Cell Culture Powder Media

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1 Cell Culture Powder Media g Comment: Composition HS-No.: 3822 Storage: 2-8 C LGK: S: 24/25 hygroscopic Powder media from AppliChem are mixed and processed according to the original formulations. To produce these media only raw materials of high quality are used. The single raw material that is used in the media production is taken only from one lot, which is dried according to instructions. Shortly, after drying the raw materials, they will be grinded in special mills to get a homogenous fine powder, that is packed immediately. Due to that the customer can easily dissolve our media. You will receive a weighing protocol on request. HEPES-buffered media HEPES-buffered media are often used, whenever the ph-value has to be stable in the media. With the addition of HEPES the ph value will get an additional stability in the ph range 7.2 to 7.6 for ph-sensitive cell culture systems. Deviations of the ph value in the media are caused by the metabolism of the cultivated cells but also by changes of the CO 2 concentration during incubation. Storage All powder media are produced without sodium hydrogen carbonate to increase the stability. Keeping them tightly closed is absolutely necessary as they are very hygroscopic. For this reason one package of powder media should always be dissolved completely. Storing dry and cold (+4 to +8 C) will increase the shelf life, which is normally two years. Generally the time for delivery will be one week. Customer formulations As a special service AppliChem offers the production of powder media according to the customer formulation for competitive prices - even in smaller quantities. In our laboratories we are able to consider most wishes regarding the composition of the powder media. The minimum order size is 20 liters with a delivery-date of two to three weeks, generally. Please ask for our offer for your formulation giving us the exact composition and the quantity you need. Literature ME: Eagle, H. (1965) Proc. Soc. Exp. iol. Med. 89, 362 DMEM: Dulbecco, R. & Freeman, G. (1959) Virology 8, 396 Smith et al. (1960) Virology 12, 185 DMEM/F12: Dulbecco, R. & Freeman, G. (1959) Virology 8, 396 Smith et al. (1960) Virology 12, 185 arnes, D. & Sato, G. (1980) Anal. iochem. 102, 255 Earle's buffered salt mixture: Earle, W. (1943) J. Nat. Cancer Inst.4, 165 Gamborg's 5 - Plant salts: Gamborg O.L., Miller R.A., OjimaK. (1968) Exp. Cell Res. 50,151 Glasgow - MEM (HK 21): MacPherson, I. & Stoker, M. (1961) Virology 16, 147 Grace's - Insect medium: Grace, T.D.C. (1962) Nature 195, 788 Grace, T.D.C. (1962) Nature216, 613 Grace, T.D.C. (1966) Nature 211, 366 Ham's F10 - Medium: Ham, R.G. (1963) Exp. Cell Res. 29, 515 Ham's F12K - Medium: Kaighn, M.E., (1973) Tissue Culture Methods and Applications, 54 (eds. Kruse & Patterson) Ham's F12 - Medium: Ham, R.G. (1965) Proc. Nat. Acad. Sci USA 53, 288 Hanks' buffered salt mixture: Hanks', J.H. & Wallace, R.E. (1949) Proc. Soc. Exp. iol. Med. 71, 196. Iscove's mod. Dulbecco's MEM (IMDM): Iscove, N., N. & Melchers, F (1978) J. Exp. Med. 147, 926 Iscove, N., N. et al. (1980) Exp. Cell Res. 126, 121 IPL-41 - Medium: Weiss, S.A., G.C. Smith, S.S. Kalter & J.L. Vaughn. (1981) In Vitro 17, 495. Joklik - MEM: Eagle, H. (1959) Science 130, 432 Leibovitz's L15 - Medium: Leibovitz, A. (1963) Amer. J. Hyg. 78, 173 Linsmaier, ednar & Skoog - Plant salts: Linsmaier E.M. & Skoog F. (1965) Physiol. Plantarum 18, 1 McCoy's 5A - Medium (modified): McCoy, T.A. et al. (1959) Proc. Soc. Exp. iol. Med. 1, 115 Hsu, T.C. & Kellogg, D.S. (1960) J. Nat. Cancer Inst.,25, 221 Iwakata, S. & Grace, J.T. (1964) N.Y. J. Med. 64, 2279 Medium 199: Morgan, J.F. et al. (1950) Proc. Soc. Exp. iol. Med. 73, 1 Morgan, J.F. et al. (1955) J. Nat. Cancer Inst. 16, 557 a-mem: Stanners, C.P. et al. (1971) Nature New iology 230, 52 Stanners, C.P. & Goldberg, V.J. (1975) J. Gen. Virol. 29, 281 MEM: Eagle, H. (1955) Science 122, 501 Eagle, H. (1959) Science 130, 432 S-MEM: Eagle, H. (1959) Science 130, 432 Murashige & Skoog - Plant medium: Murashige, T. & Skoog, F. (1962) Physiol. Plant. 15, 473 NCTC Medium: Evans, V.J. et al. (1964) Exp. Cell Res. 36, 439 PS - Powder: Dulbecco, R. & Vogt, M. (1957) J. Exp. Med. 106, 167 RPMI Medium: Moore, G.E. et al. (1967) J.A.M.A. 199, 519 Schneider's Drosophila - Insect medium: Schneider, I. & lumenthal, A. (1978) iology and Genetics of Drosophila vol. 2A, Acad. Press, N.Y. pp TC-1 - Insect medium: Gardiner, G.R. & H. Stockdale (1975) J. Invert. Pathol. 25, 363 TNM-FH - Insect medium: Hink, W.F. (1970) Nature 266, 466 Hink, W.F. et al. (1974) In Vitro 9, 371 Waymouth's M 752/1 - Medium: Waymouth, C. (1959) J. Nat. Cancer Inst. 22, 13 William's Medium E: Williams, G.M. et al. (1971) Exp. Cell Res. 69, 106 Williams, G.M. & Gunn, J.M. (1974) Exp. Cell Res. 89, 139 Instructions for dissolving the powder to prepare liquid media Powdered media and salts are very hygroscopic and have to be stored under dry conditions. After opening one package you should dissolve the whole content at once. We recommend to produce 1X liquid medium from the powdered form, as the different amino acids may precipitate because of their low solubility. They are able to form salts which are of low solubility in concentrated solutions. If you have to add supplements to your medium, you can add them before filtration (unsterile) or after filtration (sterile). For dissolving the powdered medium please use bidistilled or deionized, pyrogen-free water. Procedure The required quantity of powdered medium will be completely dissolved under stirring in approx. 90 % of the water quantity. The temperature of the water should be between +15 and +30 C. The powder which remains in the package should be flushed out and dissolved with water. If the powder is completely dissolved, add the sodium hydrogen carbonate and dissolve it completely as well. The required quantities are listed in the table on the following pages. The ph (physiological optimum is ph ) has to be adjusted with 1 N HCl or 1 N NaOH while stirring. The ph should be approx. 0.2 units lower than the target ph, because the ph will raise up a bit during filtration when the CO2 will leak out. After adjusting the ph you have to add water up to the desired volume and mix it very well and filter immediately. Store the medium at +4 C protected from light. 727

