) Page 11
12 Column Length and Resolution R α n α L Length X 4 = Resolution X 2 t α L Page 12
13 Column Length VS Resolution and Retention: Isothermal R= min R= min R= min 15 m 30 m 60 m Double the plates, double the time but not double the the resolution Page 13
14 DECREASE THE LENGTH DB-5 30 m 0.53 mm I.D., 0.5 µm DB-5 15 m 0.53 mm I.D., 0.5 µm 1. Benzene 2. Toluene 3. Ethylbenzene 4. m,p-xylene 5. o-xylene BTEX Carrier: Helium, 36 cm/sec at 40 c Oven : 40 C for 3 min, 5 /min to 100 C Page 14
15 Column Length and Cost 15m 30m 60m $ $ $ $ $ $ $ Page 15
16 Length Summary If you Decrease Length: Efficiency Resolution Analysis Time Pressure Cost Decrease Decrease Decrease Decrease Decrease Page 16
17 Resolution N k α 1 R = s 4 k + 1 α Efficiency Retention Selectivity N = ƒ (gas, L, r c ) k = ƒ (T, d f, r c ) α = ƒ (T, phase) L = Length r c = column radius d f = film thickness T = temperature Page 17
18 Column Diameter - Theoretical Efficiency Total Plates I.D. (mm) n/m 5 m N ~ 112, , m N ~ 112, , , m N ~ 112, m N ~ 112, k = Page 18
19 Different Column I. D. Equal Phase Ratios Column: DB m, 0.53 mm, 3 m Carrier: Oven: Helium, 40(cm/sec) 65 C Injection: Split Detector: FID Column: DB m, 0.32 mm, 1.8 m Time (min) Page 19
20 PHASE RATIO (β) Film Thickness Column Dimensions Phase Ratio β 30 m x.53 mm x 3.0 μm m x.32 mm x 1.8 μm 44 K C = k β β = r 2d f Page 20
21 Column Diameter and Capacity I.D. (mm) Capacity (ng) Like Polarity Phase/Solute 0.25 µm film thickness Page 21
22 Column Diameter - Inlet Head Pressures (Helium) I.D (mm) Pressure (psig) meters Hydrogen pressures x 1/ Page 22
23 Column Diameter and Carrier Gas Flow Lower flow rates: Smaller diameter columns Higher flow rates: Larger diameter columns Low flow rates : GC/MS High flow rates: Headspace, purge & trap Page 23
24 Diameter Summary If you decrease the inside diameter: Efficiency Resolution Pressure Capacity Flow rate Increase Increase Increase Decrease Decrease Page 24
25 Combining a Change in Length With a Change in Diameter Decrease Length Decrease Diameter Efficiency Analysis Time Page 25
26 Carrier Gas Considerations Best velocity? Optimal range of velocities Too low or high results in loss of resolution Balance resolution and analysis time BUT... there is no LAW against going FASTER Page 26
27 Carrier Gas Considerations Van Deemter Curve 1.00 Excessive Diffusion h He Poor Mass Transfer 0.25 ū opt H 2 OPGV u (cm/sec) Page 27
28 Carrier Gas Considerations Utilizing Computer Controlled Flow Ramping (EPC) Lonely Late Eluters? = P Decrease retention and overall run time by increasing pressure (speed up the gas!) Page 28
29 Easy Options with Method Translation Software Different Column Dimensions Switch He to H 2 Carrier Gas and Try Faster Velocities Same Column & Gas Type but Faster Velocities Combination of all of the above Page 29
30 CLP-Pesticides - Original Improved Method 0.32mm I.D., Helium Carrier Gas , Column: DB-XLB 30m x 0.32mm i.d., 0.25µm Carrier: He, constant flow, 38 cm/s at 120 C Injector: Pulsed Splittless, 220 C Pulse pressure & time: 35psi for 1.15min Oven: µL, 50ppb 120 C for 1.17min 120 C to 160 C at 25 /min 160 C to 260 C at 10 /min 260 C to 300 C (4min) at 15 /min Detector: µ-ecd, 320 C Ar/CH4 (P5) makeup gas at 60mL/min <16 minutes Page 30
31 Input Original Method Parameters Page 31
32 Same Column and Gas Type Fast Analysis (OPGV) Transfer same dimensions New Velocity New Temp. Program Page 32
33 Same Column and Gas Type Fast Analysis (OPGV) Before No Real Changes in Resolution! Only Time! <14 minutes Page 33
34 Same Column and Gas type None mode, Try higher velocities New calculated temp. program Page 34
35 Same Column and Gas type None mode, Higher velocities Before Co-elution (poorer) cm/sec Co-elution (poorer) <8 minutes 65 cm/sec <10 minutes Close elution (similar) 50 cm/sec Page 35
36 Same Column, Hydrogen Carrier Gas Translate Only Page 36
37 Same Column, H 2 Carrier Gas Translate Only Before No Real Changes in Resolution % faster! 11 minutes Page 37
38 Same Column, H2 Carrier Gas, Higher Velocities Page 38
39 Same Column, H2 Carrier Gas, Even Higher Velocities Co-elution Before cm/sec Co-elution 7 minutes 80 cm/sec Co-elution <8 minutes 70 cm/sec Page 39
40 New Column Dimensions, H 2 Gas, Translate Only Input NEW dimensions Close enough (got lucky) New Velocity New Temp. Program Page 40
41 New Column Dimensions, H2 Gas, Translate Only Page 41
42 New Column Dimensions, H2 Gas, Translate Only Page 42
43 New Column Dimensions, H2 Gas, Fast Analysis New Velocity New Temp. Program Page 43
44 New Column Dimensions, H2 Gas, Fast Analysis Better Resolution and Faster Analysis! Page 44
