Supplementary Information

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1 Supplementary Information Systematic characterization of the gene family provides new insights into fungal pathogenicity in Magnaporthe oryzae Albely Afifa Mir 1, Sook-Young Park 1, 2, Md. Abu Sadat 1,3, Seongbeom Kim 1, Jaeyoung Choi 1,4, Junhyun Jeon 1,5, and Yong-Hwan Lee 1 1 Department of Agricultural Biotechnology, Fungal Bioinformatics Laboratory, Center for Fungal Genetic Resources, and Center for Fungal Pathogenesis, Seoul National University, Seoul 151-1, Korea 2 Current Address: Korean Lichen Research Institute, Sunchon National University, Sunchon , Korea 3 Current address: Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 406-7, Korea 4 Current Address: Department of Forest Sciences, University of Helsinki, Helsinki, Finland 5 Current Address: School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk , Korea Corresponding author: Yong-Hwan Lee * yonglee@snu.ac.kr. Telephone:

2 MGG 08256T0 (MoPRX1) Magnaporthe oryzae -15 GLRG 0T0 Colletotrichum graminicola M1.001 NCU031T0 Neurospora crassa Pa 5 46 Podospora anserina FGSG 036T0 Fusarium graminearum FOXG 15430T0 Fusarium oxysporum f. sp. lycopersici BC1G Botrytis cinerea estext Genewise1Plus.C chr Mycosphaerella graminicola bgh02344 polypeptide Blumeria graminis f. sp. hordei DH14 ANID Aspergillus nidulans FGSC A4 Afu4g080 Aspergillus fumigatus Af2 HCEG Histoplasma capsulatum H88 CIMG Coccidioides immitis estext fgenesh1 pm.c chr Mycosphaerella graminicola ANID 033 Aspergillus nidulans FGSC A4 Afu8g07130 Aspergillus fumigatus Af2 BC1G Botrytis cinerea PGTG 142 Puccinia graminis f. sp. tritici EuGene.002 Melampsora larici-populina AG31 UM Ustilago maydis 1 fgenesh1 pm.c scaffold Phanerochaete chrysosporium estext Genewise1 human.c Laccaria bicolor fgenesh1 kg.15 # 565 # CFOT CFPN Serpula lacrymans S7.3 Hetan1.estExt fgenesh3 kg.c Heterobasidion irregulare TC 32-1 fgeneshpb pm.2 Phycomyces blakesleeanus fgeneshpb pm.73 4 Phycomyces blakesleeanus RO3G Rhizopus oryzae CNAG Cryptococcus neoformans serotype A estext Genewise1Plus.C 0001 Batrachochytrium dendrobatidis JAM81 orf Candida albicans SC5314 YBL064C Saccharomyces cerevisiae S288C Afu5g150 Aspergillus fumigatus Af2 e gwh Phanerochaete chrysosporium AT1G Arabidopsis thaliana LOC Os07g Oryza sativa LOC Os07g Oryza sativa FBpp Drosophila melanogaster ENSP Homo sapiens FBpp Drosophila melanogaster FBpp Drosophila melanogaster FBpp Drosophila melanogaster Y38C1AA.11 Caenorhabditis elegans FGSG 03180T0 Fusarium graminearum FOXG 10111T0 Fusarium oxysporum f. sp. lycopersici GLRG 10121T0 Colletotrichum graminicola M1.001 estext fgenesh1 pg.c 169 Serpula lacrymans S7.3 estext Genewise1 human.c 2000 Laccaria bicolor fgenesh1 pg.c scaffold Phanerochaete chrysosporium estext fgenesh1 pm.c 148 Heterobasidion irregulare TC 32-1 estext Genewise1.C 207 Phycomyces blakesleeanus RO3G Rhizopus oryzae RO3G Rhizopus oryzae RO3G Rhizopus oryzae AMAG 01034T0 Allomyces macrogynus AMAG 02542T0 Allomyces macrogynus PITG 154 Phytophthora infestans FBpp Drosophila melanogaster FBpp00735 Drosophila melanogaster F09E5.15a Caenorhabditis elegans F09E5.15b Caenorhabditis elegans ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens PITG Phytophthora infestans FBpp002 Drosophila melanogaster FBpp003 Drosophila melanogaster YDR453C Saccharomyces cerevisiae S288C YML028W Saccharomyces cerevisiae S288C orf Candida albicans SC5314 orf Candida albicans SC5314 SPCC5.03c Schizosaccharomyces pombe R07E5.2 Caenorhabditis elegans ENSP Homo sapiens ENSP Homo sapiens estext Genewise1Plus.C 058 Batrachochytrium dendrobatidis JAM81 FBpp00827 Drosophila melanogaster FBpp003 Drosophila melanogaster PITG 000 Phytophthora infestans GP Batrachochytrium dendrobatidis JAM81 AMAG 009T0 Allomyces macrogynus AMAG 0T0 Allomyces macrogynus estext Genewise1.C 1 Phycomyces blakesleeanus RO3G 153 Rhizopus oryzae AT3G Arabidopsis thaliana AT5G062.1 Arabidopsis thaliana LOC Os04g330.1 Oryza sativa LOC Os02g Oryza sativa LOC Os02g Oryza sativa CNAG Cryptococcus neoformans serotype A PITG 006 Phytophthora infestans ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens CAD Encephalitozoon cuniculi ENSP Homo sapiens LOC Os07g Oryza sativa ENSP Homo sapiens MGG 003T0 (TPX1 ) Magnaporthe oryzae -15 FGSG 102T0 Fusarium graminearum FOXG 05110T0 Fusarium oxysporum f. sp. lycopersici GLRG 07483T0 Colletotrichum graminicola M1.001 ANID Aspergillus nidulans FGSC A4 BC1G 040 Botrytis cinerea estext fgenesh1 kg.c chr 1201 Mycosphaerella graminicola NCU11657T0 Neurospora crassa CIMG Coccidioides immitis HCEG Histoplasma capsulatum H88 bgh042 polypeptide Blumeria graminis f. sp. hordei DH14 Afu4g05910 Aspergillus fumigatus Af2 PGTG Puccinia graminis f. sp. tritici fgenesh2 pg.25 # 19 Melampsora larici-populina AG31 UM041.1 Ustilago maydis 1 e gwh Phanerochaete chrysosporium estext Genewise1 worm.c Laccaria bicolor estext fgenesh1 kg.c 1504 Serpula lacrymans S7.3 Hetan1.fgenesh2 pg.c scaffold Heterobasidion irregulare TC 32-1 CNAG 024 Cryptococcus neoformans serotype A AMAG 016T0 Allomyces macrogynus AMAG 12030T0 Allomyces macrogynus YIL010W Saccharomyces cerevisiae S288C orf Candida albicans SC5314 SPBC c Schizosaccharomyces pombe PITG 005 Phytophthora infestans PITG 205 Phytophthora infestans UM Ustilago maydis 1 fgenesh1 pg.c scaffold Phanerochaete chrysosporium estext fgenesh1 kg.c Serpula lacrymans S7.3 eu2.lbscf0003g010 Laccaria bicolor Hetan1.estExt Genewise1Plus.C 718 Heterobasidion irregulare TC 32-1 gw Phycomyces blakesleeanus AT3G2.1 Arabidopsis thaliana AT3G2.2 Arabidopsis thaliana LOC Os06g010.1 Oryza sativa LOC Os06g010.2 Oryza sativa Afu5g02300 Aspergillus fumigatus Af2 MGG 04337T0 (CPXA) Magnaporthe oryzae -15 Pa Podospora anserina