CELL REPLICATION. Fluorescent light microscopy showing mitosis, especially immunolabelled cytoskeleton and tubulin
|
|
|
- Eileen Welch
- 5 years ago
- Views:
Transcription
1 CELL REPLICATION Fluorescent light microscopy showing mitosis, especially immunolabelled cytoskeleton and tubulin
2 Cell REPLICATION PROLIFERATION MUTIPLICATION DIVISION
3
4
5 CELL REPLICATION Fluorescent light microscopy showing mitosis, especially immunolabelled actin cytoskeleton and tubulin
6 EM showing mitosis in a satellite cell
7 main METHODS 1. Incorporation of EXOGENOUS label into newly synthesised DNA Radioactive tritiated thymidine and detect by autoradiography Thymidine analogue, 5 bromode-oxyuridine (BrdU) and detect by antibody 2. Detection of ENDOGENOUS protein expressed only in replication cells
8 main METHODS 1. Incorporation of EXOGENOUS label into newly synthesised DNA Radioactive tritiated thymidine and detect by autoradiography Thymidine analogue, 5 bromode-oxyuridine (BrdU) and detection by antibody 2. Detection of ENDOGENOUS protein expressed only in replication cells
9 Picture of gut
10
11 (1a) Tritiated thymidine Exogenous radioactive label incorporated during DNA synthesis Detection by autoradiography shows LOCATION of labelled nuclei/cells Cut fixed tissue sections onto slides. Dip slides into photographic emulsion. Leave for weeks in the dark. Decay of isotope affects emulsion virtual image. Develop film: causes silver grains to deposit. Observe silver grains in emulsion (above tissue)
12 Sampled at 1 hour after labelling = pre-mitotic nuclei labelled
13 Sampled at 2 weeks after labelling = post-mitotic muscle nuclei labelled
14 Tissues sampled at 2 weeks after label = labelled post-mitotic muscle nuclei CRUSH INJURY
15 Compare CRUSH INJURY with events after MUSCLE GRAFTING
16
17 (1a) Tritiated thymidine Exogenous radioactive label incorporated during DNA synthesis Advantages 1. persistent label in new DNA 2. can track fate of labelled cells where it moves. 3. labels daughter cells and can track 4. can calculate number of divisions. Disadvantages 1. radioisotopes 2. need to administer the label to cells or animals 3. dilution of the label/nucleus with each cell division 4. possible re-utilisation of label (if cell dies and is phagocytosed by macrophages)
18 (1a) Tritiated thymidine Exogenous radioactive label incorporated during DNA synthesis QUANTITATION by scintillation counting Measures TOTAL label present
19
20 TISSUE CULTURE: quantitation of cell proliferation 96 well plate Allows comparison of many culture conditions. Samples often in triplicate. METHOD (to QUANTITATE cell proliferation) Cells in culture (96 well plate) grown +/- growth factors for various times. Before sampling, wells are exposed to tritiated thymidine for 4 hrs. Radioactivity is incorporated into new DNA (nuclei in S phase) of cells Excess tritiated thymidine is washed off. Radioactivity of ALL cells/well is measured in a scintillation counter Total radioactivity measures the number of cells/myoblasts in S phase. Compare mitogenic effects of different growth factors
21 Effect of Growth Factors on Myoblast Proliferation Fold Increase in 3 H-thymidine Uptake Compare cells from 2 strains of mice bfgf Growth factor (ng/ml) PDGF METHOD (to TGF-β QUANTITATE 1 cell proliferation) SJL/J In response to different concentrations of GF BALB/c in vitro Compare 2 GF (FGF and PDGF) SHOWS 0 0 that.0 1 FGF 0.1 has 1 greater 1 0 mitogenic effect. PDGF has little effect at low conc. and has less effect on proliferation
22 (1b) Bromodeoxyuridine (BrdU) BrdU is an analogue of thymidine. Exogenous BrdU is incorporated during DNA synthesis BrdU is detected by an ANTIBODY
23 Primary muscle cell culture BrdU = red (DNA synthesis in nucleus) Desmin = green (identifies myoblasts)
24 (1b) BrdU Exogenous label incorporated during Advantages DNA synthesis 1-4 as for tritiated thymidine 5. Antibody detection is very quick and not hazardous. Disadvantages 2-4 as for tritiated thymidine. BrdU can be toxic and interfere with cell biology. Therefore usually used for short term studies i.e label and sample, especially in tissue culture.
