Introduction... Theory Influence of Excitation Pulse Shape...
|
|
|
- Isaac Norman
- 5 years ago
- Views:
Transcription
1 1. Fluorescence Anisotropy: Theory and Applications Robert F. Steiner Introduction... Theory Meaning of Anisotropy Influence of Excitation Pulse Shape The Time Decay of Anisotropy The Rotational Diffusion of Ellipsoids of Revolution The Anisotropy Decay of Ellipsoidal Particles Partially Immobilized Systems The Influence of Internal Rotation... Experimental Analysis of Anisotropy Decay Analysis of Time-Domain Data Time-Domain Measurements of Anisotropy Decay Frequency-Domain Measurements of Anisotropy Decay... Anisotropy Decay of Heterogeneous Systems Anisotropy-Resolved Emission Spectra... The Meaning of Correlation Times for Associative and Nonassociative Heterogeneity... Anisotropy Decay of Intrinsic Protein Fluorophores Anisotropy Decay of a Rigid Protein: S. Nuclease... Rotational Dynamics of Flexible Polypeptides: Adrenocorticotropin and Melittin Anisotropy Decay of a Tightly Bound Fluorophore: Lumazine Protein Anisotropy Decay of a Transfer RNA... Anisotropy Decay of Biopolymers Labeled with an Extrinsic Fluorophore Anisotropy Decay and Internal Flexibility of Myosin... Anisotropy Decay of a Fibrous Protein: F-Actin... Anisotropy Decay for Proteins Displaying Internal Rotation Involving a Well-Defined Domain: The Immunoglobulins ix
2 x Contents Anisotropy Decay of Calmodulin Complexes with TNS... References Fluorescence Quenching: Theory and Applications Maurice R. Eftink Introduction... Basic Concepts The Stern Volmer Equation Quenching Mechanisms and Efficiency... Diffusional Nature of Quenching... Static Quenching Various Quenchers... Quenching Studies with Proteins Exposure of Fluorophores Effect of the Macromolecule s Size Electrostatic Effects Tryptophan Residues in Proteins Ligand Binding and Conformational Changes... Mechanism of Quenching in Proteins Penetration versus Unfolding Mechanisms Interaction of Quenchers with Proteins Transient Effects Multiple Quenching Rate Constants and Fluorescence Lifetimes... Studies with Membranes and Nucleic Acids Partitioning of Quenchers into Membranes/Micelles Two-Dimensional Diffusion in Membranes Quencher Moieties Attached to Lipid Molecules Membrane Transport and Surface Potential Nucleic Acids... Uses to Resolve Other Fluorescence Properties Resolution of Steady-State Spectra Resolution of Fluorescence Lifetimes Resolution of Anisotropy Measurements Resolution of Energy Transfer Experiments Other Uses of Solute Quenching.... Recent Developments in Data Analysis Simultaneous Analyses of Quenching Data... Nonlinear Least-Squares Fits... Distribution of Lifetimes or Rate Constants... Experimental Improvements
3 Phosphorescence Quenching... Conclusion... References... xi Resonance Energy Transfer Herbert C. Cheung Long-Range Dipole Dipole Interaction... Determination of Energy Transfer... Proximity Mapping of Molecular Assembly... Experimental Strategy Sample Preparation Measurement of Transfer Efficiency The Orientation Factor... Selected Applications Myosin and Actomyosin Troponin Subunits Ribosomal Proteins... Comparison of FRET Results with Results from Other Techniques Comparison with Crystallographic Data Comparison with Cross-Linking Data... Application of FRET to Enzyme Kinetics... Time-Resolved Energy Transfer Measurements... Distribution of Distances Theory Examples Summary and Prospects... References Least-Squares Analysis of Fluorescence Data Martin Straume, Susan G. Frasier-Cadoret, and Michael L. Johnson Introduction... Basic Terminology... Assumptions of Least-Squares Analysis... Least-Squares Parameter Estimation Procedures Modified Gauss Newton Algorithm Nelder Mead Simplex Algorithm... An Example of the Least-Squares Procedures Collisional Quenching