NCU057T0 Neurospora crassa GLRG 05821T0 Colletotrichum graminicola M1.001 Afu8g016 Aspergillus fumigatus Af2 bgh05777 mrna polypeptide Blumeria graminis f. sp. hordei DH14 ANID Aspergillus nidulans FGSC A4 estext fgenesh1 kg.c chr 80 Mycosphaerella graminicola FGSG 024T0 Fusarium graminearum FOXG 122T0 Fusarium oxysporum f. sp. lycopersici UM033.1 Ustilago maydis 1 MGG 034T0 (CPXB) Magnaporthe oryzae -15 GLRG 026T0 Colletotrichum graminicola M1.001 FGSG 12369T0 Fusarium graminearum FOXG 14234T0 Fusarium oxysporum f. sp. lycopersici FOXG 17180T0 Fusarium oxysporum f. sp. lycopersici FOXG 17106T0 Fusarium oxysporum f. sp. lycopersici FOXG 17130T0 Fusarium oxysporum f. sp. lycopersici estext Genewise1Plus.C chr Mycosphaerella graminicola PITG 055 Phytophthora infestans PITG 055 Phytophthora infestans PITG Phytophthora infestans MGG 04545T0 (MoCCP1) Magnaporthe oryzae -15 FGSG 01245T0 Fusarium graminearum FOXG 00142T0 Fusarium oxysporum f. sp. lycopersici GLRG 04508T0 Colletotrichum graminicola M1.001 NCU032T0 Neurospora crassa NCU032T1 Neurospora crassa Pa 1 Podospora anserina BC1G Botrytis cinerea bgh00659 polypeptide Blumeria graminis f. sp. hordei DH14 ANID Aspergillus nidulans FGSC A4 Afu4g09110 Aspergillus fumigatus Af2 HCEG Histoplasma capsulatum H88 CIMG Coccidioides immitis UM Ustilago maydis 1 e gw Batrachochytrium dendrobatidis JAM81 e gw Phycomyces blakesleeanus RO3G Rhizopus oryzae e gwh Phanerochaete chrysosporium Hetan1.EuGene Heterobasidion irregulare TC 32-1 estext fgenesh1 kg.c 177 Serpula lacrymans S7.3 gww Laccaria bicolor CNAG Cryptococcus neoformans serotype A MGG 10368T0 (MoCCP2) Magnaporthe oryzae -15 FGSG 106T0 Fusarium graminearum FOXG 13439T0 Fusarium oxysporum f. sp. lycopersici BC1G 111 Botrytis cinerea bgh023 polypeptide Blumeria graminis f. sp. hordei DH14 ANID Aspergillus nidulans FGSC A4 Afu6g135 Aspergillus fumigatus Af2 HCEG Histoplasma capsulatum H88 CIMG Coccidioides immitis PGTG Puccinia graminis f. sp. tritici estext fgenesh1 pg.c Melampsora larici-populina AG31 UM Ustilago maydis 1 e gw Batrachochytrium dendrobatidis JAM81 AMAG 09111T0 Allomyces macrogynus AMAG 113T0 Allomyces macrogynus estext Genewise1.C Phycomyces blakesleeanus RO3G 054 Rhizopus oryzae AMAG 081T0 Allomyces macrogynus AMAG 12087T0 Allomyces macrogynus CNAG Cryptococcus neoformans serotype A PGTG 165 Puccinia graminis f. sp. tritici estext Genewise1Plus.C Melampsora larici-populina AG31 orf19.5 Candida albicans SC5314 YKR0C Saccharomyces cerevisiae S288C orf Candida albicans SC5314 PITG 004 Phytophthora infestans AT1G0.1 Arabidopsis thaliana AT1G0.2 Arabidopsis thaliana AT1G0.3 Arabidopsis thaliana AT1G0.4 Arabidopsis thaliana AT1G0.5 Arabidopsis thaliana AT1G0.7 Arabidopsis thaliana AT1G0.8 Arabidopsis thaliana AT1G0.6 Arabidopsis thaliana AT3G040.1 Arabidopsis thaliana AT3G040.2 Arabidopsis thaliana LOC Os03g1.1 Oryza sativa LOC Os03g1.2 Oryza sativa LOC Os07g Oryza sativa LOC Os07g Oryza sativa LOC Os07g Oryza sativa AT4G Arabidopsis thaliana LOC Os04g Oryza sativa LOC Os08g435.1 Oryza sativa LOC Os08g435.3 Oryza sativa LOC Os08g435.2 Oryza sativa AT4G350.1 Arabidopsis thaliana AT1G774.1 Arabidopsis thaliana AT4G083.1 Arabidopsis thaliana AT4G083.2 Arabidopsis thaliana AT4G083.4 Arabidopsis thaliana AT4G083.3 Arabidopsis thaliana LOC Os02g Oryza sativa LOC Os12g020.1 Oryza sativa LOC Os12g030.1 Oryza sativa LOC Os04g350.1 Oryza sativa LOC Os04g350.2 Oryza sativa LOC Os04g350.3 Oryza sativa LOC Os04g350.4 Oryza sativa AT4G Arabidopsis thaliana LOC Os08g410.1 Oryza sativa MGG 077T0 (MoLIP1) Magnaporthe oryzae -15 GLRG 008T0 Colletotrichum graminicola M1.001 e gw Mycosphaerella graminicola GLRG 002T0 Colletotrichum graminicola M1.001 Pa 1 50 Podospora anserina MGG 14940T0 (MoLIP3) Magnaporthe oryzae -15 GLRG 08016T0 Colletotrichum graminicola M1.001 GLRG 09119T0 Colletotrichum graminicola M1.001 GLRG 11144T0 Colletotrichum graminicola M1.001 FGSG 02551T0 Fusarium graminearum FOXG 104T0 Fusarium oxysporum f. sp. lycopersici MGG 10877T0 (MoLIP2) Magnaporthe oryzae -15 GLRG 05589T0 Colletotrichum graminicola M1.001 GLRG 00813T0 Colletotrichum graminicola M1.001 estext Genewise1Plus.C chr Mycosphaerella graminicola e gwh Phanerochaete chrysosporium fgenesh1 pg.c scaffold Phanerochaete chrysosporium e gww Phanerochaete chrysosporium fgenesh1 pm.c scaffold Phanerochaete chrysosporium e gwh Phanerochaete chrysosporium fgenesh1 kg.c scaffold Phanerochaete chrysosporium e gwh Phanerochaete chrysosporium e gww Phanerochaete chrysosporium e gww Phanerochaete chrysosporium fgenesh1 pg.c scaffold 104 Phanerochaete chrysosporium Hetan1.dfl Genemark.2444 g Heterobasidion irregulare TC 32-1 Hetan1.fgenesh2 pm.c scaffold Heterobasidion irregulare TC 32-1 Hetan1.Genemark.2653 g Heterobasidion irregulare TC 32-1 Hetan1.Genemark.7162 g Heterobasidion irregulare TC 32-1 Hetan1.YAI EuGene00355 Heterobasidion irregulare TC 32-1 Hetan1.Genemark.9449 g Heterobasidion irregulare TC 32-1 fgenesh1 pg.c scaffold Phanerochaete chrysosporium estext GeneWisePlus worm.c Laccaria bicolor e gww Phanerochaete chrysosporium fgenesh1 pg.c scaffold Phanerochaete chrysosporium fgenesh1 pg.c scaffold Phanerochaete chrysosporium fgenesh1 pg.c scaffold Phanerochaete chrysosporium fgenesh1 pg.c scaffold 08 Phanerochaete chrysosporium Hetan1.YAI estext fgenesh3 kg.c Heterobasidion irregulare TC 32-1 MGG 004T0 (MoAPX1) Magnaporthe oryzae -15 BC1G 045 Botrytis cinerea MGG 0T0 (MoAPX3) Magnaporthe oryzae -15 FGSG 04434T0 Fusarium graminearum FOXG 138T0 Fusarium oxysporum f. sp. lycopersici MGG 08200T0 (MoAPX2) Magnaporthe oryzae -15 NCU03646T0 Neurospora crassa Pa 2 Podospora anserina GLRG 031T0 Colletotrichum graminicola M1.001 CIMG Coccidioides immitis estext Genewise1.C Heterobasidion irregulare TC 32-1 Hetan1.EuGene