Confounding and Fractional Replication in Factorial Design
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1 ISSN -580 (Paper) ISSN 5-05 (Online) Vol.6, No.3, 016 onfounding and Fractional Replication in Factorial esign Layla. hmed epartment of Mathematics, ollege of Education, University of Garmian, Kurdistan Region Iraq bstract In factorial experiments when the number of factors or the levels of factors are increased the number of treatment combinations increased rapidly. lso, it becomes difficult to maintain the homogeneity between experimental units. To overcome the decrease of the experimental units, we need to decrease the number of those treatments by using a confounded design (complete and partial) and fractional replication design. factorial experiment for in randomized complete block design with four blocks has been applied, for the aim of comparison among factorial randomized complete block design, confounded designs and fractional replication design in applied factorial experiments. Key Words: Factorial Experiment, omplete onfounding, Partial onfounding, Half fractional Replication The im of Study The study aims to comparison among the results of factorial experiment conducted in randomized complete block design, complete confounding, partial confounding and half fractional replication, using mean squares error to differentiate the results of this study. 1.. Introduction In factorial experiments when the number of factors or number of levels of the factors increase, the number of treatment combinations increase very rapidly and it is not possible to accommodate all these treatment combinations in a single homogeneous block. For example, a 5 factorial would have 3 treatment combinations and blocks of 3 plots are quite big to ensure homogeneity within them. new technique is there for necessary for designing experiments with a large number of treatments. In order to keep the advantages of the factorial experimental error to a minimum, a device known as confounding or fractional factorial is adopted. Fisher (196) first suggested the confounded design. Fisher and Wishart (1930) gave the explanation of the numerical procedure of the analysis of randomized block and Latin square experiments; they also gave an example of a confounded experiment [6]. The use of experiments in factorial replication was proposed in (195) by Finney. He outlined methods of construction for n and 3 factorials and described a half- replicate of a, agricultural field experiment that had been conducted in 19 [3] Factorial Experiments In a factorial experiment the treatments are combinations of two or more levels of two or more factors. factor is a classification or categorical variable which can take one or more values called levels []. 17
2 ISSN -580 (Paper) ISSN 5-05 (Online) Vol.6, No.3, 016 Factorial experiments provide an opportunity to study not only the individual effects of each factor but also their interactions. They have the further advantage of economizing on experimental resources [6]. The mathematical model for factorial R is [7]: i = 1,,.., a y ijk = μ + α i + β j + (αβ) ij + ρ k + ε ijk { j = 1,,.., b k = 1,,., r (1) Where μ is the overall mean, α i is the effect of the i th level of factor, β j is the effect of the j th level of factor, (αβ) ij is the effect of the interaction between the i th level of factor and j th level of factor,, ρ k is the effect of the k th block, and ε ijk is the random error associated with the k th replication in cell (ij). In the two factors fixed effects model, we are interested in the hypotheses: main effect: H 0 : α 1 = = α a = 0 } () H 1 : at least one ofα i 0 main effect: H 0 : β 1 = = β b = 0 } (3) H 1 : at least one ofβ j 0 interaction effect: H 0 : (αβ) 11 = = (αβ) ij = 0 } () H 1 : at least one of(αβ) ij 0 Table1: NOV for the factorial R (S.O.V.) (d.f.) (S.S.) (M.S.) (F.al) locks r-1 Y.. k ab ( Y...) abr SSbl. r 1 F cal Mbl MSE a-1 Y i.. rb ( Y...) abr SS a 1 MS F cal MSE b-1 Y. j. ra ( Y...) abr SS b 1 MS F cal MSE (a-1)(b-1) Y ij.. r ( Y...) abr SS SS SS( ) ( a 1)( b 1) MS( ) F cal MSE Error (r-1)(ab-1) SST SSbl. SS SS SS() SSE ( r 1)( ab 1) Total abr-1 SST = Y ijk Y abr 18
