Morphological Characteristics of the Branchial Nerve in Axolotl Embryos at Different Stages

Transcription

1 Chinese Journal of Zoology 2008,43 (3) : ( , ; ( ) , ) : ( Ambystoma mexicanum), 4 %,,, 30, ; 35, ; 37, ; 38, ; 40,,, ; : ; ; :Q954 :A : (2008) Morphological Characteristics of the Branchial Nerve in Axolotl Embryos at Different Stages HUANG Ming2Yu Takara Shimokawa Rieko Kominami Saturu Yasukata Harumichi Shinohara 3 ( Department of Anatomy, Medical College, Qinghai University, Xining , China ; Division of Anatomy, Kanazawa Medical University, Ishikawa , Japan) Abstract :Axolotl s ( Ambystoma mexicanum) spawn was incubated at 25, and embryos at stages were collected and fixed with 4 % paraformaldehyde. The whole mount immunostaining was conducted and embryos were observed under stereomicroscope. The sensory ganglion of branchial nerve and smallish branchial nerve trunk were observed in the stage 30 embryo, rami and communicating ramus of branchial nerve appeared in the stage 35 embryo, maxillary and mandibular nerves developed in the stage 37 embryo, dorsal ramus and pharynges ramus of glossopharyngeal nerve were found in the stage 38 embryo, and pretrematicus ramus of glossopharyngeal nerve were observed in the stage 40 embryo. Early stage axolotl embryo kept the same pattern of branchial nerves as in the ancestral vertebrates. In later stage, embryo showed the evolution tendency of the branchial apparatus and branchial nerves appearance and redistribution. characteristic of branchial nerve. Key words :Axolotl ; Branchial nerve ; Glossopharyngeal nerve The glossopharyngeal nerve of axolotl kept the original morphological, ( ), 2003 ; 3,E2mail ac. jp ;,,; : ; E2mail com : , :

2 122 Chinese Journal of Zoology 43,,,,, ( ) [1 ] ;,, ( Ambystoma mexicanum), [1 ], ( ) Bordzilovskaya Detlaff [2 ], , 4 % 112 [3 ], PBS, ; TBST ( 150 mmol NaCl 100 mmol Tris2HCl (ph 715) 011 % Tween) 10 min 2, 115 % H 2 O 2 2 TBST (30 % H 2 O 2 45 l + TBST 955 l) 60 min, ; TBST, 115 % Blocking (Blocking Reagent 115 g + TBST 100 ml) 60 min (,, ) ; (3A10,Developmental Studies Hybridoma Bank),4,36 h, ; TBST, ; 115 % Blocking 90 min, IgG, 4, 16 h ; TBST, DBA2H 2 O 2 ( TBST 1 ml + DBA 015 g + 30 % H 2 O l),,, BABB (1 1 ), 2 30, 102 h 415 mm,,,,,,, 2 ; ;, 31, 109 h 417 mm,,,, ; ;, 2,,, 35, 122 h 6125 mm,,,,,; ( :A), 37, 177 h 715 mm

3 3 : 123,,,, ; ; ;, ; ( : B) 38, 178 h, 718 mm,, 2 40, 240 h 913 mm,,,, ( :C) 3,, ( ),, [4 ],,,, ( 1 ) ( 2 3 ) ( ) ( ) ( ),, 2, ;,,,,,, Northcutt [5 ] 37, ; [2 ], 39,;, 37 30, ( ), ; 35,, Northcutt [5 ], 26 30, 35, 30,,31,, 35,, [4 ],, ; ;,,,

4 124 Chinese Journal of Zoology 43, , Tanaka [1 ], 2 [1 ],,, [6 ],,, 40,,, [7 ],,,,,,,, [1 ] Tanaka S, Mizukami S. Microscopical study of the nervus glossopharyngeus of the urodeles Triturus pyrrhogaster ensicauda, with reference to communications of this nerve with the nervus facialis. Zool Jb Anat, 1992, 122(3) : [2 ] Bordzilovskaya N P, Detlaff T A. Table of stages of the normal development of axolotl embryos and the prognostication of timing of successive developmental stages at various temperatures. Axolotl Newsletter, 1979, 7 (1) : [3 ]. in situ g g g g g g } g g g. :,2002, [4 ] Romer A S, Parsons T S. The Vertebrate Body ( Sixth Edition). Tokyo : Saunders College Publishing, Cas Publishing Japan Ltd, 1986, [5 ] Northcutt R G, Brgndle K. Development of branchiomeric and lateral line nerves in the axolotl. Neurology, 2004, 355 (3) : The Journal of Comparative [6 ] Kuratani S, Tanaka S, Ishikawa Y, et al. Early development of the facial nerve in the chick embryo with special reference to the development of the chorda tympani. of Anatomy, 1988, 182(2) : The American Journal [7 ] Kuratani S, Ueki T, Aizawa S, et al. Peripheral development of cranial nerves in a cyclostome, lampetra japonica : morphological distribution of nerve branches and the vertebrate body plan. The Journal of Comparative Neurology, 1997, 384 (2) : A :35 ; B :37 ; C:40 ( 10) 1. 2 ; 2. ; 3. ; 4. 2 ; 5. ; 6. ; 7. ; 8. ; 1. ; 2. ; 3. ; ;. ; ;. Ot. ; EB. Explanation of Plate A : Left lateral view of external branchial region of stage 35 Axolotl embryo ; B : Left lateral view of external branchial region of stage 37 Axolotl embryo ; C: Left lateral view of external branchial region of stage 40 Axolotl embryo. ( 10) 1. 2 communicating ramus ; 2. Gill ramus of glossopharyngeal nerve ; 3. Gill ramus of vagus nerve ; 4. Communicating ramus of 2 gill ramus ; 5. Branchial arch artery ; 6. External gills ; 7. Pretrematicus ramus of glossopharyngeal nerve ; 8. Posttrematicus ramus of glossopharyngeal nerve ; 1. Ophthalmic profundus nerve ; 2. Maxillary nerve ; 3. Mandibular nerve ; The root of maxillary nerve and mandibular nerve ;. Cranial nerve ;. Glossopharyngeal nerve ;. Vagus nerve ; Ot. Otocyst ; EB. Eyeball.

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