Heterobasidion irregulare TC 32-1 Hetan1.estExt fgenesh2 pg.c Heterobasidion irregulare TC 32-1 eu2.lbscf0002g080 Laccaria bicolor BC1G 144 Botrytis cinerea Hetan1.YAI e gw Heterobasidion irregulare TC 32-1 estext fgenesh2 pg.c Laccaria bicolor PITG Phytophthora infestans FGSG 08151T0 Fusarium graminearum FOXG 108T0 Fusarium oxysporum f. sp. lycopersici NCU112T0 Neurospora crassa AT1G Arabidopsis thaliana AT1G Arabidopsis thaliana AT3G Arabidopsis thaliana LOC Os12g262.1 Oryza sativa LOC Os12g262.3 Oryza sativa LOC Os12g262.4 Oryza sativa LOC Os12g262.2 Oryza sativa PITG Phytophthora infestans MGG 000T0 (MoPRX2) Magnaporthe oryzae -15 FGSG 00353T0 Fusarium graminearum FOXG 006T0 Fusarium oxysporum f. sp. lycopersici GLRG 0T0 Colletotrichum graminicola M1.001 ANID Aspergillus nidulans FGSC A4 Afu6g12500 Aspergillus fumigatus Af2 BC1G 139 Botrytis cinerea bgh04156 polypeptide Blumeria graminis f. sp. hordei DH14 HCEG Histoplasma capsulatum H88 BC1G Botrytis cinerea bgh002 polypeptide Blumeria graminis f. sp. hordei DH14 estext fgenesh1 kg.c chr 313 Mycosphaerella graminicola NCU06880T0 Neurospora crassa Pa Podospora anserina orf Candida albicans SC5314 estext Genewise1.C Phycomyces blakesleeanus RO3G 117 Rhizopus oryzae FBpp00 Drosophila melanogaster FBpp0080 Drosophila melanogaster ENSP Homo sapiens ENSP Homo sapiens UM Ustilago maydis 1 estext fgenesh1 kg.c chr 204 Mycosphaerella graminicola SPCC330.06c Schizosaccharomyces pombe e gww Phanerochaete chrysosporium Hetan1.estExt Genewise1Plus.C 288 Heterobasidion irregulare TC 32-1 estext fgenesh1 kg.c 0005 Serpula lacrymans S7.3 e gwh Laccaria bicolor CNAG 032 Cryptococcus neoformans serotype A MGG 02710T0 (MoPRX3) Magnaporthe oryzae -15 GLRG 04713T0 Colletotrichum graminicola M1.001 FGSG 077T0 Fusarium graminearum FOXG 03123T0 Fusarium oxysporum f. sp. lycopersici NCU03151T0 Neurospora crassa Pa Podospora anserina BC1G 032 Botrytis cinerea HCEG Histoplasma capsulatum H88 CIMG Coccidioides immitis ANID Aspergillus nidulans FGSC A4 Afu5g01440 Aspergillus fumigatus Af2 ANID 0 Aspergillus nidulans FGSC A4 Afu6g02280 Aspergillus fumigatus Af2 UM Ustilago maydis 1 e gwh Phanerochaete chrysosporium e gw Serpula lacrymans S7.3 eu2.lbscf0008g03000 Laccaria bicolor estext Genewise1Plus.C Batrachochytrium dendrobatidis JAM81 fgenesh1 pg.c chr Mycosphaerella graminicola YLR109W Saccharomyces cerevisiae S288C orf19.22 Candida albicans SC5314 PGTG Puccinia graminis f. sp. tritici EuGene Melampsora larici-populina AG31 e gw Phycomyces blakesleeanus fgeneshpb pg.3 1 Phycomyces blakesleeanus RO3G 017 Rhizopus oryzae AT1G740.1 Arabidopsis thaliana AT1G650.1 Arabidopsis thaliana AT1G650.1 Arabidopsis thaliana AT1G650.2 Arabidopsis thaliana AT1G650.1 Arabidopsis thaliana LOC Os01g420.1 Oryza sativa AT3G0.1 Arabidopsis thaliana LOC Os02g040.1 Oryza sativa LOC Os06g Oryza sativa AT3G050.1 Arabidopsis thaliana LOC Os01g1.1 Oryza sativa orf19.64 Candida albicans SC5314 BC1G Botrytis cinerea NCU010T0 Neurospora crassa Afu7g068 Aspergillus fumigatus Af2 PGTG Puccinia graminis f. sp. tritici PGTG Puccinia graminis f. sp. tritici PGTG Puccinia graminis f. sp. tritici PGTG Puccinia graminis f. sp. tritici PGTG Puccinia graminis f. sp. tritici PGTG 123 Puccinia graminis f. sp. tritici PGTG 121 Puccinia graminis f. sp. tritici estext Genewise1.C Melampsora larici-populina AG31 fgenesh2 pg.18 # 127 Melampsora larici-populina AG31 Hetan1.estExt Genewise1.C Heterobasidion irregulare TC 32-1 eu2.lbscf0034g01340 Laccaria bicolor e gw Mycosphaerella graminicola MGG 01347T0 (MoCMD1) Magnaporthe oryzae -15 GLRG 04269T0 Colletotrichum graminicola M1.001 FGSG 017T0 Fusarium graminearum FOXG 13514T0 Fusarium oxysporum f. sp. lycopersici FGSG 39T0 Fusarium graminearum FOXG 053T0 Fusarium oxysporum f. sp. lycopersici Pa 2 3 Podospora anserina NCU04594T0 Neurospora crassa BC1G 0 Botrytis cinerea ANID Aspergillus nidulans FGSC A4 Afu1g036 Aspergillus fumigatus Af2 HCEG 055 Histoplasma capsulatum H88 CIMG Coccidioides immitis estext fgenesh1 pg.c chr Mycosphaerella graminicola fgenesh1 pm.c chr Mycosphaerella graminicola ANID 032 Aspergillus nidulans FGSC A4 Afu6g115 Aspergillus fumigatus Af2 FOXG 1T0 Fusarium oxysporum f. sp. lycopersici UM059.1 Ustilago maydis 1 estext Genewise1.C Melampsora larici-populina AG31 gww Phanerochaete chrysosporium e gw Heterobasidion irregulare TC 32-1 estext Genewise1.C 04 Heterobasidion irregulare TC 32-1 estext GeneWisePlus worm.c Laccaria bicolor MGG 02210T0 (MoVPX1) Magnaporthe oryzae -15 fgenesh1 pm.c chr 000 Mycosphaerella graminicola ANID 024 Aspergillus nidulans FGSC A4 RO3G 069 Rhizopus oryzae MGG 074T0 (MoHPX2) Magnaporthe oryzae -15 MGG 1T0 (MoHPX1) Magnaporthe oryzae -15 FGSG 03436T0 Fusarium graminearum FOXG 134T0 Fusarium oxysporum f. sp. lycopersici GLRG 112T0 Colletotrichum graminicola M1.001 NCU11046T0 Neurospora crassa Pa 2 68 Podospora anserina GLRG 028T0 Colletotrichum graminicola M1.001 MGG 071T0 (MoHPX3) Magnaporthe oryzae -15 FGSG 02341T0 Fusarium graminearum FOXG 13077T0 Fusarium oxysporum f. sp. lycopersici ANID Aspergillus nidulans FGSC A4 GLRG 114T0 Colletotrichum graminicola M1.001 Pa 0 3 Podospora anserina BC1G 045 Botrytis cinerea ANID 025 Aspergillus nidulans FGSC A4 ANID 083 Aspergillus nidulans FGSC A4 BC1G Botrytis cinerea estext Genewise1Plus.C chr Mycosphaerella graminicola HCEG Histoplasma capsulatum H88 ANID 023 Aspergillus nidulans FGSC A4 Pa 2 73 Podospora anserina PITG 040 Phytophthora infestans PITG 053 Phytophthora infestans PITG 054 Phytophthora infestans PITG Phytophthora infestans PITG 0 Phytophthora infestans PITG 0 Phytophthora infestans PITG 070 Phytophthora infestans FOXG 13328T0 Fusarium oxysporum f. sp. lycopersici FGSG 08911T0 Fusarium graminearum FOXG 03643T0 Fusarium oxysporum