Appendix A SPR Sensorgrams for Interactions of 3 Serum Proteins with Gold Nanoparticles

Transcription

1 Appendix A SPR Sensorgrams for Interactions of 3 Serum Proteins with Gold Nanoparticles Further from Sect (Figs. A.1, A.2 and A.3). Springer International Publishing Switzerland 2017 A. Patra, Quantifying Interactions of Biomolecules with Inorganic Surfaces, Springer Theses, DOI /

2 96 Appendix A: SPR Sensorgrams for Interactions Fig. A.1 SPR Sensorgram for ApoA1 interacting with AuNPs. Different colours represent different concentrations. Concentrations shown in legend. 3 sets of data have been shown

3 Appendix A: SPR Sensorgrams for Interactions 97 Fig. A.2 SPR Sensorgram for HSA interacting with AuNPs. Different colours represent different concentrations. Concentrations shown in legend. 3 sets of data have been shown

4 98 Appendix A: SPR Sensorgrams for Interactions Fig. A.3 SPR Sensorgram for IgG interacting with AuNPs. Different colours represent different concentrations. Concentrations shown in legend. 3 sets of data have been shown

5 Appendix B SPR Sensorgrams for Interactions of PEG with Chemically Active Chip Surface See Fig. A.4. Fig. A.4 Control studies to show no interaction of PEG with the chemically active linker surface. Lanes 1, 2, 3 and 4 have gold nanoparticles and PEG is seen to adsorb onto the captured nanoparticles. When there are no captured gold nanoparticles, PEG doesn t adsorb at all onto the chip surface Springer International Publishing Switzerland 2017 A. Patra, Quantifying Interactions of Biomolecules with Inorganic Surfaces, Springer Theses, DOI /

6 Curriculum Vitae Dr. Abhijeet Patra NUS Nanoscience and Nanotechnology Institute #11-01, T-Lab 5A Engineering Drive 1 Singapore abhijeetpatra88@gmail.com Professional Profile A researcher working in the area of bio-nanotechnology with expertise in studying interactions of biomolecules with organic/inorganic surfaces or assemblies and tailoring cell/bacterial growth by using metal oxide thin films. Education and Qualifications Ph.D. Bachelor of Technology NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore (Aug 2011 Nov 2015) Electrical and Electronics Engineering, National Institute of Technology, Tiruchirappalli, India ( ) Graduate Projects: Development of new protocols/technologies to study interaction of biomolecules with organic/inorganic surfaces or assemblies for the purposes of therapeutics delivery and purification of selected groups of molecules. Development of new protocols/technologies for rapidly screening materials for cell/bacterial growth. Publications: 1. Patra, Abhijeet, Tao Ding, Gokce Engudar, Yi Wang, Michal Marcin Dykas, Bo Liedberg, James Chen Yong Kah, Thirumalai Venkatesan, and Chester Lee Drum. Component Specific Analysis of Plasma Protein Corona Formation on Gold Nanoparticles Using Multiplexed Surface Plasmon Resonance. Small (2015). Springer International Publishing Switzerland 2017 A. Patra, Quantifying Interactions of Biomolecules with Inorganic Surfaces, Springer Theses, DOI /

7 102 Curriculum Vitae 2. Sarkar, T.P., K. Gopinadhan, M. Motapothula, S. Saha, Z. Huang, S. Dhar, A. Patra et al. Unexpected observation of spatially separated Kondo scattering and ferromagnetism in Ta alloyed anatase TiO 2 thin films. Scientific reports 5 (2015). 3. Li, J., Yoong, S.L., Goh, W.J., Czarny, B., Yang, Z., Poddar, K., Dykas, M.M., Patra, A., Venkatesan, T., Panczyk, T. and Lee, C., In vitro controlled release of cisplatin from gold-carbon nanobottles via cleavable linkages. International journal of nanomedicine, 10, p Positions of responsibility Oct 2014 Coordinator, NanoSpark Hosting, managing and coordinating events for students, faculty members and entrepreneurs. Events include talks, interactive sessions, networking dinners. Jan 2014 May 2014 Teaching assistant, Bioengineering, NUS Handled tutorials and laboratory sessions for an undergraduate module on BioSignal Processing, taught by Asst. Prof. James Kah. Aug 2010 March 2011 Head, Events, Pragyan Selecting and managing events to be conducted at Pragyan, a national level technical festival in India with a budget of S$210,000. Awards NGS scholarship to pursue graduate studies at National University of Singapore, Aug 2011 to Aug 2015 NUS Enterprise Practicuum Grant 7.5K SGD for the development of a prototype Best Oral Presentation, Life Opportunities Research Symposium 2014, NUS Runners up at ASEAN Science Slam 2013 organised by EURAXESS, 2013 Indian National Academy of Engineers Fellowship, May to July 2010.