PLANT-MICROBE- INSECT INTERACTION 정혜정 조승우 정휘정

Transcription

1 PLANT-MICROBE- INSECT INTERACTION 정혜정 조승우 정휘정

2 Index Insect gut bacteria Phytoplasma BT toxin

3 PLANT-MICROBE-INSECT INTERACTION Insect gut bacteria

4 Plant-insect interaction influenced by insect gut bacteria

5 Plant defense against insect Herbivores Phytoalexin Pisatin Capsidiol

6 Insect gut bacteria overcome plant defense system 꿀벌 (Apis mellifera) 은벌꿀과꽃가루를섭취 꿀벌의장내미생물은 social contact 를통해서다른벌로 horizontal gene transfer 됨 Gilliamella apicola 의 pectinase

7 Insect gut bacteria overcome plant defense system The mountain pine beetles (Dendroctonus ponderosae) 소나무는 terpenoid 분비 딱정벌레장내미생물군집이 terpene degradation 을유도 Terpenoid-based defense 에저항

8 Antimicrobial activity against plant pathogenic fungi a A. solani, b B. cinerea, c C. gloeosporioides, d F. oxysporum, e P. capcisi A: only fungi; B: Ps. aeruginosa and fungi dual culture

9 Antimicrobial activity against plant pathogenic fungi Control effect of Pseudomonas aeruginosa against Colletotrichum gloeosporioides on chilli at 25, 10 days after spot experiments. (A: treat DW, C. gloeosporioides spray, B: only C. gloeosporioides spray, C: treat Ps. aeruginosa suspension cfu/ml)

10 Antimicrobial activity against plant pathogenic fungi Control effect of Pseudomonas aeruginosa against Colletotrichum gloeosporioides on chilli at 25, 15 days after spot experiments. (B: treat LB media, C. gloeosporioides spot; C: treat fungicide(haibichi), C. gloeosporioides spot; F: treat Ps. aeruginosa, C. gloeosporioides spot)

11 Insect gut bacteria induce plant defense

12 Insect gut bacteria induce plant defense Fall armyworm gut bacteria 토마토에처리시 Pantoea ananatis 는곤충에 peroxidase activity 를향상 Klebsiella sp. 는 trypsin proteinase inhibitor 분비증가

13 Insect gut bacteria induce plant defense Maize 에처리시 대부분의균에의해 Maize proteinase inhibitor gene expression 이크게향상 P.ananatis 균주를처리한잎에서 HR 반응이일어나서 cell death 발생

14 PLANT-MICROBE-INSECT INTERACTION Phytoplasma

15 Phytoplasmas through insect vector affect plant

16 phytoplasma 란? Phytoplasma 란식물 ( 植物 ) 관련마이코플라스마의새로운명칭. 식물의체관조직혹은식물숙주전이를위한매개충내에기생하는절대적기생미생물.

17 전이

18 Phytoplasma as plant pathogens 가지수를많게함 키가작아지게함 꽃이피어야할기관이꽃이안핌 잎이나열매를노랗게함 고사

19 엽화 [Phyllody]

20 Mechanism of Effector (virulence) proteins - phyllogens

21 빗자루병 [Witches broom]

22 Mechanism of Effector (virulence) proteins - tengu-su inducer (TENGU)

23 대추나무빗자루병 [ 오갈병 ]

24 Phytoplasma as desirable plant pathogens 포인세티아 가지수를많게한다! 유포르비아포겐스

25 Commercial poinsettias owe their lushness to a bacterial infection

26 PLANT-MICROBE-INSECT INTERACTION BT toxin

27 Plant-treated Bacillus thuringiensis kill insects

28 INTRO 미생물농약이란? Bacillus thuringiensis

29 INTRO Biopesticide What is BT? BT stands for Bacillus thuringiensis BT is discovered by Ishiwatari in Gram positive, soil bacterium, spore forming bacterium.