f. sp. lycopersici Pa 2 10 Podospora anserina FOXG 129T0 Fusarium oxysporum f. sp. lycopersici FOXG 158T0 Fusarium oxysporum f. sp. lycopersici GLRG 11820T0 Colletotrichum graminicola M1.001 BC1G 113 Botrytis cinerea Afu4g020 Aspergillus fumigatus Af2 PGTG Puccinia graminis f. sp. tritici EuGene Melampsora larici-populina AG31 EuGene Melampsora larici-populina AG31 EuGene.0015 Melampsora larici-populina AG31 estext Genewise1Plus.C chr 6 Mycosphaerella graminicola fgenesh1 pg.c scaffold Phanerochaete chrysosporium Hetan1.estExt fgenesh2 pm.c Heterobasidion irregulare TC 32-1 estext fgenesh1 kg.c 509 Serpula lacrymans S7.3 estext fgenesh2 pg.c 268 Laccaria bicolor eu2.lbscf0007g03640 Laccaria bicolor RO3G Rhizopus oryzae RO3G Rhizopus oryzae PGTG Puccinia graminis f. sp. tritici estext fgenesh2 pg.c 025 Melampsora larici-populina AG31 PGTG Puccinia graminis f. sp. tritici PGTG Puccinia graminis f. sp. tritici PGTG 135 Puccinia graminis f. sp. tritici fgenesh1 pg.c scaffold Phanerochaete chrysosporium Hetan1.estExt fgenesh3 kg.c Heterobasidion irregulare TC 32-1 estext Genewise1Plus.C 2800 Serpula lacrymans S7.3 estext fgenesh2 pg.c 112 Laccaria bicolor Genemark.2127 g Heterobasidion irregulare TC 32-1 Hetan1.EuGene Heterobasidion irregulare TC 32-1 Hetan1.fgenesh2 pm.c scaffold Heterobasidion irregulare TC 32-1 fgenesh1 kg.1 # 22 # 5 10 CFOT CFPN Serpula lacrymans S7.3 estext fgenesh2 pg.c 4800 Laccaria bicolor FGSG 038T0 Fusarium graminearum FOXG 017T0 Fusarium oxysporum f. sp. lycopersici FOXG 118T0 Fusarium oxysporum f. sp. lycopersici GLRG 07732T0 Colletotrichum graminicola M1.001 BC1G Botrytis cinerea HCEG 037 Histoplasma capsulatum H88 Afu5g000 Aspergillus fumigatus Af2 estext fgenesh1 pg.c chr Mycosphaerella graminicola FGSG 11226T0 Fusarium graminearum FOXG 13304T0 Fusarium oxysporum f. sp. lycopersici BC1G Botrytis cinerea BC1G 064 Botrytis cinerea BC1G 125 Botrytis cinerea fgenesh1 pg.c chr Mycosphaerella graminicola e gw Mycosphaerella graminicola e gw Mycosphaerella graminicola fgenesh1 pg.c chr Mycosphaerella graminicola fgenesh1 pg.c chr Mycosphaerella graminicola estext Genewise1.C chr 5 Mycosphaerella graminicola fgenesh1 pg.c chr 1504 Mycosphaerella graminicola gw Mycosphaerella graminicola BC1G 059 Botrytis cinerea estext fgenesh1 pm.c chr 162 Mycosphaerella graminicola gw Mycosphaerella graminicola estext Genewise1Plus.C chr Mycosphaerella graminicola e gw Mycosphaerella graminicola Pa Podospora anserina e gw Mycosphaerella graminicola estext fgenesh1 kg.c Melampsora larici-populina AG31 estext fgenesh1 pm.c Melampsora larici-populina AG31 estext fgenesh1 kg.c Melampsora larici-populina AG31 fgenesh2 pg.11 # 10 Melampsora larici-populina AG31 estext fgenesh1 kg.c Melampsora larici-populina AG31 fgenesh1 kg.c scaffold Melampsora larici-populina AG31 estext fgenesh1 pg.c 2401 Melampsora larici-populina AG31 fgenesh2 pg.24 # 191 Melampsora larici-populina AG31 fgenesh2 pg. # 26 Melampsora larici-populina AG31 EuGene.0043 Melampsora larici-populina AG31 fgenesh2 pg.69 # 55 Melampsora larici-populina AG31 fgenesh2 pg.69 # 50 Melampsora larici-populina AG31 UM Ustilago maydis 1 UM Ustilago maydis 1 estext fgenesh2 pg.c Laccaria bicolor FOXG 003T0 Fusarium oxysporum f. sp. lycopersici MGG 109T0 (MoLDS1) Magnaporthe oryzae -15 GLRG 11417T0 Colletotrichum graminicola M1.001 FGSG 100T0 Fusarium graminearum FOXG 129T0 Fusarium oxysporum f. sp. lycopersici NCU058T0 Neurospora crassa Pa Podospora anserina BC1G Botrytis cinerea ANID Aspergillus nidulans FGSC A4 Afu3g12120 Aspergillus fumigatus Af2 HCEG Histoplasma capsulatum H88 CIMG Coccidioides immitis Afu4g00180 Aspergillus fumigatus Af2 ANID 017 Aspergillus nidulans FGSC A4 Afu4g107 Aspergillus fumigatus Af2 HCEG Histoplasma capsulatum H88 CIMG Coccidioides immitis estext Genewise1Plus.C chr 13 Mycosphaerella graminicola e gw Mycosphaerella graminicola CIMG Coccidioides immitis MGG 13239T0 (MoLSD2) Magnaporthe oryzae -15 GLRG 13T0 Colletotrichum graminicola M1.001 Pa 1 46 Podospora anserina BC1G 140 Botrytis cinerea bgh017 polypeptide Blumeria graminis f. sp. hordei DH14 FGSG 028T0 Fusarium graminearum FOXG 10645T0 Fusarium oxysporum f. sp. lycopersici GLRG 01818T0 Colletotrichum graminicola M1.001 estext Genewise1Plus.C chr 400 Mycosphaerella graminicola HCEG Histoplasma capsulatum H88 FGSG 11146T0 Fusarium graminearum NCU115T0 Neurospora crassa FOXG 130T0 Fusarium oxysporum f. sp. lycopersici FOXG 15873T0 Fusarium oxysporum f. sp. lycopersici GLRG 11821T0 Colletotrichum graminicola M1.001 estext fgenesh1 pg.c Melampsora larici-populina AG31 fgenesh2 pg.20 # 55 Melampsora larici-populina AG31 estext fgenesh2 pg.c 2000 Melampsora larici-populina AG31 estext fgenesh1 pm.c chr 064 Mycosphaerella graminicola bgh01025 polypeptide Blumeria graminis f. sp. hordei DH14 e gww Phanerochaete chrysosporium fgenesh1 pg.c scaffold Phanerochaete chrysosporium Hetan1.estExt fgenesh2 pm.c 119 Heterobasidion irregulare TC 32-1 estext Genewise1.C 482 Serpula lacrymans S7.3 eu2.lbscf0002g03320 Laccaria bicolor fgenesh1 pg.c scaffold Phanerochaete chrysosporium Hetan1.EuGene Heterobasidion irregulare TC 32-1 eu2.lbscf0068g00150 Laccaria bicolor e gw Serpula lacrymans S7.3 FOXG 1T0 Fusarium oxysporum f. sp. lycopersici FOXG 165T0 Fusarium oxysporum f. sp. lycopersici gwh Phanerochaete chrysosporium ANID Aspergillus nidulans FGSC A4 estext Genewise1.C chr Mycosphaerella graminicola MGG 082T0 (NOX3) Magnaporthe oryzae -15 Pa Podospora anserina FGSG 111T0 Fusarium graminearum FOXG 13287T0 Fusarium oxysporum f. sp. lycopersici GLRG 03348T0 Colletotrichum graminicola M1.001 fgenesh1 pm.c chr Mycosphaerella graminicola AMAG 11331T0 Allomyces macrogynus AMAG 151T0 Allomyces macrogynus MGG 06559T0 (NOX2) Magnaporthe oryzae -15 GLRG 027T0 Colletotrichum