Curriculum Vitae of: Hendrik Johannes Niemann

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1 Curriculum Vitae of: Hendrik Johannes Niemann

2 Address: 20 Maine ave., Roodekrans, Roodepoort South Africa Tel: PROFILE A diligent student researcher currently working on the development of a DNA barcoding based identification protocol to aid in the fight against invasive aquatic plants in South Africa. With a keen interest in conservation biology and the preservation of indigenous ethnobotanical knowledge, I strive to learn as much as possible so that I may contribute towards these passions and the scientific community as a whole in a meaningful way. KEY ACHIEVEMENTS ü 2010: Obtained a National Senior Certificate with distinctions in Afrikaans Home Language, English First Additional Language, Geography, Mathematics, Life Sciences and Life Orientation. ü 2016: Was awarded an NRF free standing bursary for Honours studies ü 2016: Obtained BSc. Honours degree in Botany and Plant Biotechnology with distinction (cum laude) ü 2017: UJ academic merit bursary holder for Masters studies EMPLOYMENT HISTORY African Centre for DNA Barcoding (ACDB) University of Johannesburg University of Johannesburg Demonstrator/Invigilator African Centre for DNA Barcoding (ACDB) 2013 December December Present 2017 Present Extraction and isolation of DNA from plant material. Amplification and purification of DNA for sequencing purposes. Extraction and isolation of DNA and RNA from plant material. Amplification and purification of nucleic acids for sequencing and gene expression studies. Training of students in practical aspects of botany. Invigilating student tests and examinations.grading bodies of work. Extraction and isolation of DNA from plant material. Amplification and purification of DNA for sequencing purposes.dna sequencing, barcoding and phylogenetic analysis. Page 2 of 5

3 EDUCATION AND TRAINING Subjects: BSc with specialisation in Biochemistry & Botany ( 2011 December 2015) Biochemistry 1, 2 & 3 (A & B) Biology 1A Botany 1, 2 & 3 (A & B) Chemistry 1 (A & B) Computer Competence 1 Mathematics 1 (A & B) Physics 1A Subjects: BSc Honours with specialisation Botany & Plant Biotechnology (cum laude) ( 2016 December 2016) Plant Biotechnology Plant Systematics and Molecular Evolution Advanced Plant Taxonomy and Economic Botany Post-Harvest Physiology Scientific Methods Thesis: A Review of the Taxonomy, Ethnobotany, and Chemistry of Pelargonium luridum (Andr.)Sweet and Pelargonium triste (L.) L herit. MSc with specialisation in Molecular Systematics and DNA Barcoding ( 2017 present) CORE SKILLS IT Proficiency: MS Word, Outlook, Excel, Powerpoint, Paup, Sequencher, Mesquite and Geneious. Page 3 of 5

4 DNA Barcoding: Basic DNA extraction, Gene amplification via PCR (polymerase chain reaction). Cycle Sequencing of gene regions. Editing and aligning of DNA sequences. Database comparisons of DNA sequences and compilation of phylogenetic trees. Phytochemistry: Extraction, isolation and identification of phytochemicals from plant material using chromatographic and spectroscopic techniques (Column chromatography, HPLC, LC-MS, NMR).Testing for bioactivity with skills in antimicrobial assays (MIC). Languages: English (Fluent) Afrikaans (Fluent) SCIENTIFIC CONFERENCES , Post-graduate symposium Title: A comparative phytochemical investigation of Pelargonium sidoides DC. and Pelargonium luridum (Andr.) Sweet (Geraniaceae) oral and poster presentation. University of the Western Cape, SAAB Annual Congress Title: Phytochemical profiling of some red rooted species of Pelargonium (Geraniaceae) with a documented history of ethnomedicinal use oral presentation. African Centre for DNA barcoding (ACDB), 7 th International Barcode of Life (ibol) conference Title: A DNA barcoding approach to assess the risk posed by the aquarium trade in the spread of invasive aquatic plants in South Africa oral presentation. REFERENCES Prof. Michelle van der Bank African Center for DNA Barcoding Director and Masters supervisor mvdbank@uj.ac.za Tel: Dr. Nicholas J. Sadgrove Post-Doctoral research fellow and Honours supervisor nicholas.sadgrove@gmail.com Tel: Page 4 of 5

5 Prof. Annah Moteetee Head of Department; Botany and Plant Biotechnology Tel: Dr. Bezeng S. Bezeng African Center for DNA Barcoding Post-Doctoral research fellow and Masters co-supervisor Tel: Page 5 of 5

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