25 (2) ENDOGENOUS proteins that change during the cell cycle e.g proliferating cell nuclear antigen (PCNA) No need to add anything to label the cells: Just DETECT what is already there Use an antibody to detect
26 PCNA immunostaining (brown) identifies replicating cells in damaged skeletal muscle tissue (TS). Double staining needed to identify cell type: many of these may be macrophages..
27 Tissue Culture. Immunostaining to detect proteins involved in cell proliferation: - PCNA (or Ki67) COUNT labelled nuclei to measure numbers of proliferating cells (Can double stain to identify specific cell e.g myoblasts) Tissue Culture Immunostaining to detect proteins involved in cell proliferation -PCNA -Ki67
28 (2) ENDOGENOUS proteins that change during the cell cycle e.g proliferating cell nuclear antigen (PCNA) Advantage 1. No need to add anything to label the cells 2. Detect by antibody Disadvantage 1. Detects cells while they are replication. Therefore no good for tracking cell FATE.
29 (3) Cell assays to measure NUMBER of cells by metabolites produced e.g CellTiter assay No need to add anything to label the cells: Just DETECT what is already there
30 Cell Metabolites The CellTiter 96 assay is a colorimetric method that determines the number of viable cells in a culture. A component of the assay system, Owen s reagent, is reduced by living cells to a soluble coloured formazan product. Colour development is monitored by recording the absorbance at 490nm. The extent of colour development directly relates to the quantity of formazan product which is in turn directly proportional to the number of living cells. Measures NUMBER. This is balance of cell proliferation AND cell death: need to consider.
31 Cell Replication You have labelled a population of replicating cells in the developing brain of a newborn mouse by systemic injection of tritiated thymidine. You sample several brains from the young animals within one day of injection to determine the initial level of labelling. One year later you sample the other brains. With respect to the initial population of labelled cells you want to know: if they divided many times after birth where they moved to in the brain if any of them were lost from the tissue (died or exited). CLASS EXERCISE With respect to the above 3 points: 1. What would examination of tissue sections tell you? 2. What would extraction of the total DNA and scintillation counting tell you? 3. If the labelled cells had divided many times during the year (but none had been lost) what would you see on tissue sections and what would you see on scintillation counting? THREE GROUPS WILL EACH FOCUS ON ONE OF THESE QUESTIONS. 15 MINUTES TO DISCUSS AND THEN GIVE A QUICK VERBAL REPORT
32
33
34
SUPPLEMENTARY INFORMATION
doi:10.1038/nature09414 Supplementary Figure 1 FACS-isolated 8c hepatocytes are highly pure. a, Gating strategy for identifying hepatocyte populations based on DNA content. b, Detection of mchry and mchr9
5.1 Cell Division and the Cell Cycle
5.1 Cell Division and the Cell Cycle Lesson Objectives Contrast cell division in prokaryotes and eukaryotes. Identify the phases of the eukaryotic cell cycle. Explain how the cell cycle is controlled.
E. Incorrect! At telophase II, cells are nearly completed with meiosis, with no cross-over.
OAT Biology - Problem Drill 06: Mitosis and Meiosis Question No. 1 of 10 1. During meiosis, cross-over between homologous chromosomes occurs at the end of. Question #01 (A) Anaphase II (B) Metaphase I
Topic 3: Genetics (Student) Essential Idea: Chromosomes carry genes in a linear sequence that is shared by members of a species.