2 ME, asal medium with Earle's salts ME, asal medium with Earle's salts ME, asal medium with Earle's salts 10X ME, asal medium with Earle's salts 10X ME, asal medium with Earle's salts ME, asal medium with Earle's salts (diploide) ME, asal medium with Earle's salts (diploide) ME, asal medium with Earle's salts, autoclavable ME, asal medium with Earle's salts ME, asal medium with Earle's salts A1310,10 1L A1310,50 5L A1310, L A1310, L A1311,10 1L A1311,50 5L A1311, L A1311, L A1977,10 1L A1977,50 5L A1977, L A1977, L A1983,10 1L A1983,50 5L A1983, L A1983, L A1984,10 1L A1984,50 5L A1984, L A1984, L A1312,10 1L A1312,50 5L A1312, L A1312, L A1976,10 1L A1976,50 5L A1976, L A1976, L A1915,10 1L A1915,50 5L A1915, L A1915, L A1916,10 1L A1916,50 5L A1916, L A1916, L 728

3 ME, asal medium with Hanks' salts ME, asal medium with Hanks' salts ME, asal medium with Hanks' salts 10X ME, asal medium with Hanks' salts 10X ME, asal medium with Hanks' salts ME, asal medium with Hanks' salts, autoclavable ME, asal medium with Hanks' salts ME, asal medium with Hanks' salts DMEM (Dulbecco's mod. Eagle - Media) A1917,10 1L A1917,50 5L A1917, L A1917, L A1313,10 1L A1313,50 5L A1313, L A1313, L A1990,10 1L A1990,50 5L A1990, L A1990, L A1988,10 1L A1988,50 5L A1988, L A1988, L A1978,10 1L A1978,50 5L A1978, L A1978, L A1918,10 1L A1918,50 5L A1918, L A1918, L A1919,10 1L A1919,50 5L A1919, L A1919, L DMEM (Dulbecco's mod. Eagle - Medium) with 1.0 g/l D(+)-Glucose with Sodium pyruvate DMEM (Dulbecco's mod. Eagle - Medium) with 1.0 g/l D(+)-Glucose with Sodium pyruvate A1924,10 1L A1924,50 5L A1924, L A1924, L A1314,10 1L A1314,50 5L A1314, L A1314, L 729

4 DMEM (Dulbecco's mod. Eagle - Medium) with 1.0 g/l D(+)-Glucose with Sodium pyruvate without Phenol red DMEM (Dulbecco's mod. Eagle - Medium) with 1.0 g/l D(+)-Glucose with Sodium pyruvate without Phenol red DMEM (Dulbecco's mod. Eagle - Medium) with 1.0 g/l D(+)-Glucose with Sodium pyruvate DMEM (Dulbecco's mod. Eagle - Medium) with 1.0 g/l D(+)-Glucose with Sodium pyruvate DMEM (Dulbecco's mod. Eagle - Medium) with 4.5 g/l D(+)-Glucose without Sodium pyruvate DMEM (Dulbecco's mod. Eagle - Medium) with 4.5 g/l D(+)-Glucose without Sodium pyruvate DMEM (Dulbecco's mod. Eagle - Medium) with 4.5 g/l D(+)-Glucose with Sodium pyruvate DMEM (Dulbecco's mod. Eagle - Medium) with 4.5 g/l D(+)-Glucose with Sodium pyruvate DMEM (Dulbecco's mod. Eagle - Medium) with 4.5 g/l D(+)-Glucose with Sodium pyruvate without Phenol red DMEM (Dulbecco's mod. Eagle - Medium) with 4.5 g/l D(+)-Glucose with Sodium pyruvate without Phenol red A0826,10 1L A0826,50 5L A0826, L A0826, L A3134,10 1L A3134,50 5L A3134, L A3134, L A1925,10 1L A1925,50 5L A1925, L A1925, L A1315,10 1L A1315,50 5L A1315, L A1315, L A1926,10 1L A1926,50 5L A1926, L A1926, L A1316,10 1L A1316,50 5L A1316, L A1316, L A1985,10 1L A1985,50 5L A1985, L A1985, L A1986,10 1L A1986,50 5L A1986, L A1986, L A3137,10 1L A3137,50 5L A3137, L A3137, L A3139,10 1L A3139,50 5L A3139, L A3139, L 730