4 xii Contents Example of the Gauss Newton Procedure Example of the Nelder Mead Simplex Procedure Joint Confidence Intervals Estimation and Propagation Asymptotic Standard Errors... Linear Joint Confidence Intervals... Support Plane Confidence Intervals... Approximate Nonlinear Support Plane Joint Confidence Intervals A Monte Carlo Method for the Evaluation of Confidence Intervals Propagation of Confidence Intervals... Analysis of Residuals Plots... Distributions... Trends... Outliers... Influential Observations... Common Quantitative Tests... Implementation Notes... In Summary... References The Global Analysis of Fluorescence Intensity and Anisotropy Decay Data: Second-Generation Theory and Programs Joseph M. Beechem, Enrico Gratton, Marcel Ameloot, Jay R. Knutson, and Ludwig Brand Introduction Multiexcitation/Multitemperature Studies of Anisotropic Rotation Multiexcitation/Emission Wavelength Studies of Total Intensity Data Double-Kinetic Studies... The Global Analysis Philosophy Evolution of the Global Analysis Approach Global Analysis Implementation Strategy... General Elements of the Global Analysis Program Mapping to the Physical Observables... Empirical Description of the Fluorescence Decay... Compartmental Description of Photophysical Events... Overview of Nonlinear Minimization (The Basic Equations)
5 xiii 5.4. In-Depth Flow Chart of a General-Purpose Global Analysis Program Overview of the Global Analysis Procedure Flow Chart for the LFD Global Analysis Program Global Case Studies of the Application of Global Analysis to Experimental Data Case Study of a Two-State Excited State Reaction Distributions of Distances and Energy Transfer Analysis Anisotropy Decay Data Analysis General Equations and Experimental Linkages... Changes in Anisotropy Data Collection Schemes... Associative versus Nonassociative Modeling of Anisotropy Anisotropy Decay-Associated Spectra (ADAS)... Multidye Global Anisotropy Decay Analysis... Distributed Lifetimes and Distributed Rotational Correlation Times Multiexcitation Anisotropy Experiments Example of Distributed Rotations: Fluorophore Rotations Gated by Packing Fluctuations in Lipid Bilayers... Error Analysis and the Identifiability Problem The Identifiability Problem... Identifiability Study Using Laplace Identifiability Analysis.. Error Analysis... Conclusions... References Fluorescence Polarization from Oriented Systems Thomas P. Burghardt and Katalin Ajtai Overview... Theory and Application The Angular Probability Density N... Fluorescence Polarization in Homogeneous Space... Time-Resolved Fluorescence Depolarization Determination of the High-Resolution Angular Probability Density... Relation of Electron Spin Resonance Spectra to Fluorescence Polarization... Biochemical Techniques of Specific Labeling Discussion... References
6 xiv Contents 7. Fluorescence-Based Fiber-Optic Sensors Richard B. Thompson Introduction... Fiber-Optic Fundamentals... Sensor Design... Sensing Tip Configurations... Fiber Characteristics... Separating Excitation and Emission... Launching Optics... Light Sources... Time-Resolved Fluorescence in Fibers... Polarization... Conclusion... References Inhomogeneous Broadening of Electronic Spectra of Dye Molecules in Solutions Nicolai A. Nemkovich, Anatolyi N. Rubinov, and Vladimir I. Tomin Introduction... Theoretical Considerations of Inhomogeneous Broadening Solvate Configurational Energy... Field Diagram of a Polar Solution Solvate Distribution in Configurational Sublevels Nonpolar Solutions Selective Excitation with Vibrational Spectral Broadening Absorption and Fluorescence Spectra: Dependence on Exciting Light Frequency... Stationary Inhomogeneous Broadening Universal Relationship between Fluorescence and Absorption Spectra of Polar Solutions Luminescence Spectra at Red-Edge Excitation Directed Nonradiative Energy Transfer in Organic Solutions Dynamic Inhomogeneous Broadening in Liquid Solutions Analysis of Configurational Relaxation in Liquid Solutions Experimental Study of the Luminescence Kinetics of Liquid Solutions... The Solution Spectrochronogram... The Effect of Light-Induced Molecular Rotation in Solution
7 xv Selective Kinetic Spectroscopy of Fluorescent Molecules in Phospholipid Membranes Energy Levels of an Electric Dipole Probe in a Membrane Inhomogeneous Broadening in Steady-State Fluorescence Spectra of Probes... Kinetics of Probe Fluorescence... Rotational Dynamics of the Probe in the Membrane Conclusions... References Index
Contents. xiii. Preface v
Contents Preface Chapter 1 Biological Macromolecules 1.1 General PrincipIes 1.1.1 Macrornolecules 1.2 1.1.2 Configuration and Conformation Molecular lnteractions in Macromolecular Structures 1.2.1 Weak
Fluorescence (Notes 16)
