Analysis of non-olfactory sensory pathways that are necessary for the olfactory associative learning of the Drosophila mushroom body

Transcription

1 B Analysis of non-olfactory sensory pathways that are necessary for the olfactory associative learning of the Drosophila mushroom body ITO, Kei The mushroom body is known to be an important center for associative olfactory memory in the insect brain. To understand the neural pathways of information other than the olfactory signals toward this center, we first analyzed the organization of the primary gustatory center, whose structure was hardly known despite the importance of gustatory information in associative olfactory learning. We also analyzed the neural connections of the brain regions that receive the arborizations of the input/output neurons of the mushroom body. By systematically labeling neurons according to cell lineage, we found that no neurons from the gustatory, auditory and visual centers directly project to these regions and that there are distinct regions that receive the projections from putative higher-associative centers. H21 6,400,000 1,920,000 8,320,000 H22 5,100,000 1,530,000 6,630,000 H23 3,200, ,000 4,160,000 14,700,000 4,410,000 19,110,000 GAL4 LexA

2 GAL4 GAL4 GAL4 GAL4 GAL4 UAS GFP DsRed GAL4 GFP DsRed GAL4 2 GAL4 GAL4 LexA GAL4 LexA::VP16 LexA lexaop GFP LexA GFP GAL4 LexA GAL4 LexA LexA GAL4

3 3 vertical lobe superior intermediate protocerebrum (SIP) medial lobe crepine (CRE) GAL4 LexA SIP CRE 10 SIP CRE SIP CRE CRE 4 rubus rubus Flybrain Neuron Database 1

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