Topic 3: Genetics (Student) 3.2 Essential Idea: Chromosomes carry genes in a linear sequence that is shared by members of a species. 3.2 Chromosomes 3.2.U1 Prokaryotes have one chromosome consisting of
DONALD E. WIMBER, Ph.D., HENRY QUASTLER, M.D., OTTO L. STEIN, Ph.D., and DORIS R. WIMBER
Published Online: 1 October, 1960 Supp Info: http://doi.org/10.1083/jcb.8.2.327 Downloaded from jcb.rupress.org on August 15, 2018 ANALYSIS OF TRITIUM INCORPORATION INTO INDIVIDUAL CELLS BY AUTO- RADIOGRAPHY
Assignment 7 Due February 26
Assignment 7 Due February 26 Cells of Multicellular organisms 1. File upload (3 points) View this electron micrograph of spinach leaf cells. The central cell has a thin cell wall; it is difficult to distinguish
Key Concepts. n Cell Cycle. n Interphase. n Mitosis. n Cytokinesis
The Cell Cycle B-2.6: Summarize the characteristics of the cell cycle: interphase (G 1, S, G 2 ); the phases of mitosis (prophase, metaphase, anaphase, telophase); and plant and animal cytokinesis. Key
SUPPLEMENTARY INFORMATION
doi:10.1038/nature11419 Supplementary Figure 1 Schematic representation of innate immune signaling pathways induced by intracellular Salmonella in cultured macrophages. a, During the infection Salmonella
Identifying cells using Immunofluorscence Rachel Scalzo Bio-300, 001 Professor Bentley 9/24/2014
Identifying cells using Immunofluorscence Rachel Scalzo Bio-300, 001 Professor Bentley 9/24/2014 2 Abstract In this experiment, we used immunofluorescence to isolate certain organelles and see where they
ADME studies with radiolabeled compounds. Biomedical Applications of Radioisotopes and Pharmacokinetics Unit
ADME studies with radiolabeled compounds Miguel Ángel Morcillo Alonso Biomedical Applications of Radioisotopes and Pharmacokinetics Unit CIEMAT (Centro de Investigaciones Energéticas, Medioambientales
Class XI Chapter 10 Cell Cycle and Cell Division Biology
Question 1: What is the average cell cycle span for a mammalian cell? The average cell cycle span for a mammalian cell is approximately 24 hours. Question 2: Distinguish cytokinesis from karyokinesis.
NCERT. not to be published CHAPTER 10 CELL CYCLE AND CELL DIVISION 10.1 CELL CYCLE 162 BIOLOGY
162 BIOLOGY 10.1 Cell Cycle 10.2 M Phase 10.3 Significance of Mitosis 10.4 Meiosis 10.5 Significance of Meiosis CHAPTER 10 CELL CYCLE AND CELL DIVISION Are you aware that all organisms, even the largest,
The synthesis and migration of nuclear proteins during mitosis and differentiation of cells in rats. Summary
229 The synthesis and migration of nuclear proteins during mitosis and differentiation of cells in rats By R. T. SIMS (From the Department of Anatomy, McGill University, Canada, and Department of Anatomy,
10.1 Growth and Cell Reproduction
10.1 Growth and Cell Reproduction Growth is a characteristic of all living things. You started out as a single cell. That cell quickly divided into two cells. Two cells became four and four became eight.
Cell Division and Reproduction
Cell Division and Reproduction What do you think this picture shows? If you guessed that it s a picture of two cells, you are right. In fact, the picture shows human cancer cells, and they are nearing
Chapter 5: Mitosis is the Basis of Asexual Reproduction
Chapter 5: Mitosis is the Basis of Asexual Reproduction Section 5.1: The Cell Cycle and Mitosis Living things must be able to reproduce. For unicellular organisms, cell reproduction is necessary to maintain
Class XI Chapter 10 Cell Cycle and Cell Division Biology
Question 1: What is the average cell cycle span for a mammalian cell? The average cell cycle span for a mammalian cell is approximately 24 hours. Question 2: Distinguish cytokinesis from karyokinesis.
Mitosis, development, regeneration and cell differentiation
Mitosis, development, regeneration and cell differentiation Mitosis is a type of cell division by binary fission (splitting in two) which occurs in certain eukaryotic cells. Mitosis generates new body
cycle & cell the division
the cycle & celldivision the cell cycle Most cells in an organism go through a cycle of growth, development, and division called the cell cycle. The cell cycle makes it possible for organisms to grow and
Monitoring neurite morphology and synapse formation in primary neurons for neurotoxicity assessments and drug screening
APPLICATION NOTE ArrayScan High Content Platform Monitoring neurite morphology and synapse formation in primary neurons for neurotoxicity assessments and drug screening Suk J. Hong and Richik N. Ghosh
Chapter 6: Cell Growth and Reproduction Lesson 6.1: The Cell Cycle and Mitosis
Chapter 6: Cell Growth and Reproduction Lesson 6.1: The Cell Cycle and Mitosis No matter the type of cell, all cells come from preexisting cells through the process of cell division. The cell may be the
A. Incorrect! The Cell Cycle contains 4 distinct phases: (1) G 1, (2) S Phase, (3) G 2 and (4) M Phase.
Molecular Cell Biology - Problem Drill 21: Cell Cycle and Cell Death Question No. 1 of 10 1. Which of the following statements about the cell cycle is correct? Question #1 (A) The Cell Cycle contains 3
Question 1: What is the average cell cycle span for a mammalian cell? The average cell cycle span for a mammalian cell is approximately 24 hours. Question 2: Distinguish cytokinesis from karyokinesis.
