Methods in Enzymology. Volume 102. Hormone Action. Part G Calmodulin and Calcium-Binding Protein s. Anthony R. Means. Bert W.

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1 Methods in Enzymology Volume 102 Hormone Action Part G Calmodulin and Calcium-Binding Protein s Anthony R. Means Bert W. O'Malley

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3 CONTRIBUTORS TO VOLUME 102 PREFACE VOLUMES IN SERIES ix xiii XV 1. Calmodulin Purification and Fluorescent Labeling JOHN R. DEDMAN AN D MARCIA A. KAETZEL 1 2. Purification of Plant Calmodulin JAMES MICHAEL ANDERSON 9 3. Purification of Calmodulin by Ca z+ -Dependent Af- HARRY CHARBONNEAU, finity Chromatography RITA RICE, RUSSELL C. HART, AND MILTON J. CORMIER Assay of Calmodulin by Cat+-Dependent Phos- ROBERT W. WALLACE, phodiesterase E. ANN TALLANT, AN D WAI YIU CHEUNG The Red Blood Cell as a Model for Calmodulin- THOMAS R. HINDS AN D Dependent Ca z+ Transport FRANK F. VINCENZI Myosin Light Chain Phosphorylation in Smooth PAUL J. SILVER AND Muscle and Nonmuscle Cells as a Probe of JAMES T. STULL 62 Calmodulin Functio n 7. Assessment of Caz+-Calmodulin Formation in In- DAVID R. MANNING AN D tact Vertebrate Skeletal Muscle JAMES T. STULL Spectroscopic Analyses of Calmodulin and Its In- RACHEL E. KLEVIT 82 teraction s 9. Production of Polyclonal and Monoclonal Anti- JAMES G. CHAFOULEAS, bodies to Calmodulin and Utilization of These MARY E. RISER, Immunological Probes LISETTE LAGAC@, AND ANTHONY R. MEANS Localization of Calmodulin and Calmodulin Ac- MICHAEL J. WELSH 11 0 ceptor Sites by Fluorescence Method s 11. Immunofluorescence Localization of Calmodulin JEFFREY F. HARPER AN D in Unfixed Frozen Tissue Sections ALTON L. STEINER Caz+ Binding to Calmodulin JAMES D. POTTER, PRISCILLA STRANG-BROWN, PATRICIA L. WALKER, AN D SHOZO IIDA Preparation of Calmodulin Crystals WILLIAM J. COOK AN D JOHN S. SACK 143

4 14. Preparation of Fluorescent Labeled Calmodulins BRADLEY B. OLwIN AND DANIEL R. STORM Posttranslational Modification of Calmodulin TIMOTHY J. MURTAUGH, PAUL M. RowE, PAMELA L. VINCENT, LYNDA S. WRIGHT, AN D FRANK L. SIEGEL Techniques for Measuring the Interaction of BENJAMIN WEISS 17 1 Drugs with Calmoduli n 17. Naphthalenesulfonamides as Calmodulin Antago- HIROYOSHI HIDAKA AN D nists TOSHIO TANAKA Synthesis and Characterization of Calmodulin An- RussELL C. HART, tagonistic Drugs MICHAEL D. BATES, MILTON J. CORMIER, GERALD M. ROSEN, AN D P. MICHAEL CONN Detection of Calmodulin-Binding Polypeptides JOHN R. GLENNEY, JR. AN D Separated in SDS-Polyacrylamide Gels by a KLAUS WEBER 204 Sensitive [ 1251]Calmodulin Gel Overlay Assay 20. Use of Calmodulin Affinity Chromatography for RAJENDRA K. SHARMA, Purification of Specific Calmodulin-Dependent WILLIAM A. TAYLOR, AND Enzymes JERRY H. WANG Calmodulin as an Integral Subunit of Phosphory- COLIN PICTON, lase Kinase from Rabbit Skeletal Muscle SHIRISH SHENOLIKAR, ROGER GRAND, AN D PHILIP COHEN Isolation and Characterization of Bovine Brain C. B. KLEE, Calcineurin : A Calmodulin-Stimulated Protein M. H. KRINKS, Phosphatase A. S. MANALAN, P. COHEN, AND A. A. STEWART Purification and Radioimmunoassay of Calmodu- E. ANN TALLANT, lin-dependent Protein Phosphatase from Bo- ROBERT W. WALLACE, AN D vine Brain WAI YIU CHEUNG Assay of S-100 Protein by an Enzyme Immuno- HIROYOSHI HIDAKA, assay Method TOYOSHI ENDO, AN D KANEFUSA KATO Covalent Regulation of the Cardiac Sarcoplasmic CHRISTIAN J. LE PEUCH, Reticulum Calcium Pump : Purification and DANIELLE A.-M. LE PEUCH, Properties of Phospholamban, a Substrate of AND JACQUES G. D LE 26 1 camp-dependent Protein Kinase and Ca t+ - Calmodulin-Dependent Phospholamban Kinase

5 26. Cat+-Dependent Neutral Protease and Proteolytic NoRIO KAJIKAWA, Activation of Cat+-Activated, Phospholipid-De- AKIRA KISHIMOTO, pendent Protein Kinase MAKOTO SHIOTA, AN D YASUTOMI NISHIZUKA Enzyme-Linked Immunoabsorbent Assay BARBARA E. MILLER AN D (ELISA) and Radioimmunoassay (RIA) for the ANTHONY W. NORMAN 29 1 Vitamin D-Dependent 28,000 Dalton Calcium - Binding Protei n 28. Chemical Approaches to the Calmodulin System THOMAS C. VANAMAN 296 AUTHOR INDEX 31 1 SUBJECT INDEX 321