NELUMBO A CULTIV AR OF PART 1: THE NAME OF THE CULTIVAR. different from above. Date named 6 Nameandaddressof Introducerif -

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1 1 "' ~ ApPLICATION TO REGISTER INTERNAllONAL WATERULYAND WATER GARDENING SOCIETY A CULTIV AR OF NELUMBO Appointed by the International Society for Horticultural Science (ISHS) as the International Cultivar Registration Authority (ICRA) for NELUMBO Please submit this form and all correspondence to: Auburn University Horticulture Lotus Project Dr. Ken Tilt 101 Funchess Hall Auburn University Auburn, AL Please fill out as completely as possible. Send form attached to totiltken@auburn.edu or to the address above. Phone office: or cell: PART 1: THE NAME OF THE CULTIVAR 1 State the genus ofthe cultivar Nelumbo 2 State the subspecies or hybrid(s) to Ne/umbo nucifera cv. Rachinee X Ne/umbo /utea which the cultivar belongs (ifknown) 3 State the proposed cultivar name Chandrakomen 4 Name and address of Originator Mr.Vichai Puripunyavanich Thailand Institute ofnuc1ear Technology 9/9 Moo7, Saimoon, Ongkarak, Nakomnayok 26120, Thailand Tel (66) Fax. (66) Date originated May 18, 2008 Name and address of Nominant if - differentfromabove Date named 6 Nameandaddressof Introducerif - different from above Date introduced

2 Name and address (plus phone/fax/ if available) of Registrant if different ITomabove 8 Ifthe cultivar name is being registered Thailand Institute of Nuclear Technology on behalf of an organization, give 9/9 Mo07, Saimoon, Ongkarak, name and address of that organization. Nakornnayok 26120, Thailand Tel (66) Fax. (66) I Has the Originator given permission for the cultivar to be registered under the proposed name? 10 I As far as you know has the name already been published in a dated publication together with a description? 11 I If the answer to 10 is "YES" please give details of the earliest publication and enclose a copy or photocopy of the relevant pages (including evidence of date) with this application form. 12 I As far as you know has the cultivar ever been subject to application for, or grant of, a Patent or Plant Breeders Rights. 13 I Ifthe answer to 12 is "YES" please give details of the first such issuing authority and any grant number (s) issued. Grant Number Issued by 14 I As far as you know is the cultivar being sold under a trade designation ('selling name') for marketing purposes instead of the cultivar name? 15 I If your answer to 14 is "YES" please list any such trade designations together with any other information you may have. 16 IIs the cultivar marketed under a trademark which has been formally registered by a trademark authority and entitled to bear the 1.7 I Is the cultivar marketed under a non-registered trademark denoted by the sign TM? 18 IIf the answerto 16or17 is "YES" please give name and address of the trademark owner (for registered trademarks), the issuing authority, and any grant number (if known). Circle: Circle: YES (NO Circle: YES \. N Circle: YES l NO.

3 ""',c".-..'~' 19 If the cultivar is named after someone living or dead, have Circle: YES NO you received permission from that person (or his/her legal (not applicable) representative) to register the name? 20 If it is not obvious, please state the Chandra (in Thai) = Moon or Moon light name derivation (meaning) of your Komen (in Thai) = Red sapphire proposed name Has a Standard been deposited for inspection in a Circle:- NO recognized herbarium? 22 If the answer to 21 is "YES" please Queen Sirikit Botanical Garden, Chiangmai, Thailand. list the herbarium and any others maintaining duplicates. PART 2: HISTORY OF THE CUL TIVAR 23 If the cultivarwas foundgrowingin - the wildpleasegiveoriginallocation whereit was found Was the cultivar selected from a cultivated environment? Circle: YES 25 Ifthe answer 24 was "YES" please state the name of the parents if known. - Seed (maternal) parent Pollen (paternal) parent Ne/umbo nucifera cv.rachinee (pink lotus) Ne/umbo /utea (mutant yellow lotus which can flower in Thailand.) 26 Name and address of Raiser or Mr.Vichai Puripunyavanich Hybridizer Thailand Institute of Nuclear Technology 9/9 Mo07, Saimoon, Ongkarak, Nakomnayok 26120, Thailand Tel (66) Fax. (66) Year of raising / hybridizing 2007 (flowered in 2008) Was the cultivar derived from a sport or chance seedling of Circle: YES another plant? 28 If the answer to 27 is "YES" please state the name of the - 29 parentplant. If the cultivarwas receivedas an unnamedseedlingor - plant where did it come from? (i.e. plant sale, etc.)