45 New Column Dimensions, H2 Gas, Higher Velocities Page 45
46 New Column Dimensions, H2 Gas, Higher Velocities 105 cm/sec? 5.5 minutes! 95 cm/sec <6 minutes 85 cm/sec Page 46
47 Final Method Used at EPA Column: DB-XLB 20m x 0.18mm i.d., 0.18µm Carrier: H2, constant flow, 77.3cm/s at 120 C Injector: Pulsed Splittless, 220 C Pulse pressure & time: 35psi for 0.5min Flow ramp at 6.25min of 99mL/min 2 to 3mL/min 2mm i.d. liner Oven: µL, 50ppb 120 C for 0.49min 120 C to 160 C at 59.4 /min 160 C to 260 C at 23.7 /min 260 C to 300 C (1.69min) at 35.6 /min Detector: µ-ecd, 320 C Ar/CH4 (P5) makeup gas at 60mL/min Page 47
48 Final Method Used at EPA DB-XLB DB-17ms Page 48
49 Food/Fragrance Method translation Page 49
50 Food/Fragrance Method translation Page 50
51 Spearmint Oil 10.6 min Δ min (0.18 mm, H 2 Carrier) 17.7 min Δ -9.7 min (0.18 mm, He Carrier) (0.25 mm, He Carrier) 27.4 min Time (min) Page 51
52 Spearmint Oil Resolution Check Δ min (0.18 mm, H 2 Carrier) 10.6 min Δ -9.7 min (0.18 mm, He carrier) 17.7 min (0.25 mm, He Carrier) 27.4 min Page 52
53 Resolution Maintained Compound s Compound Resolution 0.25 mm 0.18 mm 0.18 mm Helium Helium Hydrogen Sabinene β-pinene α-terpinene p-cymene Speed Gain N/A 35% 61% Page 53
54 CONCLUSIONS Stationary Phases Chosen for optimized selectivity Diameter Smaller allows shorter length but has less capacity Make Small Changes Again think capacity Carrier Gas Hydrogen, high velocity, but can still go fast with He Temperature Program Scale properly to preserve elution pattern Method Translation Software FREE, reliable Flow Ramp Increase at end of run for late eluters (if necessary) Page 54
55 Thank you! TECHNICAL SUPPORT Agilent #4, #1 Page 55
56 Thank you for attending Agilent Technologies e-seminar today. For a full listing of our e-seminar offerings as well as our hands-on training classes, please go to our website at: Or register for Stay current with e-notes to receive regular updates Page 56 Slide 56
57 Upcoming e-seminars NEW! Carrier Gases in Capillary GC - Series 1 January 15, :00 p.m. EST Introduction to Capillary GC - Series 2 February 20, :00 p.m. EST Selection of a Capillary GC Column - Series 3 March 13, :00 p.m. EST Page 57 Sli de
58 Looking for more information on Agilent s GC Systems and Software? Agilent offers a full range of GC training courses including hands-on courses with the latest 7890 GC equipment as well as 6890 and 5890 courses. Each course includes a course manual for future reference and a certificate of completion. All courses are taught by industry experts. Call , Option 5 or visit to register today! Page 58
59 New On Demand Webinar: Utilizing Sub-Two Micron Particles to Optimize HPLC Methods A Four Part Workshop on Managing Chemistry and Pressure for Faster and More Efficient HPLC Separations Be sure to register after today s s event. Our measure is your success. Page 59
Practical Faster GC Applications with High-Efficiency GC Columns and Method Translation Software
Practical Faster GC Applications with High-Efficiency GC Columns and Method Translation Software High Efficiency GC Columns Page 1 Variables for Shortening Run Times Stationary Phase Shorten Column Length
More informationSelection of a Capillary GC Column
Selection of a Capillary GC Column Mark Sinnott Application Engineer March 13, 2008 Page 1 Typical Gas Chromatographic System Mol-Sieve Traps Fixed Restrictors Regulators Injection Port Detector Electrometer
More informationSecrets of GC Column Dimensions
Secrets of GC Column Dimensions GC Columns and Consumables Simon Jones Application Engineer May 20, 2008 Slide 1 Secrets of GC Column Dimensions Do I have the right column phase? Resolution Equation Changes
More informationColumn Dimensions. GC Columns and Consumables. Mark Sinnott Application Engineer. March 12, 2010
Secrets of GC Column Dimensions GC Columns and Consumables Mark Sinnott Application Engineer Folsom California March 12, 2010 Page 1 Secrets of GC Column Dimensions Do I have the right column phase? Resolution
More informationIntroduction to Capillary GC
Introduction to Capillary GC LC Columns and Consumables Simon Jones Chromatography Applications Engineer February 20, 2008 Page 1 Introduction to Capillary GC t r K c?? Kβ k = - tr t m? t m R s Page 2
More informationSelection of a Capillary
Selection of a Capillary GC Column - Series 3 Mark Sinnott Application Engineer March 19, 2009 Page 1 Typical Gas Chromatographic System Mol-Sieve Traps Fixed Restrictors Regulators Injection Port Detector
More informationIntroduction to Capillary GC. Page 1. Agilent Restricted February 2, 2011
