CASCONE PASQUALE

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1 PERSONAL INFORMATION CASCONE Pasquale Sex Male Date of birth 5 DIC. 80 Nationality Italian POSITION HELD Researcher at Italian National Research Council (CNR) WORK EXPERIENCE 10 OCT 2012 TODAY Researcher, 15 JUL OCT 2012 Postdoc Institute for Sustainable Plant Protection - Italian National Research Council (CNR) Università 133, Portici (Italia) Joining the study project concerns the sustainable control of Asian gall wasp of chestnut (Dryocosmus kuriphilus) founded by Regione Campania under the scientific coordination of professor Emilio Guerrieri. The main goal is: The improving of Torymus sinensis massal rearing to control Asian gall wasp of chestnut Braconid wasp parasitoids attractiveness assay in a controlled environment on tomato, bean, tobacco, potato and brassica plants Understanding signaling processes involved in plant defence against insects, including: rhizosphere plant-plant signaling, arthropod-derived elicitors and priming Study of population dynamics of the Dryocosmus kuriphilus through monitoring by sticky traps and microscopic observations of different morphological stages Phylogenetics and biosystematics characterization of parasitoid wasps (Hymenoptera: Chalcidoidea) potentially useful in biological control programmes Integrative taxonomy (morphological, biological and molecular) conducted on parasitoids of Dryocosmus kuriphilus Yasumatsu using: DNA barcoding, amplification of conserved nuclear and mitochondrial regions, and morphometric analysis Development of specific molecular markers as RFLP-PCR to rapidly identify parasitoid and their hosts Use of parasitoids in an environmentally-friendly manner to control invasive exotic insects and pests damaging greenhouse crops Evaluation of chemical natural products on aphids behavioural and fitness Joining as collaborator the project: PURE - Pesticide Use-and-risk Reduction in European farming systems with Integrated Pest Management. Evaluating field releasing of T. absoluta natural enemies through an integrative taxonomy approach (using dichotomous morphologic keys and molecular techniques) Evaluating the performance of T. absoluta natural enemies through lab and field bioassays concern: parasitization, development, longevity and fertility. Business or sector Systematic biology, Integrated pest management, Entomology Institute for Sustainable Plant Protection - Italian National Research Council (CNR) Università 133, Portici (Italia) Joining the study projects concern the sustainable control of Asian gall wasp of chestnut (Dryocosmus kuriphilus) founded by Regione Campania under the scientific coordination of professor Emilio Guerrieri. Projects: Volatiles organic compounds involved in the attractiveness of Asian gall wasp of chestnut (Dryocosmus kuriphilus)" "Collecting and characterization of Dryocosmus kuriphilus natural Braconid wasp parasitoids attractiveness assay in a controlled environment on tomato, bean, tobacco, potato and brassica plants Page 1 / 6

2 Understanding signaling processes involved in plant defence against insect, including: rhizosphere plant-plant signaling, arthropod-derived elicitors and priming Study of population dynamics of the Dryocosmus kuriphilus through monitoring by sticky traps and microscopic observations of different morphological stages Phylogenetics and biosystematics characterization of parasitoid wasps (Hymenoptera: Chalcidoidea) potentially useful in biological control programmes Integrative taxonomy (morphological, biological and molecular) conducted on parasitoids of Dryocosmus kuriphilus Yasumatsu using: DNA barcoding, amplification of conserved nuclear and mitochondrial regions, and morphometric analysis Development of specific molecular markers as RFLP-PCR to rapidly identify parasitoid and their hosts Use of parasitoids in an environmentally-friendly manner to control invasive exotic insects and pests damaging greenhouse crops Business or sector Systematic biology, Integrated pest management, Entomology 16 JUN JUL 2014 Visiting Researcher Working on Diaretiella rapae attractiveness bioassays (y-tube and wind-tunnel) on Brassica nigra plants exposed to different biotic stresses such as aphids and chewers and their 2 MAR MAR 2014 Visiting Researcher Working on Diaretiella rapae attractiveness biossays (y-tube and wind-tunnel) on Brassica nigra plants exposed to differet biotic stresses such as aphids and chewers and their 18 MAR JUN 2013 Visiting Researcher Working on Diaretiella rapae attractiveness biossays (y-tube and wind-tunnel) on Brassica nigra plants exposed to differet biotic stresses such as aphids and chewers and their 14 OCT NOV 2012 Visiting Researcher Working on Diaretiella rapae attractiveness biossays (y-tube and wind-tunnel) on Brassica Page 2 / 6

