Photosynthetic picoeukaryote assemblages in the South China Sea from the Pearl River estuary to the SEATS station

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1 The following supplement accompanies the article Photosynthetic picoeukaryote assemblages in the South China Sea from the Pearl River estuary to the SEATS station Wenxue Wu, Bangqin Huang*, Chao Zhong Key Laboratory of Coastal and Wetland Ecosystems, Ministry of Education / State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen , PR China *Corresponding author: bqhuang@xmu.edu.cn Aquatic Microbial Ecology 71: (2014) Supplement. List of sequence assignments in this study (Table S1) and salinity distribution at the surface and 75 m depth in the northern South China Sea during the cruise (Fig. S1) Table S1. List of all the operational taxonomic units (OTUs, grouping sequences with similarities >98%) recovered in this study. For each OTU, a representative sequence is provided with its taxonomic affiliation, its GenBank closest relative with accession number (12 September 2013 database), the sequence percent similarity, BLAST score, Query/Subject ratio, and OTU frequency from each library. MAST = novel marine stramenopiles OTU OTU frequency Clone Taxonomy Closest relative GenBank Accession Similarity (%) BLAST score Query/ Subject CC02A740 CC02A105 CC02A175 CC02SE05 CC02SE75 Alveolata CC02A Alveolates group I C5 FN / CC02A FR / F07 CC02A Uncultured marine alveolate EU / CC02SE F1 FN / CC02A Uncultured marine picoeukaryote clone 1802G10 FR /

2 CC02A AY / SCM37C10 CC02A Uncultured phytoplankton clone JQ / Q1-17 CC02A SCM27C44 AY / CC02A OLI DQ / CC02A SCM37C10 AY / CC02SE HQ / CC02SE CC02A CC02A CC02A CC02A CC02A CC02A CC02A CC02A CC02A CC02SE CC02SE CC02SE CC02A CC02A Alveolates group II SCM37C36 RS.12f.10m SCM37C7 SSRPC57 NAMAKO-23 RS.12f.10m.264 Amoebophrya sp. ex Karlodinium micrum SSRPC41 SSRPC54 SSRPC86 RS.12f.10m.0035 RS.12f.10m RS.12f.10m.316 SSRPC86 Uncultured marine dinoflagellate clone Ma131_1A49 AY / KC / AY / EF / AB / KC / AF / EF / EF / EF / KC / KC / KC / EF / FJ /

3 CC02A JN / E_NY_2E08 CC02A JQ / MAL106_6_A1 CC02A EF / SSRPC27 CC02A KC / RS.12f.10m.163 CC02A KC / RS.12f.10m.243 CC02A EF / SSRPB41 CC02A KC / RS.12f.10m.004 CC02SE RS.12f.10m.41 KC / CC02A KC / CC02A RS.12f.10m.316 KC / CC02A KC / RS.12f.10m.163 CC02SE KC / RS.12f.10m.0032 CC02SE AY / clone UEPAC05Ap2 CC02SE AY / clone UEPACEp3 CC02A JN / E_MO_3E06_OTU24 CC02SE Alveolates AY / group III SCM15C23 CC02A Alveolates Uncultured syndiniales clone JF / group V 1528 CC02SE Uncultured syndiniales clone JF / CC02A Ciliophora EF / clone SA2_1B7 CC02A Pelagostrobilidium sp. PCC-2012 JQ / CC02A clone UEPACAIp5 AY / CC02A clone NW DQ /

4 CC02A AY / SCM16C16 CC02SE Dinophyceae AY / SCM15C21 CC02SE DQ / clone M3_18B06 CC02A AY / SCM37C24 CC02A JN / E_MO_3F01_OTU3 CC02A AY / SCM15C21 CC02A AY / SCM28C40 CC02SE HM / clone CNCIII05_240 CC02A AY / SCM38C48 CC02A Unknown Uncultured dinoflagellate clone EU / group PROSOPE.EM-110m.155 Chlorophyta CC02A Prasinophyceae Uncultured prasinophyte clone RA AY / CC02A Prasinophyte sp. RCC 287 AY / CC02A Eukaryote clone OLI11305 AJ / CC02SE Uncultured Chlorophyta clone KC / RS.12f.10m.53 CC02SE Uncultured Prasinophyceae clone FJ / Biosope_T CC02A Uncultured Chlorophyta clone KC / RS.12f.10m.0069 CC02SE Uncultured Prasinophyceae clone FJ / Biosope_T CC02SE Uncultured Prasinophyceae clone FJ / Biosope_T CC02A Pycnococcus provasolii FR / CC02SE Uncultured Prasinophyceae clone FJ / Biosope_T CC02SE Uncultured Prasinophyceae clone FJ / Biosope_T CC02A Uncultured prasinophyte clone BL AY /

5 CC02SE Bathycoccus prasinos isolate CCMP1898 JX / Fungi CC02A Fungi Meira sp. JCM AB / CC02SE Tetracladium marchalianum AF / Haptophyta CC02A CC02A Prymnesiophyceae Phaeocystis globosa isolate CCMP1524 Uncultured marine haptophyte clone Biosope_T Rhizaria CC02A Cercozoa DSGM-58 AF / FJ / AB / CC02A Radiolaria Astrosphaera hexagonalis AB / CC02A Uncultured marine Polycystinea EU / clone OLI011-75m.62 CC02A Chaunacanthid sp. 218 AF / CC02A Uncultured Polycystinea clone FJ / Biosope_T CC02A Haliommatidium sp. AF / CC02A clone MO GU / CC02SE Uncultured marine Polycystinea clone OLI011-75m.50 EU / CC02A Astrosphaera hexagonalis AB / CC02A Uncultured marine Polycystinea EU / clone OLI011-75m.47 CC02A Spongodiscus resurgens isolate AB / CC02A Trizona brandti isolate Ros6 JN / CC02SE AY / SCM38C4 CC02SE Uncultured taxopodid-like clone DQ / LC22_5EP_23 CC02SE Hexacontium pachydermum AB / CC02SE Uncultured marine Polycystinea clone OLI011-75m.62 EU / Stramenopiles CC02A Labyrinthulea Uncultured stramenopile clone 905st23-36 JQ /

6 CC02A clone HE AY / CC02SE Eukaryote marine clone ME1-24 AF / CC02SE MAST clone BL AY / CC02A Uncultured heterokont clone DQ / LC23_5EP_40 CC02SE Uncultured stramenopile clone JQ / D3Ae6e7 CC02A Uncultured heterokont clone DQ / LC23_5EP_40 CC02A Uncultured stramenopile clone JQ / D3Ae6i8 CC02A Picobiliphytes DQ / clone NW Fig. S1. Distribution of salinity (PSU) in the northern South China Sea (SCS) at the surface (A) and 75m (B), during the overall CHOICE-C winter cruise. Dots correspond to stations sampled 6

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