3 ISSN -580 (Paper) ISSN 5-05 (Online) Vol.6, No.3, onfounding onfounding is a technique for designing experiments with a large number of treatments in factorial experiments. The treatment combinations are divided into as many groups as the number of blocks per replication. The different groups of treatments are allocated to the blocks. The grouping of treatments combinations must be done in such a way that only the unimportant effects are confused with the block effects and other import anted effects could be evolved compare significantly[]. There are two types of confounding [], [3]: complete confounding and partial confounding. If the same effect confounded in all the other replications, then the interaction is said to be completely confounded. nd all the information on confounded interactions are lost. When an interaction is confounded in one replicate and not in another, the experiment is said to be partially confounded. The confounded interactions can be recovered from these replications in which they are not confounded. The table () of positives and negatives signs for the design. The signs in the columns of this table can be used to estimate the factor effects. Table: Table of positive and negative signs for the Trea. om a b ab c ac bc abc d ad bd abd cd acd bcd abcd Fractional Factorial esigns s the number of factors in a k factorial design increases, the number of trials required for a full replicate of the design rapidly outgrows the resources available for many experiments. In such cases, one cannot perform a full replicate of the design and a fractional factorial design has to be run [8]. Such an experiment contains one- half fraction of a experiment and is called 1 factorial experiment. Similarly, 1 fraction of 3 factorial experiment requires only 8 runs and contains 1 fraction of factorial experiment and called as factorial experiment. In general, contains 1 fraction of a p k factorial experiment 19
4 ISSN -580 (Paper) ISSN 5-05 (Online) Vol.6, No.3, 016 requires only k p runs and is denoted as k p factorial experiment [9]. 1 fractional can be generated from any interaction, but using the highest - order interaction is the standard. The interaction used to generate 1 fraction is called the generator of the fractional factorial design. When there are factors, use as the generator of the 1 design. ased on the signs (positive or negative) as shown in table (), attached to the treatments in this expression, two groups of treatments can be formed out of the complete factorial set. Retaining only one set with either negative or positive signs, we get a half fractional of the factorial experiment. The two sets of treatments are shown below. Treatments with negative signs a b c abc d abd acd bcd Treatments with positive signs 1 ab ac bc ad bd cd abcd The alias structure for this design is found by using the defining relation I =. Multiplying any effect by the defining relation yields the aliases for that effect. The alias of is =. I =. = = liases are two factorial effects that are represented by the same comparisons. Thus and are aliases. Similarly, we have other aliases: =, =, = =, =, = The treatment combinations in the 1 design yields four degrees of freedom associated with the main effects. From the upper half of table, we obtain the estimates of the main effects as linear combinations of the observations, = 1 [ad + ab + ac + abcd 1 bd cd bc] (5) = 1 [bd + ab + bc + abcd 1 ad cd ac] (6) = 1 [cd + ac + bc + abcd 1 ad bd ab] (7) = 1 [ad + bd + cd + abcd 1 ab ac bc] (8) = 1 [1 + ab + cd + abcd ad bd ac bc] (9) 0
5 ISSN -580 (Paper) ISSN 5-05 (Online) Vol.6, No.3, 016 = 1 [1 + bd + ac + abcd ad ab cd bc] (10) = 1 [1 + ad + bc + abcd bd ad cd ac] (11). pplications This section tackles the practical application of the factorial experiment for in randomized complete block design with four blocks given in ochran and ox (1957) has been applied, for the aim of comparison among factorial randomized complete block design, confounded designs and fractional replication design. The minitab 16 is used. Then the resulting data is as follows: Treatment combination 1 a b ab c ac bc abc d ad bd abd cd acd bcd abcd Table 3: ata experiment Rep. 1 Rep. Rep. 3 Rep. Total Total
6 Term Mathematical Theory and Modeling ISSN -580 (Paper) ISSN 5-05 (Online) Vol.6, No.3, Full Factorial Table : NOV for full factorial R S.O.V.F SS MS F P- value Replication * * * * * * * Error Total *significant at level (0.05) Figure 1: Pareto plot for full factorial R Pareto hart of the Standardized Effects (response is y, lpha = 0.05).01 F actor Name 0 6 Standardized Effect 8 10 In the analysis, the results show those main effects,, and and the two factor interactions, and three factor interaction are significant and the interactions,,,,,,, are non significant at the level of significant (α=0.05). nd the Pareto plot looks at the effects and orders them from largest to smallest as shown in figure omplete onfounding The experiment with four factors,,, and, each at two levels. There are only 16 treatment combinations.
