Intravital Imaging Reveals Ghost Fibers as Architectural Units Guiding Myogenic Progenitors during Regeneration

Size: px
Start display at page:

Download "Intravital Imaging Reveals Ghost Fibers as Architectural Units Guiding Myogenic Progenitors during Regeneration"

Transcription

1 Cell Stem Cell Supplemental Information Intravital Imaging Reveals Ghost Fibers as Architectural Units Guiding Myogenic Progenitors during Regeneration Micah T. Webster, Uri Manor, Jennifer Lippincott-Schwartz, and Chen-Ming Fan

2 Supplemental Information

3 Figure S1, Related to Figure 1. Characterizing the SC lineage during regeneration by IVM (A and B) Tamoxfien (TMX) induced labeling of Pax7+ SCs in uninjured (A) Pax7 cre/ert2 ; R26R eyfp/eyfp and (B) Pax7 cre/ert2 ; R26R GR/+ TA muscle cross sections probed with anti-pax7 and anti-gfp; top panels with TMX, bottom panels without TMX control; arrows, colocalized signal. (C) Quantification for (A and B); percentage of Pax7+ cells labeled with either eyfp or H2B-GFP; 100 cells for each of 3 samples. (D) IVM setup is shown; mouse is on microscope stage secured to anesthesia source by green tape, exposed TA is facing inverted objective (not seen). Inset, modified Fluorodish with portions of wall removed for mounting onto mouse. (E) IVM of SHG from sarcomeres, ~4 per 10 µm, similar to that in gastrocnemius myofibers (Llewellyn et al., 2008); plane of perspective, bottom left. (F) TA myofiber and ghost fiber diameters (µm) measured in uninjured (sarcomere SHG) and CTX-injured (collagen SHG) muscle, respectively; 100 fibers measured; p =.15, t-test. (G) Longitudinal section of 2 dpi TA imaged by SHG from collagen (SHG) then post probed for ECM components of the basal lamina, Collagen I (Col I) and Laminin (Lam); arrows, examples of overlap between SHG signal, Collagen I, and Laminin. (H) eyfp+ MPs (green) at 2 dpi; arrows, ghost fiber collagen SHG, red. Collagen SHG also present crossing surface of ghost fibers (Gillies and Lieber, 2011) in the y-axis. (I) Number of eyfp+ (uninjured to 3 dpi) or H2B-GFP+ (4 dpi) cells per 620 µm length of individual ghost fibers; n = 50 ghost fibers per day. Error bars = s.e.m; scale bars = 10 µm in (A, B, E), 50 µm (H), 100 µm in (G).

4

5 Figure S2, Related to Figure 2. MP divisions occur on the ghost fiber interior surface (A) Division frequencies of SC (uninjured, 0 dpi) and MPs at indicated dpi. Frequency represented as number of divisions per total labeled cells in the IVM field per 1 h period. Color code denotes the hour of the IVM session during which the observed divisions occurred; 120 cells per day. (B) Division frequencies of MPs in individual ghost fibers at indicated dpi. Frequency represented as number of divisions per total labeled cells per 620 µm ghost fiber length per 1 h period; 110 cells per day. (C) Radar plots representing percentage (wedge length) of H2B-GFP+ cell division angles (15 bins) relative to the x- and y-axes (top row) and x- and z-axes (bottom row) at given dpi; 24 cells per day; dashed line = 45. (D) Radar plots representing percentage of eyfp+ cell division angles (15 bins) relative to the x- and y-axes (left) and x- and z-axes (right); 20 cells; dashed line = 45. (E) IVM image of H2B-GFP+ cells (white) localized within ghost fibers (red; arrows) at 3 dpi. Anaphase chromosomes (arrowheads) are parallel to x-axis. Faint collagen SHG also present crossing surface of ghost fibers (Gillies and Lieber, 2011) in the y-axis. (F) Pax7 cre/ert2 ; R26R eyfp/eyfp 3 dpi TA longitudinal section probed with anti-eyfp and anti-laminin and counterstained for DAPI. eyfp+ anaphase cell (arrows) is on the interior of the ghost fiber and dividing parallel to Laminin along the longitudinal, x-axis. Note: ghost fiber surface supporting the cell is below the imaged optical plane. Scale bars = 50 µm.

6

7 Figure S3, Related to Figure 3. MPs migrate bi-directionally along the longitudinal, x-axis of the ghost fiber (A) Individual MP migratory track displacements (µm) in the y-axis at given dpi; 14 cells for each day; red dash = mean. (B) Same as (A) but measured in the z-axis at given dpi. (C) Percentage of eyfp+ MPs localized to the inside of ghost fibers (defined by anti-laminin) or to the interstitial space; 320 cells; error bars = s.e.m. (D) Pax7 cre/ert2 ; R26R eyfp/eyfp 3-dpi TA longitudinal section probed with anti-eyfp and anti-laminin and counterstained with DAPI. eyfp+ cells (arrows) localize within ghost fiber. Newly divided daughter cells (arrow heads) in contact with ghost fiber. (E to G) 3 dpi IVM time-points of dividing eyfp+ MPs (*). (E) Daughters migrate away from each other in the same direction that they divided. (F) Daughters migrate away from each other in the opposite direction that they divided. (G) Daughter on the right follows daughter on the left and two cells migrate together. Minutes elapsed, upper right for (E to G). (H) Pie chart shows distribution of MP daughters that separate following the direction established by division (blue, example in (E)), that separate opposite the direction established by division (orange, example in (F)), or that follow to the right (green) or left (yellow, as in (G)); 52 divisions. (I) Diagram of single needle track (NT) injury. The needle-puncture (black circle) triggers degeneration of muscle fibers (yellow) causing the injured region to extend distally, D, from the injury-site, I, down the x-axis. Ends of degenerated fibers next to segments of uninjured myofibers denoted as junction sites, J. (J and K) 3 d post NT IVM images of MPs (green) and SHG from sarcomeres and collagen (red). (J) 3D projection of region D (as in (I)). MPs align with and are evenly spaced along ghost fibers. Collagen fibers crisscross the undamaged, perimysial layer at the surface (top). (K) Maximal projection of (J) from the perspective of the x-axis. (L) 3 d post NT injury IVM time-points of eyfp+ MPs at junction site (J, defined as junction between sarcomere SHG (white dashed line) and absence of SHG signal). MPs around the junction display limited mobility from 0 min (green) to 60 min (red). Scale bars = 100 µm in (D, J, K, L), 50 µm in (E to G).