Fluorescence - 2014 (Notes 16) XV 74 Jablonski diagram Where does the energy go? Can be viewed like multistep kinetic pathway 1) Excite system through A Absorbance S 0 S n Excite from ground excited singlet
Lecture 5. Anisotropy decay/data analysis. Enrico Gratton
Lecture 5. Anisotropy decay/data analysis Enrico Gratton Anisotropy decay Energy-transfer distance distributions Time resolved spectra Excited-state reactions Basic physics concept in polarization The
Single-Molecule Methods I - in vitro
Single-Molecule Methods I - in vitro Bo Huang Macromolecules 2014.03.10 F 1 -ATPase: a case study Membrane ADP ATP Rotation of the axle when hydrolyzing ATP Kinosita group, 1997-2005 Single Molecule Methods
ATOMIC AND LASER SPECTROSCOPY
ALAN CORNEY ATOMIC AND LASER SPECTROSCOPY CLARENDON PRESS OXFORD 1977 Contents 1. INTRODUCTION 1.1. Planck's radiation law. 1 1.2. The photoelectric effect 4 1.3. Early atomic spectroscopy 5 1.4. The postulates
CHEM Outline (Part 15) - Luminescence 2013
CHEM 524 -- Outline (Part 15) - Luminescence 2013 XI. Molecular Luminescence Spectra (Chapter 15) Kinetic process, competing pathways fluorescence, phosphorescence, non-radiative decay Jablonski diagram
Fluorescence 2009 update
XV 74 Fluorescence 2009 update Jablonski diagram Where does the energy go? Can be viewed like multistep kinetic pathway 1) Excite system through A Absorbance S 0 S n Excite from ground excited singlet
CD Basis Set of Spectra that is used is that derived from comparing the spectra of globular proteins whose secondary structures are known from X-ray
CD Basis Set of Spectra that is used is that derived from comparing the spectra of globular proteins whose secondary structures are known from X-ray crystallography An example of the use of CD Modeling
single-molecule fluorescence resonance energy transfer
single-molecule fluorescence resonance energy transfer (2) determing the Förster radius: quantum yield, donor lifetime, spectral overlap, anisotropy michael börsch 26/05/2004 1 fluorescence (1) absorbance
Principles of Physical Biochemistry
Principles of Physical Biochemistry Kensal E. van Hold e W. Curtis Johnso n P. Shing Ho Preface x i PART 1 MACROMOLECULAR STRUCTURE AND DYNAMICS 1 1 Biological Macromolecules 2 1.1 General Principles
Fundamentals of Spectroscopy for Optical Remote Sensing. Course Outline 2009
Fundamentals of Spectroscopy for Optical Remote Sensing Course Outline 2009 Part I. Fundamentals of Quantum Mechanics Chapter 1. Concepts of Quantum and Experimental Facts 1.1. Blackbody Radiation and
Fluorescence Depolarization. Ben Berger and Ben Berger
Fluorescence Depolarization Ben Berger and Ben Berger Small molecules rotate quickly Large molecules rotate slowly There is a time delay from the moment a molecule is excited to the time it fluoresces
Luminescence spectroscopy
Febr. 203 Luminescence spectroscopy Biophysics 2 nd semester Józse Orbán University o Pécs, Department o Biophysics Deinitions, laws FUNDAMENTALS o SPECTROSCY review - Spectral types (absorbtion/emission
PROTEIN NMR SPECTROSCOPY
List of Figures List of Tables xvii xxvi 1. NMR SPECTROSCOPY 1 1.1 Introduction to NMR Spectroscopy 2 1.2 One Dimensional NMR Spectroscopy 3 1.2.1 Classical Description of NMR Spectroscopy 3 1.2.2 Nuclear
Fluorescence Polarization Anisotropy FPA
Fluorescence Polarization Anisotropy FPA Optics study of light Spectroscopy = light interacts the study of the interaction between matter & electro-magnetic radiation matter Spectroscopy Atomic Spectroscopy
Modern Optical Spectroscopy
Modern Optical Spectroscopy With Exercises and Examples from Biophysics and Biochemistry von William W Parson 1. Auflage Springer-Verlag Berlin Heidelberg 2006 Verlag C.H. Beck im Internet: www.beck.de
LABORATORY OF ELEMENTARY BIOPHYSICS
LABORATORY OF ELEMENTARY BIOPHYSICS Experimental exercises for III year of the First cycle studies Field: Applications of physics in biology and medicine Specialization: Molecular Biophysics Fluorescence
BMB Class 17, November 30, Single Molecule Biophysics (II)
BMB 178 2018 Class 17, November 30, 2018 15. Single Molecule Biophysics (II) New Advances in Single Molecule Techniques Atomic Force Microscopy Single Molecule Manipulation - optical traps and tweezers
XV 74. Flouorescence-Polarization-Circular-Dichroism- Jablonski diagram Where does the energy go?
