Delineation of IRZs and PRZs in the German license area (CCZ): Criteria and characteristics

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1 Delineation of IRZs and s in the German license area (CCZ): Criteria and characteristics Annemiek Vink, Carsten Rühlemann, Thomas Kuhn BGR Hannover, Germany Annika Janssen, Katja Uhlenkott DZMB Wilhelmshaven, Germany 1

2 Nodule license areas in the CCZ Mexico Hawaii Exploration contract July 2006 July expeditions ( ); next expedition spring

3 Prospective mining areas Hard rock, slope > 3 (27%) Large nodules (9%) Small nodules (64%) Prospective areas (18%) IRZ PA2 PA km 3

4 IRZ: BGR Bottom ADCP current moorings data April 2013 May 2014 May 2014 June 2015 June 2015 May

5 IRZ: BGR Plume ADCP modelling moorings For PA1 (70 x 70 km): 3D hydrodynamic Ocean model + Sediment-transport model 0.1 mm Sediment mobilisation: 1000 t/h 10 mm 1 mm 5

6 Box core samples IRZ PA1 PA2 6

7 Macrofauna IRZ vs. nmds based on Bray-Curtis Similarity North IRZ Significantly higher densities in the total densities in eastern German license area: ind./ 0.25 m² Small but siginificant differences in macrobenthic community composition 7

8 Macrofauna IRZ vs. IRZ Ophiuroidea Polychaeta Peracarida 8 No significant differences in community structure between IRZ and at higher taxonomic level a Impact Reference Zone (IRZ) a a Preservation Reference Zone () Bivalvia Maxillopoda Isopoda

9 Small-scale connectivity Polychaeta*: 550 sequences 147 MOTUs 67 MOTUs singletons (46%) 40 MOTUs shared (27%) 35 MOTUs in 5 MOTUs in IRZ * Mitochondrial Cytochromoxidase Subunit 1 (COI) gene Janssen et al. (in prep.): nmds based on BrayCurtis Similarity Raschka (2014), Master thesis 9

10 Small-scale connectivity Isopods: 349 sequences 83 MOTUs 45 MOTUs singletons (54%) 11 MOTUs shared (13%) 15 MOTUs 12 MOTUs IRZ Janssen et al. (in prep.): nmds based on BrayCurtis Similarity Raschka (2014), Master thesis 10 Max. one-third of the putative species live in both areas phylogenetic differences within one area are almost as high as between areas

11 Genetic structure between populations 11 Janssen et al. (submitted) No geographic population structure: - hardly divergence - sufficient gene flow

12 Multicorer samples IRZ PA2 12

13 Meiofauna IRZ vs. Significantly higher densities in the IRZ No siginificant differences in meiobenthic community structure at higher taxonomic level 13

14 Meiofauna total abundance from point sampling to spatial mapping R-Package Random-Forest (machine-learning algorithm) PA2 PA1 Mapping based on: Bathymetry Backscatter Mn-nodule coverage 0.44 of variation explained 14

15 Meiofauna diversity (Simpson Index) PA2 PA1 Mapping based on: Bathymetry Backscatter Mn-nodule coverage 0.32 of variation explained 15

16 Nodule size variability PA1 PA2 16

17 Geochemical BGR ADCP variability moorings of nodules PA2 PA1 17

18 Geochemical variability of nodules PA1 PA2 Cu > Ni 18

19 TOC concentrations PA1 PA2 19

20 MnO 2 content PA1 PA2 20

21 What have we learnt? is a functional area for: ensuring representative and stable biota of the seabed as a protected area with a wide range of nodule facies and habitats But: high degree of spatial variability in geological, geochemical and ecological conditions (topography, nodule composition, size, coverage, biodiversity) between and within reference areas at small scales So at the size scales of IRZs and s (> 50 km apart), the definition of a as being as ecologically similar as possible to the impact zone, and removed from potential mining impacts (CCZ EMP) may be a difficult goal to reach! 21

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