7 ISSN -580 (Paper) ISSN 5-05 (Online) Vol.6, No.3, 016 a. Suppose that each replicate in experiment is divided in to two blocks of eight units each, such that one block contains all treatment combinations that have on positive signs, while the other contains all negative signs. The interaction of highest order is the interaction. This interaction is estimated from the comparison. The plan would be as follows: Table 5: Plan for factorial, blocks of 8 units, with confounded Replicate 1 Replicate Replicate 3 Replicate (1) 3 (a) 7 (1) 3 (a) 1 (1) 7 (a) 8 (1) 19 (a) 5 (ab) 61 (b) 6 (ab) 76 (b) 36 (ab) 56 (b) (ab) 6 (b) 18 (ac) 51 (c) 9 (ac) 3 (c) 39 (ac) 0 (c) 7 (ac) 8 (c) 8 (bc) 36 (abc)76 (bc) 31 (abc) 65 (bc) 3 (abc)70 (bc) 30 (abc) 63 (ad) 63 (d) 35 (ad) 1 (d) (ad) 60 (d)56 (ad) 53 (d) 35 (bd) 80 (abd)100 (bd) 68 (abd) 68 (bd) 75 (abd) 87 (bd) 67 (abd) 66 (cd) 0 (acd)6 (cd) (acd) 39 (cd) 53 (acd) 75 (cd) 36 (acd) 7 (abcd) (bcd)105 (abcd) (bcd) 99 (abcd) 89 (bcd) 7 (abcd)101 (bcd) orect Factor(. F) = SSTotal = F = SSRepl. = 93.3 SSlock = (53) +(8) + +(00). F = SS(lock/Rep) = SSlock SSRep. = The sums of squares for the main effects and interactions are calculated using the factorial effect totals which can be obtained by the Yates method as shown in table (6). 3
8 ISSN -580 (Paper) ISSN 5-05 (Online) Vol.6, No.3, 016 Table 6: Yates method for effect totals Treat. comb. Total Treatments Sum and different of pairs I II III IV SS= [IV] 1 a b ab c ac bc abc d ad bd abd cd acd bcd abcd Table 7: NOV with onfounded S.O.V.F SS MS F P- value locks r-1= lock/repl. r = * * * * * * * Error Total *significant at level (0.05) In the analysis, the results show those main effects,, and and the two factor interactions, and three factor interaction are significant and the interactions,,,,,,, are non significant at the level of significant (α=0.05). While the mean squares error is equal to (9.8) greater than the result of the analysis in the table () and that the mean squares error is equal to (90.5). b. Each replicate in experiment is divided in to four blocks of four units each, the interactions of, and completely confounded, = = There will be k p = = blocks per replicate.
9 ISSN -580 (Paper) ISSN 5-05 (Online) Vol.6, No.3, 016 Let X 1, X, X 3, and X denoted the levels (0 or 1) of each of the factors,, and. Solving the following equations would result in different blocks of the design: For interaction : X 1 + X + X 3 = 0,1 For interaction : X + X 3 + X = 0,1 Treatment combinations satisfying the following solutions of above equations will generate the required blocks: (0,0), (0,1), (1,0), (1,1). The solution (0,0) will give the key block(a key block is one that contains one of the treatment combination of factors, each at lower level)[].similarly we can write the other blocks by taking the solutions of above equations as (0,1), (1,0) and (1,1). In this case that each replicate in experiments divided into blocks of units each, with replicates the plan would be as follows: Table 8: Plan for factorial, blocks of units, with, and confounded Replicate 1 Replicate (b)6 (a)7 (d)35 (bc)36 (c ) 39 (bd)68 (ab)76 (acd)39 (c ) 9 (bd)80 (ab)61 (abd)100 (b) 36 (a)1 (d) (bc)31 (ad)63 (cd)0 (ac)51 (acd)6 (ad)1 (abc)65 (ac)3 (abd)68 (abcd)90 (abc)76 (bcd)105 (1)3 (abcd)8 (cd) (bcd)99 (1) Replicate 3 Replicate (ad)60 (bd)75 (ab)56 (abd)87 abcd)101 (abc)63 (ac)8 (1)19 (abcd)89 (abc)70 (ac)0 (1)7 (b)18 (bd)67 (bcd)73 (acd)7 (c )7 (a)8 (acd)7 (bc)3 (ad)53 (cd)36 (d)35 (abd)66 (b) (cd)53 (d)56 (acd)75 (c) 8 (a)5 (ab)6 (bc) SSTotal = F = SSRepl. = 93.3 SSlock = (08) +(3) + +(187). F = SS(lock/Rep) = SSlock SSRep. =