XV 74 Flouorescence-Polarization-Circular-Dichroism- Jablonski diagram Where does the energy go? 1) Excite system through A Absorbance S 0 S n Excite from ground excited singlet S = 0 could be any of them
MOLECULAR SPECTROSCOPY
MOLECULAR SPECTROSCOPY First Edition Jeanne L. McHale University of Idaho PRENTICE HALL, Upper Saddle River, New Jersey 07458 CONTENTS PREFACE xiii 1 INTRODUCTION AND REVIEW 1 1.1 Historical Perspective
FLUORESCENCE STUDY ON TRYPTOPHAN POTASSIUM IODIDE INTERACTION
PROCEEDINGS OF THE YEREVAN STATE UNIVERSITY C h e m i s t r y a n d B i o l o g y 08, 5(), p. 75 79 FLUORESCENCE STUDY ON TRYPTOPHAN POTASSIUM IODIDE INTERACTION C h emistr y H. A. SHILAJYAN, K. R. GRIGORYAN
Linear and nonlinear spectroscopy
Linear and nonlinear spectroscopy We ve seen that we can determine molecular frequencies and dephasing rates (for electronic, vibrational, or spin degrees of freedom) from frequency-domain or timedomain
Spectroscopy of Polymers
Spectroscopy of Polymers Jack L. Koenig Case Western Reserve University WOMACS Professional Reference Book American Chemical Society, Washington, DC 1992 Contents Preface m xiii Theory of Polymer Characterization
Rotational Brownian motion; Fluorescence correlation spectroscpy; Photobleaching and FRET. David A. Case Rutgers, Spring 2009
Rotational Brownian motion; Fluorescence correlation spectroscpy; Photobleaching and FRET David A. Case Rutgers, Spring 2009 Techniques based on rotational motion What we studied last time probed translational
SECOND PUBLIC EXAMINATION. Honour School of Physics Part C: 4 Year Course. Honour School of Physics and Philosophy Part C C7: BIOLOGICAL PHYSICS
2757 SECOND PUBLIC EXAMINATION Honour School of Physics Part C: 4 Year Course Honour School of Physics and Philosophy Part C C7: BIOLOGICAL PHYSICS TRINITY TERM 2013 Monday, 17 June, 2.30 pm 5.45 pm 15
1. Transition dipole moment
1. Transition dipole moment You have measured absorption spectra of aqueous (n=1.33) solutions of two different chromophores (A and B). The concentrations of the solutions were the same. The absorption
Medical Biophysics II. Final exam theoretical questions 2013.
Medical Biophysics II. Final exam theoretical questions 2013. 1. Early atomic models. Rutherford-experiment. Franck-Hertz experiment. Bohr model of atom. 2. Quantum mechanical atomic model. Quantum numbers.
Fluorescence Workshop UMN Physics June 8-10, 2006 Basic Spectroscopic Principles Joachim Mueller
Fluorescence Workshop UMN Physics June 8-10, 2006 Basic Spectroscopic Principles Joachim Mueller Fluorescence, Light, Absorption, Jablonski Diagram, and Beer-Law First stab at a definition: What is fluorescence?
Singlet. Fluorescence Spectroscopy * LUMO
Fluorescence Spectroscopy Light can be absorbed and re-emitted by matter luminescence (photo-luminescence). There are two types of luminescence, in this discussion: fluorescence and phosphorescence. A
Spin Dynamics Basics of Nuclear Magnetic Resonance. Malcolm H. Levitt
Spin Dynamics Basics of Nuclear Magnetic Resonance Second edition Malcolm H. Levitt The University of Southampton, UK John Wiley &. Sons, Ltd Preface xxi Preface to the First Edition xxiii Introduction
Direct, Reliable, and Correct Oxygen Measurement
Direct, Reliable, and Correct Oxygen Measurement February, 2015 Oxygen-dependent quenching of phosphorescence has been used extensively for the last 25 years as a reliable method for measuring oxygen.
Chemistry 524--Final Exam--Keiderling May 4, :30 -?? pm SES
Chemistry 524--Final Exam--Keiderling May 4, 2011 3:30 -?? pm -- 4286 SES Please answer all questions in the answer book provided. Calculators, rulers, pens and pencils are permitted. No open books or
Experimental Physical Chemistry : A Laboratory Textbook. ix To the Student Part 1 Fundamentals: Data Collection and
Experimental Physical Chemistry : A Laboratory Textbook Table Of Contents: Preface ix To the Student xv Part 1 Fundamentals: Data Collection and 1 (39) Analysis Working with Experimental Data 1 (50) Collecting
Wolfgang Demtroder. Laser Spectroscopy. Basic Concepts and Instrumentation. Second Enlarged Edition With 644 Figures and 91 Problems.
