Division of Teaching Labs Policies and Procedures

Transcription

1 Divisin f Teaching Labs Plicies and Prcedures When participating in labratry sessins in the Divisin f Teaching Labs (DTL) at the University f Trnt (UfT), Faculty f Medicine, there are several plicies and best practices that must be fllwed. Here at DTL we cnstantly strive t prvide the safest envirnment pssible. T this end we ask that each student read the fllwing plicies and prcedures. Keep in mind that as a UfT student, under the Cde f Student Cnduct, yu are expected t abide by these plicies and prcedures. PART ONE: GENERAL LAB POLICIES AND PROCEDURES GENERAL RULES 1. Always act in a respnsible manner in the lab. 2. Always be prepared by reading thrugh the lab instructins prir t cming t class. Always check-in with yur instructr befre prceeding with an experiment and if yu are unsure f any f the directins r hw t perate particular equipment. 3. Keep wrk areas clean and tidy at all times. Likewise keep the flr and aisles clear. Bring nly the necessary labratry materials (e.g. lab manual and ntebk) t yur bench. Other items (cats, backpacks, etc.) shuld be stred in the designated areas in the lab rm. 4. Always respect yur fellw students and ther persnnel in the lab. T help reduce the pssibility f accidents yu shuld at all times be cncentrating n what yu are ding, and at n time shuld yu engage in any frm f hrseplay. 5. Never cnsume fd r beverages r place any fd/beverage items n any lab surfaces including the Interlabs. Never dispse f fd r beverage waste in lab garbage bins. If yu wish t cnsume fd r beverages yu may d s ut in the hallways and dispse f the fd/beverage waste in the hallway garbage bins. 6. Dress apprpriately fr all lab activities by wearing clsed-te shes and lng pants. Always tie back lng/lse hair and secure any dangling jewellery. 7. Wear the apprpriate persnal prtective equipment, PPE, (e.g. glves, gggles, lab cat) as directed by yur instructr. A fully buttned up lab cat is required t participate in all wet lab sessins. Each student must bring their wn lab cat t the lab, which can be purchased frm the Bkstre r MedStres n campus. Stre yur lab cat in a plastic bag separate frm yur ther belngings. 8. Keep yur hands away frm yur face, eyes, muth and bdy. D nt handle any prtable devices, such as cellphnes, laptps, r ther devices with glved r unwashed hands. 9. Remve all PPE and wash yur hands with sap and water after perfrming experiments and befre exiting the lab rm. Use designated handwashing sinks ONLY fr washing hands, nt fr dispsing f reagents, rinsing glassware r cnducting experiments. 10. Clean up after the lab by: (a) dispsing f waste in designated cntainers; (b) remving all labels frm reusable glassware, then rinsing the glassware and placing it in the designated cntainers; (c) clearing yur lab bench and any Interlab space that yu ve used; and (d) rinsing and returning all equipment clean and in wrking rder t their riginal place. 11. If any equipment is brken r malfunctining, infrm yur instructr r the Interlab technician s that it can be repaired. 12. Never pipette by muth; nly use the rubber bulbs r pipette aids prvided. When using a pipette aid, yu must be extremely careful t nt draw any liquid int the pipette aid. 13. Never bring chemicals, bilgical rganisms, glassware r equipment int the Interlab except when necessary t use a piece f equipment lcated there (e.g. balance, spectrphtmeter, centrifuge, etc.) r t use the fumehd in the Interlab. If it is necessary t bring such items int the Interlab then the student must als bring it back t his/her lab bench after use. 14. Never transfer equipment, glassware, bilgical rganisms r chemicals frm ne lab t anther unless specified and supervised by the instructr. The instructr shuld infrm the Interlab technician f any equipment transfers. 15. When using equipment taken ff shelves r wagns return them t their riginal lcatin at the end f the lab sessin. 16. T reduce the pssibility f accidents, keep Interlab traffic t a minimum s that it des nt becme vercrwded. 17. Handle all glassware, sharps, rganisms and chemicals in a respectful manner and dispse f them prperly. Specific guidelines n hw t dispse f varius types f waste are described in Part Tw. 18. Let yur instructr knw if yu have any allergies r medical cnditins which may affect yur ability t cnduct an experiment r t receive treatment in the event f an accident r health incident. Last updated: Page 1 f 7

