Drosophila Mapping Homework
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1 Drosophila Mapping Homework In Drosophila, the genes stubble bristle (S), vestigial wings (V) and black body (B) are autosomally linked. The mutant alleles are all recessive to wild type. Determine the map in each of the problems and calculate the coefficient of coincidence and interference. Problem 1 Problem 2 Problem 3 Phenotype Number Phenotype Number Phenotype Number B s v 570 B s v 1 B s v 84 b S V 559 b S V 3 b S V 90 B S v 1 B S v 51 B S v 1350 b s V 2 b s V 58 b s V 1356 B s V 37 B s V 2104 B s V 54 b S v 41 b S v 2089 b S v 60 b s v 18 b s v 92 b s v 2 B S V 22 B S V 102 B S V Problem 4 In Drosophila the genes A B and D are autosomally linked according to the map below. In that the coefficient of coincidence is A B D 5.8 cm 2.6 cm Problem 5 In Drosophila the genes A B and D are autosomally linked according to the map below. In that the coefficient of coincidence is D A B 4.6 cm 3.8 cm Problem 6 In Drosophila the genes A B and D are autosomally linked according to the map below. In that the coefficient of coincidence is 0.92 A D B 4.5 cm 2.5 cm
2 Questions Use this information for Problems 1 3 Possible P1 parents for problems 1-3 cross: a. stubble black vestigial x wild type b. stubble black x vestigial c. stubble vestigial x black d vestigial black x stubble Possible non-recombinant and recombinant reciprocal phenotype pairs for problems 1-3: a b c d BSv BsV bsv bsv BSV bsv bsv Bsv Possible middle gene for problems 1-3: a. gene B b. gene S c. gene V Problem 1 (use these values for problem 1) a b d e What were the P1 parents of this cross? 2 Which phenotype set represents the non-recombinants? 3 Which phenotype set represents a double crossover? 4 Which gene is in the middle? 5 What is the distance between B and S? 6 What is the distance between S and V? 7 What is the distance between B and V? 8 What is the coefficient of coincidence? 9 What is the interference? 10 Is this positive interference (a) or negative interference (b)?
3 Problem 2 (use these values for problem 2) a b c d e What were the P1 parents of this cross? 12 Which phenotype set represents the non-recombinants? 13 Which phenotype set represents a double crossover? 14 Which gene is in the middle? 15 What is the distance between B and S? 16 What is the distance between S and V? 17 What is the distance between B and V? 18 What is the coefficient of coincidence? 19 What is the interference? 20 Is this positive interference (a) or negative interference (b)? Problem 3 (use these values for problem 3) a b c d e What were the P1 parents of this cross? 22 Which phenotype set represents the non-recombinants? 23 Which phenotype set represents a double crossover? 24 Which gene is in the middle? 25 What is the distance between B and S? 26 What is the distance between S and V? 27 What is the distance between B and V?
4 28 What is the coefficient of coincidence? 29 What is the interference? 30 Is this positive interference (a) or negative interference (b)? Use this information for Problems 4-6 Possible non-recombinant and recombinant reciprocal phenotype pairs for problems 4-6: a b c d ABd AbD abd abd ABD abd abd Abd Problem 4 31 Which phenotype set represents non-recombinants? 32 What is the expected number for this set? a 1380 d 1372 b 1376 e 1368 c Which phenotype set represents double recombinants? 34 What is the expected number for this set? 35 Which phenotype set represents a single crossover between A and B? 36 What is the expected number for this set? a 83 d 89 b 87 e 85 c Which phenotype set represents a single crossover between B and D? 38 What is the expected number for this set? a 41 d 35 b 39 e 33 7 Problem 5 39 Which phenotype set represents non-recombinants? 40 What is the expected number for this set? a 1380 d 1372 b 1376 e 1368 c Which phenotype set represents double recombinants?
5 42 What is the expected number for this set? 43 Which phenotype set represents a single crossover between D and A? 44 What is the expected number for this set? a 63 d 69 b 65 e 67 c Which phenotype set represents a single crossover between A and B? 46 What is the expected number for this set? a 61 d 55 b 59 e 53 c 57 Problem 6 47 Which phenotype set represents non-recombinants? 48 What is the expected number for this set? a 1390 d 1396 b 1388 e 1394 c Which phenotype set represents double recombinants? 50 What is the expected number for this set? 51 Which phenotype set represents a single crossover between D and A? 52 What is the expected number for this set? a 66 d 62 b 64 e 60 c Which phenotype set represents a single crossover between D and B? 54 What is the expected number for this set? a 38 d 36 b 32 e 34 0
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