3 nigra plants exposed to differet biotic stresses such as aphids and chewers and their 1 OCT NOV 2010 Visiting Researcher Rothamsted Research Experimental Station Herpenden, AL5 2JQ (Regno Unito) Joining the project: Elucidating the chemical ecology of belowground plant-plant communication project. Performing characterization of root exudates andvolatile substances emitted by tomato and beans plants by gas chromatographyand mass spectrometry. The project was supervised by Michael Birkett. 1 FEB JUN 2010 Teacher at high school institute Istituto d'istruzione Superiore Statale Alessandro Manzoni via Morselli 10, Varese (Italia) Teaching Natural Science, Chemistry, Astronomy and Microbiology Business or sector Education 1 NOV DIC 2009 Postdoc Università degli studi di Napoli "Federico II" dipartimento DISPAPA Via Università 100, Portici (Na) (Italia) Joining the projects: GenoPOM - Supervision and experimentation on insect behaviour in artificial laboratory conditions on tomato plants GenoPOM - multitrophic study of interactions between tomato plants and biotic factors". under the scientific coordination of professor Luigi Frusciante Analysis of gene expression by Northern blot analysis with radioactive probes, RT-Realtime PCR with SYBR green chemistry and Taqman probes, and semiquantitative PCR on agarose gel Cloning of genes into vectors for sequencing using Echerichia coli Expression of genes under thecontrol of inducible promoters and constitutive in plant by stable and transient transformation Genetic nuclear transformation by Agrobacterium tumefaciens and by Gene gun of Solanaceae Protein Extraction and analysis by western blot Bioassays with insects in artificial conditions such as aphids, moths, wasps on tomato plants Bioassays in artificial conditions with biotrophic and necrotrophic fungi on Solanaceae Gene expression under the control of constitutive and inducible promotors in plant. Business or sector Molecular biology, Plant Genetics, In vitro Culture, Plant Biotechnology EDUCATION AND TRAINING 1 NOV JUL 2008 DEGREE in Plant Biotechnology ISCED 5 Università degli Studi di Napoli "Federico II" Thesis title: Study of the role of sesquiterpene synthase in tomato indirect defense against aphids. Grade: 110/110 Page 3 / 6

4 1 NOV FEB 2008 DOCTOR OF PHILOSOPHY in Insect Biotechnology ISCED 6 Università della Basilicata Thesis title: Study of defense genes involved in the production of volatile organic compounds in tomato 4 SEP OCT 2007 Stage Rothamsted Research - Department of Biological Chemistry Learning techniques: gas chromatography, mass spectrometry, insect behaviour bioassays, collection of volatiles by air entrainment 19 OCT MAR 2007 Stage Metapontum Agrobios s.r.l. Learning of techniques: Plant Genetic Transformation, Plant Propagation by Tissue Culture 1 NOV OCT 2004 DEGREE in Agribusiness Biotechnology ISCED 5 Università degli studi di Napoli "Federico II" PERSONAL SKILLS Thesis title: The role of prosistemin in the induction of genes involved in direct and indirect defense against aphids, Grade: 110/110 Mother tongue(s) Italian Other language(s) UNDERSTANDING SPEAKING WRITING Listening Reading Spoken interaction Spoken production English C2 C2 C2 C2 C2 Livelli: A1/A2: Livello base - B1/B2: Livello intermedio - C1/C2: Livello avanzato Quadro Comune Europeo di Riferimento delle Lingue Organisational / managerial skills Good laboratory experience, tutor of doctoral students and undergraduates, good sense of organization, strong organization skills and inter-personal, excellent ability to learn Computer skills Driving licence Good command of Microsoft Office tools (Word, Excel and PowerPoint ) and of Adobe graphic design application (Adobe Creative Suite), good command of Vector NTI(Invitrogen), Clone Manager (Sci-Ed Software), SDS e RQ manager (Applied),Image Quant (Molecular Dynamics), Sigma Plot e Systat (Systat Software), Endnote (Thomson Reuters) and Mega 5 (Koichiro Tamura et al) Advanced knowledge of HTML language and Microsoft Visual Basic Linux Exepert User (Xubuntu, Fedora, Debian) Excellent knowledge of: Microsoft operating systems since MS-DOS to Windows 7, Apple MacOS X and Classic 9 EIPASS 7 modules certification AM, A1, A2, B PUBLICATIONS Cascone, P., L. Iodice, et al. (2015). "Tobacco overexpressing< i> β</i>-ocimene induces direct and indirect responses against aphids in receiver tomato plants." Journal of plant physiology 173: Coppola, M., G. Corrado, et al. (2014). "Prosystemin Overexpression in Tomato Enhances Resistance to Different Biotic Stresses by Activating Genes of Multiple Signaling Page 4 / 6