?? Kβ? Page 1 Typical GC System Gas supply Injector Detector Data handling GAS Column Oven Page 2 CARRIER GAS Carries the solutes down the column Selection and velocity influences efficiency and retention
More informationRapid Analysis of Food and Fragrances Using High-Efficiency Capillary GC Columns. Application. Authors. Abstract. Introduction
Rapid Analysis of Food and Fragrances Using High-Efficiency Capillary GC Columns Application Food, Flavors and Fragrances Authors Mark Sinnott and Simon Jones Agilent Technologies, Inc. 9 Blue Raving Road
More informationIntroduction to Capillary GC
?? Kβ? Page 1 Typical GC System Gas supply Injector Detector Data handling GAS Column Oven Page 2 CARRIER GAS Carries the solutes down the column Selection and velocity influences efficiency and retention
More informationPractical Fast Gas Chromatography for Contract Laboratory Program Pesticide Analyses *
Practical Fast Gas Chromatography for Contract Laboratory Program Pesticide Analyses * L. Wool and D. Decker EPA, 10625 Fallstone Road, Houston, TX 77099 Abstract An approach to shortening the analysis
More informationUnderstanding the Capillary GC Column: How to Choose the Correct Type and Dimension
Understanding the Capillary GC Column: How to Choose the Correct Type and Dimension Simon Jones Application Engineer Things to Consider Is it Volatile enough to chromatograph by GC? Is it a Gas or a Liquid?
More informationGC Resolution Do You See What I See?
GC Resolution Do You See What I See? Column Dimensions and Carrier Gas Optimization Deans Switch Page 1 Variables for Maximizing Resolution Optimized Stationary Phase Longer Column Length Decrease Internal
More informationChoosing the Correct GC Column Dimensions and Stationary Phase
Choosing the Correct GC Column Dimensions and Stationary Phase Daron Decker Chromatography Technical Specialist Page 1 Nothing is useless it can always serve as a bad example Custom Column: 150 m x 250
More informationUnderstanding Gas Chromatography
Understanding Gas Chromatography What is Really Going on Inside the Box? Simon Jones GC Applications Engineer Page 1 Group/Presentation Title Month ##, 200X ?? K? Page 2 Typical GC System Gas supply Injector
More informationHow To Select the Correct GC Column. Simon Jones Application Engineer
How To Select the Correct GC Column Simon Jones Application Engineer Things to Consider Is it Volatile enough to chromatograph by GC? Is it a Gas or a Liquid? How are we getting the Sample Injected? What
More informationAuthor. Abstract. Introduction
Improved Performance for the Analysis of Aromatics in Gasoline by ASTM Method D5769 Using the Agilent 5973 inert Gas Chromatography/Mass Spectrometry System Application Author James D. McCurry Agilent
More informationA New Web-Based Application for Modeling Gas Chromatographic Separations. Dr. Hansjoerg Majer Restek Corporation Market Development Manager Europe
A New Web-Based Application for Modeling Gas Chromatographic Separations Dr. Hansjoerg Majer Restek Corporation Market Development Manager Europe The Method Development Process in GC $ SUCCESS! Developing
More informationCapillary GC Column Selection and Method Development A Primer on Column Parameters and Instrument Conditions
Capillary GC Column Selection and Method Development A Primer on Column Parameters and Instrument Conditions Michael D. Buchanan September 11, 2014 sigma-aldrich.com/analytical 2012 Sigma-Aldrich Co. All
More informationAnalysis of Trace (mg/kg) Thiophene in Benzene Using Two-Dimensional Gas Chromatography and Flame Ionization Detection Application
Analysis of Trace (mg/kg) Thiophene in Using Two-Dimensional Gas Chromatography and Flame Ionization Detection Application Petrochemical Authors James D. McCurry and Bruce D. Quimby Agilent Technologies
More informationA New Web-Based Application for Modeling Gas Chromatographic Separations
A New Web-Based Application for Modeling Gas Chromatographic Separations Jaap de Zeeuw**, Rebecca Stevens*, Amanda Rigdon, Linx Waclaski*and Dan Li* *Restek Corporation, Bellefonte, PA, USA **Restek Corporation,
More informationIntroduction to Gas Chromatography
Introduction to Gas Chromatography 31-1 Objectives To know what is chromatography To understand the mechanism of compound separation To know the basic of gas chromatography system 31-2 Chromatography Definition
More informationIntroduction and Principles of Gas Chromatography
Introduction and Principles of Gas Chromatography Jaap de Zeeuw Restek, Middelburg, The Netherlands Jaap.dezeeuw@restek.com Definition and Uses of Gas Chromatography GC Components and Types of Columns
More informationHydrogen as a Carrier Gas in the Analysis of Polar/Non-Polar Compounds Using the Polyarc System. Application Note. Author. Introduction.