Foundation Cell Biology
Foundation Cell Biology Electron vs Light Microscope Light use light and lenses to magnify specimen Electron use a beam of electrons to form an image Electron higher magnification and higher resolution
7.1 Atomic Theory and Radioactive Decay
7.1 Atomic Theory and Radioactive Decay exists all around us. This radiation consists of high energy particles or waves being emitted from a variety of materials. is the release of high energy particles
CHAPTER 10 CELL CYCLE AND CELL DIVISION MULTIPLE CHOICE QUESTIONS
CELL CYCLE AND CELL DIVISION 49 49 CHAPTER 10 CELL CYCLE AND CELL DIVISION MULTIPLE CHOICE QUESTIONS 1. Meiosis results in a. Production of gametes b. Reduction in the number of chromosomes c. Introduction
Chapter 9 Active Reading Guide The Cell Cycle
Name: AP Biology Mr. Croft Chapter 9 Active Reading Guide The Cell Cycle 1. Give an example of the three key roles of cell division. Key Role Reproduction Example Growth and Development Tissue Renewal
Revision checklist. Step Learning outcome Had a look Nearly there Nailed it!
Radioactivity a Atomic models Describe the structure of an atom (in terms of nucleus and electrons). State where most of the mass of an atom is found. State the sizes of atoms and small molecules. Describe
3.a.2- Cell Cycle and Meiosis
Big Idea 3: Living systems store, retrieve, transmit and respond to information essential to life processes. 3.a.2- Cell Cycle and Meiosis EU 3.A: Heritable information provides for continuity of life.
Atomic Notation (or Nuclear Symbol): Shorthand for keeping track of protons and neutrons in the nucleus
Name Section CHM52LL: Nuclear Chemistry: Radioactivity, Decay, Dating, and Other Hazards There is no prelab assignment this week I. Radioactive Isotopes and Nuclear Equations Atoms are composed of three
10.1 RADIOACTIVE DECAY
10.1 RADIOACTIVE DECAY When Henri Becquerel placed uranium salts on a photographic plate and then developed the plate, he found a foggy image. The image was caused by rays that had not been observed before.
Animal Cell Organelles. Plant Cell. Organelle. Cell Wall. Chloroplasts. Vacuole
Cell Biology Higher Electron vs Light Microscope Light use light and lenses to magnify specimen Electron use a beam of electrons to form an image Electron higher magnification and higher resolution Electron
The Cell Cycle and Cell Division. What are the phases of the cell cycle? Why is the result of the cell cycle important?
The Cell Cycle and Cell Division What are the phases of the cell cycle? Why is the result of the cell cycle important? The Cell Cycle and Cell Division cell cycle interphase sister chromatid centromere
Biology: Life on Earth
Biology: Life on Earth Eighth Edition Lecture for Chapter 11 The Continuity of Life: Cellular Reproduction Cellular Reproduction Intracellular activity between one cell division to the next is the cell
Lecture Presentation. Chapter 21. Nuclear Chemistry. James F. Kirby Quinnipiac University Hamden, CT Pearson Education, Inc.
Lecture Presentation Chapter 21, Inc. James F. Kirby Quinnipiac University Hamden, CT Energy: Chemical vs. Chemical energy is associated with making and breaking chemical bonds. energy is enormous in comparison.
Dividing by 2 10/15/2015. Unit 3: Atomic Structure - The Nucleus Lesson 3.5: Half-Life 99
/15/2015 Dividing by 2 Unit 3: Atomic Structure - The Nucleus Lesson 3.5: Half-Life 99 The Random Nature of the Universe Quantum mechanics says that it is impossible to know when any particular atom is
2. is the period of growth and development for a cell. 3. During interphase, most cells go through three stages rapid growth and
Chapter 5 Lesson 1- General Lesson Outline Directions: Use the words below to fill in the outline of the text from lesson one. If the word is used more than once, it is followed by the number of times
Supplementary Figure 1. Markedly decreased numbers of marginal zone B cells in DOCK8 mutant mice Supplementary Figure 2.