10 ISSN -580 (Paper) ISSN 5-05 (Online) Vol.6, No.3, 016 The sums of squares for the main effects and interactions are calculated using the factorial effect totals which can be obtained by the Yates method as shown in table (6), and the analysis of variance as shown in table (9). Table 9: NOV with, and onfounded S.O.V.F SS MS F P- value locks r-1= lock/rep r (b-1)= * * * * * * Error Total *significant at level (0.05) In the analysis, the results show those main effects,,, and and the two factor interactions and three factor interaction are significant and the interactions,,,,, are non significant at the level of significant (α=0.05), nd the mean squares error is equal to (101.1)....Partial onfounding onsider again experiment with each replicate divided into two blocks of 8 units each. It is not necessary to confound the same interaction in all the replicates and several factorial effects may be confounded in one single experiment. The following plan confounds the interaction,, and in replicates 1,, 3 and respectively. 6
11 ISSN -580 (Paper) ISSN 5-05 (Online) Vol.6, No.3, 016 Table 10: Plan for factorial, blocks of 8 units, with,, and partially confounded Replicate 1 Replicate Replicate 3 Replicate onfound onfound onfound onfound (1) 3 (a) 7 (a) 1 (1) 3 (a) 8 (1) 7 (b) 18 (1) 19 (ab) 61 (b) 6 (b) 36 (ab) 76 (ab) 56 (b) (ab) 6 (a)5 (ac) 51 (c) 9 ( c) 39 (ac) 3 ( c) 7 (ac) 0 (c ) 8 (bc) 30 (bc) 36 (abc)76 (abc) 65 (bc) 31 (bc) 3 (abc) 70 (ac) 8 (abc) 63 (ad) 63 (d) 35 (ad) 1 (d) (d) 56 (ad) 60 (d) 35 (bd) 53 (bd) 80 (abd)100 (bd) 68 (abd) 68 (bd) 75 (abd) 87 (ad) 67 (abd) 66 (cd) 0 (acd)6 (cd) (acd) 39 (acd) 75 (cd) 53 (bcd) 73 (cd) 36 (abcd) 90 (bcd)105 (abcd) 8 (bcd) 99 ( abcd)89 (bcd) 7 (abcd)101 (acd) The sums of squares for blocks and for the not confounded effects are found in the usual way (see table Yates method). SSRepl. = 93.3 SSlock = (53) +..+(38). F = SS(lock/Rep) = SSlock SSRep. = The sum of squares for is calculated from replicates (, 3, ), similarly it is possible to recover information on the other confounded interactions (from 1, 3, ), (from 1,, ) and (1,, 3) as shown in table (11). The sum of squares for partially confounded are calculated as follows: SS = 1 + ab + ac + bc + ad + bd + cd + abcd) (r 1) [(I (a + b + c + abc + d + abd + acd + bcd) ] = 1 8 [ 1] = SS = 1 + b + c + abc + ad + bd + cd + abcd) (r 1) [(a (I + ab + ac + bc + d + abd + acd + bcd) ] = 1 8 [ 30] = SS = 1 + ab + c + bc + d + bd + acd + abcd) (r 1) [(a (I + b + ac + abc + ad + abd + cd + bcd) ] = 1 8 [3] = SS = 1 + ab + c + ac + d + ad + bcd + abcd) (r 1) [(b (I + a + bc + abc + bd + abd + cd + acd) ] = 1 8 [ 6] = (1) (13) (1). (15) 7
12 ISSN -580 (Paper) ISSN 5-05 (Online) Vol.6, No.3, 016 Table 11: NOV for partial confounded S.O.V.F SS MS F P- value Replications r-1= lock/repl. r = * * * * * () * () () () Error Total *significant at level (0.05) In the analysis, the results show those main effects,, and and the two factor interactions, and three factor interaction are significant and main effect and the interactions,,,,,,, are non significant at the level of significant (α=0.05). While the mean squares error is equal to (9.9) less than the results of the analysis for complete confounding with blocks and complete confounding with blocks and that the mean squares errors are equal to (9.8) and (101.1) respectively..3. Fractional Replication There are factors, use as the generator of the 1 design. ased on the signs (positive or negative) as shown in table (), attached to the treatments in this expression, two groups of treatments can be formed out of the complete factorial set. Retaining only one set with either negative or positive signs, we get a half fractional of the factorial experiments. The alias structure for this design is found by using the defining relation I =. Multiplying any effect by the defining relation yields the aliases for that effect. The alias of is =. I =. = = liases are two factorial effects that are represented by the same comparisons. Thus and are aliases. Similarly, we have other aliases: =, =, =. F = (G.Total) rt. F = (17) (8) = SSTotal = F = SSRepl. =