Hydrogen as a Carrier Gas in the Analysis of Polar/Non-Polar Compounds Using the Polyarc System Application Note Chemicals Author Bob Moyer Sr. Research Scientist, R&D Sasol USA, Performance Chemicals
More informationThe Importance of Area and Retention Time Precision in Gas Chromatography Technical Note
The Importance of Area and Retention Time Precision in Gas Chromatography Technical Note Abstract Area and retention time are the two primary measurements in gas chromatography. The precision with which
More informationSpeakers. Moderator. John V Hinshaw GC Dept. Dean CHROMacademy. Tony Taylor Technical Director CHROMacademy. Dave Walsh Editor In Chief LCGC Magazine
Webcast Notes Type your questions in the Submit Question box, located below the slide window You can enlarge the slide window at any time by clicking on the Enlarge Slides button, located below the presentation
More informationWater Injections in GC - Does Water Cause Bleed?
Water Injections in GC - Does Water Cause Bleed? Eberhardt Kuhn Applications Chemist April 4, 2001 Practical Advice and Useful Tips for the Analysis of Semivolatile Organics by GC and GC/MS 11:00 a.m.
More informationFast Analysis of Aromatic Solvent with 0.18 mm ID GC column. Application. Authors. Introduction. Abstract. Gas Chromatography
Fast Analysis of Aromatic Solvent with.8 mm ID GC column Application Gas Chromatography Authors Yun Zou Agilent Technologies (Shanghai) Co. Ltd. Ying Lun Road Waigaoqiao Free Trade Zone Shanghai 3 P.R.
More informationTheory and Instrumentation of GC. Chromatographic Parameters
Theory and Instrumentation of GC Chromatographic Parameters i Wherever you see this symbol, it is important to access the on-line course as there is interactive material that cannot be fully shown in this
More informationUSP <467> Headspace Residual Solvent Assay with a HT3 Headspace Instrument
Application Note Abstract The US Pharmacopeia recently released USP as the current monograph for determining residual solvents in pharmaceutical products by static headspace. The USP classified these
More informationNew ZB-5HT Inferno The World s Highest Temperature Non-Metal GC Column
New ZB-5HT Inferno The World s Highest Temperature Non-Metal GC Column The World's First Non-metal 5% Phenyl Phase GC Column Rated to 430 C * Specially processed for thermal stability up to 430 C A true
More informationAnalysis of USP Method <467> Residual Solvents on the Agilent 8890 GC System
Application Note Residual Solvent Analysis of USP Method Residual Solvents on the Agilent 889 GC System Author Lukas Wieder, Jie Pan, and Rebecca Veeneman Agilent Technologies, Inc. 8 Centerville Road
More informationStudy of Residual Solvents in Various Matrices by Static Headspace
Application Note Abstract United States Pharmacopeia (USP) chapter is a widely used method for identifying and quantifying Organic Volatile Impurities (OVI) used in the production of pharmaceuticals.
More informationSupelco Ionic Liquid GC Columns Introduction to the Technology
Supelco Ionic Liquid GC Columns Introduction to the Technology Updated: -Jan-203 Agenda Overview GC Column Polarity Scale Temperature Effects on Selectivity Column Selectivity: QC Test Mix (0.2 mm I.D.
More informationSimultaneous Compound Identification and Quantification with Parallel Polyarc /FID and MS
Simultaneous Compound Identification and Quantification with Parallel Polyarc /FID and MS Application Note Multi-detector Splitter Authors Charlie Spanjers and Andrew Jones Activated Research Company 7561
More informationQuantification of Pesticides in Food without Calibration using GC/FID with the Polyarc Reactor
Quantification of Pesticides in Food without Calibration using GC/FID with the Polyarc Reactor Application Note Pesticides Authors Charlie Spanjers and Paul Dauenhauer University of Minnesota, Twin Cities
More informationOptimizing GC Parameters for Faster Separations with Conventional Instrumentation
Optimizing GC Parameters for Faster Separations with Conventional Instrumentation Anila I. Khan, Thermo Fisher Scientific, Runcorn, Cheshire, UK Technical Note 243 Key Words TraceGOLD fast GC analysis
More informationGet Selective. By Jaap de Zeeuw
34 Get Selective Modern narrow bore columns have made chromatographers lazy when it comes to stationary phase selection. Here s how getting back to basics in gas chromatography by using selectivity can
More informationRoger Bardsley, Applications Chemist; Teledyne Tekmar Page 1
Application Note Meeting the Korean Method ES 04602.1b for Vinyl Chloride, Acrylonitrile, and Bromoform ( 염화비닐, 아크릴로니트릴, 브로모포름 ) with Static and Dynamic Headspace GC/MS Roger Bardsley, Applications Chemist;
More informationGas Chromatography (Chapter 2 and 3 in The essence of chromatography)
Gas Chromatography 1. Introduction. Stationary phases 3. Retention in Gas-Liquid Chromatography 4. Capillary gas-chromatography 5. Sample preparation and injection 6. Detectors (Chapter and 3 in The essence
More informationAn Advanced Base Deactivated Capillary Column for analysis of Volatile amines Ammonia and Alcohols.