5 DMEM (Dulbecco's mod. Eagle - Medium) with 4.5 g/l D(+)-Glucose without Sodium pyruvate DMEM (Dulbecco's mod. Eagle - Medium) with 4.5 g/l D(+)-Glucose without Sodium pyruvate DMEM (Dulbecco's mod. Eagle - Medium) with 4.5 g/l D(+)-Glucose with Sodium pyruvate DMEM (Dulbecco's mod. Eagle - Medium) with 4.5 g/l D(+)-Glucose with Sodium pyruvate DMEM/F12 (1:1) - Media A1950,10 1L A1950,50 5L A1950, L A1950, L A1965,10 1L A1965,50 5L A1965, L A1965, L A1979,10 1L A1979,50 5L A1979, L A1979, L A1951,10 1L A1951,50 5L A1951, L A1951, L DMEM/F12 (1:1) - Medium DMEM/F12 (1:1) - Medium DMEM/F12 (1:1) - Medium with 15 mm HEPES DMEM/F12 (1:1) - Medium with 15 mm HEPES DMEM/F12 (1:1) - Medium DMEM/F12 (1:1) - Medium A1920,10 1L A1920,50 5L A1920, L A1920, L A1318,10 1L A1318,50 5L A1318, L A1318, L A1922,10 1L A1922,50 5L A1922, L A1922, L A1317,10 1L A1317,50 5L A1317, L A1317, L A1923,10 1L A1923,50 5L A1923, L A1923, L A1921,10 1L A1921,50 5L A1921,9010N 10 L A1921, L 731

6 Earle's balanced salts (ESS) Earle's balanced salts (ESS) with 1.0 g/l D(+)-Glucose with Phenol red Earle's balanced salts (ESS) with 1.0 g/l D(+)-Glucose without Phenol red Earle's balanced salts (ESS) with 1.0 g/l D(+)-Glucose with Phenol red without Calcium and Magnesium Earle's balanced salts (ESS) with 1.0 g/l D(+)-Glucose without Calcium and Magnesium without Phenol red Gamborg's 5 Plant salts A1712,50 5L A1712, L A1712, L A2039,50 5L A2039, L A2039, L A3026,50 5L A3026, L A3026, L A3127,50 5L A3127, L A3127, L A1911,50 5L A1911, L A1911, L Glasgow - MEM (HK 21) Glasgow - MEM (HK 21) without Tryptose/Phosphate-ouillon Glasgow - MEM (HK 21) without Tryptose/Phosphate-ouillon Glasgow - MEM (HK 21) with Tryptose/Phosphate-ouillon Glasgow - MEM (HK 21) with Tryptose/Phosphate-ouillon A2024,10 1L A2024,50 5L A2024, L A2024, L A1320,10 1L A1320,50 5L A1320, L A1320, L A2025,10 1L A2025,50 5L A2025, L A2025, l A1321,10 1L A1321,50 5L A1321, L A1321, L 732

7 Grace's - Insect medium Grace's - Insect medium Grace's - Insect medium Ham's F10 - Medium A2023,10 1L A2023,50 5L A2023, L A2023, L A1529,10 1L A1529,50 5L A1529, L A1529, L Ham's F10 - Medium Ham's F10 - Medium Ham's F10 - Medium Ham's F10 - Medium Ham's F10 - Medium with 1.2 g/l Sodium hydrogen carbonate Ham's F10 - Medium with 1.2 g/l Sodium hydrogen carbonate Ham's F12 - Medium A1970,10 1L A1970,50 5L A1970, L A1970, L A1525,10 1L A1525,50 5L A1525, L A1525, L A1989,10 1L A1989,50 5L A1989, L A1989, L A1982,10 1L A1982,50 5L A1982, L A1982, L A2026,10 1L A2026,50 5L A2026, L A2026, L A1952,10N 1L A1952,50 5L A1952, L A1952, L Ham's F12 - Medium A1948,10 1L A1948,50 5L A1948, L A1948, L 733

8 Ham's F12 - Medium Ham's F12 - Medium Ham's F12 - Medium Ham's F12K - Medium A1526,10 1L A1526,50 5L A1526, L A1526, L A1969,10 1L A1969,50 5L A1969, L A1969, L A1953,10 1L A1953,50 5L A1953, L A1953, L Ham's F12K - Medium Ham's F12K - Medium Hanks' balanced salts (HSS) A1963,10 1L A1963,50 5L A1963, L A1963, L A1927,10 1L A1927,50 5L A1927, L A1927, L Hanks' balanced salts (HSS) with 1.0 g/l D(+)-Glucose with Phenol red Hanks' balanced salts (HSS) with 1.0 g/l D(+)-Glucose without Phenol red Hanks' balanced salts (HSS) with 1.0 g/l D(+)-Glucose without Calcium and Magnesium with Phenol red Hanks' balanced salts (HSS) with 1.0 g/l D(+)-Glucose without Calcium and Magnesium without Phenol red HEPES - balanced salts A1711,50 5L A1711, L A1711, L A3140,50 5L A3140, L A3140, L A2729,50 5L A2729, L A2729, L A2512,50 5L A2512, L A2512, L A0629,10 1L A0629,50 5L A0629, L 734

9 IPL 41 - Medium IPL 41 - Medium IPL 41 - Medium Iscove's mod. Dulbecco's - Medium (IMDM) A2027,10 1L A2027,50 5L A2027, L A2027, L A1964,10 1L A1964,50 5L A1964, L A1964, L Iscove's mod. Dulbecco's - Medium (IMDM) Iscove's mod. Dulbecco's - Medium (IMDM) Iscove's mod. Dulbecco's - Medium (IMDM) Iscove's mod. Dulbecco's - Medium (IMDM) Joklik - MEM, modified for spinner culture A2028,10 1L A2028,50 5L A2028, L A2028, L A1909,10 1L A1909,50 5L A1909, L A1909, L A2029,10 1L A2029,50 5L A2029, L A2029, L A1322,10 1L A1322,50 5L A1322, L A1322, L Joklik - MEM, modified for spinner culture with Earle's salt (modified) without Antibiotics without Calcium chloride Joklik - MEM, modified for spinner culture with Earle's salts (modified) without Antibiotics without Calcium chloride A1974,10 1L A1974,50 5L A1974, L A1974, L A1539,10 1L A1539,50 5L A1539, L A1539, L 735