8

9 Figure S4, Related to Figure 4. Ghost fibers govern MP migration, division, and organization of regenerated muscle (A) Quartile distribution of MP straightness 3 d after rotation either in rotated ghost fibers or in interstitial space; 44 cells, p<.0001 (*), t-test. (B and C) Additional examples of Pax7 cre/ert2 ; R26R eyfp/eyfp longitudinal sections from 14 d post rotation muscle. (B) Probed with anti-eyfp and anti-mhc and counterstained with DAPI. Newly regenerated fibers (eyfp+ MHC+) in the rotated region (white dashed line) skew towards the y-axis. Uninjured fibers (eyfp- MHC+) at bottom of image aligned towards with x-axis. (C) Probed with anti-eyfp, anti-myosin heavy chain (MHC), and anti-laminin, and counterstained with DAPI. Arrow points to branched fiber where rotated muscle (directed up towards y-axis) comes into line with injured, non-rotated muscle in the x-axis. Scale bars = 100 µm in (B), and 50 µm in (C).

10 Movie S1, related to Figure 2. H2B-GFP+ MP divides along the ghost fiber longitudinal axis Three dpi time-lapse IVM of H2B-GFP+ MP (green) dividing in ghost fibers (red, collagen SHG). Movie frames are Z stack maximal projections comprised of 12 optical sections spaced 3 µm apart and captured at 3 min intervals for 1 h and 6 min. Playback, 10 fps; x-axis right to left, y-axis up and down; scale bar = 25 µm. Movie S2, related to Figure 3. 3D collective eyfp+ MP migration resembles a tube Three dpi 3D time-lapse IVM of eyfp+ MPs (green). Movie frames are comprised of 31 optical sections spaced 3 µm apart and captured at 2 min intervals for 58 min. Playback, 10 fps; x-axis right to left, y-axis up and down, z-axis into image plane; scale bar = 50 µm. Movie S3, related to Figure 3. 3D collective eyfp+ MP migration resembles a tube Three dpi 3D time-lapse IVM of eyfp+ MPs (green), cropped from of Movie S2. Movie frames are comprised of 31 optical sections spaced 3 µm apart and captured at 2 min intervals for 58 min. Playback, 10 fps; x-axis into image plane, y-axis left to right, z-axis up and down; scale bar = 50 µm. Movie S4, related to Figure 3. eyfp+ MP bi-directional streaming and division Three dpi time-lapse IVM of eyfp+ MPs. Movie frames are Z stack maximal projections comprised of 50 optical sections spaced 3 µm apart and captured at 2 min intervals for 5 h and 12 min. Playback, 10 fps; x-axis right to left, y-axis up and down; scale bar = 100 µm. Supplemental References Gillies, A. R. & Lieber, R. L. (2011). Structure and function of the skeletal muscle extracellular matrix. Muscle Nerve 44, Llewellyn, M.E., Barretto, R.P.J., Delp, S.L., and Schnitzer, M.J. (2008). Minimally invasive high-speed imaging of sarcomere contractile dynamics in mice and humans. Nature 454,

Mitochondrial Dynamics Is a Distinguishing Feature of Skeletal Muscle Fiber Types and Regulates Organellar Compartmentalization

Mitochondrial Dynamics Is a Distinguishing Feature of Skeletal Muscle Fiber Types and Regulates Organellar Compartmentalization Cell Metabolism Supplemental Information Mitochondrial Dynamics Is a Distinguishing Feature of Skeletal Muscle Fiber Types and Regulates Organellar Compartmentalization Prashant Mishra, Grigor Varuzhanyan,

More information

T H E J O U R N A L O F C E L L B I O L O G Y

T H E J O U R N A L O F C E L L B I O L O G Y T H E J O U R N A L O F C E L L B I O L O G Y Supplemental material Eisner et al., http://www.jcb.org/cgi/content/full/jcb.201312066/dc1 Figure S1. Mitochondrial continuity in adult skeletal muscle. (A)

More information

In ovo time-lapse analysis after dorsal neural tube ablation shows rerouting of chick hindbrain neural crest

In ovo time-lapse analysis after dorsal neural tube ablation shows rerouting of chick hindbrain neural crest In ovo time-lapse analysis after dorsal neural tube ablation shows rerouting of chick hindbrain neural crest Paul Kulesa, Marianne Bronner-Fraser and Scott Fraser (2000) Presented by Diandra Lucia Background

More information

LIST of SUPPLEMENTARY MATERIALS

LIST of SUPPLEMENTARY MATERIALS LIST of SUPPLEMENTARY MATERIALS Mir et al., Dense Bicoid Hubs Accentuate Binding along the Morphogen Gradient Supplemental Movie S1 (Related to Figure 1). Movies corresponding to the still frames shown

More information

DOI: 10.1038/ncb2819 Gαi3 / Actin / Acetylated Tubulin Gαi3 / Actin / Acetylated Tubulin a a Gαi3 a Actin Gαi3 WT Gαi3 WT Gαi3 WT b b Gαi3 b Actin Gαi3 KO Gαi3 KO Gαi3 KO # # Figure S1 Loss of protein