graminicola M1.001 NCU107T0 Neurospora crassa Pa 5 80 Podospora anserina FGSG 10807T0 Fusarium graminearum bgh02542 polypeptide Blumeria graminis f. sp. hordei DH14 HCEG Histoplasma capsulatum H88 CIMG Coccidioides immitis FOXG 12738T0 Fusarium oxysporum f. sp. lycopersici PGTG Puccinia graminis f. sp. tritici estext Genewise1.C Melampsora larici-populina AG31 estext fgenesh1 kg.c 400 Melampsora larici-populina AG31 fgenesh1 pg.c scaffold Phanerochaete chrysosporium Hetan1.estExt fgenesh3 kg.c 800 Heterobasidion irregulare TC 32-1 estext Genewise1 human.c 068 Laccaria bicolor fgenesh1 kg.5 # 1016 # 58 2 CFOT CFPN Serpula lacrymans S7.3 estext Genewise1Plus.C 2 Batrachochytrium dendrobatidis JAM81 AMAG 04430T0 Allomyces macrogynus AMAG 181T0 Allomyces macrogynus AMAG 15568T0 Allomyces macrogynus AMAG 110T0 Allomyces macrogynus ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens MGG 000T0 (NOX1) Magnaporthe oryzae -15 FGSG 00739T0 Fusarium graminearum FOXG 010T0 Fusarium oxysporum f. sp. lycopersici GLRG 02946T0 Colletotrichum graminicola M1.001 NCU02110T0 Neurospora crassa Pa Podospora anserina BC1G Botrytis cinerea bgh0 mrna polypeptide Blumeria graminis f. sp. hordei DH14 estext Genewise1Plus.C chr Mycosphaerella graminicola ANID Aspergillus nidulans FGSC A4 Afu6g13350 Aspergillus fumigatus Af2 HCEG 085 Histoplasma capsulatum H88 CIMG Coccidioides immitis e gww Phanerochaete chrysosporium Hetan1.estExt Genewise1Plus.C 112 Heterobasidion irregulare TC 32-1 e gww Laccaria bicolor estext fgenesh1 kg.c Serpula lacrymans S7.3 PGTG 013 Puccinia graminis f. sp. tritici EuGene Melampsora larici-populina AG31 EuGene Melampsora larici-populina AG31 EuGene Melampsora larici-populina AG31 AMAG 133T0 Allomyces macrogynus AT1G0.1 Arabidopsis thaliana AT1G0.2 Arabidopsis thaliana LOC Os01g Oryza sativa LOC Os01g Oryza sativa LOC Os12g350.1 Oryza sativa LOC Os12g350.3 Oryza sativa LOC Os12g350.2 Oryza sativa AT4G250.1 Arabidopsis thaliana AT4G250.2 Arabidopsis thaliana AT5G510.1 Arabidopsis thaliana AT5G073.1 Arabidopsis thaliana AT5G073.2 Arabidopsis thaliana AT5G410.1 Arabidopsis thaliana LOC Os11g Oryza sativa LOC Os11g Oryza sativa LOC Os11g Oryza sativa AT1G130.1 Arabidopsis thaliana AT1G130.2 Arabidopsis thaliana LOC Os08g310.1 Oryza sativa LOC Os09g2.1 Oryza sativa AT1G640.1 Arabidopsis thaliana LOC Os01g Oryza sativa LOC Os05g410.1 Oryza sativa LOC Os05g410.2 Oryza sativa LOC Os05g410.3 Oryza sativa AT4G Arabidopsis thaliana AT3G Arabidopsis thaliana AT5G010.1 Arabidopsis thaliana LOC Os01g880.1 Oryza sativa LOC Os01g880.2 Oryza sativa LOC Os05g380.1 Oryza sativa PITG Phytophthora infestans PITG Phytophthora infestans PITG 108 Phytophthora infestans PITG Phytophthora infestans FBpp Drosophila melanogaster ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens FBpp02811 Drosophila melanogaster F53G12.3 Caenorhabditis elegans F56C11.1 Caenorhabditis elegans ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens UM Ustilago maydis 1 e gw Mycosphaerella graminicola MGG 06442T0 (CATB) Magnaporthe oryzae -15 FGSG 033T0 Fusarium graminearum BC1G 126 Botrytis cinerea GLRG 02234T0 Colletotrichum graminicola M1.001 NCU00355T0 Neurospora crassa Pa 7 10 Podospora anserina bgh00082 polypeptide Blumeria graminis f. sp. hordei DH14 ANID 039 Aspergillus nidulans FGSC A4 Afu3g022 Aspergillus fumigatus Af2 HCEG Histoplasma capsulatum H88 MGG T0 (CATA) Magnaporthe oryzae -15 GLRG 0T0 Colletotrichum graminicola M1.001 NCU081T0 Neurospora crassa Pa Podospora anserina FGSG 06554T0 Fusarium graminearum FOXG 02405T0 Fusarium oxysporum f. sp. lycopersici estext fgenesh1 pm.c chr Mycosphaerella graminicola BC1G Botrytis cinerea bgh00309 polypeptide Blumeria graminis f. sp. hordei DH14 ANID 037 Aspergillus nidulans FGSC A4 Afu6g038 Aspergillus fumigatus Af2 HCEG 039 Histoplasma capsulatum H88 CIMG Coccidioides immitis e gwh Phanerochaete chrysosporium e gww Phanerochaete chrysosporium estext fgenesh1 pg.c 301 Serpula lacrymans S7.3 Hetan1.e gw Heterobasidion irregulare TC 32-1 CNAG 005 Cryptococcus neoformans serotype A CNAG 041 Cryptococcus neoformans serotype A e gw Batrachochytrium dendrobatidis JAM81 e gw Phycomyces blakesleeanus RO3G 027 Rhizopus oryzae RO3G 026 Rhizopus oryzae RO3G 165 Rhizopus oryzae e gw Batrachochytrium dendrobatidis JAM81 e gw Batrachochytrium dendrobatidis JAM81 AT1G Arabidopsis thaliana AT1G Arabidopsis thaliana AT1G Arabidopsis thaliana AT1G Arabidopsis thaliana AT1G Arabidopsis thaliana AT4G350.1 Arabidopsis thaliana AT4G350.2 Arabidopsis thaliana LOC Os03g Oryza sativa LOC Os06g Oryza sativa LOC Os06g Oryza sativa LOC Os02g Oryza sativa LOC Os02g Oryza sativa LOC Os02g Oryza sativa FGSG 065T0 Fusarium graminearum FOXG 02357T0 Fusarium oxysporum f. sp. lycopersici Pa 7 10 Podospora anserina ANID 053 Aspergillus nidulans FGSC A4 Afu2g00200 Aspergillus fumigatus Af2 e gwh Phanerochaete chrysosporium FGSG 02881T0 Fusarium graminearum FOXG 03915T0 Fusarium oxysporum f. sp. lycopersici FOXG 16836T0 Fusarium oxysporum f. sp. lycopersici GLRG 00512T0 Colletotrichum graminicola M1.001 NCU05169T0 Neurospora crassa Pa Podospora anserina BC1G Botrytis cinerea CNAG 056 Cryptococcus neoformans serotype A FOXG 194T0 Fusarium oxysporum f. sp. lycopersici BC1G 010 Botrytis cinerea FGSG 056T0 Fusarium graminearum FOXG 0T0 Fusarium oxysporum f. sp. lycopersici BC1G 0 Botrytis cinerea fgenesh1 pm.c chr Mycosphaerella graminicola CNAG Cryptococcus neoformans serotype A PGTG 148 Puccinia graminis f. sp. tritici e gw Melampsora larici-populina AG31 e gwh Phanerochaete chrysosporium Hetan1.estExt fgenesh3 kg.c Heterobasidion irregulare TC 32-1 fgenesh1 pm.15 # 80 Serpula lacrymans S7.3 fgenesh1 kg.c scaffold Laccaria bicolor GLRG 00027T0 Colletotrichum graminicola M1.001 BC1G 018 Botrytis