2 EMERGENCIES, ACCIDENTS & INJURIES Divisin f Teaching Labs Plicies and Prcedures 19. Knw the lcatin and perating prcedure f the eye wash statin within yur lab and emergency shwer in the Interlab. Yur instructr will shw yu hw t use an eye wash statin. 20. Knw the lcatin f the dust pan and brm, spill kit, and first aid kit. Yur TA/instructr will attend t spills, brken glassware and first aid; students are nt t clean up spills r attend t injuries. 21. Reprt any accident (spill, breakage, etc.), injury (cut, burn, etc.), r health incident (e.g. dizziness, fainting), t the instructr immediately, n matter hw trivial it may appear. 22. If a chemical splashes in yur eye(s), immediately flush with running water frm the eye wash statin fr at least 20 minutes. Ntify yur instructr immediately. 23. If a chemical splashes n yur skin, immediately flush with running water fr at least 20 minutes. Fr small spills (e.g. n yur hand) it wuld be apprpriate t use a sink. Use f the emergency shwer is usually nly necessary if a large amunt f a hazardus substance has splashed n yur bdy. If clthing is cntaminated it shuld be remved. Ntify yur instructr f the incident immediately. 24. Knw the lcatin f the fire alarm and emergency exits. 25. In case f emergency r fire drill, stay calm and fllw yur instructr s directins. Yu may be required t shut dwn certain equipment (e.g. Bunsen burners) befre exiting the lab rm. Leave all bags, cats, etc. in the lab rms and prceed in an rderly fashin alng with yur instructr t the nearest exit. Yur instructr will ensure that the lab rm is lcked. WORKING WITH OPEN FLAMES & HEATING SUBSTANCES 26. Always fllw yur instructr s directins n the prper use f Bunsen burners and ther heating devices (e.g. ht plates, high temperature water baths). 27. Exercise extreme cautin when using a Bunsen burner. Tie back lng/lse hair, lse jewellery, and ensure yu are a safe distance frm the flame at all times. D nt put any substance int the flame unless specifically instructed t d s. NEVER reach ver an expsed flame. NEVER leave a lit Bunsen burner unattended. 28. NEVER leave anything that is being heated at high temperature r is visibly reacting unattended. 29. Always turn the burner r ht plate ff when nt in use. 30. Exercise extreme cautin when transferring substances int and ut f biling water baths, heating blcks r micrwaves. 31. Prtect yur hands (e.g. use tngs, wear prtective glves) when handling glassware r metal bjects that have been heated. HANDLING REUSABLE GLASSWARE 32. Students must respect glassware as ptentially hazardus and dangerus items. Glassware must nly be handled fr its intended purpse. 33. Dirty reusable glassware MUST be placed in the apprpriately marked DIRTY glassware cntainers. DO NOT PUT DIRTY GLASSWARE BACK IN WITH CLEAN GLASSWARE. 34. Thrughly rinse glassware (if nn-hazardus) and remve all labels befre placing them in the dirty glassware bins. 35. Brken and dispsable glassware (including Pasteur pipettes), plastic cnsumables (e.g. tips), and items designated as sharps are t be dispsed f in the apprpriate cntainers as described in Part Tw. RECYCLING 36. In many instances waste generated within a labratry setting can be safely recycled thereby minimizing the envirnmental impact f yur wrk. Items that may be recycled include: glassware, plasticware, paper prducts, electrnics (tner, cmputer equipment, lab equipment), and batteries 37. Befre yu cnsider recycling any slid waste yu must ensure that it is NOT CONTAMINATED with any hazardus material; it has t be free f any bilgical, chemical, r radiactive cntaminatin. 38. All prducts must be triple rinsed befre they are placed int the designated recycling cntainer/bin. 39. Please check with yur instructr as t whether yu may recycle a particular item. Last updated: Page 2 f 7

3 Divisin f Teaching Labs Plicies and Prcedures PART TWO: PROPER HANDLING & WASTE DISPOSAL IN THE LAB CHEMICAL HANDLING AND DISPOSAL During the curse f a lab sessin yu may be required t dispense r handle hazardus reagents that may be reactive, pisnus, and/r crrsive. Please make sure that yu use apprpriate PPE and exercise precautins when handling such chemicals and dispse f them int the apprpriate cntainer as utlined belw. Nte that glassware and cnsumables that have bilgical r radiactive cntaminatin are treated differently (see sectins n Wrking with Organisms and Wrking with Radiistpes belw). When wrking with chemicals: Pay attentin t warning labels prvided n chemical bttles. Take nly as much chemical as yu need. Never return unused chemicals t their riginal cntainers. Never tuch r taste any chemicals. When smelling a chemical use the wafting technique. When dispsing f chemical waste: LIQUID CHEMICALS: Use designated bttles r beakers that will be setup and labelled in the lab. DO NOT fill cntainers past 75% f the ttal vlume. Sme chemicals can be dispsed f dwn the drain; befre ding s, verify with yur instructr that this is permitted. SOLID CHEMICALS: Use designated beakers that will be setup and labelled in the lab. LAB CONSUMABLES USED WITH CHEMICALS (EXCEPT MEDICAL SHARPS): Lab Cnsumables (LCs ) such as tips, micrtubes, Pasteur pipettes, that have cme int cntact with nn-hazardus chemicals are cllected in a Milkshake Cup at yur wrkbench and dispsed at the end f yur class in a designated cntainer. LCs such as plastic tubes, plastic 96-well plates, plastic syringes (nt needles) that have cme int cntact with nn-hazardus chemicals shuld be rinsed and cllected in a designated cntainer n the centre bench. These plastics will be recycled with the municipal waste. LCs used with hazardus material must be dispsed f in a GREEN PAIL, unless designated therwise by yur instructr. Nte that Pasteur pipettes are nt reused, therefre they must always be dispsed f in the GREEN PAIL. D nt place Pasteur pipettes int basins marked fr dirty pipettes (this refers t reusable glass pipettes nly). Remember t return the rubber bulb t the general labratry supplies tray. MEDICAL SHARPS USED WITH CHEMICALS: Razr blades, scalpels, needles, lancets and ther medical sharps are dispsed f in a YELLOW BIOHAZARD SHARPS CONTAINER ONLY! If yu are unsure r cnfused as t where smething shuld be dispsed f always ask yur instructr! Images f the varius dispsal cntainers can be fund at this website: Last updated: Page 3 f 7