More information

Collisions and conservation laws

Collisions and conservation laws (ta initials) first name (print) last name (print) brock id (ab17cd) (lab date) Experiment 4 Collisions and conservation laws Prelab preparation Print a copy of this experiment to bring to your scheduled

More information

Supplemental Information. Inferring Cell-State Transition. Dynamics from Lineage Trees. and Endpoint Single-Cell Measurements

Supplemental Information. Inferring Cell-State Transition. Dynamics from Lineage Trees. and Endpoint Single-Cell Measurements Cell Systems, Volume 3 Supplemental Information Inferring Cell-State Transition Dynamics from Lineage Trees and Endpoint Single-Cell Measurements Sahand Hormoz, Zakary S. Singer, James M. Linton, Yaron

More information

Supplementary Information for. Single-cell dynamics of the chromosome replication and cell division cycles in mycobacteria

Supplementary Information for. Single-cell dynamics of the chromosome replication and cell division cycles in mycobacteria Supplementary Information for Single-cell dynamics of the chromosome replication and cell division cycles in mycobacteria Isabella Santi 1 *, Neeraj Dhar 1, Djenet Bousbaine 1, Yuichi Wakamoto, John D.

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION doi:10.1038/nature09414 Supplementary Figure 1 FACS-isolated 8c hepatocytes are highly pure. a, Gating strategy for identifying hepatocyte populations based on DNA content. b, Detection of mchry and mchr9

More information

Nature Protocols: doi: /nprot Supplementary Figure 1

Nature Protocols: doi: /nprot Supplementary Figure 1 Supplementary Figure 1 Photographs of the 3D-MTC device and the confocal fluorescence microscopy. I: The system consists of a Leica SP8-Confocal microscope (with an option of STED), a confocal PC, a 3D-MTC

More information

Electric Fields and Equipotentials

Electric Fields and Equipotentials Electric Fields and Equipotentials Note: There is a lot to do in this lab. If you waste time doing the first parts, you will not have time to do later ones. Please read this handout before you come to

More information

SUPPLEMENTARY FIGURES AND TABLES AND THEIR LEGENDS. Transient infection of the zebrafish notochord triggers chronic inflammation

SUPPLEMENTARY FIGURES AND TABLES AND THEIR LEGENDS. Transient infection of the zebrafish notochord triggers chronic inflammation SUPPLEMENTARY FIGURES AND TABLES AND THEIR LEGENDS Transient infection of the zebrafish notochord triggers chronic inflammation Mai Nguyen-Chi 1,2,5, Quang Tien Phan 1,2,5, Catherine Gonzalez 1,2, Jean-François

More information

Nature Biotechnology: doi: /nbt Supplementary Figure 1. Overexpression of YFP::GPR-1 in the germline.

Nature Biotechnology: doi: /nbt Supplementary Figure 1. Overexpression of YFP::GPR-1 in the germline. Supplementary Figure 1 Overexpression of YFP::GPR-1 in the germline. The pie-1 promoter and 3 utr were used to express yfp::gpr-1 in the germline. Expression levels from the yfp::gpr-1(cai 1.0)-expressing

More information

Supplementary Materials for

Supplementary Materials for www.sciencesignaling.org/cgi/content/full/6/301/ra98/dc1 Supplementary Materials for Regulation of Epithelial Morphogenesis by the G Protein Coupled Receptor Mist and Its Ligand Fog Alyssa J. Manning,

More information

Figure S1. Programmed cell death in the AB lineage occurs in temporally distinct

Figure S1. Programmed cell death in the AB lineage occurs in temporally distinct SUPPLEMENTAL FIGURE LEGENDS Figure S1. Programmed cell death in the AB lineage occurs in temporally distinct waves. (A) A representative sub-lineage (ABala) of the C. elegans lineage tree (adapted from

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION SUPPLEMENTARY INFORMATION doi:10.1038/nature12791 Supplementary Figure 1 (1/3) WWW.NATURE.COM/NATURE 1 RESEARCH SUPPLEMENTARY INFORMATION Supplementary Figure 1 (2/3) 2 WWW.NATURE.COM/NATURE SUPPLEMENTARY

More information

Supplementary Figure 1: Micromechanical cleavage of graphene on oxygen plasma treated Si/SiO2. Supplementary Figure 2: Comparison of hbn yield.

Supplementary Figure 1: Micromechanical cleavage of graphene on oxygen plasma treated Si/SiO2. Supplementary Figure 2: Comparison of hbn yield. 1 2 3 4 Supplementary Figure 1: Micromechanical cleavage of graphene on oxygen plasma treated Si/SiO 2. Optical microscopy images of three examples of large single layer graphene flakes cleaved on a single

More information

Fig. 1. Longitudinal section of the girdle of a 3-year-old lateral branch in Fuyu 3 weeks after

Fig. 1. Longitudinal section of the girdle of a 3-year-old lateral branch in Fuyu 3 weeks after Fig. 1. Longitudinal section of the girdle of a 3-year-old lateral branch in Fuyu 3 weeks after girdling. The left and right sides are the upper and lower cuts of the wound, respectively. The callus pads

More information

Figure 1. Automated 4D detection and tracking of endosomes and polarity markers.

Figure 1. Automated 4D detection and tracking of endosomes and polarity markers. A t = 0 z t = 90 B t = 0 z t = 90 C t = 0-90 t = 0-180 t = 0-300 Figure 1. Automated 4D detection and tracking of endosomes and polarity markers. A: Three different z planes of a dividing SOP shown at

More information

Supplementary Figure 1

Supplementary Figure 1 Supplementary Figure 1 Single-cell RNA sequencing reveals unique sets of neuropeptides, transcription factors and receptors in specific types of sympathetic neurons (a) Dissection of paravertebral SGs

More information

Lecture 13, 05 October 2004 Chapter 10, Muscle. Vertebrate Physiology ECOL 437 University of Arizona Fall instr: Kevin Bonine t.a.