10 Leibovitz's L15 - Medium Leibovitz's L15 - Medium Leibovitz's L15 - Medium Leibovitz's L15 - Medium Leibovitz's L15 - Medium Linsmaier, ednar & Skoog Plant salts A1967,10 1L A1967,50 5L A1967, L A1967, L A1323,10 1L A1323,50 5L A1323, L A1323, L A1968,10 1L A1968,50 5L A1968, L A1968, L A1954,10 1L A1954,50 5L A1954, L A1954, L A0591,50 5L A0591, L A0591, L McCoy's 5A - Medium (modified) McCoy's 5A - Medium (modified) without Serum McCoy's 5A - Medium (modified) without Serum McCoy's 5A - Medium (modified) without Serum McCoy's 5A - Medium (modified) without Serum A2034,10 1L A2034,50 5L A2034, L A2034, L A1324,10 1L A1324,50 5L A1324, L A1324, L A2035,10 1L A2035,50 5L A2035, L A2035, L A1955,10 1L A1955,50 5L A1955, L A1955, L 736

11 Medium 199 with Earle's Salts Medium 199 with Earle's Salts Medium 199 with Earle's Salts Medium 199 with Earle's Salts Medium 199 with Earle's Salts Medium 199 with Hanks' Salts A2022,10 1L A2022,50N 5L A2022, L A2022, L A1325,10 1L A1325,50 5L A1325, L A1325, L A2030,10 1L A2030,50 5L A2030, L A2030, L A1326,10 1L A1326,50 5L A1326, L A1326, L Medium 199 with Hanks' Salts Medium 199 with Hanks' Salts Medium 199 with Hanks' Salts Medium 199 with Hanks' Salts a-mem A2031,10 1L A2031,50 5L A2031, L A2031, L A1327,10 1L A1327,50 5L A1327, L A1327, L A2021,10 1L A2021,50 5L A2021, L A2021, L A1328,10 1L A1328,50 5L A1328, L A1328, L a-mem without Deoxyribonucleosides without Ribonucleosides A1971,10 1L A1971,50 5L A1971, L A1971, L 737

12 a-mem without Deoxyribonucleosides without Ribonucleosides a-mem with Ribonucleosides with Deoxyribonucleosides a-mem with Deoxyribonucleosides with Ribonucleosides a-mem without Deoxyribonucleosides without Ribonucleosides a-mem without Deoxyribonucleosides without Ribonucleosides a-mem with Deoxyribonucleosides with Ribonucleosides a-mem with Desoxyribonucleosides with Ribonucleosides MEM with Earle's salts A1330,10 1L A1330,50 5L A1330, L A1330, L A1913,10 1L A1913,50 5L A1913, L A1913, L A1329,10 1L A1329,50 5L A1329, L A1329, L A1980,10 1L A1980,50 5L A1980, L A1980, L A1972,10 1L A1972,50 5L A1972, L A1972, L A1949,10 1L A1949,50 5L A1949, L A1949, L A1914,10 1L A1914,50 5L A1914, L A1914, L MEM with Earle's salts MEM with Earle's salts MEM with Earle's salts, autoclavable A1331,10 1L A1331,50 5L A1331, L A1331, L A1332,10 1L A1332,50 5L A1332, L A1332, L A2597,10 1L A2597,50 5L A2597, L A2597, L 738

13 MEM with Earle's salts with NEAA MEM with Earle's salts with NEAA MEM with Earle's salts MEM with Earle's salts MEM with Earle's salts with NEAA MEM with Earle's salts with NEAA MEM with Hanks' salts A2018,10N 1L A2018,50 5L A2018,9010N 10 L A2018, L A1334,10 1L A1334,50 5L A1334, L A1334, L A2019,10 1L A2019,50 5L A2019, L A2019, L A1333,10 1L A1333,50 5L A1333, L A1333, L A2032,10 1L A2032,50 5L A2032, L A2032, L A1956,10 1L A1956,50 5L A1956, L A1956, L MEM with Hanks' salts MEM with Hanks' salts MEM with Hanks' salts with NEAA MEM with Hanks' salts with NEAA A1957,10 1L A1957,50 5L A1957, L A1957, L A1335,10 1L A1335,50 5L A1335, L A1335, L A2041,10 1L A2041,50 5L A2041, L A2041, L A1337,10 1L A1337,50 5L A1337, L A1337, L 739

14 MEM with Hanks'salts MEM with Hanks' salts MEM with Hanks' salts with NEAA MEM with Hanks' salts with NEAA S-MEM, modified for spinner culture A2042,10 1L A2042,50 5L A2042, L A2042, L A1336,10 1L A1336,50 5L A1336, L A1336, L A2043,10 1L A2043,50 5L A2043, L A2043, L A1958,10 1L A1958,50 5L A1958, L A1958, L S-MEM, modified for spinner culture with Earle's salt (modified) S-MEM, modified for spinner culture with Earle's salts (modified) S-MEM, modified for spinner culture, autoclavable with Earle's salt (modified) autoclavable NCTC Medium A1338,10 1L A1338,50 5L A1338, L A1338, L A1308,10 1L A1308,50 5L A1308, L A1308, L A1975,10 1L A1975,50 5L A1975, L A1975, L NCTC Medium NCTC Medium A2020,10 1L A2020,50 5L A2020, L A2020, L A1528,10 1L A1528,50 5L A1528, L A1528, L 740