Supplementary Figure 1. Markedly decreased numbers of marginal zone B cells in DOCK8 mutant mice. Percentage of marginal zone B cells in the spleen of wild-type mice (+/+), mice homozygous for cpm or pri
Supporting Information
Supporting Information Sulfonate groups and saccharides as essential structural elements in heparin-mimicking polymers used as surface modifiers: optimization of relative contents for anti-thrombogenic
P7 Radioactivity. Student Book answers. P7.1 Atoms and radiation. Question Answer Marks Guidance
P7. Atoms and radiation a radiation from U consists = particles, radiation from lamp = electromagnetic waves, radiation from U is ionising, radiation from lamp is non-ionising b radioactive atoms have
Introduction: The Cell Cycle and Mitosis
Contents 1 Introduction: The Cell Cycle and Mitosis 2 Mitosis Review Introduction: The Cell Cycle and Mitosis The cell cycle refers to the a series of events that describe the metabolic processes of growth
Formation of the Cortex
Formation of the Cortex Neuronal Birthdating with 3 H-thymidine 3H-thymidine is incorporated into the DNA during the S-phase (replication of DNA). It marks all mitotic cells Quantitative technique. (you
General Physics (PHY 2140)
General Physics (PHY 2140) Lecture 19 Modern Physics Nuclear Physics Nuclear Reactions Medical Applications Radiation Detectors Chapter 29 http://www.physics.wayne.edu/~alan/2140website/main.htm 1 Lightning
General Physics (PHY 2140)
General Physics (PHY 2140) Lightning Review Lecture 19 Modern Physics Nuclear Physics Nuclear Reactions Medical Applications Radiation Detectors Chapter 29 http://www.physics.wayne.edu/~alan/2140website/main.htm
Mitochondrial Dynamics Is a Distinguishing Feature of Skeletal Muscle Fiber Types and Regulates Organellar Compartmentalization
Cell Metabolism Supplemental Information Mitochondrial Dynamics Is a Distinguishing Feature of Skeletal Muscle Fiber Types and Regulates Organellar Compartmentalization Prashant Mishra, Grigor Varuzhanyan,
Year 09 Science Learning Cycle 5 Overview
e Year 09 Science Learning Cycle 5 Overview Learning Cycle Overview: Biology How do we keep your body healthy L01 4.3.1.1 Communicable (infectious) disease L02 4.3.1.2 Viral diseases L03 4.3.1.3 Bacterial
Cell Cycle and Mitosis
Cell Cycle and Mitosis THE CELL CYCLE The cell cycle, or cell-division cycle, is the series of events that take place in a eukaryotic cell between its formation and the moment it replicates itself. These
The Microscopic Observation of Mitosis in Plant and Animal Cells
The Microscopic Observation of Mitosis in Plant and Animal Cells Prelab Assignment Before coming to lab, read carefully the introduction and the procedures for each part of the experiment, and then answer
THE SYNTHESIS OF DNA THROUGH THE CELL CYCLE OF AMOEBA PROTEUS
J. Cell Sci. 3, 483-491 (1968) 483 Printed in Great Britain THE SYNTHESIS OF DNA THROUGH THE CELL CYCLE OF AMOEBA PROTEUS M. J. ORD* Department of Zoology, The University, Southampton, England SUMMARY
Radioactivity. Ernest Rutherford, A New Zealand physicist proved in the early 1900s a new model of the atom.
Radioactivity In 1896 Henri Becquerel on developing some photographic plates he found that the uranium emitted radiation. Becquerel had discovered radioactivity. Models of the Atom Ernest Rutherford, A
Study Guide A. Answer Key. Cell Growth and Division. SECTION 1. THE CELL CYCLE 1. a; d; b; c 2. gaps 3. c and d 4. c 5. b and d 6.