cinerea ANID Aspergillus nidulans FGSC A4 HCEG 077 Histoplasma capsulatum H88 CIMG Coccidioides immitis SPCC7.07c Schizosaccharomyces pombe YDR256C Saccharomyces cerevisiae S288C orf Candida albicans SC5314 estext Genewise1Plus.C 2012 Phycomyces blakesleeanus RO3G 004 Rhizopus oryzae PITG 148 Phytophthora infestans PITG 1 Phytophthora infestans FBpp Drosophila melanogaster FBpp00733 Drosophila melanogaster ENSP Homo sapiens Y54G11A.13 Caenorhabditis elegans Y54G11A.6 Caenorhabditis elegans Y54G11A.5 Caenorhabditis elegans e gw Melampsora larici-populina AG31 e gw Melampsora larici-populina AG31 e gw Melampsora larici-populina AG31 gw Melampsora larici-populina AG31 gw Mycosphaerella graminicola e gwh Phanerochaete chrysosporium Hetan1.estExt Genewise1Plus.C 257 Heterobasidion irregulare TC 32-1 estext fgenesh1 pg.c 024 Serpula lacrymans S7.3 estext fgenesh1 pm.c Serpula lacrymans S7.3 YGR088W Saccharomyces cerevisiae S288C Afu2g18030 Aspergillus fumigatus Af2 PGTG Puccinia graminis f. sp. tritici PGTG 103 Puccinia graminis f. sp. tritici PGTG Puccinia graminis f. sp. tritici EuGene Melampsora larici-populina AG31 MGG 074T0 (HYR1) Magnaporthe oryzae -15 GLRG 03111T0 Colletotrichum graminicola M1.001 FGSG 050T0 Fusarium graminearum FOXG 068T0 Fusarium oxysporum f. sp. lycopersici NCU034T0 Neurospora crassa BC1G Botrytis cinerea estext fgenesh1 kg.c chr 105 Mycosphaerella graminicola ANID 026 Aspergillus nidulans FGSC A4 CIMG Coccidioides immitis Afu3g122 Aspergillus fumigatus Af2 HCEG 091 Histoplasma capsulatum H88 bgh00083 polypeptide Blumeria graminis f. sp. hordei DH14 Pa 2 Podospora anserina PGTG Puccinia graminis f. sp. tritici fgenesh1 pm.c scaffold Melampsora larici-populina AG31 YBR244W Saccharomyces cerevisiae S288C YIR037W Saccharomyces cerevisiae S288C SPBC32F12.03c Schizosaccharomyces pombe orf19. Candida albicans SC5314 orf19. Candida albicans SC5314 AMAG 03451T0 Allomyces macrogynus AMAG 04771T0 Allomyces macrogynus AMAG 12658T0 Allomyces macrogynus AMAG 14623T0 Allomyces macrogynus UM014.1 Ustilago maydis 1 e gwh Phanerochaete chrysosporium estext fgenesh1 kg.c 0021 Serpula lacrymans S7.3 estext GeneWisePlus human.c 1101 Laccaria bicolor CNAG Cryptococcus neoformans serotype A e gw Batrachochytrium dendrobatidis JAM81 estext fgeneshpb pg.c 036 Phycomyces blakesleeanus estext Genewise1.C Phycomyces blakesleeanus RO3G Rhizopus oryzae RO3G 147 Rhizopus oryzae RO3G Rhizopus oryzae YKL026C Saccharomyces cerevisiae S288C orf Candida albicans SC5314 orf19.87 Candida albicans SC5314 CNAG Cryptococcus neoformans serotype A PITG Phytophthora infestans PITG 134 Phytophthora infestans PITG Phytophthora infestans PITG Phytophthora infestans PITG Phytophthora infestans AT1G634.1 Arabidopsis thaliana AT2G315.1 Arabidopsis thaliana LOC Os11g181.1 Oryza sativa AT4G110.1 Arabidopsis thaliana LOC Os02g Oryza sativa LOC Os04g4.1 Oryza sativa LOC Os04g4.2 Oryza sativa LOC Os04g4.3 Oryza sativa AT2G Arabidopsis thaliana AT2G Arabidopsis thaliana AT2G Arabidopsis thaliana AT4G318.1 Arabidopsis thaliana LOC Os06g0.1 Oryza sativa AT2G Arabidopsis thaliana AT3G Arabidopsis thaliana LOC Os03g Oryza sativa F26E4.12 Caenorhabditis elegans R05H10.5 Caenorhabditis elegans R03G5.5a Caenorhabditis elegans ENSP Homo sapiens FBpp000 Drosophila melanogaster FBpp001 Drosophila melanogaster FBpp002 Drosophila melanogaster FBpp000 Drosophila melanogaster ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens PITG 021 Phytophthora infestans ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens ENSP Homo sapiens C11E4.1 Caenorhabditis elegans C11E4.2 Caenorhabditis elegans FOXG 027T0 Fusarium oxysporum f. sp. lycopersici T09A12.2a Caenorhabditis elegans T09A12.2b Caenorhabditis elegans CAD250.1 Encephalitozoon cuniculi UM Ustilago maydis cystein peroxiredoxin Atypical 2-cystein peroxiredoxin Catalase Cytochrome C Other Class II Hybrid ascorbate- cytochrome C Atypical 2-cystaein Carbosymuconolactone decarboxylase (no activity) Vandium Halo Linolease diol synthase NADP(H) oxidase Catalase Glutathone Figure S1. Phylogenetic analysis of genes in 29 fungal, 1 chromista, 3 metazoa, and 2 viridiplantae genomes (List shown in supplementary Table S1). A neighbor-joining tree was constructed based on the amino acid sequences of all of the genes. The numbers at the nodes indicate bootstrap values (%) in 10,000 bootstrap replicates; only nodes supported by 50 bootstraps or more are shown. Gray boxes are the phylogenetic positions of 27 Magnaporthe oryzae genes. Red and blue bold letters denote M. oryzae and five selected fungal species, respectively.

3 a EcoRI I EcoR I Probe 2.1 Kb 6.2 Kb MoPRX1 EcoR I HPH EcoRI I (Kb) MoPRX1 β-tubulin b Sal I 2.9 Kb Sal I MoCCP1 (Kb) c BamHI 2.3 Kb BamHI MoAPX1 (Kb) Sal I Probe HPH 4.5 Kb Sal I BamHI Probe 3.4 Kb HPH BamHI d XhoI XhoI Probe 4.1 Kb MoAPX2 HPH 5.4 Kb XhoI XhoI (Kb) e EcoRI EcoRI 4.4 Kb 5.1 Kb MoHPX1 EcoRI HPH Probe EcoRI (Kb) f PstI 4.1 Kb MoLDS1 PstI (Kb) 4.1 g Cla I 7.6 Kb TPX1 Cla I (Kb) 7.6 PstI Probe PstI HPH 2.8 Cla I Probe Cla I HPH Kb 3.5 Kb Figure S2. Generation of deletion mutants for selected M. oryzae genes. A total of seven knockout vectors were constructed by PCR amplification. Approximately 1.2~1.5-kb upstream and downstream flanking sequences were amplified using UF/UR and DF/DR, respectively (primer list in Supplementary Table S6), and ligated with the 1.4-kb or 2.1-kb HPH cassette. Southern blots analysis of appropriate enzyme-digested genomic DNAs from the wild-type strain and putative gene deletion mutants. The blots were hybridized with each probe shown in Supplementary Fig. S2 (a-g). For the complementation of Moprx1, putative complemented transformants were generated and tested for transcriptional expression of MoPRX1 gene by RT-PCR (a).