13 Term Mathematical Theory and Modeling ISSN -580 (Paper) ISSN 5-05 (Online) Vol.6, No.3, 016 For the four factors tested, a 1 fractional factorial design is a Resolution IV design. The resolution of the design is based on the number of the letters in the generator. The main effects are aliased with three way interactions and the two way interactions are aliased with each other [1]. Therefore, we cannot determine from this type of design which of the two way interactions are important because they are confounded or aliased with each other. The sums of squares for the main effects and interactions are calculated as shown in table (1). Table 1: NOV for fractional replication S.O.V.F SS MS F P- value Replications r-1= * * * * * Error Total *significant at level (0.05) Figure : Pareto plot for fractional replication Pareto hart of the Standardized Effects (response is y, lpha = 0.05).06 F actor Name Standardized Effect
14 Percent Mathematical Theory and Modeling ISSN -580 (Paper) ISSN 5-05 (Online) Vol.6, No.3, 016 Figure 3: Normal probability plot of the effects Normal Plot of the Standardized Effects (response is y, lpha = 0.05) Effect Type Not Significant Significant F actor Name Standardized Effect 6 8 The result in table (1) shows those main effects,, and and the two factor interactions, are significant and main effect and the interactions are non significant at the level of significant (α=0.05), and the normal probability plot is very useful in assessing the significance of effects from a fractional factorial design, particularly when many effects are to be estimated. Figure (3) presents the normal probability plot of the effects. Notice that the,,, and effects stand out clearly in this graph. onclusions 1. The result of analysis of variance for factorial randomized complete block design showed that the mean squares error is equal to (90.5).. When each replicate in experiment contains two blocks of eight units each and the interaction of completely confounded, the mean squares error is equal to (9.8). While, each replicate in experiment contains four blocks of four units each, and the interactions are completely confounded, the mean squares error is equal to (101.1) greater than the result of the analysis in the full factorial. 3. Partially confounding has been most efficient, the value of mean squares error is (9.9) less than the result of the analysis in completely confounded.. The result of analysis of variance showed that the fractional factorial design is the highest accuracy in estimating the effects and was the best in saving time and cost. References [1] abiak, I., rzuska and Perkoski, J., 000, Fractional Factorial esigns of Screening Experiments on ryopreservation of Fish Sperm, quaculture Research, 31, pp [] lewer,. G. and Scarisbrick,. H., 001, Practical Statistics and Experimental esign for Plant and rop Science, John Wiley and Sons, Ltd, New York. [3] ochran, W. G. and ox, G. M., 1957, Experimental esigns, Second Edition, John Wiley and Sons, Inc, New York. [] Jaisankar, R. and Pachamuthu, M., 01, Methods for Identification of onfounded Effects in Factorial Experiments, Int. J. of Mathematical Sciences and pplications, Vol., No., pp
15 ISSN -580 (Paper) ISSN 5-05 (Online) Vol.6, No.3, 016 [5] Jalil, M.., 01, General onstruction Method of Simultaneous onfounding in p n - Factorial Experiments, haka Univ. J. Sci. Vol. 60, No., pp [6] Jerome,. R., 19, esign and Statistical nalysis of Some onfounded Factorial Experiments Research ulletin 333. [7] Montgomery,.. and Runger, G.., 00, pplied Statistics and Probability for Engineers, 3 rd Edition, John Wiley and Sons, Inc, US. [8] Ranjan, P., 007, Factorial and Fractional Factorial esigns with Randomization Restrictions a Projective Geometric pproach, Ph.sc thesis, Indian Statistical Institute. [9] Shalabh, H. T., 009, Statistical nalysis of esign Experiments, 3 rd, Springer New York ordrecht Heidelberg London. 31
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