Relationship bet ween active components in Scutell a ria baica lensis and environmental factors in mounta inous region of Western Beijing

Transcription

1 Chinese Traditional and Herbal Drugs , 3,,,, (,,, ) :, m 8 85, HPLC A ;,, ;,, ;, 26,, 3 4,, : ; ; ; ; :R :A : (2009) Relationship bet ween active components in Scutell a ria baica lensis and environmental factors in mounta inous region of Western Beijing XIAO Yan2qing, J IAN G Yuan, L I Lei, ZHAN G Wen2tao, L I Yu2ping, ZHAN G Wen2sheng ( Key Laboratory of Traditional Chinese Medicine Resource Protection and Utilization, Beijing Municipality State Key Laboratory of Earth Surface Processes and Resource Ecology College of Resources Science and Technology, Beijing Normal University, Beijing , China) Abstract : Objective To reveal t he influence of environmental factors on t he active component s in S cutell ari a baicalensis t hrough analyzing t he data sampled f rom t he herbal plant s along t he altit udinal gradient in mountainous region of Western Beijing. Methods In total 85 individuals of S. baicalensis herbs were collected and t he surface soils under each of t he plant were also sampled f rom eight different habitat s, wit h an interval of about m vertically ranging f rom 800 to m in August t hrough September, The content s of active component s in the herbal samples, namely baicalin, wogonoside, baicalein, wogonin, and oroxylin A were determined by HPL C. The organic matter and t he total nitrogen co ntent s in t he soil samples were analyzed t hro ugh standard measurement p rocess. The vario us habitat conditions represented by temperat ure were estimated t hrough a vertical lap se rate of / 100 m, and t hose by precipitation and solar radiation calculated according to t he isoline map s. Results The signi2 ficant positive correlations were observed only between the content s of baicalin, wogonoside and the eleva2 tion. Bot h t hose two content s showed the significant negative correlations wit h t he annual mean tempera2 ture and precipitation, while showed positive wit h annual mean solar radiation. No coorelation wit h any statistical significance was observed between t he content s of active component s in S. baicalensis and the soil parameters in t his st udy. Conclusion It seems t hat t he plant s of S. baicalensis growing in a habitat wit h annual mean temperat ure wit hin a range of 2 6 could concentrate relatively rich content s of active co mpo nent s. Wit hin t he nat ural dist ributio n areas of S. baicalensis, t he habitat s wit h an annual tem2 perat ure averaged at 3 4 tend to be t he most fitting sites for growing best quality S. baicalensis rich in active component s, and t ho se sites are almost coincident wit h t he environment for trueborn production area of t raditio nal S. baicalensis herbs. 3 : : (2006BAI09B0225) ; ( ) :(1982 ),,,, Tel : (010) E2mail : cn 3 E2mail : cn

2 1292 Chinese Traditional and Herbal Drugs Key words : S cutell ari a baicalensis Georgi ; content s of active component s ; environmental factors ; fitting habitat s ; mountainous region of Western Beijing S cutellari a baicalensis Georgi,,,,, [1 ], 47 [2 ], A,, ( ) [ 38 ],,,,,,,,, [9 ], [10 ], [11 ],, m [12 ],,,,,,,, , m 8 85,HPL C 5,, :,, , , m , , 2 8, mm, Mega J / m 2 (1) 1 Table 1 Climate conditions of plots for sampling / E S. baicalensis herbs at different elevations in mountainous region of Western Beijing / W / m / / mm / (M J m 22 ) :, , 1118, 9118 m,100 m, 0156 [13 ], 113 :2007 8,( m), 100 m 10, m ,, m 3

3 Chinese Traditional and Herbal Drugs ,, 10 cm 2 2 / m Table 2 Habitat conditions of plots for sampling S. baicalensis herbs at different elevations in mountainous region of Western Beijing / / % > < / / : Waters Waters , ; A ( 98 %), : 212 : Symmetry Shield TM RP18 ( mm, 5 m) ;: 2011 % ( ),020 min,32 % 45 %,2025 min,45 % 50 %,2532 min,50 % ; : 275 nm ; :110 ml/ min ; :27 ;:10L 213 :,, [14 ], [6,15 ],, 01 cm ( ) 12 cm ( ),,,,, 65, 214 : 011 g,,70 % 50 ml, 40 min,, 0145m, 215 :30 mg, A 5 mg, 10 ml,70 %,, A 70 % 5 ml 115 ml 1 ml A 014 ml,70 %, 216 : , 10L,, ( X),( Y), : Y = X , r 2 = ( mg/ ml ) ; : Y = X , r 2 = ( mg/ ml) ;: Y = X , r 2 = ( mg/ ml) ; : Y = X , r 2 = ( mg/ ml ) ; A : Y = X , r 2 = ( mg/ ml) 217 :, 10 L, 6,5 RSD 2 % 218 : 6, 011 g, 214,, 5 RSD 3 % 219 : h,5 RSD 111 %, 12 h 2110 : 6, 0105 g,, 214, 5 95 %104 % 3, 2, EXCEL SPSS :SPSS