30 INTRO Bacillus thuringiensis During sporulation, Bt strain produce crystal proteins which is insecticidal δ-endotoxin (called Cry proteins) Crystal proteins toxins have specific activities against orders Lepidoptera( 나비목 ), Diptera( 파리목 ), Coleoptera( 딱정벌레목 ), Hymenopter( 벌목 ) and nematode( 선충 )

31 MECHANISM 1 Insects ingest toxin Crystals 2 Their alkaline digestive tracts make Crystals soluble and amenable to being cut with protease 3 The toxin is then inserted into the insect gut cell membrane, forming a pore

32 MECHANISM 벼해충흑명나방

33 MECHANISM Use of Bt genes in genetic engineering of plants for pest control

34 APPLICATION 감자해충콜로라도감자잎벌레 (Leptinotarsa decemlineata) 옥수수해충조명나방유충 (Ostrinia furnacalis) 목화해충목화명나방유충 (Haritalodes derogate)

35 APPLICATION 해충으로인한옥수수피해

36 APPLICATION Left: Bt corn Right: Non Bt corn

37 APPLICATION Bt cotton in India the area planted worldwide to genetically engineered Bt crops

38 Reference 오산나 서미자 윤영남 유용만, 2015, 식물병원성곰팡이에대한곤충장내세균의항균활성, 농약과학회지 제 19 권제 1 호, 농약과학회, pp Abdul Rashid War, 2012, Mechanisms of Plant Defense against Insect Herbivores, Plant Signaling & Behavior Vol. 7 No. 10, Landes Bioscience, pp Allison K. Hansen, 2014, The impact of microbial symbionts on host plant utilization by herbivorous insects, Molecular Ecology, pp Flor E. Acevedo, 2017, Fall Armyworm-Associated Gut Bacteria Modulate Plant Defense Responses, Molecular plant-microbe interactions Vol. 30, No. 2, The American Phytopathological Society, pp

39 Reference 김경희 박재영 차주훈 전치성 홍성준 김영호 허재선 고영진, 2011, 오이흰가루병방제용미생물농약의혼용에의한오이주요곰팡이병의방제효과, 농약과학회지 제 15 권제 3 호, pp 김의나, 2003, Bacillus thuringiensis 분리균의특성, 건국대학교대학원 김진수, 2009, 흑명나방에살충성이있는 Bacillus thuringiensis 균주의분리및특성조사, 충남대학교대학원 범종일 서미자 윤영남 유용만, 2011, 딱정벌레목에서분리한 Bacillus thuringiensis sub 네. CAB530 균주의살충활성및유전학적특성, 2011 년한국농약과학회정기총회및춘계학술발표회, p.120 최홍서, 2003, Bacillus thuringiensis(bt) 에의해생산되는 δ-endotoxin 에관한연구, 한남대학교대학원 Deng C, Peng Q, Song F, Lereculus D, 2014 Jul 23, Regulation of cry gene expression in Bacillus thuringiensis. Toxin(Basel)

40 Reference Doi Y Teranaka M Yora K Asuyama H, 1967, Mycoplasma or PLT-group-like organisms found in the phloem elements of plants infected with mulberry dwarf, potato witches' broom, aster yellows or paulownia witches' broom, Annals of the Phytopathological Society of Japan 33 (4), pp Evelyn Strauss, 2009, Phytoplasma Research Begins to Bloom, Science Vol. 325 No.5939, pp George N. Agrios, 2006, 식물병리학, 월드사이언스, pp Kyoko Sugawara, Youhei Honma, Ken Komatsu, Misako Himeno, Kenro Oshima, Shigetou Namba, 2003, The Alteration of Plant Morphology by Small Peptides Released from the Proteolytic Processing of the Bacterial Peptide TENGu. Marcone C Gibb KS Streten C Schneider B, 2004, Candidatus Phytoplasma spartii, 'Candidatus Phytoplasma rhamni' and 'Candidatus Phytoplasma allocasuarinae', respectively associated with spartium witches'-broom, buckthorn witches'-broom and allocasuarina yellows diseases, International Journal of Systematic and Evolutionary Microbiology, pp Proc Natl Acad Sci U S A Nov 29; 108(48): E1254 E1263. Published online 2011 Nov 7.

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