Lecture 13, 05 October 2004 Chapter 10, Muscle. Vertebrate Physiology ECOL 437 University of Arizona Fall instr: Kevin Bonine t.a. Lecture 13, 05 October 2004 Chapter 10, Muscle Vertebrate Physiology ECOL 437 University of Arizona Fall 2004 instr: Kevin Bonine t.a.: Nate Swenson Vertebrate Physiology 437 18 1. Muscle A. Sarcomere

More information

Assignment 7 Due February 26

Assignment 7 Due February 26 Assignment 7 Due February 26 Cells of Multicellular organisms 1. File upload (3 points) View this electron micrograph of spinach leaf cells. The central cell has a thin cell wall; it is difficult to distinguish

More information

Muscle tissue. Types. Functions. Cardiac, Smooth, and Skeletal

Muscle tissue. Types. Functions. Cardiac, Smooth, and Skeletal Types Cardiac, Smooth, and Skeletal Functions movements posture and body position Support soft tissues Guard openings body temperature nutrient reserves Muscle tissue Special Characteristics of Muscle

More information

Supplementary Figures

Supplementary Figures Supplementary Figures Supplementary Figure 1. Sarcomere length-dependence of total fluorescence intensity in a relaxed muscle fibre containing BSR-RLC. a) Fluorescence intensity (I) relative to the value

More information

Meiosis. Bởi: OpenStaxCollege

Meiosis. Bởi: OpenStaxCollege Meiosis Bởi: OpenStaxCollege Sexual reproduction requires fertilization, a union of two cells from two individual organisms. If those two cells each contain one set of chromosomes, then the resulting cell

More information

Fig. 1. Stereo images showing (A) the best fit of the atomic model for F actin and the F actin map obtained by cryo-em and image analysis, and (B) goo

Fig. 1. Stereo images showing (A) the best fit of the atomic model for F actin and the F actin map obtained by cryo-em and image analysis, and (B) goo Fig. 1. Stereo images showing (A) the best fit of the atomic model for F actin and the F actin map obtained by cryo-em and image analysis, and (B) good correspondence between the location of Cys374 and

More information

Supporting Information

Supporting Information Supporting Information Visualizing the Effect of Partial Oxide Formation on Single Silver Nanoparticle Electrodissolution Vignesh Sundaresan, Joseph W. Monaghan, and Katherine A. Willets* Department of

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION doi:10.1038/nature09450 Supplementary Table 1 Summary of kinetic parameters. Kinetic parameters were V = V / 1 K / ATP and obtained using the relationships max ( + m [ ]) V d s /( 1/ k [ ATP] + 1 k ) =,

More information

AP Biology. Biology is the only subject in which multiplication is the same thing as division. The Cell Cycle: Cell Growth, Cell Division

AP Biology. Biology is the only subject in which multiplication is the same thing as division. The Cell Cycle: Cell Growth, Cell Division QuickTime and and a TIFF TIFF (Uncompressed) decompressor are are needed needed to to see see this this picture. picture. Biology is the only subject in which multiplication is the same thing as division

More information

T H E J O U R N A L O F C E L L B I O L O G Y

T H E J O U R N A L O F C E L L B I O L O G Y Supplemental material Kasprowicz et al., http://www.jcb.org/cgi/content/full/jcb.201310090/dc1 T H E J O U R N A L O F C E L L B I O L O G Y Figure S1. NMJ morphology of shi 12-12B ; shi-4c not treated

More information

According to the diagram, which of the following is NOT true?

According to the diagram, which of the following is NOT true? Instructions: Review Chapter 44 on muscular-skeletal systems and locomotion, and then complete the following Blackboard activity. This activity will introduce topics that will be covered in the next few

More information

Supplementary Figure 1 Interlayer exciton PL peak position and heterostructure twisting angle. a, Photoluminescence from the interlayer exciton for

Supplementary Figure 1 Interlayer exciton PL peak position and heterostructure twisting angle. a, Photoluminescence from the interlayer exciton for Supplementary Figure 1 Interlayer exciton PL peak position and heterostructure twisting angle. a, Photoluminescence from the interlayer exciton for six WSe 2 -MoSe 2 heterostructures under cw laser excitation

More information

Fundamentals of Neurosciences. Smooth Muscle. Dr. Kumar Sambamurti 613-SEI; ;

Fundamentals of Neurosciences. Smooth Muscle. Dr. Kumar Sambamurti 613-SEI; ; Fundamentals of Neurosciences Smooth Muscle Dr. Kumar Sambamurti 613-SEI; 792-4315; sambak@musc.edu 1 Smooth Muscle Structure Cells much smaller than skeletal muscle (2-5µM diam, 100-400µM long) Single

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION doi:10.1038/nature11492 Figure S1 Short-period Seismic Energy Release Pattern Imaged by F-net. (a) Locations of broadband seismograph stations in Japanese F-net used for the 0.5-2.0 Hz P wave back-projection

More information

Supplementary Materials for

Supplementary Materials for advances.sciencemag.org/cgi/content/full/2/8/e1600060/dc1 Supplementary Materials for Analysis of neural crest derived clones reveals novel aspects of facial development Marketa Kaucka, Evgeny Ivashkin,

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION DOI: 10.1038/ncb2215 Figure S1 Number of egfp-vps4a bursts versus cellular expression levels. The total number of egfp-vps4a bursts, counted at the end of each movie (frame 2000, after 1h 28 min) are plotted

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION GP2 Type I-piliated bacteria FAE M cell M cell pocket idc T cell mdc Generation of antigenspecific T cells Induction of antigen-specific mucosal immune response Supplementary Figure 1 Schematic diagram

More information

Experimental test of an expression for the decay of an autocorrelation function

Experimental test of an expression for the decay of an autocorrelation function Supplemental Material for: Experimental test of an expression for the decay of an autocorrelation function Journal: Physical Review Letters Authors: Zach Haralson and J. Goree I. Experimental setup The

More information

Role of Mitochondrial Remodeling in Programmed Cell Death in

Role of Mitochondrial Remodeling in Programmed Cell Death in Developmental Cell, Vol. 12 Supplementary Data Role of Mitochondrial Remodeling in Programmed Cell Death in Drosophila melanogaster Gaurav Goyal, Brennan Fell, Apurva Sarin, Richard J. Youle, V. Sriram.

