Some of the proteins we will study. Biophysics 10 #2 your proteins. Bioenergetic proteins in membranes !"#$%&$'()"*+,+

Transcription

1 Biophysics 10 #2 your proteins Electron & proton transfer proteins ATPases Ion and proton channel proteins Some of the proteins we will study Electron transfer proteins non-protein pieces that gain or loose electrons electrons move from negative to positive potential - energy is stored in a proton gradient Physics: electron tunneling 1 2!"#$%&$'()"*+,+ Bioenergetic proteins in membranes Saraste, M. (1999) Oxidative phosphorylation at the fin de siecle, Science 283, 777/'%8"/'46/'"&/9$:28$$;-&14<2=+>&1+%144+?481)#-+1#+*4 -#*./0%%%/'1#23(1%14423&$#$-5'2%&4$)34*/&#64 3 Saraste, M. (1999) Oxidative phosphorylation at the fin de siecle, Science 283,

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

3 G&$#$-5'#&1V%+^>+%&*"' >&1+!"#$%&$'()"*4+141%#)$'+#)*'-<1)+%&*"' TaI D 1= 1= 1= 1= 1= 1= IY IY IY 777/L_#)/%*24*8%*)31'V1)21'92&$61`<)*61-/&#6 IY IY IY &: Newmeyer, D. D., and Ferguson-Miller, S. (2003) Mitochondria: releasing power for life and unleashing the machineries of death, Cell 112, cudi>+gtki^k+>i^+^c^@>ejmk+tgiucc K6*44+9)$L3+$<+)1($H+%$<*%#$)- ^41%#)$'+b$7+($7'&"44+<)$6+%5#+%+#$+J D + T8"_L"'$'1?>G G)$#$'-+b$7+L3&"44+#$+$L#-"(1+%

4 e1($h+%&16"-#)5 A+141%#)$' Y\ YD 1 = J f f J D+141%#)$'-+Y+D+3)$#$'- JI = JI = 13 DF+41O1)-+"'+#&1+%$(1g+#&1+-1_L1'%1+(1#1)6"'1-+#&1+<$4(+*'(+<L'%V$' 14 GKUU G&$#$-5-#16+UU+%$<*%#$)- &O3P22777/8"$/"%/*%/L;2)1-1*)%&28*)81)23-D6"%)$-"#129*441)5/&#64 RU;"31("* 15 16

5 overview C.C. Moser et al. / Biochimica et Biophysica Acta 1757 (2006) Complex 1: Nadh-UQ oxidoreductase Complex I1: Succinate Dehydrogenase Complex I1I: Cytochrome bc1 Dehydrogenase C.C. Moser et al. / Biochimica et Biophysica Acta 1757 (2006) Complex IV: Cytochrome c oxidase Table 1 Estimates of single-electron redox center potentials in respiratory Mitochondrial complex Redox center Em7 I N1a 0.38 N1b 0.25 N N N N N6a 0.25 N6b 0.25 II [2Fe2S] 0.00 [4Fe4S] [3Fe4S] 0.06 heme III c FeS 280 bl 50 bh 80 IV Cu A.24 a 0.26 CuB 0.28 (0.32) Tyr E+)1(+3&$#$'-+XBFF+'6hA/Z1[]+#$+#*;1+E+1 W +<)$6+I D J+*'(+3L#+#&16+$'+a+#$+6*;1+J D +*'(+ai D DI D JYDa+fi+J D +Y+DaI D ++\/\+1[+]h<=>%!?&2!$.] D+a2aI D =F/Fj1[!'-,%./(" A/ZkE1[ $%&&'(&" )('*+," cudi> F/ZD1[ J D +2I D J!" #" C.C. Moser et al. / Biochimica et Biophysica Acta 1757 (2006)

6 Some of the proteins we will study Some of the proteins we will study ATPase - making usable biological energy from the proton gradient Physics - molecular motors Proton or ion transfers in (bacterio)rhodopsins and in ion or proton channels Physics - moving charges out of water; the energy stored in a membrane gradient looking at channels br: FAWOqpg&feature=related lipids: v=7k2kafrsz4q&feature=related aquoporin: aquaporins/ Where is the physics? Forces that hold proteins together in the proper shape. Energies to move groups from water to protein to lipid. The storage of energy in transmembrane Δψ (electrical and ion gradient). Transfer of electrons happen by electron tunneling (no QM no mitochondria) 23 24