Influence of the growth stage of industrial hemp on the yield formation in relation to certain fibre quality traits

Transcription

1 Industrial Crops and Products 13 (2001) Influence of the growth stage of industrial hemp on the yield formation in relation to certain fibre quality traits V. Mediavilla a, *, M. Leupin a, A. Keller b a Swiss Federal Research Station for Agroecology and Agriculture, P.O. Box, CH-8046 Zurich, Switzerland b Swiss Federal Research Station for Agricultural Economics and Engineering, CH-8356 Tanikon, Switzerland Received 6 January 2000; received in revised form 12 April 2000; accepted 14 April 2000 Abstract To study the influence of the stage of growth of industrial hemp (Cannabis sati a L.) on yield formation and fibre morphology, a field trial was carried out in Switzerland in Different harvests took place at 7 14 day intervals, from the vegetative stage of growth to the senescence of the crop. Total yield and its components, fibre content and the frequency of primary and secondary fibres as well as the exact stage of growth were determined in male and female plants. Stem, bark and fibre yield reached their maximum at the time of flowering of the male plants ( technical maturity ). Maximum stem yield amounted to 14.8 tons of dry matter (DM) per hectare. Bark yield showed a development similar to that of the stem yield and reached 5.8 tons DM/ha. Fibre yield was highly correlated with stem and bark development and also reached its maximum at the time of flowering of the male plants (yield: 4.1 tons DM/ha). During the vegetative phase, primary fibres were first created and then filled. The peak of the stem and fibre yield at the male plant flowering stage was probably caused by an increase in production and lead to a filling of secondary fibres. After that, and because of their characteristics, secondary fibres may cause a decrease in bark quality. With regard to fibre production, the upper third of the stem did not account for much fibre yield Elsevier Science B.V. All rights reserved. Keywords: Cannabis sati a L.; Fiber hemp; Bark; Growth; Harvest time; Fiber quality; Primary and secondary bark fibers 1. Introduction Hemp (Cannabis sati a L.) can be used in many ways. The utilisation of fibres, seeds, oil, inflorescences, essential oils, and secondary compounds * Corresponding author. Tel.: ; fax: address: vito.mediavilla@fal.admin.ch (V. Mediavilla). such as THC have been investigated. Similarly to other crops harvest time plays a considerable role in industrial hemp yield and quality (Bòcsa and Karus, 1997, pp ). Different criteria for the determination of the optimal harvesting time for fibre hemp are applied in different countries: calendar date, stem colour, drop of the internodia-leaves and of course, crop growing stage (Fédération Nationale des Producteurs de Chanvre, 1984; Lohmeyer, 1997). With /01/$ - see front matter 2001 Elsevier Science B.V. All rights reserved. PII: S (00)

2 50 V. Media illa et al. / Industrial Crops and Products 13 (2001) monoecious varieties technical maturity for fibre production is the peak of flowering, that is the time when most of the bracts are formed. For dioecious varieties, technical maturity coincides with the time when most of the staminate flowers on male plants are open and occurs usually at the beginning of the bract formation on female plants (Bòcsa and Karus, 1997, p. 105). Depending on end-use, fibre quality is defined differently: For the production of pulp and paper, no significant influence on bast fibre strength and thermo mechanical pulping handsheet strength properties could be observed until 2 months after flowering (van der Veen, 1995). However, late harvest may cause problems with the harvest machinery (Lohmeyer, 1997). For the production of textiles and high-tech composites, fibre characteristics such as strength, length, diameter, chemistry, and homogeneity are influenced considerably by harvest time. Schulz (1998) brought the influence of harvest time into relationship with fibre yield, strength, elasticity, and the finesse of hemp fibres. Rowell and Stout (1998) reported changes in chemical composition (e.g. cellulose, sugars and lignin) and fibre characteristics (e.g. length and width) of jute and kenaf at different stages of plant growth. Morrison et al. (1999) describe the chemical characterisation and the retting of maturing kenaf stems. Hemp contains three different kinds of fibres: core fibres are located inside the vascular cambium ( mm long), primary bast fibres are situated outside the vascular cambium (about 20 mm long) and secondary bast fibres rise from the prodesmogen (about 2 mm long) (van der Werf et al., 1994). For long fibre uses (e.g. textiles and composites), the stem value depends primarily on the proportion of bark fibres. It is common knowledge that a high bark yield and bast fibre content as well as a low secondary bast fibre content are advantageous for the production of textiles. Within the scope of examining the production of hemp fibres for textiles and composites we studied an enzymatic degumming process (Leupin, 1998). Based on this process we analysed the influence of harvest time on fibre yield and quality, stem proportion and plant sex. In this paper we will describe the results on aboveground dry matter production and distribution as well as filling degree and the cross-section area of primary and secondary fibres. The consequences for chemical and physical fibre properties have been laid down by Keller et al. (2001). 2. Methods Fibre hemp of the Kompolti variety, was sown on ando-eutric cambisol soil (International Society of Soil Science, 1986) at a rate of 60 kg/ha in Hettlingen (Switzerland) near the town of Winterthur on the 2nd of May 1997 (460 m above sea level; 8 45 E, N). The experiment involved a complete randomised block design with four replications. In consideration of the cultural precedent (beans), nitrogen fertilisation was reduced to 60 kg/ha N. Weed and pest control were not carried out. Precipitation was well distributed over the season (beginning of May to the end of September) and reached a total level of 436 mm (Fig. 1). With each harvest (Table 1), in every replicate, those plant parts 1 m 2 above-ground were harvested and the number of plants was determined. For each replication, leaf and stem dry matter content was measured (sample of ten plants dried at 100 C for 24 h). For each replication, the stage of growth was determined by means of a randomised sample of 30 plants, according to Mediavilla et al. (1998b) (Table 2). Crop stages were defined, assuming that 50% of plants had reached the same stage of growth. For the determination of bark and fibre content, a randomised sample of 25 plants per replication was subdivided, if evident, into male and female plants into three equal parts, namely top, middle, and bottom. The bark of all three parts was separated manually from the fresh stems and dried at 40 C. The unlignified apex was discarded. Taking into consideration plant density, stem yield, the fibre content of each stem and if evident the male/female ratio, fibre yield distribution per surface basis was calculated. After the 6th harvest, it was no longer possible to separate the bark from the stems of the top part.