More information

Supplementary Figure 1. Phenotype of the HI strain.

Supplementary Figure 1. Phenotype of the HI strain. Supplementary Figure 1. Phenotype of the HI strain. (A) Phenotype of the HI and wild type plant after flowering (~1month). Wild type plant is tall with well elongated inflorescence. All four HI plants

More information

Equipotentials and Electric Fields

Equipotentials and Electric Fields Equipotentials and Electric Fields PURPOSE In this lab, we will investigate the relationship between the equipotential surfaces and electric field lines in the region around several different electrode

More information

Modelling Muscle Contraction a multiscale approach

Modelling Muscle Contraction a multiscale approach Porto Ercole, M&MKT 2016 Multiscale Systems from Particles to Continuum: Modelling and Computation Modelling Muscle Contraction a multiscale approach Giovanni Naldi Dipartimento di Matematica ``F. Enriques

More information

Lab 1 Uniform Motion - Graphing and Analyzing Motion

Lab 1 Uniform Motion - Graphing and Analyzing Motion Lab 1 Uniform Motion - Graphing and Analyzing Motion Objectives: < To observe the distance-time relation for motion at constant velocity. < To make a straight line fit to the distance-time data. < To interpret

More information

High resolution THz scanning for optimization of dielectric layer opening process on doped Si surfaces

High resolution THz scanning for optimization of dielectric layer opening process on doped Si surfaces High resolution THz scanning for optimization of dielectric layer opening process on doped Si surfaces P. Spinelli 1, F.J.K. Danzl 1, D. Deligiannis 1,2, N. Guillevin 1, A.R. Burgers 1, S. Sawallich 3,

More information

Supplemental material for Bound electron nonlinearity beyond the ionization threshold

Supplemental material for Bound electron nonlinearity beyond the ionization threshold Supplemental material for Bound electron nonlinearity beyond the ionization threshold 1. Experimental setup The laser used in the experiments is a λ=800 nm Ti:Sapphire amplifier producing 42 fs, 10 mj

More information

Supporting Information. Even Hard Sphere Colloidal Suspensions Display. Fickian yet Non-Gaussian Diffusion

Supporting Information. Even Hard Sphere Colloidal Suspensions Display. Fickian yet Non-Gaussian Diffusion Supporting Information Even Hard Sphere Colloidal Suspensions Display Fickian yet Non-Gaussian Diffusion Juan Guan, a Bo Wang, a and Steve Granick a,b,c,* Departments of Materials Science, a Chemistry,

More information

Table of Contents. Chapter Preview. 4.1 Photosynthesis. 4.2 Respiration. 4.3 Cell Division. 4.4 Cell Differentiation

Table of Contents. Chapter Preview. 4.1 Photosynthesis. 4.2 Respiration. 4.3 Cell Division. 4.4 Cell Differentiation Table of Contents Chapter Preview 4.1 Photosynthesis 4.2 Respiration 4.3 Cell Division 4.4 Cell Differentiation Chapter Preview Questions 1. All living things are made of a. tissues. b. muscles. c. cells.

More information

Chapter 10. Mitosis and Cytokinesis

Chapter 10. Mitosis and Cytokinesis Chapter 10. Mitosis and Cytokinesis Mitosis is nuclear division. In the process daughter molecules of DNA are precisely segregated into two new daughter nuclei. Mitosis is usually associated with cell

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION DOI: 10.1038/ncb2647 Figure S1 Other Rab GTPases do not co-localize with the ER. a, Cos-7 cells cotransfected with an ER luminal marker (either KDEL-venus or mch-kdel) and mch-tagged human Rab5 (mch-rab5,

More information

Keystone Exams: Algebra

Keystone Exams: Algebra KeystoneExams:Algebra TheKeystoneGlossaryincludestermsanddefinitionsassociatedwiththeKeystoneAssessmentAnchorsand Eligible Content. The terms and definitions included in the glossary are intended to assist

More information

Lecture 19. A Sieve Plate with large Sieve Pores. Secondary Phloem. Secondary phloem (cont d)

Lecture 19. A Sieve Plate with large Sieve Pores. Secondary Phloem. Secondary phloem (cont d) Lecture 19 Secondary phloem (cont d) Secondary Phloem in Tilia americana (American Basswood) Secondary Phloem of Tilia Stained with Toluidine Blue & viewed with Crossed Polarizers. Secondary Phloem A Sieve

More information

PHY 111L Activity 2 Introduction to Kinematics

PHY 111L Activity 2 Introduction to Kinematics PHY 111L Activity 2 Introduction to Kinematics Name: Section: ID #: Date: Lab Partners: TA initials: Objectives 1. Introduce the relationship between position, velocity, and acceleration 2. Investigate

More information

Chromosome Numbers of Some Common Organisms

Chromosome Numbers of Some Common Organisms Meiosis Introduction Process of reduction division Purpose: Produces gametes (sex ) sperm & egg Meiosis is NOT a cycle like mitosis. 1 2 3 Diploid vs. Haploid Diploid a cell that contains homologous chromosomes

More information

Actinobacteria Relative abundance (%) Co-housed CD300f WT. CD300f KO. Colon length (cm) Day 9. Microscopic inflammation score