Wolfgang Demtroder Laser Spectroscopy Basic Concepts and Instrumentation Second Enlarged Edition With 644 Figures and 91 Problems Springer Contents 1. Introduction 1 2. Absorption and Emission of Light
Fluorescence polarisation, anisotropy FRAP
Fluorescence polarisation, anisotropy FRAP Reminder: fluorescence spectra Definitions! a. Emission sp. b. Excitation sp. Stokes-shift The difference (measured in nm) between the peak of the excitation
4 Single molecule FRET
4 Single molecule FRET FRET basics Energie Dipole-dipole interaction Teil I SM Fluo, Kap. 4 FRET FRET basics transfer rate (from Fermis Golden Rule) k t = 1 0 1 r 6 apple 2 9 ln(10) n 4 N A 128 5 Z d f
Laser Physics OXFORD UNIVERSITY PRESS SIMON HOOKER COLIN WEBB. and. Department of Physics, University of Oxford
Laser Physics SIMON HOOKER and COLIN WEBB Department of Physics, University of Oxford OXFORD UNIVERSITY PRESS Contents 1 Introduction 1.1 The laser 1.2 Electromagnetic radiation in a closed cavity 1.2.1
Discussion Session prior to the Second Examination: Sunday evening April 13 6 to 8 pm. 161 Noyes Laboratory
Discussion Session prior to the Second Examination: Sunday evening April 13 6 to 8 pm 161 Noyes Laboratory Determination of the Stokes Radius by measuring the Rotational Diffusion Coefficient: D rot D
Supplemental Materials and Methods
Supplemental Materials and Methods Time-resolved FRET (trfret) to probe for changes in the Box A/A stem upon complex assembly U3 MINI was folded and the decay of Fl fluorescence was measured at 20 ºC (see
PRINCIPLES OF NONLINEAR OPTICAL SPECTROSCOPY
PRINCIPLES OF NONLINEAR OPTICAL SPECTROSCOPY Shaul Mukamel University of Rochester Rochester, New York New York Oxford OXFORD UNIVERSITY PRESS 1995 Contents 1. Introduction 3 Linear versus Nonlinear Spectroscopy
Luminescence Spectroscopy Excitation is very rapid (10-15 s). Vibrational relaxation is a non-radiational process. It involves vibrational levels of
Luminescence Spectroscopy Excitation is very rapid (10-15 s). Vibrational relaxation is a non-radiational process. It involves vibrational levels of the same electronic state. The excess of vibrational
P. Lambrev October 10, 2018
TIME-RESOLVED OPTICAL SPECTROSCOPY Petar Lambrev Laboratory of Photosynthetic Membranes Institute of Plant Biology The Essence of Spectroscopy spectro-scopy: seeing the ghosts of molecules Kirchhoff s
Nanoscale confinement of photon and electron
Nanoscale confinement of photon and electron Photons can be confined via: Planar waveguides or microcavities (2 d) Optical fibers (1 d) Micro/nano spheres (0 d) Electrons can be confined via: Quantum well
Elements of Quantum Optics
Pierre Meystre Murray Sargent III Elements of Quantum Optics Fourth Edition With 124 Figures fya Springer Contents 1 Classical Electromagnetic Fields 1 1.1 Maxwell's Equations in a Vacuum 2 1.2 Maxwell's
Fluorescence Workshop UMN Physics June 8-10, 2006 Quantum Yield and Polarization (1) Joachim Mueller
Fluorescence Workshop UMN Physics June 8-10, 2006 Quantum Yield and Polarization (1) Joachim Mueller Quantum yield, polarized light, dipole moment, photoselection, dipole radiation, polarization and anisotropy
THE NUCLEAR OVERHAUSER EFFECT IN STRUCTURAL AND CONFORMATIONAL ANALYSIS
THE NUCLEAR OVERHAUSER EFFECT IN STRUCTURAL AND CONFORMATIONAL ANALYSIS David Neuhaus and Michael P. Williamson VCH CONTENTS Preface v Acknowledgments vii Symbols, Abbreviations, and Units xvii Introduction
Sputtering by Particle Bombardment
Rainer Behrisch, Wolfgang Eckstein (Eds.) Sputtering by Particle Bombardment Experiments and Computer Calculations from Threshold to MeV Energies With 201 Figures e1 Springer Contents Introduction and
Near-Field Nano/Atom Optics and Technology
M. Ohtsu (Ed.) Near-Field Nano/Atom Optics and Technology With 189 Figures / Springer Preface List of Contributors V VII XIII 1. Introduction 1 1.1 Near-Field Optics and Related Technologies 1 1.2 History
Rotation and vibration of Molecules
Rotation and vibration of Molecules Overview of the two lectures... 2 General remarks on spectroscopy... 2 Beer-Lambert law for photoabsorption... 3 Einstein s coefficients... 4 Limits of resolution...