3 V. Media illa et al. / Industrial Crops and Products 13 (2001) The fibre content of the bark was determined in ground samples by means of a NIR Systems scanning monochromator, model Calibration equations were obtained with modified partial least squares regression (MPLS; Infrasoft International, Silver Spring, MD, USA). The calibration set of samples was determined gravimetrically by cutting 2.5 g of bark into cm long pieces and autoclaving them in 25 ml alkaline solution (6% (w/v) KOH/0.4% )w/v) Na 2 SiO 3 / 0.3% (w/v) Na 4 P 2 O 7 ) at 121 C for 30 min. After cooling down, the fibres were washed thoroughly with hot and cold water, neutralised with 1% acetic acid and washed again with water, prior to being dried at 110 C. For each replication microscopical analyses of fibres were made from a sample of five plants. Samples taken from a transverse section in the middle of the stem were first fixed in a formaldehyde (35%) acetic acid ethanol water solution (10:5:50:35) for at least 24 h, then dehydrated with an ethanol tert-butyl alcohol series and finally embedded in paraffin (Gerlach, 1984). Cross-sections were cut at m from fixed paraffin embedded material at 5 m, using a Sorvall JB-4 hand-microtome. All sections were stained with Toluidine blue. For each harvesting date microscopical pictures of the plant cross-sections were taken in triplicate. Using the photographs, the amount of primary and secondary fibre cells was counted, the state of ripeness evaluated, their surface measured and average cell size calculated. The stage of fibre maturity was differentiated, depending on the degree of ripeness in low (non-filled to low-filled), middle-filled and full-filled (well filled to ripe) cells. No fibre maturity or cross-section areas could be determined at the last harvest. Fig. 1. Daily average temperature and precipitation in the trial location during the period of growth (DOY). Table 1 Harvest times Harvest c Date 14 July 28 July 4 August 11 August 18 August 25 August 3 September 17 September 1 October DOY

4 52 V. Media illa et al. / Industrial Crops and Products 13 (2001) Table 2 Definitions and codes of growth stages of C. sati a L. dioecious plants according to Mediavilla et al. (1998b) Code Definition Remarks Germination and emergence 0000 Dry seed 0001 Radicle apparent 0002 Emergence of hypocotyl 0003 Cotyledons unfolded Vegetati e stage refers to main stem; lea es are considered as unfolded when leaflets are at least 1 cm long st leaf pair 1 leaflet nd leaf pair 3 leaflets rd leaf pair 5 leaflets th leaf pair 7 leaflets th leaf pair 10xx nth leaf pair xx=2(nth leaf pair) Flowering and seed formation refers to the main stem including branches 2000 GV point Change of phyllotaxis on the main stem from opposite to alternate; distance between petioles of alternate leaves at least 0.5 cm 2001 Flower Sex nearly indistinguishable primordia Male plant 2100 Flower First closed staminate flowers 2101 formation Beginning of First opened staminate flowers flowering 2102 Flowering 50% opened staminate flowers 2103 End of 95% of staminate flowers open or withered flowering Female plant 2200 Flower First female flowers; bract with no pistils 2201 formation Beginning of Styles on first female flowers flowering 2202 Flowering 50% of bracts formed 2203 Beginning of First seeds hard seed maturity 2204 Seed maturity 50% of seeds hard 2205 End of seed 95% of seeds hard or shattered maturity Senescence 3001 Leaf desiccation Leaves dry 3002 Stem desiccation Leaves dropped 3003 Stem Bast fibres free decomposition