An Advanced Base Deactivated Capillary Column for analysis of Volatile amines Ammonia and Alcohols. Jaap de Zeeuw, Ron Stricek and Gary Stidsen Restek Corp Bellefonte, USA To analyze basic compounds at
More informationTrajan SGE GC Columns
Trajan Scientific and Medical Trajan SGE GC Columns Trajan Scientific and Medical Our focus is on developing and commercializing technologies that enable analytical systems to be more selective, sensitive
More informationA New PEG GC Column with Improved Inertness Reliability and Column Lifetime Agilent J&W DB-WAX Ultra Inert Polyethylene Glycol Column
A New PEG GC Column with Improved Inertness Reliability and Column Lifetime Agilent J&W DB-WAX Ultra Inert Polyethylene Glycol Column Competitive Comparison Authors Ngoc-A Dang and Allen K. Vickers Agilent
More informationColumn Selection. there is more to life than a boiling point column. Jaap de Zeeuw Restek Corporation, The Netherlands. Copyrights: Restek Corporation
Column Selection there is more to life than a boiling point column Jaap de Zeeuw Restek Corporation, The Netherlands Stationary Phase Selectivity Boiling Point versus Solubility Retention of a compound
More informationGAS CHROMATOGRAPHY (GC)
GAS CHROMATOGRAPHY (GC) Pre-Lab Questions Questions are to be answered before the beginning of the laboratory. The answers are due at the beginning of each experiment (the questions are for credit and
More informationCHEM340 Tutorial 4: Chromatography
CHEM340 Tutorial 4: Chromatography 1. The data in the table below was obtained from a chromatogram obtained with a 10 cm liquid chromatography column. Under the conditions used, the compound uracil is
More informationResidual Solvents in Pharmaceuticals by USP Chapter <467> Methodology
APPLICATION NOTE Gas Chromatography Author: David Scott PerkinElmer, Inc. Shelton, CT Residual Solvents in Pharmaceuticals by USP Chapter Methodology Introduction The synthesis of active pharmaceutical
More informationProduct Brief. - Hydrocarbons alkanes, alkenes, alkynes, dienes including natural gas, refinery gas, liquified petroleum gas
Agilent Porous Polymer PLOT Columns: New Products, Expanded Uses, Prices Cut in Half! Product Brief Need improved resolution of small volatile compounds? Didn't try a PLOT column due to high price, short
More informationActivity in the FID Detection Port: A Big Problem if Underestimated
Activity in the FID Detection Port: A Big Problem if Underestimated Jaap de Zeeuw, Restek Corporation, Middelburg, The Netherlands It is commonly known in gas chromatography, that many problems can be
More informationSo Many Columns! How Do I Choose? Daron Decker Chromatography Technical Specialist
So Many Columns! How Do I Choose? Daron Decker Chromatography Technical Specialist GC Columns Wall Coated Open Tubulars Liquid phase coated capillaries Internal Diameter 0.05 0.53mm Length 5m 100m Porous
More informationFast USEPA 8270 Semivolatiles Analysis Using the 6890/5973 inert GC/MSD with Performance Electronics Application
Fast USEPA 8270 Semivolatiles Analysis Using the 6890/5973 inert GC/MSD with Performance Electronics Application Environmental Analysis Author Mike Szelewski Agilent Technologies, Inc. 2850 Centerville
More informationGC Analysis of Polybrominated Flame Retardants Application
GC Analysis of Polybrominated Flame Retardants Application Environmental Authors Eberhardt Kuhn, Jason Ellis Agilent Technologies, Inc. 91 Blue Ravine Rd, Folsom, CA 9563 USA Steve Wilbur Agilent Technologies,
More informationANNE JUREK. Abstract: soils and are found. in can also with GC/MS. poster. in series. option for. There are problems. analytes. in methanol.
Determination of Volatile Petroleum Hydrocarbons ANNE JUREK Abstract: Due to current events, the importance of determining volatile petroleum hydrocarbons in both soils and waters has become an issue.