4 1294 Chinese Traditional and Herbal Drugs ,, 0101,,, A 0105,3 (3) 3 5 Table 3 Correlation between elevations and contents of f ive active components in S. baicalensis r 2 = A, 971 m, A m, m (3) / % A / m pearson ( ) Correlation is significant at level,5,,,971 m,,, m,,1 590 m,,,,,1 590 m (1 2) 1 Fig. 1 Average contents of baicalin in S. baicalensis from different elevation habitats SPSS,, : Y = X , r 2 = ; : Y = X ,, m,, ;971 m, A m, :,,

5 Chinese Traditional and Herbal Drugs ,, (4) 4 5 Table 4 Correlations between environment factors and con2 tents of f ive active components in S. baicalensis in mountainous region of Western Beijing / % A / Pearson ( ) / mm Pearson ( ) / Pearson (M J m 22 ) ( ) / % Pearson ( ) / % Pearson ( ) Correlation is significant at level 3 Correlation is significant at level,,,,,, :,, mm, ; M J / m 2, 28,,,, 26,34,, 6, 3, 5 511,5,,, ; A,, 512,,, ,, 34,,,, 514,,,,,, 515,,, : [1 ] [ S] [2 ], 1 [J ]1, 1994, 9 (4) : [3 ],,, 1

6 1296 Chinese Traditional and Herbal Drugs [J ]1, 2008, 39 (4) : [ 4 ],,, 1 [J ]1, 2005, 30 (7) : [ 5 ], 1 [J ]1, 2002, 25 (2) : [ 6 ],,, 1 RP2HPLC [J ]1, 2007, 4 (2) : [ 7 ], 1 HPLC [J ]1, 2001, 32 (3) : [ 8 ],,, 1 [J ]1, 2003 (3) : [ 9 ],,, 1 [J ]1, 1999, 25 (2) : [ 10 ],,, 1 [J ]1, 2005, 21 (3) : [ 11 ],,, 1 [J ]1, 2006, 41 (2) : [ 12 ] 1 [ M ]1 :, 1977, 65 (2) : 1941 [ 13 ],,, 1 [J ]1, 2006, 24 (4) : [ 14 ],, 1 [J ]1, 2001 (3) : [ 15 ],,, 1 () [ M ]1 :, AFLP 2, 2, 1,2, 1 3, 1, 2 3 (11, ; 21, ) :9 A FL P, (A FL P),DNA,32,,N TSYS2PC, SDS DNA, A FL P ;32,10 ; AFL P,9 ;,9 2, :; ; AFL P ; :R :A : (2009) AFLP Analysis of genetic diversity of Leonurus j a ponicus germplasm resources YU Qi 2, SH EN Xiao2xia 2, SH EN Yu2feng 1,2, CH EN Jia2hong 1, SH I Cong2guang 1, WAN G Zhi2an 2 (11 College of Agriculture and Biotechnology, Zhejiang University, Hangzhou , China ; 2. Zhejiang Research Institute of Chinese Materia Medica, Hangzhou , China) Abstract : Objective The A FL P analysis of nine accessions of L eonurus j a ponicus was conducted to explore genetic diversities among germplasm. Methods Genetic diversities at DNA level among t he germ2 plasm of L. j a ponicus were studied by t he amplified f ragment length polymorp hism (A FL P) marker, and 32 pair s of selective amplificatio n p rimer were identified. The similarities amo ng t he germplasm of L. j a ponicus were calculated and analyzed by t he sof t ware of N TS YS2PC, and genetic evolutio n t ree was developed, using amplified f ragment s as p rimary matrix. Results The quality of genomic DNA of L. j a ponicus ext racted by SDS met ho d was good eno ugh to meet t he requirement of A FL P analysis. Ten combinations wit h multiple morp hologies, clear bands, and high discrimination rate were identified f rom 32 pairs of selective amplification primers. The A FL P fingerprinting was developed, which could differen2 tiate each of nine accessions of L. j a ponicus. Nine accessions of L. j a ponicus could be classified into two types based on t he evolution tree. Conclusion There are richer genetic diversities among t he germplasm resources of L. j a ponicus, and certain relationship s are found between some of morp hological characteris2 tics and genot ypes. 3 : : (2005C ) : (1965 ),,,,, Tel : (0571) E2mail : mail. com 3 Tel : (0571) E2mail : cn