Actinobacteria Relative abundance (%) Co-housed CD300f WT. CD300f KO. Colon length (cm) Day 9. Microscopic inflammation score y groups y individuals 9 Actinobacteria Relative abundance (%) acteroidetes Cyanobacteria Deferribacteres Firmicutes Proteobacteria TM Tenericutes Unclassified CDf CDf Co-housed CDf Co-housed CDf CDf CDf

More information

Supplementary Data Supplementary figures

Supplementary Data Supplementary figures Electronic Supplementary Material (ESI) for Biomaterials Science. This journal is The Royal Society of Chemistry 2018 Supplementary Data Supplementary figures Figure S1 - Evaluation of functional motor

More information

Data Science Unit. Global DTM Support Team, HQ Geneva

Data Science Unit. Global DTM Support Team, HQ Geneva NET FLUX VISUALISATION FOR FLOW MONITORING DATA Data Science Unit Global DTM Support Team, HQ Geneva March 2018 Summary This annex seeks to explain the way in which Flow Monitoring data collected by the

More information

let s examine pupation rates. With the conclusion of that data collection, we will go on to explore the rate at which new adults appear, a process

let s examine pupation rates. With the conclusion of that data collection, we will go on to explore the rate at which new adults appear, a process Population Dynamics and Initial Population Size (Module website: http://web.as.uky.edu/biology/faculty/cooper/population%20dynamics%20examples%20 with%20fruit%20flies/theamericanbiologyteacher-populationdynamicswebpage.html

More information

10.2 The Process of Cell Division

10.2 The Process of Cell Division 10.2 The Process of Cell Division Lesson Objectives Describe the role of chromosomes in cell division. Name the main events of the cell cycle. Describe what happens during the four phases of mitosis. Describe

More information

Chapter 4. Displaying and Summarizing. Quantitative Data

Chapter 4. Displaying and Summarizing. Quantitative Data STAT 141 Introduction to Statistics Chapter 4 Displaying and Summarizing Quantitative Data Bin Zou (bzou@ualberta.ca) STAT 141 University of Alberta Winter 2015 1 / 31 4.1 Histograms 1 We divide the range

More information

GENERAL SAFETY: Follow your teacher s directions. Do not work in the laboratory without your teacher s supervision.

GENERAL SAFETY: Follow your teacher s directions. Do not work in the laboratory without your teacher s supervision. Name: Bio AP Lab: Cell Division B: Mitosis & Meiosis (Modified from AP Biology Investigative Labs) BACKGROUND: One of the characteristics of living things is the ability to replicate and pass on genetic

More information

Chapter 7: Statistics Describing Data. Chapter 7: Statistics Describing Data 1 / 27

Chapter 7: Statistics Describing Data. Chapter 7: Statistics Describing Data 1 / 27 Chapter 7: Statistics Describing Data Chapter 7: Statistics Describing Data 1 / 27 Categorical Data Four ways to display categorical data: 1 Frequency and Relative Frequency Table 2 Bar graph (Pareto chart)

More information

LS_C2-quizzes, Version: 1 1

LS_C2-quizzes, Version: 1 1 Name: Select the term that best completes the statement. A. cell division B. chromosomes C. cells D. divides E. centromere F. DNA G. chromatids H. nucleus Date: 1. is the chemical code that contains information

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION SUPPLEMENTARY INFORMATION Figure S1. Speckle intensities at different concentrations and additional tests of single-fluorophore imaging. (a) Speckle intensity scatter plots for movies shown in Fig. 1a.

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION SUPPLEMENTARY INFORMATION SUPPLEMENTARY INFORMATION Trilayer graphene is a semimetal with a gate-tuneable band overlap M. F. Craciun, S. Russo, M. Yamamoto, J. B. Oostinga, A. F. Morpurgo and S. Tarucha

More information

Student Exploration: Cell Division

Student Exploration: Cell Division Name: Date: Student Exploration: Cell Division Prior Knowledge Questions (Do these BEFORE using the Gizmo.) 1. What is the purpose of this activity? 2. Cells reproduce by splitting in half, a process called

More information

Mathematical review trigonometry vectors Motion in one dimension

Mathematical review trigonometry vectors Motion in one dimension Mathematical review trigonometry vectors Motion in one dimension Used to describe the position of a point in space Coordinate system (frame) consists of a fixed reference point called the origin specific

More information

Supplementary Information for

Supplementary Information for Supplementary Information for Multi-Functional In Vivo Vascular Imaging Using Near-Infrared II Fluorescence Guosong Hong 1,3, Jerry C. Lee 2,3, Joshua T. Robinson 1, Uwe Raaz 2, Liming Xie 1, Ngan F. Huang

More information

Molecular Modeling and Conformational Analysis with PC Spartan

Molecular Modeling and Conformational Analysis with PC Spartan Molecular Modeling and Conformational Analysis with PC Spartan Introduction Molecular modeling can be done in a variety of ways, from using simple hand-held models to doing sophisticated calculations on

More information

Simultaneous intracellular chloride and ph measurements using a GFPbased

Simultaneous intracellular chloride and ph measurements using a GFPbased nature methods Simultaneous intracellular chloride and ph measurements using a GFPbased sensor Daniele Arosio, Fernanda Ricci, Laura Marchetti, Roberta Gualdani, Lorenzo Albertazzi & Fabio Beltram Supplementary

More information

Spatial Coherence Properties of Organic Molecules Coupled to Plasmonic Surface Lattice Resonances in the Weak and Strong Coupling Regimes

Spatial Coherence Properties of Organic Molecules Coupled to Plasmonic Surface Lattice Resonances in the Weak and Strong Coupling Regimes Spatial Coherence Properties of Organic Molecules Coupled to Plasmonic Surface Lattice Resonances in the Weak and Strong Coupling Regimes Supplemental Material L. Shi, T. K. Hakala, H. T. Rekola, J. -P.