15 PS buffer (Dulbecco's) - Powder Phosphate buffered saline, Phosphate buffered saline, 10X concentrated PS buffer (1X, Dulbecco's) - Powder PS buffer (10X Dulbecco's) - Powder PS buffer (1X Dulbecco's) - Powder with 0,01 % Thimerosal with 0,02 % EDTA RPMI Medium A0964, L A0964, L A0964,91 1 L A0965, L A0965, L A0965,91 1 L A1908, L A1908, L A1908,91 1 L RPMI Medium RPMI Medium RPMI Medium without Phenol red RPMI Medium without Phenol red RPMI Medium RPMI Medium A1959,10 1L A1959,50 5L A1959, L A1959, L A1339,10 1L A1339,50 5L A1339, L A1339, L A2040,10 1L A2040,50 5L A2040, L A2040, L A3135,10 1L A3135,50 5L A3135, L A3135, L A1960,10 1L A1960,50 5L A1960, L A1960, L A1538,10 1L A1538,50 5L A1538, L A1538, L 741

16 Click - RPMI - Medium Click - RPMI - Medium Click - RPMI - Medium Schneider's Drosophila - Insect medium A2044,10 1L A2044,50 5L A2044, L A2044, L A2504,10 1L A2504,50 5L A2504, L A2504, L Schneider's Drosophila - Insect medium Schneider's Drosophila - Insect medium TC 1 - Insect medium A2036,10N 1L A2036,50 5L A2036,9010N 10 L A2036, L A1533,10 1L A1533,50 5L A1533, L A1533, L TC 1 - Insect medium A2033,10 1L A2033,50 5L A2033, L A2033, L TC 1 - Insect medium TNM-FH - Insect medium (modifiziertes Grace's Insektenmedium) A2017,10 1L A2017,50 5L A2017, L A2017, L modified Grace's Insect medium TNM-FH - Insect medium (modified Grace's Insect medium) with Lactalbumin hydrolysate with Yeast extracts TNM-FH - Insect medium (modified Grace's Insect medium) with Lactalbumin hydrolysate with Yeast extracts A2037,10 1L A2037,50 5L A2037, L A2037, L A1570,10 1L A1570,50 5L A1570, L A1570, L 742

17 Waymouth's M 752/1 - Medium Waymouth's M 752/1 - Medium Waymouth's M 752/1 - Medium Waymouth's M 752/1 - Medium with 20 mm HEPES Waymouth's M 752/1 - Medium with 20 mm HEPES William's Media E A1973,10 1L A1973,50 5L A1973,9010N 10 L A1973, L A1527,10 1L A1527,50 5L A1527, L A1527, L A1981,10 1L A1981,50 5L A1981, L A1981, L A1961,10 1L A1961,50 5L A1961, L A1961, L William's Medium E William's Medium E William's Medium E without Phenol red William's Medium E without Phenol red William's Medium E A1309,10 1L A1309,50 5L A1309, L A1309, L A1910,10 1L A1910,50 5L A1910, L A1910, L A3029,10 1L A3029,50 5L A3029, L A3029, L A3136,10 1L A3136,50 5L A3136, L A3136, L A1966,10N 1L A1966,50 5L A1966, L A1966, L 743

18 William's Medium E A1962,10 1L A1962,50 5L A1962, L A1962, L 744

19 Table for the addition of sodium hydrogen carbonate Description Article-No. NaHCO3 (g / L) ME, asal medium with Earle's salts A ME, asal medium with Earle's salts A X ME, asal medium with Earle's salts A / L 1X 10X ME, asal medium with Earle's salts A / L 1X ME, asal medium with Earle's salts, with HEPES A ME, asal medium with Earle's salts, with HEPES A ME, asal medium with Earle's (diploide) A ME, asal medium with Earle's (diploide) A ME, asal medium with Hanks' salts A ME, asal medium with Hanks' salts A X ME, asal medium with Hanks' salts A / L 1X 10X ME, asal medium with Hanks' salts A / L 1X ME, asal medium with Hanks' salts; autoclavable A ml 7.5 % - Solution / L after autoclaving ME, asal medium with Hanks' salts; autoclavable A ml 7.5 % - Solution / L after autoclaving ME, asal medium with Hanks' salts, with HEPES A ME, asal medium with Hanks' salts, with HEPES A DMEM - Medium A DMEM - Medium A DMEM - Medium with HEPES A DMEM - Medium with HEPES A DMEM - Medium A DMEM - Medium A DMEM - Medium A DMEM - Medium A DMEM - Medium with HEPES A DMEM - Medium with HEPES A DMEM - Medium with HEPES A DMEM - Medium with HEPES A DMEM - Medium A DMEM - Medium A DMEM - Medium A DMEM - Medium A DMEM/F12 (1:1) - Medium A DMEM/F12 (1:1) - Medium A DMEM/F12 (1:1) - Medium with 15 mm HEPES A DMEM/F12 (1:1) - Medium with 15 mm HEPES A DMEM/F12 (1:1) - Medium A DMEM/F12 (1:1) - Medium A Earle's balanced salts A Earle's balanced salts A Earle's balanced salts A Earle's balanced salts A Gamborg's 5 - Plant salts A1911 none Glasgow - MEM (HK 21) A Glasgow - MEM (HK 21) A Glasgow - MEM (HK 21) A Glasgow - MEM (HK 21) A Grace's - Insect medium A Grace's - Insect medium A Ham's F10 - Medium A Ham's F10 - Medium A Ham's F10 - Medium with HEPES A Ham's F10 - Medium with HEPES A Ham's F10 - Medium with HEPES A Ham's F10 - Medium with HEPES A Ham's F12K - Medium A Ham's F12K - Medium A Ham's F12 - Medium A Ham's F12 - Medium A Ham's F12 - Medium with HEPES A Ham's F12 - Medium with HEPES A Ham's F12K - Medium A Ham's F12K - Medium A Hanks' balanced salts A Hanks' balanced salts A