4 a Wild-type ΔMoprx1 Moprx1c 24 hpi 48 hpi Percentage of infection (%) Percentage of infection (%) % No penetration % Start infectious growth % Infectious growth in 1 cell % Infectious hypha in 1-3 cells % Infectious hypha >4 b Wild-type ΔMoprx1 Moprx1c 48 hpi 24 hpi c Percentage of penetration (%) no penetration penetration Wild-type ΔMoprx1 Moprx1c d Tween 20 Wild-type ΔMoPrx1 MoPrx1C Figure S3. Penetration percentage and pathogenicity of Moprx1 on rice sheaths and leaves, and onion epidermal cells. (a) Quantitative measurement of rice sheath assay by Moprx1. Frequency was measured by counting three replicates of - appressoria. (b) Appressorial penetration assay on onion epidermal cell by drop inoculation with conidia/ml and observed at 24 and 48 hpi. Images were captured using a 20-ms exposure to transmitted light with a DIC filter. (c) Percentage of appressorial penetration of onion epidermal cells. Data were obtained by counting three replicates of > appressoria. (d) Infiltration experiment on rice leaves. The conidial suspension ( conidia/ml) was used as an inoculum. Photograph was taken at 5 dpi.

5 Fold change (2 -ddct ) : up-regulation : down-regulation : No differential expression 0.0 Figure S4. Expression profiles of 27 M. oryzae genes in ΔMoprx1. Upregulated genes (more than 1.5-fold) are indicated by red bars, and downregulated genes (less than 0.5-fold) are denoted by blue bars. The genes not showing differential expression are marked in gray.

6 a Wild-type ΔMoprx1 Moprx1c Congo Red (200ppm) b ABTS (0.2mM) c ABTS (0.2mM) + CuSO 4 (0.5mM) Figure S5. Extracellular enzyme activity in ΔMoprx1. The wild-type, ΔMoprx1, and Moprx1c strains were inoculated on complete media (CM) containing (a) 200 ppm Congo Red, and (b) 0.2 mm ABTS, and (c) 0.2 mm ABTS with 0.5 mm CuSO 4. The 5-mm-diameter mycelial blocks were inoculated onto media, and incubated at 25 C. ABTS discoloration was observed at 4 dpi and Congo Red discoloration at 9 dpi.

7 Table S1. Peroxidase genes from 29 fungal, thee metazoan, two viridiplantae and one chromistal genomes Kingdom Phylum/ No. of Haem Non-haem Subphylum Species name Peroxidses Animal per oxidase-cy Catalase su clooxygena perfamily se superfa mily Class I ClassII Alkylhydr DyP-type NAD(P)H operoxida peroxidas oxidase su se D-like e perfamily superfami ly Glutathio ne peroxi dase Halopero xidase Peroxired oxin Chromista Oomycota Phytophthora infestans Aspergillus fumigatus Af Aspergillus nidulans Blumeria graminis f.sp. hordei Botrytis cinerea Candida albicans SC Coccidioides immitisrs Colletotrichum graminicola M Ascomycota Fusarium graminearum Fusarium oxysporum Histoplasma capsulatum H Magnaporthe oryzae Mycosphaerella graminicola Neurospora crassa Podospora anserina Fungi Saccharomyces cerevisiae S288C Schizosaccharomyces pombe Cryptococcus neoformans var. grubii H Heterobasidion irregulare TC Laccaria bicolor Basidiomycota Melampsora larici-populina AG Phanerochaete chrysosporium Puccinia graminis f. sp. tritici Serpula lacrymans S Ustilago maydis Blastocladiomycota Allomyces macrogynus Chytridiomycota Batrachochytrium dendrobatidis JAM Microsporidia Encephalitozoon cuniculi Zygomycota Phycomyces blakesleeanus Rhizopus oryzae Arthropoda Dorosophila melanogaster Metazoa Chordata Homo sapiens Nematoda Caenorhabditis elegans Viridiplantae Streptophyta Arabidopsis thaliana Oryza sativa (japonica cultivar-group) Total

8 Table S2. Predicted genes in M. oryzae Haem Non haem Class Type Locus Class I Class II NAD(P)H oxidase superfamily DyP-type Catalase superfamily Animal Hybrid Ascorbate- Cytochrome C Catalase Cytochrome C Other class II NADP(H) oxidase Halo (haem) Catalase Linoleate diol synthase (PGHS like) Protein Signal Length peptide Annotation MGG_004 4 hypothetical protein, MoAPX1 MGG_ V hypothetical protein, MoAPX2 MGG_0 548 V hypothetical protein, MoAPX3 MGG_ catalase- 1, CPXA MGG_034 6 V catalase- 2, CPXB MGG_ cytochrome c, MoCCP1 MGG_ cytochrome c, MoCCP2 MGG_ V ligninase H2, MoLIP1 MGG_ V hypothetical protein, MoLIP2 MGG_ ligninase C, MoLIP3 MGG_ cytochrome b-245 heavychain subunit beta, NOX1 MGG_ cytochrome b-245 heavychain subunit beta, NOX2 MGG_082 9 hypothetical protein, NOX3 MGG_1 242 V hypothetical protein, MoHPX1 MGG_ V hypothetical protein, MoHPX2 MGG_ V hypothetical protein, MoHPX3 MGG_ 743 catalase-1, CATA MGG_ V catalase-3, CATB MGG_ heme, MoLDS1 MGG_ linoleate diol synthase, MoLDS2 1-Cysteine peroxiredoxin MGG_ mitochondrial peroxiredoxin PRX1, MoPRX1 Peroxiredoxin Atypical 2-Cysteine peroxiredoxin (type Q, BCP) MGG_ thioredoxin, TPX1 Atypical 2-Cysteine MGG_000 1 AphC/TSA family protein, MoPRX2 peroxiredoxin (type II &V) MGG_ peroxiredoxin type-2, MoPRX3 Halo Vanadium chloro MGG_ vanadium chloro, MoVPX1 Alkyhydroperox Carboxymuconolactone idase D-like decarboxylase (no MGG_ superfamily activity) Glutathione Fungi-Bacteria glutathione 4-carboxymuconolactone decarboxylase, MoCMD1 MGG_074 1 peroxiredoxin HYR1, HYR1

9 Table S3: List of 27 putative proteins in Magnaporthe oryzae and Blast Matrix results compared to those from 35 organisms Kingdom Chr omi sta Fungi Metazoa Viridipla ntae sub-family Protein name Phylum Oo myc ota Ascomycota Basidiomycota Blast Chyt Micr oclad ridio ospo iomy myc ridia cota ota Zygomyc ota Arthr opod a Chor data Nem atod a Streptoph uta Species Pi * Af1 An Bg Bc Cas Ci Cg Fg Fol Hc8 Mo Mgr Nc Pa Sc Sp Cn Ha Lb Mlp Pc Pg Sl7 Um5 Am BdJ Ecu Pbl Ro Dm Hs Ce Ath Osj Hybrid Ascorbate- Cytochrome C Catalase Cytochrome C Other class II NADP(H) oxidase Halo (haem) Catalase Linoleate diol synthase (PGHS like) Peroxiredoxin Vanadium chloro Carboxymuconolactone decarboxylase Fungi-Bacteria glutathione MoAPX MoAPX MoAPX CPXA CPXB MoCCP MoCCP MoLIP MoLIP MoLIP NOX NOX NOX MoHPX MoHPX MoHPX CATA CATB MoLDS MoLDS MoPRX TPX MoPRX MoPRX MoVPX MoCMD HYR : 1e- ; ± : 1e-50 ; + : 1e-30 ; + : 1e-10 ; - : None *Abbreviation for fungal species and other organisms: Pi: Phytophthora infestans; Af1: Aspergillus fumigatus Af2; An: Aspergillus nidulans; Bg: Blumeria graminis f.sp. hordei; Bc: Botrytis cinerea; Cas: Candida albicans SC5314; Ci: Coccidioides immitis; Cg: Colletotrichum graminicola M1.001; Fg: Fusarium graminearum; Fol: Fusarium oxysporum; Hc8: Histoplasma capsulatum H88; Mo: Magnaporthe oryzae -15; Mgr: Mycosphaerella graminicola; Nc: Neurospora crassa; Pa: Podospora anserina; Sc: Saccharomyces cerevisiae S288C; Sp: Schizosaccharomyces pombe; Cn: Cryptococcus neoformans variety gatti (serotype A) ; Ha: Heterobasidion annosum; Lb: Laccaria bicolor; Mlp: Melampsora larici-populina AG31; Pc: Phanerochaete chrysosporium; Pg: Puccinia graminis; Sl7: Serpula lacrymans S7.3; Um5: Ustilago maydis 1; Am: Allomyces macrogynus; Bdj: Batrachochytrium dendrobatidis JAM81; Ec: Encephalitozoon cuniculi; Pbl: Phycomyces blakesleeanus; Ro: Rhizopus oryzae; Dm: Dorosophila melanogaster; Hs: Homo sapiens; Ce: Caenorhabditis elegans; Ath: Arabidopsis thaliana; Osj: Oryza sativa japonica.