More information

Life in an unusual intracellular niche a bacterial symbiont infecting the nucleus of amoebae

Life in an unusual intracellular niche a bacterial symbiont infecting the nucleus of amoebae Life in an unusual intracellular niche a bacterial symbiont infecting the nucleus of amoebae Frederik Schulz, Ilias Lagkouvardos, Florian Wascher, Karin Aistleitner, Rok Kostanjšek, Matthias Horn Supplementary

More information

Lab 3: Stars, Stars, Stars!

Lab 3: Stars, Stars, Stars! Lab 3: Stars, Stars, Stars! The Hertzsprung-Russell Diagram Today we will learn about the different types of stars and how they are different form one another. Go to http://astro.unl.edu/naap/hr/hr.html.

More information

Quiz 1: Cells and Cell Structures

Quiz 1: Cells and Cell Structures Quiz 1: Cells and Cell Structures 1. Identify the structures in the diagram. (3 marks) 2. List the 3 cell structures not found in animal cells but are found in plants cells. (1 mark) 3. Where is DNA found

More information

Hydrodynamics of Diamond-Shaped Gradient Nanopillar Arrays for Effective. DNA Translocation into Nanochannels. (Supplementary information)

Hydrodynamics of Diamond-Shaped Gradient Nanopillar Arrays for Effective. DNA Translocation into Nanochannels. (Supplementary information) Hydrodynamics of Diamond-Shaped Gradient Nanopillar Arrays for Effective DNA Translocation into Nanochannels (Supplementary information) Chao Wang 1, Robert L. Bruce, Elizabeth A. Duch, Jyotica V. Patel,

More information

Supplemental Information. The Mitochondrial Fission Receptor MiD51. Requires ADP as a Cofactor

Supplemental Information. The Mitochondrial Fission Receptor MiD51. Requires ADP as a Cofactor Structure, Volume 22 Supplemental Information The Mitochondrial Fission Receptor MiD51 Requires ADP as a Cofactor Oliver C. Losón, Raymond Liu, Michael E. Rome, Shuxia Meng, Jens T. Kaiser, Shu-ou Shan,

More information

Chapter 16. Cellular Movement: Motility and Contractility. Lectures by Kathleen Fitzpatrick Simon Fraser University Pearson Education, Inc.

Chapter 16. Cellular Movement: Motility and Contractility. Lectures by Kathleen Fitzpatrick Simon Fraser University Pearson Education, Inc. Chapter 16 Cellular Movement: Motility and Contractility Lectures by Kathleen Fitzpatrick Simon Fraser University Two eukaryotic motility systems 1. Interactions between motor proteins and microtubules

More information

Polar alignment in 5 steps based on the Sánchez Valente method

Polar alignment in 5 steps based on the Sánchez Valente method 1 Polar alignment in 5 steps based on the Sánchez Valente method Compared to the drift alignment method, this one, allows you to easily achieve a perfect polar alignment in just one step. By "perfect polar

More information

Unit 2: Cellular Chemistry, Structure, and Physiology Module 5: Cellular Reproduction

Unit 2: Cellular Chemistry, Structure, and Physiology Module 5: Cellular Reproduction Unit 2: Cellular Chemistry, Structure, and Physiology Module 5: Cellular Reproduction NC Essential Standard: 1.2.2 Analyze how cells grow and reproduce in terms of interphase, mitosis, and cytokinesis

More information

2. is the period of growth and development for a cell. 3. During interphase, most cells go through three stages rapid growth and

2. is the period of growth and development for a cell. 3. During interphase, most cells go through three stages rapid growth and Chapter 5 Lesson 1- General Lesson Outline Directions: Use the words below to fill in the outline of the text from lesson one. If the word is used more than once, it is followed by the number of times

More information

Heather Currinn, Benjamin Guscott, Zita Balklava, Alice Rothnie and Thomas Wassmer*

Heather Currinn, Benjamin Guscott, Zita Balklava, Alice Rothnie and Thomas Wassmer* Online Resources APP controls the formation of PI(3,5)P 2 vesicles through its binding of the PIKfyve complex. Cellular and Molecular Life Sciences Heather Currinn, Benjamin Guscott, Zita Balklava, Alice

More information

Perinatal Mental Health Profile User Guide. 1. Using Fingertips Software

Perinatal Mental Health Profile User Guide. 1. Using Fingertips Software Perinatal Mental Health Profile User Guide 1. Using Fingertips Software July 2017 Contents 1. Introduction... 3 2. Quick Guide to Fingertips Software Features... 3 2.1 Additional information... 3 2.2 Search

More information

Cell Cycle and Mitosis

Cell Cycle and Mitosis Cell Cycle and Mitosis THE CELL CYCLE The cell cycle, or cell-division cycle, is the series of events that take place in a eukaryotic cell between its formation and the moment it replicates itself. These

More information

PHYSIOLOGY CHAPTER 9 MUSCLE TISSUE Fall 2016

PHYSIOLOGY CHAPTER 9 MUSCLE TISSUE Fall 2016 PHYSIOLOGY CHAPTER 9 MUSCLE TISSUE Fall 2016 2 Chapter 9 Muscles and Muscle Tissue Overview of Muscle Tissue types of muscle: are all prefixes for muscle Contractility all muscles cells can Smooth & skeletal

More information

Mixing in Colliding, Ultrasonically Levitated Drops

Mixing in Colliding, Ultrasonically Levitated Drops Mixing in Colliding, Ultrasonically Levitated Drops Supporting information Details of acoustic levitation. Delivering drops into the acoustic levitation zone is easily ignored as a challenging first step

More information

7th 4.2 review. 1. Chromosomes line up single file at the middle of the cell. 2. Two identical nuclei form. 3. Sister chromatids separate.