5 V. Media illa et al. / Industrial Crops and Products 13 (2001) Results and discussion Average plant density of the different harvests was approximately 170 plants/m 2 (Fig. 2). This is a typical density for fibre production after day of the year (DOY) 200 for Central Europe (Meijer et al., 1995). We assume that a decrease of plant density took place before the first harvest, that is during the first phases of the vegetative stages. Stem yield increased rapidly during the vegetative growing stages (growth codes , according to Mediavilla et al. (1998b)) until the GV point (change of the phyllotaxis) was reached on DOY 216 (code 2000). Maximum stem yield was 14.8 tons of dry matter (DM) per hectare at DOY 246, at the end of male flowering (code 2103) and the peak of female flowering (code 2202), which is described as technical maturity (Fig. 2). After that the yield declined due to the senescence: first of the male, then of the female plants (Fig. 2). For different varieties a similar growth pattern with a maximum of stem yield at flowering was described (Meijer et al., 1995; Mediavilla et al., 1998a). Bark yield showed a development similar to stem yield and reached 5.8 tons DM/ha on DOY 246 (Fig. 2). Fibre yield was highly correlated with stem and bark development and reached the maximum at the same time (4.1 tons DM/ha) (Fig. 3). Approximate 54% of the fibres were located in the bottom part, 34% in the middle, and only 12% in the top part of the stem. These results correspond to those recorded by van der Werf et al. (1994) who pointed out that under comparable growth and climatic conditions, 11 12% of bark fibres are located in the upper 30% of the stem. During flower formation, fibre yield increase was very pronounced at the bottom of the stem (Fig. 3). After flowering (DOY 246) fibre yield decreased in the whole stem. Female plants showed a higher fibre yield than male plants, not because of a higher fibre content, but due to the higher female/ male ratio in the crop (53:47%). During the season of growth, primary fibres in the middle of the stem showed different degrees of ripeness. At the 6th leaf pair stage (DOY 195), 81% of all fibres were almost empty. Two weeks later, at the 8th leaf pair stage, the beginning of the fibre filling process could be recorded (Fig. 4). This was when the filling of 44% of the fibres was low and that of 33% was medium. From the beginning of flower formation the total fibres filling rate was more or less continuous. Male and female filling rates did not show any distinct pattern. Fig. 2. Growth pattern of above-ground dry matter production, distribution of dry matter between the plant parts and plant density during the season of growth. Growth stages according to Mediavilla et al. (1998b).

6 54 V. Media illa et al. / Industrial Crops and Products 13 (2001) Fig. 3. Growth pattern of fibre yield and distribution in top, middle and ground of the stems of male and female plants during the growth season. Growth stages according to Mediavilla et al. (1998b). Fig. 4. Filling degree of fibres distributed in top, middle and ground of the stems of male and female plants during the season of growth. Growth stages according to Mediavilla et al. (1998b). During the vegetative growth of the plants, in the middle of the stems, only primary fibres could be identified (Fig. 5). With the flowering induction, secondary fibres appeared in the bark and commanded up to 45% of the fibre area at DOY 230. In contrast to male plants, after the GV point, the female plants showed an abrupt increase in secondary fibres. Subsequently, no significant differences between male and female plants could be determined. At the time of the highest fibre yield (DOY 246), 65% of the section fibre area in the middle of the stems were from primary fibres. During flowering, van der Werf et al. (1994) reported that given comparable cli-

7 V. Media illa et al. / Industrial Crops and Products 13 (2001) Fig. 5. Cross-section of primary and secondary fibre cells distributed in top, middle and ground of the stems of male and female plants during the season of growth. Growth stages according to Mediavilla et al. (1998b). matic conditions a similar primary fibre content of 57 68% (weight) could be determined in the middle of the stem. 4. Conclusions Stem, bark and fibre yield are clearly correlated with the growth stage of the crop. Harvesting dioecious hemp at the time of its technical maturity maximises stem and fibre yield. The process of bark fibre formation is complex and also strongly related with the growth pattern of the plant. During vegetative growth and until flower formation, primary fibres are first created and then filled. With the induction of the generative phase, secondary fibres, especially at the bottom of the stem, are built. Peaks of stem yield and fibre yield at technical maturity are probably caused by an increase in the production of secondary fibres. Because of their characteristics (shorter, thicker walls and high lignin content (Hoffmann, 1961)) bark quality may decrease. In female plants and probably also in monoecious plants the formation of secondary fibres begins earlier and could contribute to a faster decrease of bark quality. In terms of fibre yield, the top third of the stem did not account for much and can, therefore, be used for other purposes. In contrast to the top, the bottom of the stem is very rich in fibres and should be cut as low as possible, given the fact that it has no influence on fibre quality. Acknowledgements The authors wish to thank Franca Ciocco and Nicolas Mo-Costabella for their technical support. References Bòcsa, I., Karus, M., Der Hanfanbau Botanik, Sorten, Anbau und Ernte, 1st edn. C.F. Müller, Köln, Germany. Fédération Nationale des Producteurs de Chanvre, La Culture non Battue du Chanvre Monoïque. Fédération Nationale des Producteurs de Chanvre, Le Mans, France. Gerlach, D., Botanische Mikrotechnik: Eine Einführung. Georg Thieme, Stuttgart. Hoffmann, W., Hanf, Cannabis sativa L. Handbuch der Pflanzenzüchtung, vol. 5. Paul Parey, Berlin. International Society of Soil Science, Soil map of Middle Europe 1: Wageningen, The Netherlands.

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