10 Table S4. Characterization of transformants including deletion mutants for seven genes in M. oryzae pathogenicity-related phenotypes Strain Conidiation (10 5 / ml) a Conidial germination (%) b Appressorium formation (%) c Wild-type 55.7±1.5 E d.7±1.5 B.3±1.5 BC ΔMoprx1 42.7±7.5 B.7±1.2 AB.0±3.6 A Moprx1c 56.3±1.5 E 94.3±1.5 A.0±1.0 ABC ΔMoccp1 49.0±1.0 CD.3±0.6 AB.0±2.6 ABC ΔMoapx1 57.3±1.2 E.7±0.6 AB 94.7±2.1 AB ΔMoapx2 44.7±2.3 BC 94.7±1.5 A.7±0.6 BC ΔMohpx1 50.3±1.5 D.7±0.6 B.3±0.6 C ΔMolds1 47.0±1.0 BCD.7±1.5 AB.0±1.0 ABC Δtpx1 10.7±2.1 A.7±1.5 AB.0±1.0 ABC a Conidiation was measured by counting the number of conidia collected with 5 ml of sterilized distilled water from 7-day-old V8 juice agar plate; b Percentage of conidial germination on hydrophobic surfaces was measured under a light microscope with three replicates; c Percentage of appressorium formation on hydrophobic surfaces was measured using germinated conidia with three replicates; d Duncan s test was used to determine significance at the % probability level. The same letters in a column showed no significant difference.

11 Table S5. Total RNA was extracted from five conditions including infection-related developments (conidia, germinated conidia, appressoria, and infectious growth) and oxidative stress treatment Categories Conditions Conc. Infection-related developments Conidia Germinated conidia Appressoria hpi on rice Treated time Basal medium for treatment Oxidative stress H 2 O mm 4 h CM Note: Germinated conidia and appressoria were harvested after 4 h and 16 h, respectively, on hydrophobic surface for RNA extraction.

12 Table S6. List of primers used in this study Primer name Sequence (5 3 ) Knock-out constructs MoPRX1 MoPRX1_UF MoPRX1_UR MoPRX1_DF MoPRX1_DR MoPRX1_UNF MoPRX1_DNR MoPRX1_UF_F MoPRX1_DF_R MoCCP1 MoCCP1_UF MoCCP1_UR MoCCP1_DF MoCCP1_DR MoCCP1_UNF MoCCP1_DNR MoCCP1_UR_F MoCCP1_DF_R MoAPX1 MoAPX1_UF MoAPX1_UR MoAPX1_DF MoAPX1_DR MoAPX1_UNF MoAPX1_DNR MoAPX1_UR_F MoAPX1_DF_R MoAPX2 MoAPX2_UF MoAPX2_UR MoAPX2_DF MoAPX2_DR MoAPX2_UNF MoAPX2_DNR MoAPX2_UR_F MoAPX2_DF_R MoHPX1 MoHPX1_UF MoHPX1_UR MoHPX1_DF MoHPX1_DR MoHPX1_UNF MoHPX1_DNR MoHPX1_UR_F MoHPX1_DF_R MoLDS1 MoLDS1_UF MoLDS1_UR MoLDS1_DF TTC CAG GGG TAC TAT GGC AAC AA CCT CCA CTA GCT CCA GCC AAG CC AAT CCG GGC CCA GTT TAG GTT TAT GTT GGT GTC GAT GTC AGC TCC GGA G TCA ATA TGG CGC CGC AGC AGT AAT GTACTCGCCTGCCCTGCCTATCTT GCTGCTAAAGGGATTGGCTACTCA ATGGTCGCCGCTATTTGCTTATG ATAAACCTAAACTGGGCCCGGATT ATTACTGCTGCGGCGCCATATTGA TCT CGT TCC TGC AGA TGC CGT C CCT CCA CTA GCT CCA GCC AAG CC TGA GGT CTC TGA CTT CAG TGG TAG GT GTT GGT GTC GAT GTC AGC TCC GGA G TTG GGG AGG ACT TGA TGG TTA GAC TTG CAG CCT GGA CTC GAG CAA CAC GCC GGA ACT TCC AGT TTG AAG ACA ACT ACC TA AAA CTG AGC GAG GAC TAC GAC GCC ACC TAC CAC TGA AGT CAG AGA CCT CA CAA GTC TAA CCA TCA AGT CCT CCC CAA CAG TGG AGG AAT CAG CAA CTC TCA GC CCT CCA CTA GCT CCA GCC AAG CC CCA TTG CCT TTA TGC GGA AGT TCT TGT GTT GGT GTC GAT GTC AGC TCC GGA G CAT GAG CGC GTT TCA CAG GAC TC TGA CAC GCC AAG ACC TAC CAC AGA G AAG GGG AAA CAT TGC GCA GAC ATC ACA CTT GCC AGC CTA TCT TGT CCA GTA GTG G ACA AGA ACT TCC GCA TAA AGG CAA TGG GAG TCC TGT GAA ACG CGC TCA TG GCG GCT ATA GAA AGA TCC GGC ATG AAT CCT CCA CTA GCT CCA GCC AAG CC ACA GCA GAT TGT GTG GTG ATG CAC GTT GGT GTC GAT GTC AGC TCC GGA G GGA TGA GAA TGA GCC AAG CAG GGA ATT TCG GAG ACG CCT AGC CTT CCC TA AAG GTC TCT TAA GGC ACA TTT CGT GCG G TGC CCC TAG AAC TCG TTA GTC AGT CTA GTT CG GTG CAT CAC CAC ACA ATC TGC TGT AAT TCC CTG CTT GGC TCA TTC TCA TCC TCGTGAGAGGTGAGGACAATAAAC CCT CCA CTA GCT CCA GCC AAG CC CTG CCA AAG GAC GAA GGA TTG AAA GTT GGT GTC GAT GTC AGC TCC GGA G ACT GCT GTT TGA GAT GTC CA CAA CAC GCA TGA CTC CAC CTA CAA TGG CTA GAC ATC TGA GGT TAT CTG GTT CGA GGA TGT TTA GTT TTT CAA TCC TTC GTC CTT TGG CAG TGG ACA TCT CAA ACA GCA GT GGT AAG TAA CAT GCT AGG CAT TTG GAG TCT CG CCT CCA CTA GCT CCA GCC AAG CC GCA ATG GGA ACG CCA TCG TGT ATT GTT GGT GTC GAT GTC AGC TCC GGA G TGA CTG TTG GTG GGA TAA GAC TGA ATG AA