7th 4.2 review. 1. Chromosomes line up single file at the middle of the cell. 2. Two identical nuclei form. 3. Sister chromatids separate. Match each activity to the correct phase of mitosis. You may use the same answer more than once. a. prophase b. metaphase c. anaphase d. telophase 1. Chromosomes line up single file at the middle of the

More information

4 th Grade Hinojosa Math Vocabulary Words

4 th Grade Hinojosa Math Vocabulary Words Topic 1 Word Definition Picture Digit A symbol used to make numerals. These are the digits 0, 1, 2, 3, 4, 5, 6, 7, 8, 9. Place value The value of where the digit is in the number, such as units(ones),

More information

Zimmerman AP Biology CBHS South Name Chapter 7&8 Guided Reading Assignment 1) What is resolving power and why is it important in biology?

Zimmerman AP Biology CBHS South Name Chapter 7&8 Guided Reading Assignment 1) What is resolving power and why is it important in biology? Zimmerman AP Biology CBHS South Name Chapter 7&8 Guided Reading Assignment 1) What is resolving power and why is it important in biology? 2) How does an electron microscope work and what is the difference

More information

University of Jordan School of Medicine MD Program Curriculum

University of Jordan School of Medicine MD Program Curriculum University of Jordan School of Medicine MD Program Curriculum Course Title: General Histology Course number: 0502111 Credit Hours: 2 credits Academic Year Level: 1st year, 2nd semester 2016/2017 Course

More information

Name: Date: Hour: Unit Four: Cell Cycle, Mitosis and Meiosis. Monomer Polymer Example Drawing Function in a cell DNA

Name: Date: Hour: Unit Four: Cell Cycle, Mitosis and Meiosis. Monomer Polymer Example Drawing Function in a cell DNA Unit Four: Cell Cycle, Mitosis and Meiosis I. Concept Review A. Why is carbon often called the building block of life? B. List the four major macromolecules. C. Complete the chart below. Monomer Polymer

More information

Engineering the spin couplings in atomically crafted spin chains on an elemental superconductor

Engineering the spin couplings in atomically crafted spin chains on an elemental superconductor Engineering the spin couplings in atomically crafted spin chains on an elemental superconductor Kamlapure et al, 1 Supplementary Figures Supplementary Figure 1 Spectroscopy on different chains. a, The

More information

Real-Time Structural Evolution at the Interface of an. Organic Transistor During Thermal Annealing

Real-Time Structural Evolution at the Interface of an. Organic Transistor During Thermal Annealing Electronic Supplementary Material (ESI) for Journal of Materials Chemistry C. This journal is The Royal Society of Chemistry 2014 Electronic Supplementary Information for: Real-Time Structural Evolution

More information

Tabulation means putting data into tables. A table is a matrix of data in rows and columns, with the rows and the columns having titles.

Tabulation means putting data into tables. A table is a matrix of data in rows and columns, with the rows and the columns having titles. 1 Tabulation means putting data into tables. A table is a matrix of data in rows and columns, with the rows and the columns having titles. 2 converting the set of numbers into the form of a grouped frequency

More information

PERFORMANCE OF THE CELLULAR AUTOMATON (CA) SEED FINDER IN TPC TRACKING. Tracking Focus Group. Abstract

PERFORMANCE OF THE CELLULAR AUTOMATON (CA) SEED FINDER IN TPC TRACKING. Tracking Focus Group. Abstract PERFORMANCE OF THE CELLULAR AUTOMATON (CA) SEED FINDER IN TPC TRACKING Tracking Focus Group Abstract We present a summary of performance evaluations of a new track seed finder in STAR TPC tracking. The

More information

Cell Cycle (mitosis and meiosis) Test Review

Cell Cycle (mitosis and meiosis) Test Review Cell Cycle (mitosis and meiosis) Test Review Name: Chapter 10 1. What problems are caused when a cell becomes too large? When a cell becomes too large the cell is strained and has a hard time moving enough

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION Figure S1. Haploid plant produced by centromere-mediated genome elimination Chromosomes containing altered CENH3 in their centromeres (green dots) are eliminated after fertilization in a cross to wild

More information

How to Make or Plot a Graph or Chart in Excel

How to Make or Plot a Graph or Chart in Excel This is a complete video tutorial on How to Make or Plot a Graph or Chart in Excel. To make complex chart like Gantt Chart, you have know the basic principles of making a chart. Though I have used Excel

More information

Mitosis Verses Meiosis

Mitosis Verses Meiosis Mitosis Verses Meiosis Name LT: I can compare mitosis and meiosis using various resources. Standards: 4.1b, 4.1c Visit the following links: https://www.youtube.com/watch?v=f-ldpgefahi https://www.youtube.com/watch?v=vzdmg7ke69g

More information

Chapter 6: A Tour of the Cell

Chapter 6: A Tour of the Cell Chapter 6: A Tour of the Cell 1. The study of cells has been limited by their small size, and so they were not seen and described until 1665, when Robert Hooke first looked at dead cells from an oak tree.

More information

EXPERIMENT 7: ANGULAR KINEMATICS AND TORQUE (V_3)

EXPERIMENT 7: ANGULAR KINEMATICS AND TORQUE (V_3) TA name Lab section Date TA Initials (on completion) Name UW Student ID # Lab Partner(s) EXPERIMENT 7: ANGULAR KINEMATICS AND TORQUE (V_3) 121 Textbook Reference: Knight, Chapter 13.1-3, 6. SYNOPSIS In

More information

Arithmetic with Whole Numbers and Money Variables and Evaluation (page 6)

Arithmetic with Whole Numbers and Money Variables and Evaluation (page 6) LESSON Name 1 Arithmetic with Whole Numbers and Money Variables and Evaluation (page 6) Counting numbers or natural numbers are the numbers we use to count: {1, 2, 3, 4, 5, ) Whole numbers are the counting

More information