Cell Growth and Division Answer Key SECTION 1. THE CELL CYCLE 1. a; d; b; c 2. gaps 3. c and d 4. c 5. b and d 6. G 1 7. G 0 8. c 9. faster; too large 10. volume 11. a and b 12. repeating pattern or repetition
Colorimetric GAPDH Assay Cat. No. 8148, 100 tests
Colorimetric GAPDH Assay Cat. No. 8148, 1 tests Introduction Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) is a tetrameric enzyme that catalyzes glycolysis and thus serves to break down glucose for
Wallace Hall Academy Physics Department. Radiation. Pupil Notes Name:
Wallace Hall Academy Physics Department Radiation Pupil Notes Name: Learning intentions for this unit? Be able to draw and label a diagram of an atom Be able to state what alpha particles, beta particles
Chapter 10. Mitosis and Cytokinesis
Chapter 10. Mitosis and Cytokinesis Mitosis is nuclear division. In the process daughter molecules of DNA are precisely segregated into two new daughter nuclei. Mitosis is usually associated with cell
Introduction. Key Concepts I: Mitosis. AP Biology Laboratory 3 Mitosis & Meiosis
Virtual Student Guide http://www.phschool.com/science/biology_place/labbench/index.html AP Biology Laboratory 3 Mitosis & Meiosis Introduction For organisms to grow and reproduce, cells must divide. Mitosis
What happens during nuclear decay? During nuclear decay, atoms of one element can change into atoms of a different element altogether.
When Henri Becquerel placed uranium salts on a photographic plate and then developed the plate, he found a foggy image. The image was caused by rays that had not been observed before. For his discovery
AP Biology. Biology is the only subject in which multiplication is the same thing as division. The Cell Cycle: Cell Growth, Cell Division
QuickTime and and a TIFF TIFF (Uncompressed) decompressor are are needed needed to to see see this this picture. picture. Biology is the only subject in which multiplication is the same thing as division
Cell Structure and Cell Cycle
E X E R C I S E 4 Cell Structure and Cell Cycle Materials model or diagram of a cell compound microscopes and lens paper prepared slides of human skeletal muscle cells, pseudostratified ciliated columnar
Three different fusions led to three basic ideas: 1) If one fuses a cell in mitosis with a cell in any other stage of the cell cycle, the chromosomes
Section Notes The cell division cycle presents an interesting system to study because growth and division must be carefully coordinated. For many cells it is important that it reaches the correct size
Investigation 7 Part 1: CELL DIVISION: MITOSIS
Investigation 7 Part 1: CELL DIVISION: MITOSIS How do eukaryotic cells divide to produce genetically identical cells? BACKGROUND One of the characteristics of living things is the ability to replicate
M1. (a) (i) cannot penetrate aluminium allow can only pass through air / paper too weak is neutral 1
M. (a) (i) cannot penetrate aluminium allow can only pass through air / paper too weak is neutral gamma rays not affected (by aluminium) allow all / most (gamma rays) to pass through too strong is neutral
4th Six Weeks Exam Review Cell Structure and Function
Mrs. Keadle H Science 4th Six Weeks Exam Review Questions for your 4th six weeks exam will be similar to these questions. Make sure you understand the question and why the answer reviewed is correct. The
AS Biology Summer Work 2015
AS Biology Summer Work 2015 You will be following the OCR Biology A course and in preparation for this you are required to do the following for September 2015: Activity to complete Date done Purchased
Answer Key. Cell Growth and Division
Cell Growth and Division Answer Key SECTION 1. THE CELL CYCLE Cell Cycle: (1) Gap1 (G 1): cells grow, carry out normal functions, and copy their organelles. (2) Synthesis (S): cells replicate DNA. (3)
Quiz 1: Cells and Cell Structures
Quiz 1: Cells and Cell Structures 1. Identify the structures in the diagram. (3 marks) 2. List the 3 cell structures not found in animal cells but are found in plants cells. (1 mark) 3. Where is DNA found
Plant and animal cells (eukaryotic cells) have a cell membrane, cytoplasm and genetic material enclosed in a nucleus.
4.1 Cell biology Cells are the basic unit of all forms of life. In this section we explore how structural differences between types of cells enables them to perform specific functions within the organism.