4 WORKING WITH ANIMAL SAMPLES Divisin f Teaching Labs Plicies and Prcedures Overview: Animal wrk is typically cnducted in the Department f Cmparative Medicine s (DCM) facilities. Students will receive training frm DCM and must cmply with their rules and regulatins fr prper animal care. Fr animal experiments cnducted in DTL, students will receive hands-n training frm the Curse Crdinatr, teaching assistant, r a DCM staff member. Prcedures at DTL: Animal prducts are cllected in bidegradable GREEN BAGS. DO NOT place any ther materials such as glves, paper twels, tips, needles, etc., in these bags. Students are respnsible fr the surgical instruments that they use. All surgical instruments must be saked and washed after each use t prevent damage. Instructins will be prvided in the lab. Phtgraphing any animal wrk is nt permissible given the sensitive nature surrunding the use f animals in experiments. WORKING WITH HUMAN SAMPLES Wrking with and dispsing f human samples: Universal Precautins must be taken whenever cnducting experiments invlving human bld and any ther ptentially infectius material (e.g. saliva, urine, etc.). Since we cannt determine by eye whether such materials may be infectius, we must treat them as if they are infected and handle and dispse f them as ptentially infectius materials. These materials and all cntaminated waste generated during these experiments are dispsed f in YELLOW BIOHAZARD PAILS. In the case f an accident invlving human bld r ther ptentially infectius material: If yu are bleeding, ntify yur instructr immediately. D nt ask fellw classmates fr assistance s that we minimize the risk f expsure t thers. If yur injury allws, yu may be asked t help clean up any cntaminated areas. Anyne wh assists an injured party shuld wear glves t prtect themselves. All cntaminated areas must be prperly disinfected and infectius waste dispsed f in the YELLOW BIOHAZARD PAILS. WORKING IN A CONTAINMENT LEVEL 2 ROOM Rms that are zned as Cntainment Level 2 (CL2) have special rules: v Users f these rms may nt bring persnal items (e.g. backpacks, cats, etc.) int these rms. Designated strage rms will be prvided fr yu t stre yur persnal belngings. v Lab cats used in these rms must stay in these rms. At the end f the curse, yur lab cat will be disinfected and washed, and returned t yu. Currently, DTL rms 3282, 3284, 3379, 3381, 3383 and 3397 are designated as CL2 (indicated by signs n the drs). Last updated: Page 4 f 7

5 Divisin f Teaching Labs Plicies and Prcedures WORKING WITH MICROORGANISMS: RISK GROUP 1 When wrking with micrrganisms designated as Risk Grup 1 (RG1), special precautins must be taken t ensure the wrkspace is used and cleaned prperly and that all bilgically cntaminated waste is prperly dispsed f in the apprpriate cntainers. Please verify with yur instructr whether the bilgicals yu will be using are designated as RG1 r RG2. When wrking with RG1 rganisms: Wear the apprpriate PPE as directed by yur instructr Disinfect yur wrkbench BEFORE and AFTER use with either Dettl r 70% alchl When dispsing f RG1 waste: Dispse f RG1 bilgically cntaminated waste int apprpriate cntainers, using the fllwing rules: GLASSWARE REUSABLE GLASS PIPETTES: Bilgically cntaminated reusable glass pipettes (nt Pasteur pipettes) shuld be placed in METAL PANS OR BLEACH-FILLED JARS as instructed by yur instructr OTHER REUSABLE GLASSWARE: Other bilgically cntaminated reusable glassware (e.g. beakers, flasks, etc. ) shuld be placed in the designated METAL BUCKETS. MEDIA PLATES: Place slid media plates in the designated METAL BUCKETS. LAB CONSUMABLES LAB CONSUMABLES: Bilgically cntaminated cnsumables (e.g. tips, Pasteur pipettes, but NOT medical sharps such as needles and razrs) are dispsed f in a receptacle lined with a RED BIOHAZARD BAG OR METAL CONTAINER MEDICAL SHARPS: Razr blades, scalpels, needles, lancets and ther sharps are dispsed f in a YELLOW BIOHAZARD SHARPS CONTAINER ONLY! D NOT attempt t remve a needle frm a syringe r recap the needle; place the entire assembly in the sharps cntainer. LIQUID WASTE: Use designated bttles r beakers that will be setup and labelled in the lab. DO NOT fill cntainers past 75% f the ttal vlume. When using bisafety cabinets (BSCs) with RG1 rganisms: Disinfect the wrkspace befre and after use Tidy up Dispse f any materials (tips, pipettes, etc.) as directed by yur instructr Nte that standard BSC prcedure is fr nly ne user t wrk at a BSC at a time. Fr teaching purpses, we may allw tw students t wrk simultaneusly at a larger BSC fr RISK GROUP 1 WORK ONLY. Last updated: Page 5 f 7