Electronic and Optoelectronic Properties of Semiconductor Structures
Electronic and Optoelectronic Properties of Semiconductor Structures Jasprit Singh University of Michigan, Ann Arbor CAMBRIDGE UNIVERSITY PRESS CONTENTS PREFACE INTRODUCTION xiii xiv 1.1 SURVEY OF ADVANCES
What the Einstein Relations Tell Us
What the Einstein Relations Tell Us 1. The rate of spontaneous emission A21 is proportional to υ 3. At higher frequencies A21 >> B(υ) and all emission is spontaneous. A 21 = 8π hν3 c 3 B(ν) 2. Although
TIME-RESOLVED OPTICAL SPECTROSCOPY
TIME-RESOLVED OPTICAL SPECTROSCOPY Petar Lambrev Laboratory of Photosynthetic Membranes Institute of Plant Biology The Essence of Spectroscopy spectro-scopy: seeing the ghosts of molecules Kirchhoff s
Protein dynamics from NMR Relaxation data
Protein dynamics from NMR Relaxation data Clubb 3/15/17 (S f2 ) ( e ) Nitrogen-15 relaxation ZZ-exchange R 1 = 1/T 1 Longitudinal relaxation (decay back to z-axis) R 2 = 1/T 2 Spin-spin relaxation (dephasing
Three-Dimensional Electron Microscopy of Macromolecular Assemblies
Three-Dimensional Electron Microscopy of Macromolecular Assemblies Joachim Frank Wadsworth Center for Laboratories and Research State of New York Department of Health The Governor Nelson A. Rockefeller
Survey on Laser Spectroscopic Techniques for Condensed Matter
Survey on Laser Spectroscopic Techniques for Condensed Matter Coherent Radiation Sources for Small Laboratories CW: Tunability: IR Visible Linewidth: 1 Hz Power: μw 10W Pulsed: Tunabality: THz Soft X-ray
Measurement Examples. Excitation and Emission Scans. Steady State Fluorescence Anisotropy. Kinetic Measurements
Measurement Examples A division of Edinburgh Instruments Ltd. Excitation and Emission Scans Excitation and emission spectra are standard measurements in fluorescence spectroscopy. The figure demonstrates
THEORY OF MAGNETIC RESONANCE
THEORY OF MAGNETIC RESONANCE Second Edition Charles P. Poole, Jr., and Horacio A. Farach Department of Physics University of South Carolina, Columbia A Wiley-lnterscience Publication JOHN WILEY & SONS
Chapter 15 Molecular Luminescence Spectrometry
Chapter 15 Molecular Luminescence Spectrometry Two types of Luminescence methods are: 1) Photoluminescence, Light is directed onto a sample, where it is absorbed and imparts excess energy into the material
Novel Nanoparticles for Ultrasensitive Detection and Spectroscopy
Final Technical Report (DOE-FG02-98ER14873) Project Officer: Dr. Richard Gordon / Dr. John Miller Novel Nanoparticles for Ultrasensitive Detection and Spectroscopy Shuming Nie Indiana University P. 0.
Chemistry Instrumental Analysis Lecture 3. Chem 4631
Chemistry 4631 Instrumental Analysis Lecture 3 Quantum Transitions The energy of a photon can also be transferred to an elementary particle by adsorption if the energy of the photon exactly matches the
Self-Assembled Organic-Inorganic Nanostructures
Self-Assembled Organic-Inorganic Nanostructures Optics and Dynamics edited by Eduard Zenkevich Christian von Borczyskowski Self-Assembled Organic-Inorganic Nanostructures Self-Assembled Organic-Inorganic
Technology, Techniques and Applications. Ric Allott Business Development Manager
Technology, Techniques and Applications Ric Allott Business Development Manager 1 Central Laser Facility ASTRA GEMINI VULCAN ARTEMIS ULTRA OCTOPUS High power, ultrashort pulse dual beams of 15 J, 30 fs
Circular Dichroism. Principles and Applications VCH. Koji Nakanishi, Nina Berova, and Robert W. Woody EDITED BY
Circular Dichroism Principles and Applications EDITED BY Koji Nakanishi, Nina Berova, and Robert W. Woody VCH Contents Contributors xv Chapter 1 Circular Dichroism: An Introduction 1 Günther Snatzke 1.1
Magnetic Resonance Spectroscopy
INTRODUCTION TO Magnetic Resonance Spectroscopy ESR, NMR, NQR D. N. SATHYANARAYANA Formerly, Chairman Department of Inorganic and Physical Chemistry Indian Institute of Science, Bangalore % I.K. International
Spectroscopy. Practical Handbook of. J. W. Robinson, Ph.D., D.Sc, F.R.C.S. Department of Chemistry Louisiana State University Baton Rouge, Louisiana
Practical Handbook of Spectroscopy Edited by J. W. Robinson, Ph.D., D.Sc, F.R.C.S. Department of Chemistry Louisiana State University Baton Rouge, Louisiana CRC Press Boca Raton Ann Arbor Boston TABLE
INTRODUCTION TO MODERN VIBRATIONAL SPECTROSCOPY
INTRODUCTION TO MODERN VIBRATIONAL SPECTROSCOPY MAX DIEM Department of Chemistry City University of New York Hunter College A Wiley-Interscience Publication JOHN WILEY & SONS New York Chichester Brisbane