20 Table for the addition of sodium hydrogen carbonate Description Article-No. NaHCO3 (g / L) Hanks' balanced salts A Hanks' balanced salts A HEPES balanced salts A0629 none Iscove's mod. Dulbecco's - Medium (IMDM) A Iscove's mod. Dulbecco's - Medium (IMDM) A Iscove's mod. Dulbecco's - Medium (IMDM) A Iscove's mod. Dulbecco's - Medium (IMDM) A IPL 41 - Medium A IPL 41 - Medium A Joklik - MEM, modified for spinner culture A Joklik - MEM, modified for spinner culture A Leibovitz's L-15 - Medium A1967 none Leibovitz's L-15 - Medium A1323 none Leibovitz's L-15 - Medium with HEPES A1968 none Leibovitz's L-15 - Medium with HEPES A1954 none Linsmaier, ednar & Skoog Plant salts A0591 none McCoy's 5A - Medium (modified) A McCoy's 5A - Medium (modified) A McCoy's 5A - Medium (modified) A McCoy's 5A - Medium (modified) A Medium 199 with Earle's salts A Medium 199 with Earle's salts A Medium 199 with Earle's salts, with HEPES A Medium 199 with Earle's salts, with HEPES A Medium 199 with Hanks' salts A Medium 199 with Hanks' salts A Medium 199 with Hanks' salts, with HEPES A Medium 199 with Hanks' salts, with HEPES A a - MEM A a - MEM A a - MEM A a - MEM A a - MEM with HEPES A a - MEM with HEPES A a - MEM with HEPES A a - MEM with HEPES A MEM with Earle's salts A MEM with Earle's salts A MEM with Earle's salts A MEM with Earle's salts A MEM with Earle's salts, with HEPES A MEM with Earle's salts, with HEPES A MEM with Earle's salts, with HEPES A MEM with Earle's salts, with HEPES A MEM with Earle's salts; autoclavable A ml 7.5 % - Solution / L after autoclaving MEM with Hanks' salts A MEM with Hanks' salts A MEM with Hanks' salts A MEM with Hanks' salts A MEM with Hanks' salts, with HEPES A MEM with Hanks' salts, with HEPES A MEM with Hanks' salts, with HEPES A MEM with Hanks' salts, with HEPES A S-MEM, modified for spinner culture A S-MEM, modified for spinner culture A S-MEM, modified for spinner culture; autoclavable A ml 7.5 % - Solution / L after autoclaving Murashige & Skoog - Plant salts A1534 none Murashige & Skoog - Plant salts A1535 none NCTC Medium A NCTC Medium A PS buffer (1X, Dulbecco) - Powder A0964 none PS buffer (10X, Dulbecco) - Powder A0965 none PS buffer (1X, Dulbecco) - Powder A1908 none RPMI 1640 A RPMI 1640 A

21 Table for the addition of sodium hydrogen carbonate Description Article-No. NaHCO3 (g / L) RPMI 1640 with HEPES A RPMI 1640 with HEPES A RPMI 1640 A RPMI 1640 A Click-RPMI A Click-RPMI A Schneider's Drosophila - Insect medium A Schneider's Drosophila - Insect medium A TC-1 - Medium A TC-1 - Medium A TNM-FH - Insect medium A TNM-FH - Insect medium A Tris - Citrate balanced salts A0692 none Waymouth's M 752/1 - Medium A Waymouth's M 752/1 - Medium A Waymouth's M 752/1 - Medium with 20 mm HEPES A Waymouth's M 752/1 - Medium with 20 mm HEPES A William's Medium E A William's Medium E A William's Medium E with HEPES A William's Medium E with HEPES A William's Medium E A William's Medium E A

22 ME, asal medium with Earle's salts Inorganic salts Other Components Components A1310 A1311 A1977 A1983 A1915 A1916 A1984 A1312 A1976 autoclavable Calcium chloride x2h 2O Magnesium chloride anhydrous Magnesium 139,52 139, , ,20 139,52 139,52 139,52 sulfate dried Potassium chloride Sodium chloride Sodium dihydrogen phosphate x H2O D(+)-Glucose anhydrous HEPES Phenol red Succinic acid 75. Succinic acid sodium salt 1. Amino acids L-Arginine x HCl L-Cystine L-Glutamine L-Histidine ase L-Histidine x HCl xh2o L-Isoleucine L-Leucine L-Lysine x HCl L-Methionine L-Phenylalanine L-Threonine L-Tryptophan L-Tyrosine L-Valine Vitamins D(+)-iotin D-Calcium pantothenate Choline bitartrate 1.80 Choline chloride Folic acid myo-inositol Nicotinamide Pyridoxal x HCl Riboflavin Thiamine x HCl Weight (g / L)

23 ME, asal medium with Hanks' salts Inorganic salts Other Components Components A1917 A1313 A1990 A1988 A1918 A1919 A1978 autoclavable Calcium chloride x2h 2O Magnesium sulfate dried Potassium chloride Potassium dihydrogen phosphate anhydrous Sodium chloride di-sodium hydrogen phosphate D(+)-Glucose anhydrous HEPES Phenol red Succinic acid 75. Succinic acid sodium salt 1. Amino acids L-Arginine x HCl L-Cystine L-Glutamine L-Histidine ase L-Histidine x HCl 15. xh2o L-Isoleucine L-Leucine L-Lysine x HCl L-Methionine L-Phenylalanine L-Threonine L-Tryptophan L-Tyrosine L-Valine Vitamins D(+)-iotin D-Calcium pantothenate Choline bitartrate 1.80 Choline chloride Folic acid myo-inositol Nicotinamide Pyridoxal x HCl Riboflavin Thiamine x HCl Weight (g / L)