5 CONFERENCE Pathways." Plant Molecular Biology Reporter: Palmeri, V., P. Cascone, et al. (2014). "Hymenoptera wasps associated with the Asian gall wasp of chestnut (Dryocosmus kuriphilus) in Calabria, Italy." Phytoparasitica: 1-4. Panzavolta, T., U. Bernardo, et al. (2013). "Native parasitoids associated with Dryocosmus kuriphilus in Tuscany, Italy." Bulletin of Insectology 66(2): Battaglia, D., S. Bossi, et al. (2013). "Tomato Below Ground-Above Ground Interactions: Trichoderma longibrachiatum Affects the Performance of Macrosiphum euphorbiae and Its Natural Antagonists (vol 26, pg 1249, 2013)." MOLECULAR PLANT- MICROBE INTERACTIONS 26(12): Bernardo, U., L. Iodice, et al. (2013). "Biology and monitoring of Dryocosmus kuriphilus on Castanea sativa in Southern Italy." Agricultural and Forest Entomology 15(1): Digilio, M. C., P. Cascone, et al. (2012). "Interactions between tomato volatile organic compounds and aphid behaviour." Journal of Plant Interactions 7(4): Guerrieri, E., U. Bernardo, et al. (2012). "Gestire il cinipide del castagno mediante i nemici naturali." L'Informatore agrario 6: Orsini, F., P. Cascone, et al. (2010). "Systemin dependent salinity tolerance in tomato: evidence of specific convergence of abiotic and biotic stress responses." Physiologia plantarum 138(1): Paper awarded as the best scientific publication produced by young researchers in the field of horticulture for year Corrado, G., R. Sasso, et al. (2007). "Systemin regulates both systemic and volatile signaling in tomato plants." Journal of Chemical Ecology 33(4): Grasso F, Paduano A, Genovese A, Cascone P, Guerrieri E, Rao R, Sacchi S. Olive volatile compounds and their variations induced by the olive fly attack. III Convegno Nazionale dell Olivo e dell Olio, Bari 26-28/11/2014 Palmeri V, Campolo O, Grande SB, Laudani F, Malacrinò A, Guerrieri E, Cascone P. Imenotteri parassitoidi indigeni associati a Dryocosmus kuriphilus Yasumatsu in Calabria. CASTANEA 2014 Viterbo 22-25/09/2014 Cascone P, Iodice L, Carpenito S, Ferracini C, Alma A, Guerrieri E. Ottimizzazione della produzione massale di Torymus sinensis per il controllo del cinipide galligeno del castagno (Progetto OPTYMUS - Piano di Sviluppo Rurale Regione Campania). CASTANEA 2014 Viterbo 22-25/09/2014 Ponzio C, Cascone P, Cusumano A, Weldegergis BT, Fatouros NE, Guerrieri E, Dicke M, Gols R. Foraging behavior of three parasitoid species during dualherbivore attack to plants; does herbivore density matter?. 15th International Symposium on Insect-Plant Relationships, University of Neuchâtel 17-22/08/2014 Cascone P, Ponzio C, Gols R, Dicke M, Guerrieri E. Does Non-Host Damage Affect Diaeretiella Rapae Behaviour Towards Hipv From Brassica Nigra. 15th International Symposium on Insect-Plant Relationships, University of Neuchâtel 17-22/08/2014 Digilio MC, Mezioud D, Cascone P, Iodice L, Ruocco M, Lorito M, Scala F, Guerrieri E, Pennacchio F. Effetto di Trichoderma spp sulla modulazione della difesa diretta ed indiretta in pomodoro. XXIV Congresso Nazionale Italiano di Entomologia, Orosei 9-14/06/2014, ISBN: Loru L, Cascone P, Fadda ML, Fois X, Guerrieri E, Pantaleoni RA. Dryocosmus kuriphilus (Hymenoptera Cynipidae) in Sardegna. XXIV Congresso Nazionale Italiano di Entomologia, Orosei 9-14/06/2014, ISBN: Ruocco M, Lombardi N, Lanzuise S, Guerrieri E, Cascone P, Iodice L, Woo S, Vinale F, Marra R, Pascale A, Manganiello G, Varlese R, Matteoli V, Scala F and Lorito M. Trichoderma longibrachiatum affects released vocs (volatile organic compounds) profile and tomato-insect interaction. XIX Convegno della Socità Italiana di Patologia Vegetale, Padova 23-25/09/2013 Ruocco M, Bernardo U, Guerrieri E, Giorgini M, Cascone P, Marra R, Lanzuise S, Woo S, Lombardi N, Manganiello G, Varlese R, Pascale A, Mazzei P, Piccolo A, Caira S and Lorito M Metabolites Produced By Gnomoniopsis Castanea Associated With Necrosis Of Chestnut Galls. XIX Convegno della Socità Italiana di Patologia Vegetale, Padova 23-25/09/2013 Guerrieri E, Cascone P, Iodice I, Digilio MC. Interactions Between Tomato Volatile Organic Compounds And Aphid Behaviour. XXIV International Congress of Entomology /08/2012 EXCO, Daegu, Republic of Korea Guerrieri E, Cascone P, Iodice I, Dewhirst SY, Martin J, Pickett JA, Birkett MA. Elucidating The Chemical Ecology Of Belowground Plant-Plant Communication. XXIV International Congress of Entomology /08/2012 EXCO, Daegu, Republic of Korea Dewhirst SY, Cascone P2, Lodice L2, Pickett JA, Guerrieri E, Birkett MA. Elucidating the chemical ecology of belowground plant-plant communication. Gordon research Conferences 2012 Plant Volatiles: Ecology, Biosynthesis, Regulation and Animal Perception of Floral and Vegetative Volatiles, Plus their Roles in Human Flavor and Agriculture January 29/01-03/02/2012 Ventura Beach Marriott Ventura, CA Page 5 / 6