Mitosis & Meiosis Practice Test
Name: DO NOT WRITE ON THIS TEST Class: ALL ID: A Mitosis & Meiosis Practice Test Modified True/False Indicate whether the statement is true or false. If false, change the identified word or phrase to make
LESSON 2.2 WORKBOOK. How is a cell born? Workbook Lesson 2.2
For a complete list of defined terms, see the Glossary. Cell cycle the progression of events that prepares a cell to replicate, and then leads to division into two daughter cells. Mitosis the phase of
BIOLOGY. Chapter 10 CELL REPRODUCTION PowerPoint Image Slideshow
BIOLOGY Chapter 10 CELL REPRODUCTION PowerPoint Image Slideshow FIGURE 10.1 A sea urchin begins life as a single cell that (a) divides to form two cells, visible by scanning electron microscopy. After
RADIOACTIVITY IN THE AIR
RADIOACTIVITY IN THE AIR REFERENCES M. Sternheim and J. Kane, General Physics (See the discussion on Half Life) Evans, The Atomic Nucleus, pp. 518-522 Segre, Nuclei and Particles, p. 156 See HEALTH AND
GraspIT AQA GCSE Cell Biology
A. Cell structure part 1 Eukaryotes, prokaryotes and animal and plant cells 1. Describe the similarities and differences between a typical plant and a typical animal cell. (4)...... 2. Ribosomes synthesise
Isotopes of an element have the same symbol and same atomic number - Mass number refers to the protons plus neutrons in an isotope
7.1 Atomic Theory and Radioactive Decay Natural background radiation exists all around us. This radiation consists of high energy particles or waves being emitted from a variety of materials Radioactivity
Cell Division (Outline)
Cell Division (Outline) 1. Overview of purpose and roles. Comparison of prokaryotic and eukaryotic chromosomes and relation between organelles and cell division. 2. Eukaryotic cell reproduction: asexual
The detector and counter are used in an experiment to show that a radioactive source gives out alpha and beta radiation only.
ATOMS AND NUCLEAR RADIATION PART II Q1. The detector and counter are used in an experiment to show that a radioactive source gives out alpha and beta radiation only. Two different types of absorber are
Unit 6 Nuclear Radiation Parent Guide. What is radioactivity and why are things radioactive?
Unit 6 Nuclear Radiation Parent Guide What is radioactivity and why are things radioactive? The nucleus of an atom is comprised of subatomic particles called protons and neutrons. Protons have a positive
Previous Class. Today. Reasons for analyzing pre-steady state conditions Methods for pre-steady state measurements
Previous Class Reasons for analyzing pre-steady state conditions Methods for pre-steady state measurements Today Practical Methods for Kinetics and Equilibria Spectrophotometry Radioactive Procedures Spectrofluorimetry
Initiation of DNA Replication Lecture 3! Linda Bloom! Office: ARB R3-165! phone: !
Initiation of DNA Replication Lecture 3! Linda Bloom! Office: ARB R3-165! email: lbloom@ufl.edu! phone: 392-8708! 1! Lecture 3 Outline! Students will learn! Basic techniques for visualizing replicating
Lab 7: Mitosis and the Cell Cycle
Lab 7: Mitosis and the Cell Cycle Each cell has a limited number of options for its future: (1) grow and divide (though this can be delayed in some cells, such as primary oocytes) (2) differentiate into
Fertilization of sperm and egg produces offspring
In sexual reproduction Fertilization of sperm and egg produces offspring In asexual reproduction Offspring are produced by a single parent, without the participation of sperm and egg CONNECTIONS BETWEEN
Honors Biology-CW/HW Cell Biology 2018
Class: Date: Honors Biology-CW/HW Cell Biology 2018 Multiple Choice Identify the choice that best completes the statement or answers the question. 1. Hooke s discovery of cells was made observing a. living
Nonlinear Optics. Single-Molecule Microscopy Group. Physical Optics Maria Dienerowitz.
Single-Molecule Microscopy Group Nonlinear Optics Physical Optics 21-06-2017 Maria Dienerowitz maria.dienerowitz@med.uni-jena.de www.single-molecule-microscopy.uniklinikum-jena.de Contents Introduction
CELL PRACTICE TEST
Name: Date: 1. As a human red blood cell matures, it loses its nucleus. As a result of this loss, a mature red blood cell lacks the ability to (1) take in material from the blood (2) release hormones to
Name 8 Cell Cycle and Meiosis Test Date Study Guide You must know: The structure of the replicated chromosome. The stages of mitosis.
Name 8 Cell Cycle and Meiosis Test Date Study Guide You must know: The structure of the replicated chromosome. The stages of mitosis. The role of kinases and cyclin in the regulation of the cell cycle.