Application of time resolved area normalized emission spectroscopy to multicomponent systems
JOURNAL OF CHEMICAL PHYSICS VOLUME 115, NUMBER 15 15 OCTOBER 2001 Application of time resolved area normalized emission spectroscopy to multicomponent systems A. S. R. Koti and N. Periasamy a) Department
Fluorescence Excitation and Emission Fundamentals
Fluorescence Excitation and Emission Fundamentals Fluorescence is a member of the ubiquitous luminescence family of processes in which susceptible molecules emit light from electronically excited states
LIST OF TOPICS BASIC LASER PHYSICS. Preface xiii Units and Notation xv List of Symbols xvii
ate LIST OF TOPICS Preface xiii Units and Notation xv List of Symbols xvii BASIC LASER PHYSICS Chapter 1 An Introduction to Lasers 1.1 What Is a Laser? 2 1.2 Atomic Energy Levels and Spontaneous Emission
5 Recipient of Grant from the National Science Foundation. 11 Recipient of Grant PCM from the National Science
THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 259, No. 7, Issue of September 0, pp, 0967-0972,984 0 9% by Society The American of Biological Inc. Chemists, Printed In U. S A Determination of Time-resolved Fluorescence
OPTICAL PROPERTIES AND SPECTROSCOPY OF NANOAAATERIALS. Jin Zhong Zhang. World Scientific TECHNISCHE INFORMATIONSBIBLIOTHEK
OPTICAL PROPERTIES AND SPECTROSCOPY OF NANOAAATERIALS Jin Zhong Zhang University of California, Santa Cruz, USA TECHNISCHE INFORMATIONSBIBLIOTHEK Y World Scientific NEW JERSEY. t'on.don SINGAPORE «'BEIJING
CHAPTER 13 Molecular Spectroscopy 2: Electronic Transitions
CHAPTER 13 Molecular Spectroscopy 2: Electronic Transitions I. General Features of Electronic spectroscopy. A. Visible and ultraviolet photons excite electronic state transitions. ε photon = 120 to 1200
Protein-Ligand Interactions Are Responsible for Signal Transduction
Proteinigand Interactions Are Responsible for Signal ransduction ypes of Interactions: 1. ProteinProtein 2. ProteinDNA (RNA) 3. Proteinsmall molecule Dynamic Proteinigand Interaction Dynamic interaction
Model Answer (Paper code: AR-7112) M. Sc. (Physics) IV Semester Paper I: Laser Physics and Spectroscopy
Model Answer (Paper code: AR-7112) M. Sc. (Physics) IV Semester Paper I: Laser Physics and Spectroscopy Section I Q1. Answer (i) (b) (ii) (d) (iii) (c) (iv) (c) (v) (a) (vi) (b) (vii) (b) (viii) (a) (ix)
Wavelength λ Velocity v. Electric Field Strength Amplitude A. Time t or Distance x time for 1 λ to pass fixed point. # of λ passing per s ν= 1 p
Introduction to Spectroscopy (Chapter 6) Electromagnetic radiation (wave) description: Wavelength λ Velocity v Electric Field Strength 0 Amplitude A Time t or Distance x Period p Frequency ν time for 1
CHAPTER 7 SUMMARY OF THE PRESENT WORK AND SUGGESTIONS FOR FUTURE WORK
161 CHAPTER 7 SUMMARY OF THE PRESENT WORK AND SUGGESTIONS FOR FUTURE WORK 7.1 SUMMARY OF THE PRESENT WORK Nonlinear optical materials are required in a wide range of important applications, such as optical
Chapter 6 Photoluminescence Spectroscopy
Chapter 6 Photoluminescence Spectroscopy Course Code: SSCP 4473 Course Name: Spectroscopy & Materials Analysis Sib Krishna Ghoshal (PhD) Advanced Optical Materials Research Group Physics Department, Faculty
ELECTRODYNAMICS OF CONTINUOUS MEDIA
ELECTRODYNAMICS OF CONTINUOUS MEDIA by L. D. LANDAU and E. M. LIFSHITZ Institute of Physical Problems, USSR Academy of Sciences Volume 8 of Course of Theoretical Physics Translated from the Russian by
Semiconductor quantum dots
Semiconductor quantum dots Quantum dots are spherical nanocrystals of semiconducting materials constituted from a few hundreds to a few thousands atoms, characterized by the quantum confinement of the
Multi-Dimensional IR Spectroscopy of Acetic Acid Dimers and Liquid Water
Multi-Dimensional IR Spectroscopy of Acetic Acid Dimers and Liquid Water N. Huse 1, J. Dreyer 1, E.T.J.Nibbering 1, T. Elsaesser 1 B.D. Bruner 2, M.L. Cowan 2, J.R. Dwyer 2, B. Chugh 2, R.J.D. Miller 2
Some applications of photoluminescence for
Some applications of photoluminescence for probing polymer relaxation processes Part 2 p1 Teresa DibZambonAtvars Instituto de Química Universidade Estadual de Campinas Campinas, São Paulo, Brasil E-mail:
1. Photoreduction of Benzophenone in 2-Propanol
1. Photoreduction of Benzophenone in 2-Propanol Topic: photochemistry, photophysics, kinetics, physical-organic chemistry Level: undergraduate physical chemistry Time: 2 x 2 hours (separated by ~24 hours)
The ph-responsive behaviour of aqueous solutions of poly(acrylic acid) is dependent on molar mass