6 Divisin f Teaching Labs Plicies and Prcedures WORKING WITH MICROORGANISMS: RISK GROUP 2 When wrking with micrrganisms designated as Risk Grup 2 (RG2) special precautins must be taken t ensure the wrkspace is used and cleaned prperly and that all RG2 waste is prperly dispsed f in the apprpriate cntainers. Please verify with yur instructr whether the bilgicals yu will be using are designated as RG1 r RG2. When wrking with RG2 rganisms: Wear the apprpriate PPE as directed by yur instructr Disinfect yur wrkbench BEFORE and AFTER use with either Dettl r 70% alchl When dispsing f RG2 waste: ALL RG2 WASTE: All waste frm RG2 rganisms (plates, tips, lab cnsumables, etc.) MUST BE dispsed f in YELLOW BIOHAZARD PAILS. MEDICAL SHARPS: Razr blades, scalpels, needles, lancets and ther sharps are dispsed f in a YELLOW BIOHAZARD SHARPS CONTAINER ONLY! D NOT attempt t remve a needle frm a syringe r recap the needle; place the entire assembly in the sharps cntainer. When using bisafety cabinets (BSCs) with RG2 rganisms: Disinfect the wrkspace befre and after use using the 70% alchl prvided in spray bttles Tidy up Dispse f any materials (tips, pipettes, etc.) as directed by yur instructr Nte that bisafety regulatins stipulate that ONLY ONE USER SHOULD WORK AT A BSC AT A TIME WHEN CONDUCTING WORK WITH RISK GROUP 2 ORGANISMS. Students must cmply with this regulatin when wrking with Risk Grup 2 rganisms in ur BSCs. Last updated: Page 6 f 7

7 Divisin f Teaching Labs Plicies and Prcedures WORKING WITH RADIOISOTOPES Nte: Fr , n curses run in DTL will be using radiistpes. When wrking with radiactive substances: Wear glves and a lab cat at ALL TIMES ONLY wrk ver benches that have been cvered with white absrbent paper ONLY use equipment/tls that have been specifically designated fr radiactive wrk, such as MARKED centrifuges, MARKED shakers, MARKED pipettrs, etc. Understand the prperties, risks and prper handling prcedures assciated with the particular radiistpes yu are using; ask yur instructr if yu are at any time unsure abut the prper prcedures. If any equipment r wrkspace becmes cntaminated with a radiactive substance infrm yur instructr immediately s that it can be remved frm use and decntaminated. If an accident r injury ccurs while wrking with a radiactive substance infrm yur instructr immediately s that the apprpriate assessment and treatment can be perfrmed. When dispsing f radiactive substances: Dispse f radiactive waste int the apprpriately MARKED cntainers: LIQUIDS: Carefully pur r pipette radiactive liquids int the apprpriate CELITE CONTAINER nte that specific cntainers are used depending n the half-life f the radiistpe. Be sure nt t verfill these cntainers. D nt put any slid waste in these cntainers. SMALL LAB CONSUMABLES (E.G. TIPS, TUBES): Place in the prvided empty glve bxes n the bench as yu are wrking. When yu have cmpleted yur wrk (r when the bx is full), seal the glve bx with tape and dispse f it in the YELLOW RADIOACTIVE WASTE BIN. OTHER DISPOSABLE SOLIDS: Dispse f slids (e.g. glves, paper twels, etc.) in the YELLOW RADIOACTIVE WASTE BIN. GLASSWARE: Befre they can be placed in the dirty glassware bins, radiactively-cntaminated glassware must be thrughly rinsed using Dettl slutin and all rinse slutins dispsed f as radiactive liquid waste in the apprpriate celite cntainer. D NOT dispse f the rinse slutins dwn the drain! See pictures f cntainers at: Last updated: Page 7 f 7