NucView TM 488 Caspase-3 Assay Kit for Live Cells
NucView TM 488 Caspase-3 Assay Kit for Live Cells Catalog Number: 30029 (100-500 assays) Contact Information Address: Biotium, Inc. 3423 Investment Blvd. Suite 8 Hayward, CA 94545 USA Telephone: (510)
THE REACTIVATION OF THE RED CELL NUCLEUS
J. Cell Sci. 2, 23-32 (1967) 23 Printed in Great Britain THE REACTIVATION OF THE RED CELL NUCLEUS H.HARRIS The Sir William Dunn School of Pathology, University of Oxford SUMMARY When the nucleus of a mature
State variables and processes. Why do we want to measure processes? G: Bacterial Growth Rate (gc h -1 ) U DOM. Other compounds (e.g.
acterial Production Lab State variables and processes U DOM State Var. Process G: acterial Growth Rate (gc h - ) CO 2 Other compounds (e.g., EtOH) Objective: Measure bacterial growth rate (also called
Assessment Schedule 2017 Biology: Demonstrate understanding of life processes at the cellular level (91156)
NCEA Level 2 Biology (91156) 2017 page 1 of 6 Assessment Schedule 2017 Biology: Demonstrate understanding of life processes at the cellular level (91156) Assessment Criteria Achievement Achievement with
PS-21 First Spring Institute say : Teaching Physical Science. Radioactivity
PS-21 First Spring Institute say 2012-2013: Teaching Physical Science Radioactivity What Is Radioactivity? Radioactivity is the release of tiny, highenergy particles or gamma rays from the nucleus of an
Ch05. Radiation. Energy and matter that comes from the nucleus of an atom. version 1.6
Ch05 Radiation Energy and matter that comes from the nucleus of an atom. version 1.6 Nick DeMello, PhD. 2007-2016 Ch05 Radiation The Discovery of Radioactivity Phosphorescence Radioactive history Antoine
FREEMAN MEDIA INTEGRATION GUIDE Chapter 7: Inside the Cell
FREEMAN MEDIA INTEGRATION GUIDE Chapter 7: Inside the Cell All media is on the Instructors Resource CD/DVD JPEG Resources Figures, Photos, and Tables PowerPoint Resources Chapter Outline with Figures Lecture
How many lessons is it?
Science Unit Learning Summary Content Eukaryotes and Prokaryotes Cells are the basic unit of all life forms. A eukaryotic cell contains genetic material enclosed within a nucleus. Plant and animal cells
Chapter 4 and Chapter 5. Chapter 5
Chapter 4 and Chapter 5 Summary Chapter 4 The nucleus controls the functions of life. Chromosomes found within the nucleus contain the genes that store the information to make proteins. (4.1) Genetic information
3.2.2 All cells arise from other cells
alevelbiology.co.uk SPECIFICATION Within multicellular organisms, not all cells retain the ability to divide. Eukaryotic cells that do retain the ability to divide show a cell cycle. DNA replication occurs
Name # Class Date Regents Review: Cells & Cell Transport
Name # Class Date Regents Review: Cells & Cell Transport 1. All of the following are true regarding cells except? A) All cells have genetic material B) All cells have cell walls C) All cells have plasma
Nuclear Chemistry. Proposal: build a nuclear power plant in Broome County. List the pros & cons
Nuclear Chemistry Proposal: build a nuclear power plant in Broome County. List the pros & cons 1 Nuclear Chemistry Friend or Fiend 2 The Nucleus What is in the nucleus? How big is it vs. the atom? How
Chapter 10. Section 10.1 What is Radioactivity?
Chapter 10 Section 10.1 What is Radioactivity? What happens when an element undergoes radioactive decay? How does radiation affect the nucleus of an unstable isotope? How do scientists predict when an
MODULE 2 : FOUNDATIONS IN BIOLOGY
OCR A LEVEL BIOLOGY MODULE 2 : FOUNDATIONS IN BIOLOGY REVISION NOTES For 2015 onwards specification Miss T Banda All living things are primarily made from 4 key elements: Carbon (C) Hydrogen (H) Oxygen
Biology Notes 2. Mitosis vs Meiosis
Biology Notes 2 Mitosis vs Meiosis Diagram Booklet Cell Cycle (bottom corner draw cell in interphase) Mitosis Meiosis l Meiosis ll Cell Cycle Interphase Cell spends the majority of its life in this phase
O.k., Now Starts the Good Stuff (Part II) Eukaryotic Cell Structure and Function
O.k., Now Starts the Good Stuff (Part II) Eukaryotic Cell Structure and Function Eukaryotic Cells These cells have membrane-bound structures called organelles. Cell processes occur in these organelles.
Cell Biology. AQA Biology topic 1
Cell Biology AQA Biology topic 1 1.1 Cell Structure Plant and Animal cells (eukaryotic cells) Eukaryotic cells have these features: 1) Cytoplasm 2) Genetic material within a nucleus 3) Cell Membrane Typical