24 DMEM - Media Inorganic salts Other Components Components A1924 A1314 A1925 A1315 A0826 A1926 A1316 A1985 A1986 A1950 A1965 A1979 A1951 A3134 A3137 A3139 Calcium chloride x H 2O Iron(III)-nitrate x H 2O Magnesium sulfate dried Potassium chloride Sodium chloride 64.- Sodium dihydrogen phosphate x H 2O D(+)-Glucose anhydrous HEPES Phenol red Sodium pyruvate Amino acids L-Arginine x HCl L-Cystine L-Glutamine Glycine L-Histidine x HCl x H 20 L-Isoleucine L-Leucine L-Lysine x HCl L-Methionine L-Phenylalanine L-Serine L-Threonine L-Tryptophan L-Tyrosine L-Valine Vitamins D-Calcium pantothenate Choline chloride Folic acid myo-inositol Nicotinamide Pyridoxal x HCl Riboflavin Thiamine x HCl Weight (g / L)

25 DMEM / F12 - Media Components A1920 A1318 A1922 A1317 A1923 A1921 Inorganic salts Other Components Calcium chloride x H 2O Copper(II)-sulfate x5h 2O Iron(II)-sulfate x H2O Iron(III)-nitrate x H 2O Magnesium chloride x 6H 2O Magnesium sulfate dried Potassium chloride Sodium chloride Sodium dihydrogen phosphate x H 2O di-sodium hydrogen phosphate anhydrous Zinc sulfate x 7H 2O D(+)-Glucose anhydrous HEPES Hypoxanthine Linoleic acid DL-alpha-Lipoic acid Phenol red Putrescine x 2HCl Sodium pyruvate Amino acids L-Alanine L-Arginine x HCl L-Asparagine x H2O L-Aspartic acid L-Cysteine x HCl x H 20 L-Cystine L-Glutamic acid anhydrous L-Glutamine Glycine L-Histidine x HCl x H 20 L-Isoleucine L-Leucine L-Lysine x HCl L-Methionine L-Phenylalanine L-Proline L-Serine L-Threonine L-Tryptophan L-Tyrosine L-Valine Vitamins D(+)-iotin D-Calcium pantothenate Choline chloride '-Deoxythymidine Folic acid myo-inositol Nicotinamide Pyridoxal x HCl Pyridoxine HCl Riboflavin Thiamine x HCl Vitamin Weight (g / L)

26 Earle's balanced salts Components A1712 A2039 A3026 A3127 Inorganic salts Calcium chloride x 2H 2O Magnesium sulfate dried Potassium chloride Sodium chloride Sodium dihydrogen phosphate x H2O Other D(+)-Glucose anhydrous Components Phenol red Weight (g / L) Gamborg's 5 Plant salts Components A1911 Inorganic salts Ammonium sulfate 134. oric acid 3. Calcium chloride x 2H 2O Cobalt(II)-chloride x 6H 2O Copper(II)-sulfate x 5H 2O Iron(II)-sulfate x 7H2O Magnesium sulfate dried Manganese(II)-sulfate x H2O 10. Potassium iodide 0.75 Potassium nitrate 25. Sodium dihydrogen phosphate x H 2O 150. Sodium molybdate x 2H 2O 0.25 Zinc sulfate x 7H2O 2. Vitamins D-Calcium pantothenate myo-inositol 1. Nicotinamide 1. Pyridoxal x HCl 1. Riboflavin Thiamine x HCl 10. Weight (g / L)

27 Glasgow - MEM (HK 21) Components A2024 A1320 A2025 A1321 Inorganic salts Calcium chloride x 2H 2O Iron(III)-nitrate x 9H 2O Magnesium sulfate dried Potassium chloride Sodium chloride Sodium dihydrogen phosphate x H2O di-sodium hydrogen phosphate Other D(+)-Glucose anhydrous Components Peptone from casein Peptone from meat Phenol red Yeast extract Amino acids L-Arginine x HCl L-Cystine L-Glutamine L-Histidine x HCl x H 2O L-Isoleucine L-Leucine L-Lysine x HCl L-Methionine L-Phenylalanine L-Threonine L-Tryptophan L-Tyrosine L-Valine Vitamins D-Calcium pantothenate Choline chloride Folic acid myo-inositol Nicotinamide Pyridoxal x HCl Riboflavin Thiamine x HCl Weight (g / L)

28 Grace's - Insect media Components A2023 A1529 Inorganic salts Calcium chloride x 2H 2O Magnesium chloride x 6H 2O Magnesium sulfate dried Potassium chloride di-sodium hydrogen phosphate Other Components D(+)-Glucose anhydrous D(-)-Fructose Fumaric acid a-ketoglutaric acid sodium salt DL-Malic acid Succinic acid D(+)-Sucrose Amino acids L-Alanine L-Arginine x HCl L-Asparagine x H 2O L-Aspartic acid L-Cystine L-Glutamic acid anhydrous L-Glutamine 0 6. Glycine L-Histidine ase L-Isoleucine L-Leucine L-Lysine x HCl L-Methionine L-Phenylalanine L-Proline L-Serine L-Threonine L-Tryptophan L-Tyrosine L-Valine Vitamins p-aminobenzoic acid D(+)-iotin D-Calcium pantothenate Choline chloride Folic acid myo-inositol Nicotinic acid Pyridoxine x HCl Riboflavin Thiamine x HCl Weight (g / L)

29 Ham's F10 - Media Components A1970 A1525 A1989 A1982 A2026 A1952 Inorganic salts Other Components Calcium chloride x 2H 2O Copper(II)-sulfate x5h 2O Iron(II)-sulfate x 7H2O Magnesium sulfate dried Potassium chloride Potassium dihydrogen phosphate Sodium chloride di-sodium hydrogen phosphate Zinc sulfate x 7H 2O D(+)-Glucose anhydrous HEPES '-Deoxythymidine Hypoxanthine DL Phenol red Sodium hydrogen carbonate Sodium pyruvate Amino acids L-Alanine L-Arginine x HCl L-Asparagine x H 2O L-Aspartic acid L-Cysteine x HCl x H2O L-Glutamic acid anhydrous L-Glutamine Glycine L-Histidine x HCl x H2O L-Isoleucine L-Leucine L-Lysine x HCl L-Methionine L-Phenylalanine L-Proline L-Serine L-Threonine L-Tryptophan L-Tyrosine L-Valine Vitamins D(+)-iotin D-Calcium pantothenate Choline chloride Folic acid myo-inositol Nicotinamide Pyridoxine x HCl Riboflavin Thiamine x HCl Vitamin Weight (g / L)