More informationEvaluation of a New Analytical Trap for Gasoline Range Organics Analysis
Abstract Purge and Trap (P&T) is a concentration technique used for the analysis of Volatile Organic Compounds (VOCs). The major component of any P&T system is the analytical trap. This trap is responsible
More informationApplication Note. Abstract. Introduction. Experimental-Instrument Conditions. By: Anne Jurek
Automated Handling Techniques for the Analysis of Elevated Volatile Organic Compound (VOC) Concentrations in Soils Utilizing the Atomx Concentrator/Multimatrix Autosampler. Application Note By: Anne Jurek
More informationResidual solvents analysis using an Agilent Intuvo 9000 GC system
Residual solvents analysis using an Intuvo GC system Technology advantage: simplified dual column analysis with Flow Chip modularity Introduction USP defines a method for analysis of residual solvents
More informationChemistry Instrumental Analysis Lecture 27. Chem 4631
Chemistry 4631 Instrumental Analysis Lecture 27 Gas Chromatography Introduction GC covers all chromatographic methods in which the mobile phase is gas. It may involve either a solid stationary phase (GSC)
More informationVolatile organic compounds (VOCs):
Volatile organic compounds (VOCs): Organic chemicals with a high vapour pressure at room temperature. High vapour pressure results from a low boiling point. The World Health Organization (WHO) defined
More informationChromatography. Gas Chromatography
Chromatography Chromatography is essentially the separation of a mixture into its component parts for qualitative and quantitative analysis. The basis of separation is the partitioning of the analyte mixture
More informationThe Focus Robotic Sample Processor as a Tool for the Multiple Analysis of Samples using Complementary Techniques
Application Note No. 085 The Focus Robotic Sample Processor as a Tool for the Multiple Analysis of Samples using Complementary Techniques Diane Nicholas Multiple techniques using one instrument Simple
More informationSTANDARD OPERATING PROCEDURES
PAGE: 1 of 12 CONTENTS 1.0 SCOPE AND APPLICATION 2.0 METHOD SUMMARY 3.0 SAMPLE PRESERVATION, CONTAINERS, HANDLING, AND STORAGE 4.0 INTERFERENCES AND POTENTIAL PROBLEMS 5.0 EQUIPMENT/APPARATUS 6.0 REAGENTS
More informationAccurate Analysis of Fuel Ethers and Oxygenates in a Single Injection without Calibration Standards using GC- Polyarc/FID. Application Note.
Accurate Analysis of Fuel Ethers and Oxygenates in a Single Injection without Calibration Standards using GC- Polyarc/FID Application Note Volatile Organic Compounds (VOCs) Author Andrew Jones Activated
More informationChapter 31 Gas Chromatography. Carrier Gas System
Chapter 31 Gas Chromatography GAS-LIQUID CHROMATOGRAPHY In gas chromatography, the components of a vaporized sample are fractionated as a consequence of being partitioned between a mobile gaseous phase
More informationDetermination of Total Volatile Organic Compounds in Indoor Air Using Agilent 7667A mini TD and 7820A GC
Determination of Total Volatile Organic Compounds in Indoor Air Using Agilent 77A mini TD and 70A GC Application Note Environmental Authors Tingting Bu, Xiaohua Li Agilent Technologies (Shanghai) Co.,
More informationGUIDELINES FOR THE DESIGN OF CHROMATOGRAPHIC ANALYTICAL METHODS INTENDED FOR CIPAC COLLABORATIVE STUDY
Page 1 of 13 CIPAC/4105/R GUIDELINES FOR THE DESIGN OF CHROMATOGRAPHIC ANALYTICAL METHODS INTENDED FOR CIPAC COLLABORATIVE STUDY Prepared for CIPAC by Dr M J Tandy*, P M Clarke and B White (UK) The rapid
More informationTrace analysis of mesityl oxide and diacetone alcohol in pharmaceuticals by capillary gas chromatography with flame ionization detection
Trade Science Inc. September 2009 Volume 8 Issue 3 ACAIJ, 8(3) 2009 [346-349] Trace analysis of mesityl oxide and diacetone alcohol in pharmaceuticals by capillary gas chromatography with flame ionization
More information-xt. -xt SYSTEM. Specifications for PAL-xt Systems. Valid for PAL-xt System models only. Prep and Load Platform
-xt SYSTEM Prep and Load Platform -xt Specifications for PAL-xt Systems Valid for PAL-xt System models only Revised March 2013 V5 PAL is a registered trademark of CTC Analytics AG Switzerland -xt SYSTEM
More informationAnalysis of BTEX in Natural Water with SPME
Analysis of BTEX in Natural Water with SPME Application Note Environmental Author Giordano Vassalli Sezione Protezione Aria Introduction Benzene, toluene, ethylbenzene and xylene (BTEX) isomers are monocyclic
More informationGas Chromatography. Presented By Mr. Venkateswarlu Mpharm KTPC
Gas Chromatography Gas Chromatography Presented By Mr. Venkateswarlu Mpharm KTPC What is Gas Chromatography? It is also known as Gas-Liquid Chromatography (GLC) GAS CHROMATOGRAPHY Separation of gaseous