4 PART 3: DESCRIPTION OF THE CULTIVAR In order to complete registration of a cultivar an accurate description is required. This may include: a written description, photos of the flower and other diagnostic features, filling out the following section, or all ofthe above. (Digital Photos are preferable so they can be posted on-line) 30 What are the distinctive characteristics 1. The first-day blooming flower is of a pink petal of the new cultivar? shade with yellow color at the base. In the 2ndand 3rdblooming day, the pink color gradually recedes from the base toward the tip, while the yellow color expands from the base to the tip. On the last blooming day, the petal color is yellow with pink color at the very tip. 2. Plant size is very large, approximately cm in height. 31 What existing cultivar does the new Ne/umbo nucifera cv. Rachinee cultivar most resemble? 32 In what way is the cultivar distinct 1. The petal colors are pink and yellow, while from that in 32? Rachinee's petal colors are pink and white. 2. On the last blooming day, the petals have lost almost all of its pinkness and become yellow, while Rahinee's petals can still be recognized as pink and white. 3. The plant flowers only from May through October, while Rachinee can flower all year round. 4. The plant undergoes dormancy in December (when it sheds all its leaves), while Rachinee does not undergo dormancy. 5. The plant fails to yield viable seeds upon selffertilization, while Rachinee can yield a good amount of viable seeds. 6. Viable seeds from cross-fertilization are rare, while in Rachinee seeds from cross-fertilization are mostly viable. 33 Please indicate the color of the petals 1st dayblooming (see notes) Red-purple Group, no.62 A (petal tip) Red-purple Group, no.62 C (mid petal) White Group, no. 155 D (above petal base) Yellow Group, no.12b (petal base) 2ndday blooming Red-purple Group, no.58 D (petal tip) White Group, no. 155 B (mid petal) White Group, no. 155 B (above petal base) Yellow Group, noaa (petal base)

5 3rd(and 4th)dayblooming Red-purple Group, no.58 B (at the very tip of petal) Yellow Group, no.4d (mid petal) Yellow Group, no. 3C (above petal base) Yellow Group, no. 3C (petal base) Color chart: The Royal Horticultural Society, UK. 34 Additional colors on margins or veins Only1st daybloomingflower of petals Yellow-Green Group, no.144c (veins) 35 Is the color changeable over the life of Yes (detail in 33) the flower? 36 Flower diameter 30 centimeters blooming size. 37 Petal number petals 38 Receptacle color yellow 39 Receptacle diameter (at anthesis) centimeters 40 Carpel number Are the carpels (select one) Completely enclosed in the receptacle 42 Emergence height of flower above centimeters water 43 Leaf diameter centimeters 44 Emergent height of leaf above water centimeters /" 45 Tuber type (circle one) rmal tdible type Tuber forms a starchy dormant tuber Circle NO 47 Please write any other information you would like the Registrar to publish, sign and date.

6 Published paper: Proceedings of the sixth national lotus and water lily symposium, 31 July- 1 August pp InterspecificLine Breedingin Lotus(Nelumbo nuciferax Nelumbo lutea) L ' 2 K k a-ongsri VichaiPuripunyavanich1Woranuch 1 Thailand Institute,of Nuclear Technology (Public ana parn-rroanslrlcnalt Organization)2QueenSirikitBotaniAcg~ra8rn. The new two hybrid lotus lines were observed in Queen Sirikit Botanic Garden, Chiang Mai. The first line was the progeny of big a large 'Pathum' (Rachinee) X 'Mutant Yellow Lotus' and the second line came from a small 'Pathum' X 'Mutant Yellow Lotus'. The flower size of the first line was bigger than the second line. The first-day blooming flower was of a pink petal shade which gradually turned into a creamy yellow shade in the last blooming day. The second line was different. The petal shade was pinkish red from middle to tip with a creamy yellow shade near the petal base during blooming days and turned into a light creamy pink shade in the lastday. Note: photographs are attached in the next page. Vichai Puripunyavanich (Signature of Registrant) Dec I, 2008 (Date).is~~lffc>ttI{6f~ ".J~.~gi~tr4r'~"J!~~ on!: Denomination Class: Cultivar Epithet: Author & bib!. Citation:

7 ,., ~ Chandrakomen (left) flower, 1st day blooming;rightflower, 3rdday blooming). ~ Chandrakomen (left)comparedwith Rachinee(right) in 3rd