Electronic Supplementary Material (ESI) for Soft Matter. This journal is The Royal Society of Chemistry 2016 The ph-responsive behaviour of aqueous solutions of poly(acrylic acid) is dependent on molar
Lecture 3: Light absorbance
Lecture 3: Light absorbance Perturbation Response 1 Light in Chemistry Light Response 0-3 Absorbance spectrum of benzene 2 Absorption Visible Light in Chemistry S 2 S 1 Fluorescence http://www.microscopyu.com
[Supporting Information]
![[Supporting Information]](/thumbs/95/125112752.jpg "[Supporting Information]")
Transmittance (a.u.) Intensity (a.u.) Intensity (a.u.) [Supporting Information] New methods of synthesis and varied properties of carbon quantum dots with high nitrogen content Sunita Dey, P. Chithaiah,
DEVELOPMENT OF NANO PARTICLE SIZING SYSTEM USING FLUORESCENCE POLARIZATION
XX IMEKO World Congress Metrology for Green Growth September 9 14, 2012, Busan, Republic of Korea DEVELOPMENT OF NANO PARTICLE SIZING SYSTEM USING FLUORESCENCE POLARIZATION Terutake Hayashi, Masaki Michihata,
Time Resoled Pulsed Laser Photolysis Study of Pyrene Fluorescence Quenching by I - Anion
1 Time Resoled Pulsed Laser Photolysis Study of Pyrene Fluorescence Quenching by I - Anion Submitted: February 2, 2014 (CHEM 457, Section 2) Department of Chemistry, The Pennsylvania State University,
Detection of Mercury(II) and Lead(II) with Graphene Oxide- Based Biosensors
Detection of Mercury(II) and Lead(II) with Graphene Oxide- Based Biosensors Ming Li, Nianqiang (Nick) Wu* Mechanical & Aerospace Engineering West Virginia University Morgantown, WV 26506 Presentation Outline
PHOTOCHEMISTRY NOTES - 1 -
- 1 - PHOTOCHEMISTRY NOTES 1 st Law (Grotthus-Draper Law) Only absorbed radiation produces chemical change. Exception inelastic scattering of X- or γ-rays (electronic Raman effect). 2 nd Law (Star-Einstein
ECE280: Nano-Plasmonics and Its Applications. Week8
ECE280: Nano-Plasmonics and Its Applications Week8 Surface Enhanced Raman Scattering (SERS) and Surface Plasmon Amplification by Stimulated Emission of Radiation (SPASER) Raman Scattering Chandrasekhara
MOLECULAR SPECTROSCOPY AND PHOTOCHEMISTRY
20 CHAPTER MOLECULAR SPECTROSCOPY AND PHOTOCHEMISTRY 20.1 Introduction to Molecular Spectroscopy 20.2 Experimental Methods in Molecular Spectroscopy 20.3 Rotational and Vibrational Spectroscopy 20.4 Nuclear
Molecular Luminescence Spectroscopy
Molecular Luminescence Spectroscopy In Molecular Luminescence Spectrometry ( MLS ), molecules of the analyte in solution are excited to give a species whose emission spectrum provides information for qualitative
CHAPTER 3 RESULTS AND DISCUSSION
CHAPTER 3 RESULTS AND DISCUSSION 3.1 CHAPTER OUTLINE This chapter presents the data obtained from the investigation of each of the following possible explanations: (1) Experimental artifacts. (2) Direct
General Considerations 1
General Considerations 1 Absorption or emission of electromagnetic radiation results in a permanent energy transfer from the emitting object or to the absorbing medium. This permanent energy transfer can
Lecture 06. Fundamentals of Lidar Remote Sensing (4) Physical Processes in Lidar
Lecture 06. Fundamentals of Lidar Remote Sensing (4) Physical Processes in Lidar Physical processes in lidar (continued) Doppler effect (Doppler shift and broadening) Boltzmann distribution Reflection
INTRODUCTION TO SCA\ \I\G TUNNELING MICROSCOPY
INTRODUCTION TO SCA\ \I\G TUNNELING MICROSCOPY SECOND EDITION C. JULIAN CHEN Department of Applied Physics and Applied Mathematics, Columbia University, New York OXFORD UNIVERSITY PRESS Contents Preface
Modern Techniques in Applied Molecular Spectroscopy
Modern Techniques in Applied Molecular Spectroscopy Edited by FRANCIS M. MIRABELLA Equistar Chemicals, LP A Wiley-Interscience Publication JOHN WILEY & SONS, INC. New York Chichester Weinheim Brisbane
Electronic Spectra of Complexes
Electronic Spectra of Complexes Interpret electronic spectra of coordination compounds Correlate with bonding Orbital filling and electronic transitions Electron-electron repulsion Application of MO theory
Measuring Colocalization within Fluorescence Microscopy Images
from photonics.com: 03/01/2007 http://www.photonics.com/article.aspx?aid=39341 Measuring Colocalization within Fluorescence Microscopy Images Two-color fluorescence-based methods are uncovering molecular
There are two types of polysaccharides in cell: glycogen and starch Starch and glycogen are polysaccharides that function to store energy Glycogen Glucose obtained from primary sources either remains soluble