More informationThe Effects of Carrier Gas Viscosity and Diffusion on Column Efficiency in Capillary Gas Chromatography
Page 1 of 5 Return The Effects of Carrier Gas Viscosity and Diffusion on Column Efficiency in Capillary Gas Chromatography Stephanye D. Armstrong and Harold M. McNair Department of Chemistry, Virginia
More informationChlorinated Compounds in Hydrocarbon Streams Using a Halogen Specific Detector (XSD)
Introduction There has been an increase in concern for the detection and removal of organic chloride species from crude aromatic, naphtha, and other hydrocarbon streams. One process called catalytic reforming
More informationUSEPA Methods 8270 and 8260 on a Single GCMS Without Changing Columns
USEPA Methods 8270 and 8260 on a Single GCMS Without Changing Columns Richard Whitney, Laura Chambers, Clifford Taylor Shimadzu Scientific Instruments, Inc. Columbia, MD Spotlight on Method 8270 Instrumentation
More informationHeadspace Technology for GC and GC/MS: Features, benefits & applications
Headspace Technology for GC and GC/MS: Features, benefits & applications Karima Baudin Oct 2015 Why use Headspace? Very Simple no to minimum sample prep Robust enhance uptime Non-detectable carry-over
More informationCHAPTER 6 GAS CHROMATOGRAPHY
CHAPTER 6 GAS CHROMATOGRAPHY Expected Outcomes Explain the principles of gas chromatography Able to state the function of each components of GC instrumentation Able to state the applications of GC 6.1
More informationThe Analysis of Residual Solvents in Pharmaceutical Products Using GC-VUV and Static Headspace
The Analysis of Residual Solvents in Pharmaceutical Products Using GC-VUV and Static Headspace The Analysis of Residual Solvents in Pharmaceutical Products Using GC-VUV and Static Headspace_Rev3 Introducing
More informationAnalysis of Geosmin and 2-Methylisoborneol Utilizing the Stratum PTC and Aquatek 70
Analysis of Geosmin and 2-Methylisoborneol Utilizing the Stratum PTC and Aquatek 70 Application Note Abstract Geosmin and 2-Methylisoborneol are organic compounds that have a distinct scent. These compounds
More informationDetermination of VOC in Artificial Runway by Multiple Headspace Extraction-GC/MS. Gas Chromatography/ Mass Spectrometry APPLICATION.
APPLICATION NOTE Gas Chromatography/ Mass Spectrometry Author: Kira. Yang PerkinElmer, Inc. Shanghai, China Determination of VOC in Artificial Runway by Multiple Headspace Extraction-GC/MS Introduction
More informationChem 230, Fall, 2014 Homework Set # 3 Short Answer SOLUTIONS
Chem 230, Fall, 2014 Homework Set # 3 Short Answer SOLUTIONS 1. List two advantages of temperature programming in GC. a) Allows separation of solutes with widely varying retention factors in a reasonable
More informationGC-CI-MS analysis of TMS derivatives
GC-CI-MS analysis of TMS derivatives This method describes analysis of TMS derivatives with methane chemical ionisation (CI) rather than the more normal electron impact (EI) ionisation. Methane CI is a
More informationDetermination of Volatile Substances Proof of Food Adulteration
ANALYSIS OF FOOD AND NATURAL PRODUCTS LABORATORY EXERCISE Determination of Volatile Substances Proof of Food Adulteration (method: gas chromatography with mass spectrometric detection) Exercise guarantor:
More informationU.S. EPA Method 8270 for multicomponent analyte determination
ENVIRONMENTAL application note U.S. EPA Method 8270 for multicomponent analyte determination Elaine A. LeMoine and Herman Hoberecht Introduction Multicomponent analytes are compounds that yield several
More informationAnalysis of Residual Solvents in Pharmaceuticals (USP<467>) with Shimadzu GC-2010 Plus and HS-10 Headspace Sampler
No. SSI-GC- Gas Chromatography No. GC- Analysis of Residual Solvents in Pharmaceuticals (USP) with Shimadzu GC- Plus and HS- Headspace Sampler Introduction Organic solvents are routinely used in manufacturing
More informationHighly Sensitive and Rugged GC/MS/MS Tool
Highly Sensitive and Rugged GC/MS/MS Tool For Pesticide Multiresidue Analysis in Food Samples Agilent 7 Series Triple Quadrupole GC/MS. The world s first MS/MS designed specifically for GC Analysis Introduction
More informationTOTALLY INNOVATIVE MULTIMODE AUTOSAMPLER NEW KONIK ROBOKROM Laboratory Gas Generators An Overview +8 OPERATIONAL MODES MAIN FEATURES
ROBOKROM 1 TOTALLY INNOVATIVE MULTIMODE AUTOSAMPLER NEW KONIK ROBOKROM Laboratory Gas Generators An Overview ROBOKROM 2 +8 OPERATIONAL MODES HRGC+HRGC-MS HRGC+HPLC-MS STATIC HEAD SPACE PURGE & TRAP SMPE
More informationVUV ANALYTICS VGA-100 GC DETECTOR Gas Chromatograph Agilent 6890 equipped with a 7683 model autosampler Restek 30m x 0.25mm x 0.