30 Ham's F12 - Media Components A1948 A1526 A1969 A1953 Inorganic salts Calcium chloride x 2H 2O Copper(II)-sulfate x 5H 2O Iron(II)-sulfate x 7H 2O Magnesium chloride x 6H 2O Potassium chloride Sodium chloride di-sodium hydrogen phosphate Zinc sulfate x 7H 2O Other D(+)-Glucose anhydrous Components HEPES '-Deoxythymidine Hypoxanthine Linoleic acid DL-a-Lipoic acid Phenol red Putrescine x 2HCl Sodium pyruvate Amino acids L-Alanine L-Arginine x HCl L-Asparagine x H2O L-Aspartic acid L-Cysteine x HCl x H2O L-Glutamic acid anhydrous L-Glutamine Glycine L-Histidine x HCl x H 2O L-Isoleucine L-Leucine L-Lysine x HCl L-Methionine L-Phenylalanine L-Proline L-Serine L-Threonine L-Tryptophan L-Tyrosine L-Valine Vitamins D(+)-iotin D-Calcium pantothenate Choline chloride Folic acid myo-inositol Nicotinamide Pyridoxine x HCl Riboflavin Thiamine x HCl Vitamin Weight (g / L)

31 Ham's F12K - Media Components A1963 A1927 Inorganic salts Calcium chloride x 2H 2O Copper(II)-sulfate x 5H 2O Iron(II)-sulfate x 7H 2O Magnesium chloride x 6H 2O Magnesium sulfate dried Potassium chloride Potassium dihydrogen phosphate Sodium chloride di-sodium hydrogen phosphate Zinc sulfate x 7H 2O Other Components D(+)-Glucose anhydrous '-Deoxythymidine Hypoxanthine DL-a-Lipoic acid Phenol red Putrescine x 2HCl Sodium pyruvate Amino acids L-Alanine L-Arginine x HCl L-Asparagine x H2O L-Aspartic acid L-Cysteine x HCl x H2O L-Glutamic acid anhydrous L-Glutamine Glycine L-Histidine x HCl x H 2O L-Isoleucine L-Leucine L-Lysine x HCl L-Methionine L-Phenylalanine L-Proline L-Serine L-Threonine L-Tryptophan L-Tyrosine L-Valine Vitamins D(+)-iotin D-Calcium pantothenate Choline chloride Folic acid myo-inositol Nicotinamide Pyridoxine x HCl Riboflavin Thiamine x HCl Vitamin Weight (g / L) Hanks' balanced salts Components A1711 A3140 A2729 A2512 Inorganic salts Calcium chloride x 2H 2O Magnesium sulfate dried Potassium chloride Potassium dihydrogen phosphate Sodium chloride di-sodium hydrogen phosphate anhydrous Other D(+)-Glucose anhydrous Components Phenol red Weight (g / L)

32 IPL-41 - Media Components A2027 A1964 Inorganic salts Ammonium heptamolybdate x 4H 2O Calcium chloride x 2H 2O Cobalt(II)-chloride x 6H 2O Cupric chloride x 6H 2O Iron(II)-sulfate x 7H2O Magnesium sulfate dried Manganese chloride x 4H2O Potassium chloride Sodium chloride Sodium dihydrogen phosphate x H 2O Tin chloride Other Components D(+)-Glucose anhydrous Fumaric acid a-ketoglutaric acid sodium salt DL-Malic acid D(+)-Maltose x H 2O Succinic acid D(+)-Sucrose Amino acids b-alanine L-Arginine x HCl L-Asparagine x H2O L-Aspartic acid L-Cystine L-Glutamic acid anhydrous L-Glutamine Glycine L-Histidine ase L-Hydroxyproline L-Isoleucine L-Leucine L-Lysine x HCl L-Methionine L-Phenylalanine L-Proline L-Serine L-Threonine L-Tryptophan L-Tyrosine L-Valine Vitamins p-aminobenzoic acid D(+)-iotin D-Calcium pantothenate Choline chloride Folic acid myo-inositol Nicotinamide Nicotinic acid Pyridoxine x HCl Riboflavin Thiamine x HCl Vitamin Weight (g / L)

33 Iscove's modified Dulbecco's - Media (IMDM) Components A2028 A1909 A2029 A1322 Inorganic salts Calcium chloride x 2H 2O Magnesium sulfate dried Potassium chloride Potassium nitrate Sodium chloride Sodium dihydrogen phosphate x H2O Sodium selenite anhydrous Other D(+)-Glucose anhydrous Components HEPES Phenol red Sodium pyruvate Amino acids L-Alanine L-Arginine x HCl L-Asparagine x H 2O L-Aspartic acid L-Cystine L-Glutamic acid anhydrous L-Glutamine Glycine L-Histidine x HCl x H2O L-Isoleucine L-Leucine L-Lysine x HCl L-Methionine L-Phenylalanine L-Proline L-Serine L-Threonine L-Tryptophan L-Tyrosine L-Valine Vitamins D(+)-iotin D-Calcium pantothenate Choline chloride Folic acid myo-inositol Nicotinamide Pyridoxal x HCl Riboflavin Thiamine x HCl Vitamin Weight (g / L)

National Nutrient Database for Standard Reference Release 28 slightly revised May, 2016

National Nutrient Database for Standard Reference Release 28 slightly revised May, 2016 National base for Standard Reference Release 28 slightly revised May, 206 Full Report (All s) 005, Cheese, cottage, lowfat, 2% milkfat Report Date: February 23, 208 02:7 EST values and weights are for