6 Calafiore R, Ruocco M, Lorito M, Cascone P, Rao R. Expression Of Trichoderma Harzianumm Hydrophobin In Solanum Lycopersicum. Proceedings of the Joint Meeting AGI-SIBV- SIGA Assisi, 19-22/09/2011, ISBN Bernardo U, Iodice L, Sasso R, Gebiola M, Cascone P, Guerrieri E. Biologia, monitoraggio e controllo di dryocosmus kuriphilus in Campania. XXIII Congresso Nazionale Italiano di Entomologia Genova, 13-16/06/2011, ISBN Digilio MC, Iodice L, Cascone P, Guerrieri E. Interazione tra composti volatili prodotti dal pomodoro e comportamento degli afidi. XXIII Congresso Nazionale Italiano di Entomologia Genova, 13-16/06/2011, ISBN Coppola M, Cascone P, Digilio MC, Ruocco M, Rao R. L espressione costitutiva della prosistemina come strumento per la difesa del pomodoro. XXII Congresso Nazionale Italiano di Entomologia Ancona 15-18/06/2009, ISBN Coppola M, Cascone P, Rao R. L espressione costitutiva della prosistemina come strumento per la difesa del pomodoro. Giornate Scientifiche Portici, Italy, 10-12/12/2008 Cascone P, Coppola M, Rao R. Tomato sesquiterpene synthase SSTLe1-2 and GCS mediate tritrophic interactions. SIGA Annual Congress Padova, Italy, 14-17/09/2008, ISBN Poster premiato dalla Società Italiana di Genetica Agraria come miglior contributo presentato alla conferenza 2008 Cascone P, Coppola M, Schettino M, Rao R. Prosystemin over-expression in different tomato genetic backgrounds. SIGA Annual Congress Padova, Italy, 14-17/09/2008, ISBN Cascone P. Study of tomato defence genes involved in the production of Volatile Organic Compounds. Young Ideas in Insect Science, Meeting of PhD Students and Post- Doctoral Fellows Firenze, Italy, 5-6/06/2008 Cascone P, Corrado G, Coppola M, Rao R. The constitutive production of systemin in tomato plants modifies the expression of genes involved in the VOC pathway. SIGA Annual Congress Riva del Garda, Italy, 23-26/09/2007, ISBN Orsini F, Cascone P, Bottino A, Maggio A, Corrado G, De Pascale S, Rao R. The overexpression of systemin in tomato improves the agronomic performance of plants grown under salt stress conditions. SIGA Annual Congress Riva del Garda, Italy, 23-26/09/2007, ISBN Orsini F, Cascone P, Bottino A, Maggio A, Corrado G, De Pascale S, Rao R. Effetti della sistemina sulle relazioni idriche e la crescita in piante di pomodoro esposte a stress salino. Giornate Scientifiche Napoli, Italy, 20-21/08/2007 Corrado G, Guerrieri E, Pasquariello M, Digilio MC, Cascone P, Rao R. Systemin regulates volatile signalling in tomato Plant & Animal Genome XV. Conference San Diego, United States, 13-17/01/ 2007 Pasquariello M, Digilio MC, Rao R, Guerrieri E, Cascone P, Corrado G. Plant response to herbivorous and phloem-feeder pests: a study of defense genes in tomato. SIGA Annual Congress Ischia, Italy, 10-14/09/2006, ISBN Cascone P, Corrado G, Pasquariello M, Melchiade D, Digilio M C, Guerrieri E, Rao R. Effect of Systemin on the Tri-trophic Interaction between Tomato Plants, Aphids and Parasitic Wasps. 2nd Solanaceae Genome Workshop Ischia, Italy, 25-29/09/2005 Page 6 / 6

Curriculum Vitae. Grazia Luparello

Curriculum Vitae. Grazia Luparello Curriculum Vitae Grazia Luparello Personal Data: Surname Luparello Name Grazia Address via Portofino 30/42, 10135 Torino Telephone +39 0113910032 Mobile +39 3920182152 E-mail luparell@to.infn.it Nationality