System Description The VUV PIONA + Analyzer is a VGA-100 Gas Chromatography (GC) detector configured for PIONA compound analysis by the VUV PIONA + method. The VUV PIONA + method uses relatively simple
More informationExercise in gas chromatography
AM0925 Exercise in gas chromatography Introduction The purpose of this exercise is to investigate the effect of different carrier gases (helium and nitrogen) on the Golay equation and the optimal carrier
More informationHarris: Quantitative Chemical Analysis, Eight Edition CHAPTER 23: GAS CHROMATOGRAPHY
Harris: Quantitative Chemical Analysis, Eight Edition CHAPTER 23: GAS CHROMATOGRAPHY Chapter 23. Gas Chromatography What did they eat in the year 1,000? GC of Cholesterol and other lipids extracted from
More informationIntroducing New Functionalities in Liquid Stationary Phases in GC Columns for Confirming Organic Volatile Impurity Testing in Pharmaceutical Products.
Introducing New Functionalities in Liquid Stationary Phases in GC Columns for Confirming Organic Volatile Impurity Testing in Pharmaceutical Products. CHRISTOPHER M. ENGLISH, CHRISTOPHER S. COX, FRANK
More informationUltra-Inert chemistry for Trace Level Analysis
Ultra-Inert chemistry for Trace Level Analysis Cikui Liang, Ph.D. Challenges and Needs of Today s Laboratories Challenges Qualification/quantification of trace samples Keep instrument up and running Needs
More informationEvaluation of Capillary Columns for General Performance Parameters
Evaluation of Capillary s for General Performance Parameters Application Authors Mitch Hastings, Eberhardt R. Kuhn and Allen K. Vickers Agilent Technologies, Inc 91 Blue Ravine Road Folsom, CA 95630 USA
More informationSolid Phase Micro Extraction of Flavor Compounds in Beer
Solid Phase Micro Extraction of Flavor s in Beer ANNE JUREK USEPA 524.2 Method Validation Using the Evolution Purge and Trap Concentrator and the Centurion WS Autosampler Application Note Environmental
More informationImproved Volatiles Analysis Using Static Headspace, the Agilent 5977B GC/MSD, and a High-efficiency Source
Improved Volatiles Analysis Using Static Headspace, the Agilent 5977B GC/MSD, and a High-efficiency Source Application Note Environmental Authors Peter Gautschi and Harry Prest Senior Application Scientist
More informationChromatographic Methods of Analysis Section: 5 Gas Chromatography (GC) Prof. Tarek A. Fayed
Chromatographic Methods of Analysis Section: 5 Gas Chromatography (GC) Prof. Tarek A. Fayed Gas Chromatography (GC) In gas chromatography, the sample is vaporized and injected onto the head of a chromatographic
More informationCHEM 429 / 529 Chemical Separation Techniques
CHEM 429 / 529 Chemical Separation Techniques Robert E. Synovec, Professor Department of Chemistry University of Washington Lecture 1 Course Introduction Goal Chromatography and Related Techniques Obtain
More informationGas chromatography. Advantages of GC. Disadvantages of GC
Advantages of GC Gas chromatography Fast analysis, typically minutes Effi cient, providing high resolution Sensitive, easily detecting ppm and often ppb Nondestructive, making possible on - line coupling;
More informationThe Suite for Environmental GC Analysis
The Suite for Environmental GC Analysis SGE Environmental GC Columns Performance Selectivity Delivery Promise www.sge.com SGE Environmental GC Columns The Suite for Environmental GC Analysis SGE GC Columns
More informationContinuous Improvement in Petroleum/Petrochemical Analysis HP s Family of Innovative PLOT Columns
Continuous Improvement in Petroleum/Petrochemical Analysis HP s Family of Innovative PLOT Columns Brochure Brief Porous Layer Open Tubular (PLOT) columns have been replacing traditional packed columns
More informationSimultaneous dual capillary column headspace GC with flame ionization confirmation and quantification according to USP <467> Application Note
Simultaneous dual capillary column headspace GC with flame ionization confirmation and quantification according to USP Application Note Joseph M. Levy Michael Kraft Abstract Agilent Equipment 7890A
More information2] The plate height in chromatography is best described as 2
9 Chromatography. General Topics 1] Explain the three major components of the van Deemter equation. Sketch a clearly labeled diagram describing each effect. What is the salient point of the van Deemter
More informationApplication. Gas Chromatography February Introduction
Ambient Headspace Analysis with the Agilent 7683 Automatic Liquid Sampler Application Gas Chromatography February 1998 Authors Matthew S. Klee and Chin Kai Meng Agilent Technologies, Inc. 2850 Centerville
More informationCourse goals: Course goals: Lecture 1 A brief introduction to chromatography. AM Quality parameters and optimization in Chromatography
Emqal module: M0925 - Quality parameters and optimization in is a separation technique used for quantification of mixtures of analytes Svein.mjos@kj.uib.no Exercises and lectures can be found at www.chrombox.org/emq
More information