ABSTRACT. algicidal bacteria, Alexandrium, Bolinao INTRODUCTION. 1996, Imai et al. 1998, Lovejoy et al Doucette,

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1 Journal of Environmental Science and Management (Special Issue ) ISSN ABSTRACT isolation were collected from 14 selected stations in Bolinao during the Northeast (NE) Monsoon, Tradewind (TW) and species showed more lysis but those in the control wells remained motile and unaffected. Isolated algicidal bacteria can algicidal bacteria, Alexandrium, Bolinao INTRODUCTION Pyrodinium bahamense Alexandrium. San Diego- Prorocentrum minimum Alexandrium.A. minutum A. tamarense Alexandrium. Azanza unpublished Yoshinaga et al. 1995, Fukami et al. 1996, Imai et al. 1998, Lovejoy et al Doucette, McGovern and Babinchak 1999 Alteromonas, Bacillus, Cellulophaga, Cytophaga, Flavobacterium, Micrococcus, Planomicrobium, Pseudoalteromonas, Pseudomonas, Saprospira, Vibrio Alexandrium. Alexandrium 2

2 MATERIALS AND METHOD Figure 1 San Diego- Du et al et al Lovejoy et al Pyrodinium bahamense Alexandrium Alexandrium carterae Gymnodinium catenatum Ostreopsis ovata Azanza-Corrales and Figure 1. Location of sampling stations for marine bacteria in Bolinao, Pangasinan. Alexandrium Alexandrium Lovejoy Isenbarger et al.

3 RESULTS AND DISCUSSION Alexandrium Alexandrium Alexandrium. Alexandrium. Figure 2 Alexandrium Figure 2c P. bahamensefigure 3 G. catenatum Amphidinium carterae Figure 2. Alexandrium sp. strain AMBRVA with Pseudoalteromonas viridis after a) 0 hr, b) 1hr and c) 3 hrs of interaction. Figure 3. Interaction of Bacillus pumilus with P. bahamense var. compressum strain PbcMZRVA a) 0 hr b)1st hr and c) 4th hr. Doucette et al.1998 Doucette et al Cytophaga. Imai et al Cytophaga Karenia brevis Pseudomonas Peridinium bipes

4 Table 1. Initial screening of bacteria isolated from the water column in Bolinao, Pangasinan. Bacteria Vibrio sp. s Gram reaction Catalase + + Vibrio parahaemolyticus + + Vibrio harveyi + + Pseudomonas sp. + + Pseudomonas aeruginosa + + Pseudoalteromonas sp Pseudoalteromonas viridis + + Ruegeria lacuscaerulensis + Roseobacter gallaeciensis + + Pelagibaca sp. + + Ponticoccus sp Alphaproteobacterium Bacillus pumilus + + Microbacterium esteraromaticum Algoriphagus sp. + Coccinimonas marina A. minutum Azam Polz et al. 1999; Furuki and Kobayashi 1991

5 Table 2. Summary of data for marine bacteria active against different microalgae. Bacterial species or strains Algal target(s) Alteromonas spp Doucette et al Kim and Gadd Fukami et al Alexandrium Munn Chatonella antiqua Reference Imai et al Cytophaga Imai et al Cytophaga Karenia brevis Pseudomonas putida Stephanodiscus hantzschii, Microcystis aeruginosa Stephanodiscus hantzschii Saprospira Chaetoceros ceratosporum Micrococcus luteus Cochlodinium polykrikoides Skeletonema costatum Gymnodinium breve Doucette et al Alexandrium tamarense Pseudoalteromonas Cellulophaga lyticaplanomicrobium Bacillus cereus Gymnodinium catenatum StaphylococcusBacillusArthrobacter Pseudoalteromonas Gymnodinum catenatum, Chattonella marina Lovejoy et al Pseudomonas Peridinium bipes AlteromonasIdiomarina Alexandrium tamarense R. lacuscaerulensis Alexandrium Ruegeria Roseobacter Ruegeria Phaeobacter Vibrio anguillarum in vitro R. lacuscaerulensis Kolber Ruegeria lacuscaerulensisroseobacter gallaeciensis Pelagibaca Ponticoccus Pseudoalteromonas V. harveyi VibrioV. parahaemolyticus Pseudomonas Pseudomonas aeruginosa

6 Pseudomonas viridis Alexandrium Vibrio Lee Baffone Roszak and Colwell 1987V. parahaemolyticus Liston 1990 Cochlodinium polykrikoides ymnodinium impudicumprorocentrum minimumprorocentrum micansamphidinium carterae V. harveyi Penaeus monodonlavilla-pitogo et al Paralichthys olivaceus Sebastes schlegeli V. parahaemolyticus V. harveyialexandrium Pseudomonas Pseudomonas Pseudomonas Chaetoceros ceratosporum Stephanodiscus hantzschiipseudomonas Kodani et al. P. aeruginosa Ren et al.p. aeruginosa Microcystis aeruginosa P. aeruginosa Wang et al. P. aeruginosaalexandrium Pseudoalteromonas Pseudoalteromonas Alexandrium tamarensepseudoalteromonas haloplanktis P. minimumkim et al. P. viridislee unpublishedp. viridis Alexandrium Pseudoalteromonas Class Bacilli Bacillus B. marinusb. salexigensb. dipsosauri Fusetani et al. 1987Bacillus B. marinusb. badiusb. subtilisb. cereusb. licheniformisb. lentus Boeye and Aerts 1976; Ortigosa et al Bacillus Chatonella marinakim et B. cereus B. pumilus Gelliodes carnosa Alexandrium Kocher et Microbacterium esteraromaticum Cytophaga FlavobacteriaBacteroidesCytophaga Algoriphagus Alegado et al Coccinimonas marina KirchmanAlgoriphagus Alexandrium Genus Algoriphagus A. ratkowskyi Algoriphagus Tao et Coccinimonas marina

7 Cottrell and Algoriphagus C. marina CONCLUSION AND RECOMMENDATIONS Alexandrium REFERENCES Microbacterium Gymnodinium breve Vibrio parahaemolyticus Pyrodinium bahamense compressum Prorocentrum minimum Vibrio Gymnodinium nagasakiense Skeletonema costatum

8 circularisquama Stephanodiscus hantzschii Pseudomonas Stephanodiscus hantzschii Stephanodiscus hantzschii Cochlodinium polykrikoides Prorocentrum minimum Pseudoalteromonas haloplanktis Chattonella marina Pseudomonas aeruginosa Pseudomonas

9 Gelliodes carnosa Algoriphagus aquatilis Chattonella Gymnodinium Agrobacterium ferrugineum Agrobacterium gelatinovorum Agrobacterium stellulatum Pseudomonas Scophthalmus maximus Sporosarcina halophilia halophilus Alexandrium tamarense Alexandrium tamarense

10 Algoriphagus Algoriphagus antarcticus Pseudomonas aeruginosa Vibrio harveyi salinus Gymnodinium mikimotoi Bacillus cereus